FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50722
Sequences flagged as poor quality0
Sequence length300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG18533.6532471117069516No Hit
CAGGAAACAGCTATGACCCATGGTTGGGGAAAAACTCCTCATCTTCATGT8361.6481999921138757No Hit
CAGGAAACAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAACT7801.5377942510153386No Hit
CAGGAAACAGCTATGACCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT5691.121801190804779No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT5060.9975947320689247No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT5000.9857655455226528No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT4980.9818224833405622No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA4840.954221048065928No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA4830.9522495169748827No Hit
CAGGAAACAGCTATGACCGGGACCAGTCCAATAACCCCTGAGGGGTATAT4200.8280430582390284No Hit
CAGGAAACAGCTATGACCTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT4070.8024131540554394No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCTGGGTGCTGGTG4070.8024131540554394No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA4060.8004416229643941No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCTGCCCAACACCC3960.780726312053941No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGGACACTGCCCAC3770.7432672213240803No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC3700.7294665036867631No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGCACTCCTTC3690.7274949725957178No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT3640.7176373171404914No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA3210.6328614802255432No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGACACTGCCCACC3020.5954023894956824No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT2910.573715547494184No Hit
CAGGAAACAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT2890.5697724853120933No Hit
CAGGAAACAGCTATGACCTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT2860.5638578920389574No Hit
CAGGAAACAGCTATGACCGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA2850.5618863609479121No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG2840.5599148298568668No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGCTCCTCCTCGC2760.5441425811285044No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGGCTGTTT2640.5204842080359607No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA2600.5125980836717795No Hit
CAGGAAACAGCTATGACCCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA2420.477110524032964No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT2420.477110524032964No Hit
CAGGAAACAGCTATGACCCTGGCCTCCTTCAATGCATTTAATGGCCTTAT2380.4692243996687827No Hit
CAGGAAACAGCTATGACCTGGAAACCCATGCCATGCACTCAACACTACAA2370.46725286857773746No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG2340.46133827530460153No Hit
CAGGAAACAGCTATGACCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT2320.45739521312251097No Hit
CAGGAAACAGCTATGACCGGCGGGCCCTGATATAGAGCCACTGGCCGTCG2260.4455660265762391No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCATA2220.4376799022120579No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTAGTCTC2210.43570837112101257No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT2170.4278222467568314No Hit
CAGGAAACAGCTATGACCGGGTCCCACCCATGCTGAAATGCGATCCTCAA2170.4278222467568314No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG2070.40810693584637825No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG1860.3667047829344269No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT1850.36473325184338157No Hit
CAGGAAACAGCTATGACCTCCCACCACTGACTCCAGGGGAAGGAGGAGGA1820.3588186585702457No Hit
CAGGAAACAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT1780.3509325342060644No Hit
CAGGAAACAGCTATGACCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG1780.3509325342060644No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA1770.3489610031150191No Hit
CAGGAAACAGCTATGACCTCCTGCCCCACCCTGATACAGGTCACTGTGGA1760.34698947202397384No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT1760.34698947202397384No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC1740.34304640984188317No Hit
CAGGAAACAGCTATGACCCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG1720.3391033476597926No Hit
CAGGAAACAGCTATGACCAAACTATTAATACCTGCAGCAAAGCCCCAAGC1700.335160285477702No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1690.3331887543866567No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCAGGAAACAGCT1650.32530263002247545No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGGAGTCAC1590.31347344347620365No Hit
CAGGAAACAGCTATGACCAGCAACATACCGTTTGTAAACAACTGGCCGTC1560.3075588502030677No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA1480.29178660147470525No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGGCACGGGAACA1440.283900477110524No Hit
CAGGAAACAGCTATGACCGGCAGACAGCCTCTCCTCCTTTCATCACAAAA1400.2760143527463428No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA1360.2681282283821616No Hit
CAGGAAACAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG1340.264185166200071No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCACCCCACCCTGCT1330.2622136351090257No Hit
CAGGAAACAGCTATGACCCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC1320.26024210401798037No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGATTCTCAGATTCC1280.25235597965379913No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC1270.2503844485627538No Hit
CAGGAAACAGCTATGACCTGGCATCCAGGGCCTATTTGGTTCCTATTGAG1270.2503844485627538No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT1260.24841291747170852No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1250.2464413863806632No Hit
CAGGAAACAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGCCCAGACGGC1240.24446985528961793No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT1210.238555262016482No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1200.2365837309254367No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1200.2365837309254367No Hit
CAGGAAACAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG1190.23461219983439136No Hit
CAGGAAACAGCTATGACCAGGGCAAGTGTTCGCGTTGTAGGCGGCGGGAC1180.2326406687433461No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTCCCCAACCATAG1170.23066913765230077No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGCGGGGCGCCACC1150.22672607547021018No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAACAGCAGACACC1130.22278301328811956No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGCAGGGGCATCCGG1110.21883995110602894No Hit
CAGGAAACAGCTATGACCCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG1080.21292535783289301No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCGGAGAGCCTGTG1070.21095382674184773No Hit
CAGGAAACAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGCCCATACGGC1070.21095382674184773No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCCATGCTCACCT1060.20898229565080242No Hit
CAGGAAACAGCTATGACCGCATCCTGCTTAGTGCTGCCCACAGTGTCATT1040.2050392334687118No Hit
CAGGAAACAGCTATGACCAGGTTGCTGGATTCAGTTTGCTCAGGAAACAG1040.2050392334687118No Hit
CAGGAAACAGCTATGACCGCATCCTGCTTAGTGCTGCCTCAATCTGGTCC990.19518157801348526No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGGTCCTCCTC980.19321004692243998No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAGGCACTACGT980.19321004692243998No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT980.19321004692243998No Hit
CAGGAAACAGCTATGACCTGCTGGGAGAGGGGGATGAGTAGACAGGGCCA970.19123851583139465No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGTCTGCAGCCTGG960.18926698474034936No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT940.18532392255825875No Hit
CAGGAAACAGCTATGACCGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG920.18138086037616813No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC920.18138086037616813No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCATCCTGCACGTGG920.18138086037616813No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAGGCCCCTGTACC910.17940932928512285No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAGTAGTCT900.1774377981940775No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCAGGGCAGCGGT900.1774377981940775No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTTCATTCT890.1754662671030322No Hit
CAGGAAACAGCTATGACCTGCCCAATAGTTCTTGCCTCCAAAATTATCCT880.17349473601198692No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGGCTCTTTT880.17349473601198692No Hit
CAGGAAACAGCTATGACCTCCAGCCAGCCAGTCCTTGTGTTGATGGCCTC870.17152320492094159No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT860.1695516738298963No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGACACCCAAGACA860.1695516738298963No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGAAAAGTTTCAGG840.1656086116478057No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA820.16166554946571507No Hit
CAGGAAACAGCTATGACCGGGTCCCACCCATGCTGAAATGTGATCCTCAA810.15969401837466976No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCGTGTTCTCACTC810.15969401837466976No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGTCCTGTGCCCA800.15772248728362445No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGCCCAAGACACTG790.15575095619257917No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC780.15377942510153386No Hit
CAGGAAACAGCTATGACCTCCAGTAAAACTGCCTCTGTTTCAACTCCCTT770.15180789401048855No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCGCCCAACACCC770.15180789401048855No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCATCTGCACCA760.14983636291944324No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCCCCGGGCAGA760.14983636291944324No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTAGTTCC750.14786483182839794No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT750.14786483182839794No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCAGAGCCACC750.14786483182839794No Hit
CAGGAAACAGCTATGACCGATGTAGCAGTGCTCTGGAGCCCTCCCTTGCT750.14786483182839794No Hit
CAGGAAACAGCTATGACCTGCACACACCTCCTATGTTAACAGGCACAAGT740.14589330073735263No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACGGCCCCTCTGCC740.14589330073735263No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTTGCAGTGAGCCG740.14589330073735263No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG730.14392176964630732No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCCAGCTAAGTGA730.14392176964630732No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT730.14392176964630732No Hit
CAGGAAACAGCTATGACCGATGTAGCAGTGCTCTGGAGTCCTCCCTTGCT730.14392176964630732No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAACACACACACAC720.141950238555262No Hit
CAGGAAACAGCTATGACCTCCCTGCTTGATAATGACCCCTCTGAATTCTT720.141950238555262No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACCCTTTGTCCA720.141950238555262No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACACAGCTGGGAGGC710.1399787074642167No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTTGGCATGAAGG710.1399787074642167No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGGCTGCTT710.1399787074642167No Hit
CAGGAAACAGCTATGACCGGGCAGGCCGAGGACATTAGAAATTTGGGACG700.1380071763731714No Hit
CAGGAAACAGCTATGACCCCCTGCGGCTCCTTTCAAGTTAAGTCACCACT680.1340641141910808No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTACTCCCAACCA680.1340641141910808No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT670.1320925831000355No Hit
CAGGAAACAGCTATGACCGTGCTCCTTGGGGACGTAGTCGCCTGGGGACT650.12814952091794488No Hit
CAGGAAACAGCTATGACCTTGGACAGCACCTTGAATGCTATCAGGGAAGC640.12617798982689957No Hit
CAGGAAACAGCTATGACCAGGTTGCTGGATTCAGTTTGCTGACCAACTTT620.12223492764480896No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT620.12223492764480896No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTCCCTTTGGGCT620.12223492764480896No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAGGGGACATGGCC620.12223492764480896No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACGGGGATGGCAGTG610.12026339655376364No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCTCTGCAGCTAGC600.11829186546271835No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCTGAGCAGTGGGG590.11632033437167305No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGCCTCCTGGGTTCA590.11632033437167305No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC590.11632033437167305No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGTGCCACTGCACTGCA590.11632033437167305No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTACTGGCATGAA580.11434880328062774No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAATGAAACTTGGTT580.11434880328062774No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGACACACCACT570.11237727218958242No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGCCCTGAGGGCCC570.11237727218958242No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCCGCTCAGCTCC560.11040574109853712No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTGCAGTGAACTGA560.11040574109853712No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGCATCAGTGCTTC550.10843421000749183No Hit
CAGGAAACAGCTATGACCTTCCTGATGGGATTCAGCCATCAACAGTAGGA550.10843421000749183No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGCTGTCCTCCTC540.10646267891644651No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTAGTCCC540.10646267891644651No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCCTCTCCCAACT540.10646267891644651No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGGTACCCGTGTC530.10449114782540121No Hit
CAGGAAACAGCTATGACCGTCAACAGCATACCTGTACACCTGAAATAACT530.10449114782540121No Hit
CAGGAAACAGCTATGACCAGCGTCTCATTCCATAAAGGCCACCATGAAAC520.1025196167343559No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCAAGTGCTGATGTA510.10054808564331058No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)