FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163834
Sequences flagged as poor quality0
Sequence length300
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC17821.087686316637572No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT16511.0077273337646642No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC15790.9637804118803179No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT15520.9473003161736879No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT14910.9100675073550057No Hit
CAGGAAANAGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA13630.8319396462272788No Hit
CAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC12500.7629673938254575No Hit
CAGGAAANAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT12240.747097672033888No Hit
CAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC11980.7312279502423185No Hit
CAGGAAANAGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT11870.7245138371766544No Hit
CAGGAAANAGCTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC11690.7135271067055678No Hit
CAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG10050.6134257846356678No Hit
CAGGAAANAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT9880.6030494280796416No Hit
CAGGAAANAGCTATGACCAGGCAGTAACTTGGGCATTTCTTCTGATTCTC9880.6030494280796416No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG9760.5957249410989172No Hit
CAGGAAANAGCTATGANNACAAACGCAAATGGCTTTTAGCATTTCACAGG9670.5902315758633739No Hit
CAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA9370.5719203584115629No Hit
CAGGAAANAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA9260.5652062453458989No Hit
CAGGAAANAGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT8780.5359082974230013No Hit
CAGGAAANAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC8730.5328564278476995No Hit
CAGGAAANAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT7930.4840265146428702No Hit
CAGGAAANAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC7830.4779227754922666No Hit
CAGGAAANAGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC7630.46571529719105925No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT7570.46205305370069705No Hit
CAGGAAANAGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT7090.43275510577779946No Hit
CAGGAAANAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC7040.4297032362024976No Hit
CAGGAAANAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT6940.42359949705189404No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT6900.42115800139165255No Hit
CAGGAAANAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG6790.4144438883259885No Hit
CAGGAAANAGCTATGACCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC6680.4077297752603245No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA6600.4028467839398415No Hit
CAGGAAANAGCTATGANNCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC6580.4016260361097208No Hit
CAGGAAANAGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT6520.39796379261935866No Hit
CAGGAAANAGCTATGANNCAGGGTGACTCCGATCCTGGAGGACAAGGCGG6380.3894185578085135No Hit
CAGGAAANAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT6370.3888081838934532No Hit
CAGGAAACAGCTATGACCAGGCAGTAACTTGGGCATTTCTTCTGATTCTC6150.37537995776212507No Hit
CAGGAAANAGCTATGANCAGGCAGTAACTTGGGCATTTCTTCTGATTCTC6090.37171771427176287No Hit
CAGGAAANAGCTATGANCTGTTTTGCCCCTGAAATGAATATTCATCGTCT6080.3711073403567025No Hit
CAGGAAANAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG6040.3686658446964611No Hit
CAGGAAANAGCTATGANNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT5930.361951731630797No Hit
CAGGAAANAGCTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA5840.3564583663952537No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC5780.35279612290489154No Hit
CAGGAAANAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG5740.3503546272446501No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG5730.3497442533295897No Hit
CAGGAAANAGCTATGANNACACCCGGAGCAGTGCAAATCAACTCCCCCAG5610.34241976634886534No Hit
CAGGAAANAGCTATGANNCCAGGAGAGAGCAGCCTTCCTCACACCCGTGA5570.33997827068862385No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA5520.336926401113322No Hit
CAGGAAANAGCTATGANCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT5520.336926401113322No Hit
CAGGAAANAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA5510.33631602719826165No Hit
CAGGAAANAGCTATGANCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA5410.330212288047658No Hit
CAGGAAANAGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG5350.3265500445572958No Hit
CAGGAAANAGCTATGANNTGACAGCACAAGGAACACTGACCTACAGATAA5280.32227742715187324No Hit
CAGGAAANAGCTATGANNTCAAGCAAGTTCAGGATGGTATAGAAATAGAG5250.3204463054066921No Hit
CAGGAAANAGCTATGANNAAGTTGGTCAGCCCTGGTGACTCATTGGATAT5220.31861518366151104No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT5030.3070180792753641No Hit
CAGGAAANAGCTATGANCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC5010.30579733144524335No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC4850.2960313488042775No Hit
CAGGAAANAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA4780.29175873139885494No Hit
CAGGAAANAGCTATGANNGCTGCTGACCCAACCCTACCCTGTCCAGCCCC4720.28809648790849274No Hit
CAGGAAANAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT4710.2874861139934324No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC4690.28626536616331166No Hit
CAGGAAANAGCTATGANCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC4650.2838238705030702No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT4580.2795512530976476No Hit
CAGGAAANAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC4570.27894087918258725No Hit
CAGGAAANAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT4540.2771097574374061No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG4530.2764993835223458No Hit
CAGGAAANAGCTATGANCACAGTGTCCCCATCAGGCTCCGGGACAGCATC4490.27405788786210433No Hit
CAGGAAANAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT4470.2728371400319836No Hit
CAGGAAANAGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC4380.2673437747964403No Hit
CAGGAAACAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG4370.2667334008813799No Hit
CAGGAAANAGCTATGANNCCCAAACCTGCGGAGGCACGGGGATGGCAGTG4350.2655126530512592No Hit
CAGGAAANAGCTATGANNTGGCATCCAGGGCCTATTTGGTTCCTATTGAG4240.2587985399855952No Hit
CAGGAAANAGCTATGANCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG4190.25574667041029336No Hit
CAGGAAANAGCTATGANCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC4130.25208442691993116No Hit
CAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC4070.24842218342956893No Hit
CAGGAAACAGCTATGACCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC3990.243539192109086No Hit
CAGGAAANAGCTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC3850.2349939572982409No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG3780.23072133989281832No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA3750.22889021814763724No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA3750.22889021814763724No Hit
CAGGAAANAGCTATGACCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG3740.22827984423257688No Hit
CAGGAAANAGCTATGANCACAAACGCAAATGGCTTTTAGCATTTCACAGG3680.2246176007422147No Hit
CAGGAAANAGCTATGACCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC3660.22339685291209396No Hit
CAGGAAANAGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC3650.22278647899703358No Hit
CAGGAAANAGCTATGANCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA3520.2148516181012488No Hit
CAGGAAANAGCTATGANCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT3490.2130204963560677No Hit
CAGGAAANAGCTATGACCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA3280.20020264413980002No Hit
CAGGAAANAGCTATGANNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT3280.20020264413980002No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA3260.19898189630967933No Hit
CAGGAAANAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG3220.19654040064943784No Hit
CAGGAAANAGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA3190.19470927890425674No Hit
CAGGAAANAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG3170.193488531074136No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA3160.19287815715907566No Hit
CAGGAAANAGCTATGACCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG3150.19226778324401528No Hit
CAGGAAANAGCTATGACCCCAGGAGAGAGCAGCCTTCCTCACACCCGTGA3140.19165740932895492No Hit
CAGGAAANAGCTATGANCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG3110.18982628758377382No Hit
CAGGAAANAGCTATGANNTGCCAAAATTAAGGATGCACACCTGTGACACA3090.18860553975365307No Hit
CAGGAAANAGCTATGANCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA3060.186774418008472No Hit
CAGGAAACAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT3060.186774418008472No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT3040.18555367017835125No Hit
CAGGAAANAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG3030.1849432962632909No Hit
CAGGAAANAGCTATGANNACCTGGCAGCCCCAAATCTGCACCTCCCGCGG3000.1831121745181098No Hit
CAGGAAANAGCTATGANNGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA2990.18250180060304944No Hit
CAGGAAANAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT2980.18189142668798905No Hit
CAGGAAANAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA2930.17883955711268723No Hit
CAGGAAACAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT2930.17883955711268723No Hit
CAGGAAANAGCTATGANNGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG2910.17761880928256651No Hit
CAGGAAANAGCTATGACCTGACAGCACAAGGAACACTGACCTACAGATAA2860.17456693970726467No Hit
CAGGAAANAGCTATGACCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA2840.17334619187714392No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC2840.17334619187714392No Hit
CAGGAAANAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC2830.1727358179620836No Hit
CAGGAAANAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC2760.168463200556661No Hit
CAGGAAANAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT2760.168463200556661No Hit
CAGGAAANAGCTATGANCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT2680.16358020923617808No Hit
CAGGAAANAGCTATGANCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT2630.16052833966087623No Hit
CAGGAAANAGCTATGANCCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC2590.15808684400063477No Hit
CAGGAAANAGCTATGACCTGGCATCCAGGGCCTATTTGGTTCCTATTGAG2590.15808684400063477No Hit
CAGGAAANAGCTATGANNCAGAAGGCTGAGCAGGAGGCCCTTCCTTACCT2520.1538142265952122No Hit
CAGGAAANAGCTATGANNGTGCTCCTTGGGGACGTAGTCGCCTGGGGACT2520.1538142265952122No Hit
CAGGAAANAGCTATGACCCACGACACCCCACCTCTTGGGACACTGCCCAC2480.15137273093497075No Hit
CAGGAAANAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC2430.14832086135966893No Hit
CAGGAAANAGCTATGANNTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC2380.1452689917843671No Hit
CAGGAAANAGCTATGANCCTGGAATTTCAACAGAATGGCATTTATATGAT2360.14404824395424637No Hit
CAGGAAANAGCTATGANNGCAGCCACCCTGAACACACTTGGCTATCACCC2280.13916525263376345No Hit
CAGGAAANAGCTATGANNCACGACACCCCACCTCTTGGACACTGCCCACC2260.1379445048036427No Hit
CAGGAAACAGCTATGACCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG2250.13733413088858235No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC2250.13733413088858235No Hit
CAGGAAANAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC2220.13550300914340124No Hit
CAGGAAANAGCTATGANCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG2210.13489263522834088No Hit
CAGGAAANAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA2130.13000964390785796No Hit
CAGGAAANAGCTATGANCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA2120.1293992699927976No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG2120.1293992699927976No Hit
CAGGAAANAGCTATGANCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC2110.12878889607773722No Hit
CAGGAAANAGCTATGANCTGACAGCACAAGGAACACTGACCTACAGATAA2070.12634740041749576No Hit
CAGGAAACAGCTATGACCCCAGGAGAGAGCAGCCTTCCTCACACCCGTGA2070.12634740041749576No Hit
CAGGAAANAGCTATGACCCCCAAACCTGCGGAGGCACGGGGATGGCAGTG2040.12451627867231467No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG2040.12451627867231467No Hit
CAGGAAACAGCTATGACCTGACAGCACAAGGAACACTGACCTACAGATAA2030.1239059047572543No Hit
CAGGAAANAGCTATGANNCTCACCAAGAGTGCCCCACGTGGTGGGCCACA2020.12329553084219393No Hit
CAGGAAANAGCTATGANCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG2010.12268515692713355No Hit
CAGGAAACAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG2000.1220747830120732No Hit
CAGGAAANAGCTATGANCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG1990.12146440909701284No Hit
CAGGAAACAGCTATGACCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG1970.1202436612668921No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT1930.11780216560665063No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA1910.1165814177765299No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC1900.11597104386146953No Hit
CAGGAAANAGCTATGANCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT1890.11536066994640916No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG1880.11475029603134881No Hit
CAGGAAANAGCTATGANCACACCCGGAGCAGTGCAAATCAACTCCCCCAG1880.11475029603134881No Hit
CAGGAAANAGCTATGANNGGGCAGGCCGAGGACATTAGAAATTTGGGACG1870.11413992211628844No Hit
CAGGAAANAGCTATGANCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT1840.11230880037110735No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT1830.11169842645604698No Hit
CAGGAAANAGCTATGANNGTGCAGTCCTCAGGCCACACTAGAAGTAATTA1830.11169842645604698No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC1820.11108805254098661No Hit
CAGGAAANAGCTATGANCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG1780.10864655688074515No Hit
CAGGAAANAGCTATGANNGGGTCCCACCCATGCTGAAAGTCCACCTTCCA1770.10803618296568478No Hit
CAGGAAANAGCTATGANNGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC1750.10681543513556405No Hit
CAGGAAANAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA1740.10620506122050369No Hit
CAGGAAANAGCTATGACCACCTGGCAGCCCCAAATCTGCACCTCCCGCGG1730.10559468730544332No Hit
CAGGAAANAGCTATGANNGCTACGGGCAATCACTCTCCTATTCCCAACCA1730.10559468730544332No Hit
CAGGAAANAGCTATGANNTCCCACTTGCTCTTTGAAGGCAATTCCATTCT1730.10559468730544332No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1720.10498431339038294No Hit
CAGGAAANAGCTATGACCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT1720.10498431339038294No Hit
CAGGAAANAGCTATGANCCCAGGAGAGAGCAGCCTTCCTCACACCCGTGA1710.10437393947532259No Hit
CAGGAAANAGCTATGACCCAGAAGGCTGAGCAGGAGGCCCTTCCTTACCT1700.10376356556026221No Hit
CAGGAAANAGCTATGANNGCTACGGGCAATCACTCTCCTACTCCCAACCA1680.10254281773014147No Hit
CAGGAAANAGCTATGANCTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC1680.10254281773014147No Hit
CAGGAAANAGCTATGANCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1640.10010132206990001No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)