FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82487
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT9211.1165395759331773No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT6970.8449816334695165No Hit
CAGGAAANAGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC5890.7140519112102512No Hit
CAGGAAANAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT5850.7092026622376859No Hit
CAGGAAANAGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT5720.6934426030768485No Hit
CAGGAAANAGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT5600.6788948561591523No Hit
CAGGAAANAGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT5560.674045607186587No Hit
CAGGAAANAGCTATGACCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT5480.6643471092414562No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG5270.638888552135488No Hit
CAGGAAANAGCTATGANNGCTTCTATATGAGCGCATCTCACCTGAATTTG5010.607368433813813No Hit
CAGGAAANAGCTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT4870.5903960624098343No Hit
CAGGAAACAGCTATGACCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT4350.5273558257664844No Hit
CAGGAAANAGCTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA4190.5079588298762229No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT4070.4934110829585268No Hit
CAGGAAANAGCTATGANNAACCAACACTGCTGCTAATTGTGTTTGTGTCC3990.4837125850133961No Hit
CAGGAAANAGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG3990.4837125850133961No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT3900.472801774825124No Hit
CAGGAAANAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT3860.4679525258525586No Hit
CAGGAAANAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAACT3840.4655279013662759No Hit
CAGGAAANAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAATT3740.4534047789348625No Hit
CAGGAAANAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC3510.4255215973426115No Hit
CAGGAAANAGCTATGANCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT3350.4061246014523501No Hit
CAGGAAANAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3100.37581679537381646No Hit
CAGGAAANAGCTATGACCGCTTCTATATGAGCGCATCTCACCTGAATTTG3080.3733921708875338No Hit
CAGGAAANAGCTATGANNGTCCTAGCACCGCAGCGAATGGGACCCGCAAG2930.3552074872404136No Hit
CAGGAAANAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2930.3552074872404136No Hit
CAGGAAANAGCTATGACCTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT2910.35278286275413095No Hit
CAGGAAANAGCTATGANNTCCCACCACTGACTCCAGGGGAAGGAGGAGGA2880.34914592602470695No Hit
CAGGAAACAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAATT2760.3345981791070108No Hit
CAGGAAANAGCTATGANNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT2650.321262744432456No Hit
CAGGAAACAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAACT2620.317625807703032No Hit
CAGGAAANAGCTATGANNTCCTCTTCATTTTCACGGTGCCATTCTGATTC2590.313988870973608No Hit
CAGGAAANAGCTATGANNGCAAAACACCTTAACACTCGGCTTCTATTTGC2590.313988870973608No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG2490.30186574854219456No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCCAAACCAG2490.30186574854219456No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA2490.30186574854219456No Hit
CAGGAAANAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT2470.29944112405591183No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT2450.29701649956962917No Hit
CAGGAAANAGCTATGANNTCCAGTGGTGGGGCTCAACAAAGGCTCCGTGA2400.29095493835392244No Hit
CAGGAAANAGCTATGANCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2360.28610568938135705No Hit
CAGGAAANAGCTATGANNGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT2360.28610568938135705No Hit
CAGGAAANAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG2320.28125644040879166No Hit
CAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA2320.28125644040879166No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2280.27640719143622633No Hit
CAGGAAANAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA2240.27155794246366094No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG2220.2691333179773782No Hit
CAGGAAANAGCTATGACCAACCAACACTGCTGCTAATTGTGTTTGTGTCC2200.2667086934910956No Hit
CAGGAAACAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT2180.2642840690048129No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG2120.2570101955459648No Hit
CAGGAAANAGCTATGANCGCATCAGATTCACTTCCACTGTCGTCAGAACT2110.2557978833028235No Hit
CAGGAAANAGCTATGANNAGGGCAAGTGTTCGCGTTGTAGGCGGCGGGAC2100.25458557105968216No Hit
CAGGAAANAGCTATGANCTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT1980.24003782414198602No Hit
CAGGAAANAGCTATGANNAAGTTGGTCAGCCCTGGTGACTCATTGGATAT1960.23761319965570332No Hit
CAGGAAACAGCTATGACCGCTTCTATATGAGCGCATCTCACCTGAATTTG1930.2339762629262793No Hit
CAGGAAANAGCTATGACCTGTTTTGCCCCTGAAATGAATATCCAAACCAG1920.23276395068313796No Hit
CAGGAAANAGCTATGANCGCATCAGATTCACTTCCACTGTCGTCAGAATT1920.23276395068313796No Hit
CAGGAAANAGCTATGANCCTGGAATTTCAACAGAATGGCATTTATATGAT1870.22670238946743124No Hit
CAGGAAANAGCTATGACCTCCTCTTCATTTTCACGGTGCCATTCTGATTC1870.22670238946743124No Hit
CAGGAAACAGCTATGACCAACCAACACTGCTGCTAATTGTGTTTGTGTCC1840.2230654527380072No Hit
CAGGAAANAGCTATGANCGCTTCTATATGAGCGCATCTCACCTGAATTTG1840.2230654527380072No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1820.2206408282517245No Hit
CAGGAAANAGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT1800.21821620376544182No Hit
CAGGAAANAGCTATGANCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1770.21457926703601782No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA1760.21336695479287643No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT1760.21336695479287643No Hit
CAGGAAANAGCTATGACCGTCCTAGCACCGCAGCGAATGGGACCCGCAAG1750.2121546425497351No Hit
CAGGAAACAGCTATGACCTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT1730.2097300180634524No Hit
CAGGAAANAGCTATGACCTCCCACCACTGACTCCAGGGGAAGGAGGAGGA1680.2036684568477457No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG1650.20003152011832168No Hit
CAGGAAANAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC1640.19881920787518034No Hit
CAGGAAANAGCTATGANNACCAAGACCCCAGCAGCAGAGGGAAGGTGACT1630.19760689563203898No Hit
CAGGAAANAGCTATGANCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT1630.19760689563203898No Hit
CAGGAAANAGCTATGACCTGTTTTGCCCCTGAAATGAATATCTAAACCAG1630.19760689563203898No Hit
CAGGAAANAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1560.1891207099300496No Hit
CAGGAAANAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG1530.18548377320062556No Hit
CAGGAAANAGCTATGANNTGACAGCACAAGGAACACTGACCTACAGATAA1520.1842714609574842No Hit
CAGGAAANAGCTATGACCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT1520.1842714609574842No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1510.18305914871434287No Hit
CAGGAAANAGCTATGANNGCCCCTACTCCAAAAGGCGGATGAATGGGGAA1510.18305914871434287No Hit
CAGGAAANAGCTATGANCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG1500.18184683647120153No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTATGTGCA1440.17457296301235348No Hit
CAGGAAANAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1430.17336065076921212No Hit
CAGGAAANAGCTATGACCTCCAGTGGTGGGGCTCAACAAAGGCTCCGTGA1410.17093602628292945No Hit
CAGGAAANAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG1400.1697237140397881No Hit
CAGGAAANAGCTATGANNAAACTATTAATACCTGCAGCAAAGCCCCAAGC1380.1672990895535054No Hit
CAGGAAANAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT1370.16608677731036406No Hit
CAGGAAANAGCTATGANCAACCAACACTGCTGCTAATTGTGTTTGTGTCC1360.16487446506722273No Hit
CAGGAAANAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT1360.16487446506722273No Hit
CAGGAAANAGCTATGANNGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG1320.16002521609465734No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCTAAACCAG1280.15517596712209195No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC1260.15275134263580928No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCCAAACCAG1250.15153903039266794No Hit
CAGGAAACAGCTATGACCTCCCACCACTGACTCCAGGGGAAGGAGGAGGA1240.15032671814952658No Hit
CAGGAAANAGCTATGANCTCCCACCACTGACTCCAGGGGAAGGAGGAGGA1230.14911440590638525No Hit
CAGGAAANAGCTATGANCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA1230.14911440590638525No Hit
CAGGAAANAGCTATGANNGCTGCTGACCCAACCCTACCCTGTCCAGCCCC1210.14668978142010256No Hit
CAGGAAANAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT1210.14668978142010256No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA1200.14547746917696122No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1140.13820359571811316No Hit
CAGGAAANAGCTATGANNCCCTGCGGCTCCTTTCAAGTTAAGTCACCACT1120.13577897123183047No Hit
CAGGAAANAGCTATGACCTGACAGCACAAGGAACACTGACCTACAGATAA1100.1333543467455478No Hit
CAGGAAANAGCTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC1070.12971741001612375No Hit
CAGGAAANAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1070.12971741001612375No Hit
CAGGAAANAGCTATGANNCCTGGACGCAATCACCCAGGCCAGCTAAGTGA1070.12971741001612375No Hit
CAGGAAANAGCTATGANCTGTTTTGCCCCTGAAATGAATATCCAAACCAG1070.12971741001612375No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT1060.1285050977729824No Hit
CAGGAAANAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT1050.12729278552984108No Hit
CAGGAAANAGCTATGACCACCAAGACCCCAGCAGCAGAGGGAAGGTGACT1050.12729278552984108No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1040.12608047328669972No Hit
CAGGAAANAGCTATGANNCAGAAGGCTGAGCAGGAGGCTGGGAAAAAGGG1040.12608047328669972No Hit
CAGGAAANAGCTATGACCAGGGCAAGTGTTCGCGTTGTAGGCGGCGGGAC1040.12608047328669972No Hit
CAGGAAACAGCTATGACCGTCCTAGCACCGCAGCGAATGGGACCCGCAAG1030.12486816104355838No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC1030.12486816104355838No Hit
CAGGAAACAGCTATGACCTCCTCTTCATTTTCACGGTGCCATTCTGATTC1020.12365584880041702No Hit
CAGGAAANAGCTATGANCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT1010.1224435365572757No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG1010.1224435365572757No Hit
CAGGAAANAGCTATGANNTGTTGTCTTTCAGGGGCCGCCATGCCTGCCGT1000.12123122431413436No Hit
CAGGAAANAGCTATGACCAAACTATTAATACCTGCAGCAAAGCCCCAAGC980.11880659982785166No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT980.11880659982785166No Hit
CAGGAAANAGCTATGANCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT980.11880659982785166No Hit
CAGGAAANAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA980.11880659982785166No Hit
CAGGAAANAGCTATGANCGTCCTAGCACCGCAGCGAATGGGACCCGCAAG970.11759428758471031No Hit
CAGGAAANAGCTATGANNCTTGGTGGAGCCATAGGCCGCATGCACGCGCA970.11759428758471031No Hit
CAGGAAANAGCTATGACCGCCCCTACTCCAAAAGGCGGATGAATGGGGAA960.11638197534156898No Hit
CAGGAAANAGCTATGANNTGGGTTATGGCGTGGGATGCCCTGCAACCCCC950.11516966309842763No Hit
CAGGAAANAGCTATGANCGCAAAACACCTTAACACTCGGCTTCTATTTGC940.11395735085528629No Hit
CAGGAAANAGCTATGANNTGACTTTTGCAAGTCACAGAGAACTGTTGTAT940.11395735085528629No Hit
CAGGAAANAGCTATGANCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA940.11395735085528629No Hit
CAGGAAANAGCTATGANCTCCAGTGGTGGGGCTCAACAAAGGCTCCGTGA930.11274503861214494No Hit
CAGGAAANAGCTATGANNGGGCAGGCCGAGGACATTAGAAATTTGGGACG930.11274503861214494No Hit
CAGGAAANAGCTATGANCTCCTCTTCATTTTCACGGTGCCATTCTGATTC910.11032041412586226No Hit
CAGGAAANAGCTATGANCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT900.10910810188272091No Hit
CAGGAAACAGCTATGACCTCCAGTGGTGGGGCTCAACAAAGGCTCCGTGA880.10668347739643821No Hit
CAGGAAANAGCTATGANNTCCCACTTGCTCTTTGAAGGCAATTTCATTCT880.10668347739643821No Hit
CAGGAAANAGCTATGANNGCTACGGGCAATCACTCTCCTACTCCCAACCA850.1030465406670142No Hit
CAGGAAANAGCTATGACCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA840.10183422842387285No Hit
CAGGAAANAGCTATGANNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG830.10062191618073152No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)