FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126638
Sequences flagged as poor quality0
Sequence length300
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCTGGAAACCCATGCCATGCACTCAACACTACAA29392.3207883889511836No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCTGAGGCAGGAGA26302.0767857988913283No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACCCCGTTAGCTGC26262.073627189311265No Hit
CAGGAAACAGCTATGACCACACATACTCCCTTGCTCCAGGGCTGCAGAAC23061.8209384229062369No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT19331.5263980795653753No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC19101.5082360744800138No Hit
CAGGAAACAGCTATGACCTCCCTTAAACTTGGGATTGCTCCATTGCACTC18631.4711224119142754No Hit
CAGGAAACAGCTATGACCGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT17911.414267439473144No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCAGAGCCACC17271.3637296861921382No Hit
CAGGAAACAGCTATGACCCAGGGTGACTCCGATCCTGGAGCCAACGGTGC15531.226330169459404No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCTGCAGTGCGGT14971.1821096353385239No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT14881.1750027637833826No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGGACACTGCCCAC14821.1702648494132883No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGACTGGCCGTCGTT14131.115778834157204No Hit
CAGGAAACAGCTATGACCACCTGGCAGCCCCAAATCTGCGGGTGACCACG13421.0597135141110883No Hit
CAGGAAACAGCTATGACCTCCAGCCAGCCAGTCCTTGTGTTGATGGCCTC12731.005227498855004No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTCCCCAACCATAG12430.9815379270045326No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCACGGGCATCCTG11740.9270519117484484No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCACCCCACCCTGCT11680.922313997378354No Hit
CAGGAAACAGCTATGACCACCTTTACCCAAATAGCTACCACCAGTGCCTT10700.8449280626668141No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGCTCCTCCTCGC10530.8315039719515469No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGACACCCAAGACA10410.8220281432113584No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA10390.820448838421327No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCAAGCCTGACTG10250.8093937048911068No Hit
CAGGAAACAGCTATGACCAGAGGCACAGGTGGAGAGGGACGAGACCCTGA9560.7549076896350226No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG9510.7509594276599441No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACCCTGTCTCTCTAGC9210.7272698558094727No Hit
CAGGAAACAGCTATGACCATCCACTGTTGGCCAACACACCTGGCCTTCAA8970.7083181983290956No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC8610.6798907121085298No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGACACTGCAGCCTGGG8610.6798907121085298No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCTCCCCGCCAAGA8480.6696252309733255No Hit
CAGGAAACAGCTATGACCGATGGCAGAATGGAAACATGGCAGCTGCCTTG8160.6443563543328227No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCCATGCTCACCT8100.6396184399627285No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAACCATGTGCCC7820.6175081729022884No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGACACTGCCCACC7560.5969772106318798No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGCAGGAAACAGCT7410.5851324247066442No Hit
CAGGAAACAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG7210.5693393768063298No Hit
CAGGAAACAGCTATGACCACACATACTCCCTTGCTCCACGTGTGCTGTGG7190.5677600720162984No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGCCCAAGACACTG7150.5646014624362355No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC6610.5219602331053871No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC6580.5195912759203398No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCAGCTCCGA6530.5156430139452612No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA6400.505377532810057No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG6310.49827066125491554No Hit
CAGGAAACAGCTATGACCGGGACCAGTCCAATAACCCCTGAGGGGTATAT6140.48484657053964847No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAACTGGCCAGCCTG6030.47616039419447564No Hit
CAGGAAACAGCTATGACCGCTTCTATATGAGCGCATCTCACCTGAATTTG5940.46905352263933414No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACACAGCTGGGAGGC5900.4658949130592713No Hit
CAGGAAACAGCTATGACCGGCGGGCCCTGATATAGAGCCACTGGCCGTCG5660.44694325557889414No Hit
CAGGAAACAGCTATGACCAGGCAGTAACTTGGGCATTTCTTCTGATTCTC5630.44457429839384704No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCGTGTTCTCACTC5630.44457429839384704No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTGGGGAGGGTGG5570.4398363840237528No Hit
CAGGAAACAGCTATGACCGATGGCAGAATGGAAACATGGCACCTTGCTTC5340.4216743789383913No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAGGCACTACGT5230.4129882025932185No Hit
CAGGAAACAGCTATGACCAGGACTTGCCCACTCTCCCAGGAAGCAGAAGT5180.4090399406181399No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCGTGTGCGCTGGA5160.4074606358281085No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGCCCGGACGAGGA5090.4019330690629984No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG5080.40114341666798276No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCCCCGGGCAGA4980.3932468927178256No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACCAGGAAACAGCT4980.3932468927178256No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA4850.38298141158262133No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACCCTTTGTCCA4800.37903314960754275No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCTTCCCATCTCA4770.37666419242249566No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC4480.3537642729670399No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACCACCGTGATCTCTC4450.35139531578199273No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGACACACCACT4280.33797122506672567No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCTCCGTGTGCGCT4200.33165400590659994No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT4140.32691609153650564No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCCTCTCCCAACT4140.32691609153650564No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTGGCAGCTGCAC4050.31980921998136425No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTCCCTTTGGGCT3970.3134920008212385No Hit
CAGGAAACAGCTATGACCAGAGGCACAGGTGGAGAGGGGGTGATGGCCAT3920.30954373884615993No Hit
CAGGAAACAGCTATGACCCCAGCAGAGCTCTTCTTACAGAGGCAGGAAAC3890.3071747816611128No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGCATCCTGG3860.30480582447606563No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCCTGCCCCCTGCA3860.30480582447606563No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGATGCCACG3730.2945403433408613No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGTCTGCAGCCTGG3710.29296103855082994No Hit
CAGGAAACAGCTATGACCCCGTAGCCACGCCCCTATTGTCTGACCACGCT3650.28822312418073565No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTCCCTTTGGGC3560.28111625262559425No Hit
CAGGAAACAGCTATGACCTGCTTTGGGGCCATGGAGTTGAGCTGTTGAGA3490.2755886858604842No Hit
CAGGAAACAGCTATGACCGAAAGGCTTCCCCAGGAGCCTCAGCAGTGTGG3480.2747990334654685No Hit
CAGGAAACAGCTATGACCGGAAATAAGTACCTTCTGTGTCCTTGTATGTC3460.2732197286754371No Hit
CAGGAAACAGCTATGACCACCCAGTTAGAGCCATGGTTCCTGTAGGCTAG3290.2597956379601699No Hit
CAGGAAACAGCTATGACCTCCCTCATTGTTGAGAGGCAGCAGTTGCAGAA3190.2518991140100128No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGTGGACTAGGTCC3180.2511094616149971No Hit
CAGGAAACAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT3170.25031980921998137No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGTCTGGGTCCT3120.2463715472449028No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTGCCATGCTCACC3090.24400259005985564No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGTCCTGTGCCCA3020.23847502329474565No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGCCTGCCCAGGCC2990.23610606610969853No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCCGTGTGCGCTGG2960.23373710892465138No Hit
CAGGAAACAGCTATGACCAACCAACACTGCTGCTAATTGTGTTTGTGTCC2930.23136815173960423No Hit
CAGGAAACAGCTATGACCGGAAATAAGTACCTTCTGTGTCCTTGTGGCAG2920.2305784993445885No Hit
CAGGAAACAGCTATGACCATCCACTGTTGGCCAACACAGGGGCGATAGTG2890.22820954215954137No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGGCACGGGAACA2770.2187337134193528No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTTGGGCCAGGGT2720.21478545144427422No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTGACCCTGGCAG2670.21083718946919566No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCCGGGCAGAGG2610.2060992750991014No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCAGATTTTCAAT2580.20373031791405424No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG2550.20136136072900707No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT2530.19978205593897566No Hit
CAGGAAACAGCTATGACCCCCGTGACAGCTCCTGCAATTACAAAATGGCT2350.1855683128286928No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCGTGCTGGGCCA2330.1839890080386614No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTAGTGGCATGAA2320.18319935564364567No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTGGCATGAAGGA2290.18083039845859852No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGCCTGGCTTGTC2280.1800407460635828No Hit
CAGGAAACAGCTATGACCCTGGGTGCCGGGTAGAGTGTCAGGAAACAGTC2270.17925109366856712No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCGGGCATCCT2240.17688213648351994No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCTGGAGTGCAGT2220.17530283169348854No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC2190.1729338745084414No Hit
CAGGAAACAGCTATGACCGGCCTTCTCTGCATTATCTCGCTAATCTCGAG2190.1729338745084414No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACGGGGATGGCAGTG2180.17214422211342567No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCAAGAGGGGGCAG2110.16661665534831566No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCTGGAGGGCAGT2040.16108908858320567No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGGGGGCCTGGCT2020.15950978379317424No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG2020.15950978379317424No Hit
CAGGAAACAGCTATGACCGAAAGGCTTCCCCAGGAGCCTCCCTTAGGAGT2020.15950978379317424No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCCCACAGAGAGCAC2010.15872013139815855No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCTGTCACACACCC2000.15793047900314283No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCACGCCCGGCCT1960.15477186942307997No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGTCACTGCAGCCTGGG1950.15398221702806425No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTACCATGCTCACC1940.15319256463304853No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGTCTGTGGGCGGA1930.15240291223803282No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT1880.14845465026295424No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCACCCCCCCATC1860.14687534547292283No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCACAGGAAACAGC1810.14292708349784425No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGAACAAAAAGCCTCAC1800.14213743110282853No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATCCAGCAGCAGAG1770.13976847391778138No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCCCGTGTGCGCTG1770.13976847391778138No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGCTGTCCTCCTC1750.13818916912774995No Hit
CAGGAAACAGCTATGACCTGCTTTGGGGCCATGGAGTTGGGGGAATGGGA1670.13187194996762425No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTTGGAGTCCCAC1660.13108229757260853No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTGTAATCCCAGC1660.13108229757260853No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTGAGGAGGGTGG1610.12713403559752998No Hit
CAGGAAACAGCTATGACCACCTTTACCCAAATAGCTACCACCACTGGCAA1610.12713403559752998No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCACCCCAGCAAGA1590.12555473080749854No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCAGCTCTGA1530.12081681643740425No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCAGGCAAACTTGG1530.12081681643740425No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTAGGGTCTGGGGC1520.12002716404238853No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG1500.11844785925235711No Hit
CAGGAAACAGCTATGACCCCAGCAGAGCTCTTCTTACAGAGGTGAAGCAC1470.11607890206730997No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTCACACCTGTAA1460.11528924967229424No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC1460.11528924967229424No Hit
CAGGAAACAGCTATGACCTCCAGCCAGCCAGTCCTTGTCCTTATATCTTT1450.11449959727727856No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCTGTGCTCACCGT1450.11449959727727856No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTGATCAGGGTGG1410.11134098769721569No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT1410.11134098769721569No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAAACCCCATCTCTA1400.11055133530219997No Hit
CAGGAAACAGCTATGACCTGGAGCCAATTTCATGAGGGCACAGGAAACAG1380.10897203051216856No Hit
CAGGAAACAGCTATGACCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG1380.10897203051216856No Hit
CAGGAAACAGCTATGACCAGGACTTGCCCACTCTCCCACAGGAAGCAGAA1370.10818237811715282No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC1360.10739272572213711No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCCAGAGAGCACCCG1350.1066030733271214No Hit
CAGGAAACAGCTATGACCGGGCCTTCTCGAAGCTCTCCCAGGAAACAGCT1350.1066030733271214No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTACTGGCATGAA1340.10581342093210569No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG1330.10502376853708997No Hit
CAGGAAACAGCTATGACCTGCCCAGTTGTTTCCAGAACCTAGCAGAGTCC1330.10502376853708997No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTAGGGGCTGGTC1310.10344446374705854No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGGGCGCAGCCACC1290.10186515895702712No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)