FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119352
Sequences flagged as poor quality0
Sequence length300
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT27422.2974059923587373No Hit
CAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT27212.279810979288156No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG16041.3439238554862927No Hit
CAGGAAANAGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT14371.2040016086869092No Hit
CAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT13801.1562437160667605No Hit
CAGGAAANAGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT12791.071620081774918No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA11250.9425899859239896No Hit
CAGGAAANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT11240.9417521281587238No Hit
CAGGAAANAGCTATGANNCCCAAACCTGCGGAGGCACGGGGATGGCAGTG9660.8093706012467323No Hit
CAGGAAANAGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG9070.759936993096052No Hit
CAGGAAANAGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG8990.7532341309739258No Hit
CAGGAAANAGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT8780.7356391179033447No Hit
CAGGAAANAGCTATGANNACTCCCAACTCCCGTCTGGTCGGGGCAAAAAC8510.713016958241169No Hit
CAGGAAANAGCTATGANNAGCGTCTCATTCCATAAAGGCCACCATGAAAC7960.6669347811515517No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG7910.6627454923252228No Hit
CAGGAAANAGCTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA7740.6485019103157048No Hit
CAGGAAANAGCTATGANNGGAGGCTGAGGTGGGAGGATCGCTTGATCCCG7550.6325826127756552No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA7350.6158254574703399No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG6970.5839868623902406No Hit
CAGGAAANAGCTATGANNGTCCTAGCACCGCAGCGAATGGGACCCGCAAG6950.5823111468597091No Hit
CAGGAAANAGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG6610.553823982840673No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCCAAACCAG6490.5437696896574837No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC6420.5379046853006233No Hit
CAGGAAANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG6330.5303639654132315No Hit
CAGGAAANAGCTATGANNGCTTCTCGGCCATGTGGGTTAAACTCGGGGAT6050.5069039479857899No Hit
CAGGAAANNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT5310.4449024733561231No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG4840.40552315838863195No Hit
CAGGAAANNGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT4710.39463100744017693No Hit
CAGGAAANNGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA4410.36949527448220393No Hit
CAGGAAANAGCTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC4180.3502245458810912No Hit
CAGGAAANNGCTATGANNCCCAAACCTGCGGAGGCACGGGGATGGCAGTG3890.32592667068838393No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA3800.318385950800992No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA3760.3150345197399289No Hit
CAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA3700.3100073731483344No Hit
CAGGAAANAGCTATGANNACCTGGCAGCCCCAAATCTGCACCTCCCGCAG3590.3007909377304109No Hit
CAGGAAANNGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT3560.29827736443461356No Hit
CAGGAAANNGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG3550.29743950666934776No Hit
CAGGAAANNGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA3500.29325021784301897No Hit
NAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3310.27733092030296935No Hit
CAGGAAANAGCTATGANNACCTGGCAGCCCCAAATCTGCACCTCCCGCGG3270.2739794892419063No Hit
CAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3210.26895234265031165No Hit
CAGGAAANNGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG3130.26224948052818553No Hit
CAGGAAANNGCTATGANNGGAGGCTGAGGTGGGAGGATCGCTTGATCCCG3100.2597359072323882No Hit
CAGGAAANNGCTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA3020.2530330451102621No Hit
CAGGAAANNGCTATGANNAGCGTCTCATTCCATAAAGGCCACCATGAAAC2990.25051947181446477No Hit
NAGGAAANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2960.24800589851866747No Hit
CAGGAAANNGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG2880.24130303639654133No Hit
NAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2860.2396273208660098No Hit
CAGGANANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2850.238789463100744No Hit
CAGGAAANAGCTATGANNCAGGGTGACTCCGATCCTGGAGGACAAGGCGG2850.238789463100744No Hit
CAGGAAANAGCTATGANNCTTGAGCTCCTCGGACACGGCGGCCTTGCTGA2830.2371137475702125No Hit
CAGGAAANAGCTATGANNCCTGGACGCAATCACCCAGGCCAGCTAAGTGA2740.22957302768282056No Hit
CAGGAAANAGCTATGANNGCCCCTACTCCAAAAGGCGGATGAATGGGGAA2690.2253837388564917No Hit
CAGGAAANAGCTATGANNTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG2680.22454588109122597No Hit
CAGGAAANNGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG2670.2237080233259602No Hit
CAGGAAANNGCTATGANNACTCCCAACTCCCGTCTGGTCGGGGCAAAAAC2650.22203230779542865No Hit
NAGGANANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2630.22035659226489712No Hit
CAGGAAANAGCTATGANNAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT2620.21951873449963136No Hit
CAGGAAANAGCTATGANNTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG2620.21951873449963136No Hit
CAGGAAACNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2550.21365373014277095No Hit
CAGGAAANNGCTATGANNGTCCTAGCACCGCAGCGAATGGGACCCGCAAG2530.21197801461223942No Hit
CAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATCCAAACCAG2460.20611301025537906No Hit
CAGGAAANNGCTATGANNGCTTCTCGGCCATGTGGGTTAAACTCGGGGAT2330.19522085930692407No Hit
CAGGAAACAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2310.19354514377639254No Hit
CAGGAAANAGCTATGANNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG2280.19103157048059521No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT2250.18851799718479792No Hit
CAGGAAANAGCTATGANNCAGAAGGCTGAGCAGGAGGCTGGGAAAAAGGG2240.18768013941953213No Hit
CAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2220.1860044238890006No Hit
CAGGAANNNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2110.17678798847107713No Hit
CAGGAAANNGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG2110.17678798847107713No Hit
CAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG1910.16003083316576178No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA1850.1550036865741672No Hit
NAGGAAANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1770.14830082445204104No Hit
CAGGAAANAGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT1770.14830082445204104No Hit
CAGGAAACNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1760.14746296668677525No Hit
CAGGAAANAGCTATGANNCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT1760.14746296668677525No Hit
NAGGANANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1750.14662510892150948No Hit
CAGGAAACAGCTATGANCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1740.14578725115624372No Hit
CAGGAANNNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1720.1441115356257122No Hit
CAGGANANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1670.13992224679938334No Hit
CAGGAAANAGCTATGANNGGTAGCAACGTCCCACCCAGCTGAATAGTTGT1660.13908438903411757No Hit
CAGGAAANAGCTATGANNTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC1640.13740867350358604No Hit
CAGGAAANAGCTATGANNTGACTGCAAACATAGAAGTGAGCAGAGCAAGA1640.13740867350358604No Hit
CAGGAAANNGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1640.13740867350358604No Hit
CAGGAAANNGCTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC1640.13740867350358604No Hit
NAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1630.13657081573832025No Hit
NAGGAAANNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1630.13657081573832025No Hit
CAGGAAANNGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA1630.13657081573832025No Hit
NAGGANANNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1590.1332193846772572No Hit
CAGGAAANAGCTATGANNAGGCTTTGTGTGTGGGGACTGTAATCCCAGCT1580.13238152691199143No Hit
CAGGAAANAGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG1570.13154366914672566No Hit
CAGGAAANAGCTATGANNTCCCTTAAACTTGGGATTGCTCCATTGCACTC1550.1298679536161941No Hit
CAGGAAANAGCTATGANNAGGCTTTGTGTGTGGGGACTTGTAATCCCATC1550.1298679536161941No Hit
CAGGAAANAGCTATGANNATCGCACCACTGCACTCCAGCACTGGCCGTCG1510.12651652255513104No Hit
NAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1470.12316509149406796No Hit
CAGGAAANAGCTATGANNTGACAGCACAAGGAACACTGACCGAGGCAGGA1460.1223272337288022No Hit
CAGGAAANNGCTATGANNCTTGAGCTCCTCGGACACGGCGGCCTTGCTGA1420.11897580266773912No Hit
NAGGAAANAGCTATGANCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1410.11813794490247335No Hit
CAGGANANNGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT1400.11730008713720759No Hit
CAGGAAANNGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1360.11394865607614452No Hit
CAGGANANNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1350.11311079831087874No Hit
NAGGAAANNGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT1330.11143508278034721No Hit
NAGGANANNGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT1260.10557007842348684No Hit
CAGGAAANAGCTATGANNACTCCTCCTGGCCTTCCTCTTCCTCCTGAACC1250.10473222065822106No Hit
NAGGAAANAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1240.10389436289295528No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)