FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113853
Sequences flagged as poor quality0
Sequence length300
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCTGGAAACCCATGCCATGCACTCAACACTACAA34463.0267098802842263No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGCACTCCTTC23112.0298103695115635No Hit
CAGGAAACAGCTATGACCAGGTTGCTGGATTCAGTTTGCTGACCAACTTT19381.7021949355748203No Hit
CAGGAAACAGCTATGACCGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT19341.6986816333342116No Hit
CAGGAAACAGCTATGACCTTGGACAGCACCTTGAATGCCTGGGTTCTAAC16361.4369406164088783No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCTGAGGCAGGAGA15791.386876059480207No Hit
CAGGAAACAGCTATGACCTCCAGCCAGCCAGTCCTTGTGTTGATGGCCTC15541.364917920476404No Hit
CAGGAAACAGCTATGACCAGCGTCTCATTCCATAAAGGCCACCATGAAAC14901.3087050846266677No Hit
CAGGAAACAGCTATGACCACACATACTCCCTTGCTCCAGGGCTGCAGAAC13631.1971577384873477No Hit
CAGGAAACAGCTATGACCAGAGGCACAGGTGGAGAGGGACGAGACCCTGA12841.1277700192353297No Hit
CAGGAAACAGCTATGACCTGCTGGGAGAGGGGGATGAGTAGACAGGGCCA12311.081218764547267No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGGACACTGCCCAC11771.0337891842990523No Hit
CAGGAAACAGCTATGACCACCTGGCAGCCCCAAATCTGCGGGTGACCACG11581.0171009986561619No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC11551.0144660219757053No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCAGCACAGGGAGT10600.9310250937612535No Hit
CAGGAAACAGCTATGACCCAGGGTGACTCCGATCCTGGAGCCAACGGTGC10380.9117019314379069No Hit
CAGGAAACAGCTATGACCTCTACGCCCCTCAGCTGTGTCCTGTCTGCATA10080.8853521646333429No Hit
CAGGAAACAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG9660.8484624911069537No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGGCTGTTT8880.7799530974150879No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGACACTGCAGCCTGGG8660.7606299350917411No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG8610.7562383072909805No Hit
CAGGAAACAGCTATGACCAGGCAGTAACTTGGGCATTTCTTCTGATTCTC8590.7544816561706762No Hit
CAGGAAACAGCTATGACCACCTTTACCCAAATAGCTACCACCAGTGCCTT8450.7421850983285465No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACCCTGTCTCTCTAGC8330.731645191606721No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAGGCACTACGT8310.7298885404864167No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATGGTGAGCCCTGC8190.7193486337645912No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACACAGCTGGGAGGC7700.676310681317137No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCATA7340.6446909611516605No Hit
CAGGAAACAGCTATGACCATCCACTGTTGGCCAACACACCTGGCCTTCAA7220.6341510544298349No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC7070.6209761710275531No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT7040.6183411943470967No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCTCCCCGCCAAGA7000.6148278921064881No Hit
CAGGAAACAGCTATGACCATCGCACCACTGCACTCCAGCGCTCCATACAC6760.5937480786628372No Hit
CAGGAAACAGCTATGACCGATGGCAGAATGGAAACATGGCAGCTGCCTTG6760.5937480786628372No Hit
CAGGAAACAGCTATGACCAGGACTTGCCCACTCTCCCAGGAAGCAGAAGT6720.5902347764222287No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGACACTGCCCACC6660.5849648230613159No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA6550.5753032418996425No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAACCATGTGCCC6440.5656416607379692No Hit
CAGGAAACAGCTATGACCGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC6330.5559800795762957No Hit
CAGGAAACAGCTATGACCGGGGAGACTGGGCCATACGATAGGCTTAATCC6260.5498318006552309No Hit
CAGGAAACAGCTATGACCATCCACTGTTGGCCAACACAGGGGCGATAGTG6130.5384135683732533No Hit
CAGGAAACAGCTATGACCTGCCCAATAGTTCTTGCCTCCAAAATTATCCT6090.5349002661326447No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTCCCCAACCATAG5650.49625394148595114No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGCCCAAGACACTG5640.49537561592579904No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCACAGGAAACAGC5640.49537561592579904No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGGTCCTCCTC5500.48307905808366924No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC5360.47078250024153956No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGGTGGGGACTGGA5320.46726919800093103No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC5300.46551254688062677No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGACACCCAAGACA5280.4637558957603225No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT5080.44618938455727997No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGCAGGAAACAGCT5080.44618938455727997No Hit
CAGGAAACAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT5020.4409194311963673No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA4830.42423124555347685No Hit
CAGGAAACAGCTATGACCACACATACTCCCTTGCTCCACGTGTGCTGTGG4800.4215962688730205No Hit
CAGGAAACAGCTATGACCGGCGGGCCCTGATATAGAGCCACTGGCCGTCG4800.4215962688730205No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAACTGGCCAGCCTG4600.404029757669978No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGCTCAAGG4560.4005164554293695No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACCACCGTGATCTCTC4520.3970031531887609No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG4460.3917331998278482No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTCAGGAATCATGC4440.38997654870754395No Hit
CAGGAAACAGCTATGACCGATGCTCCTTCCTTGGCCCCAGGCCCTGGCGT4270.3750450141849578No Hit
CAGGAAACAGCTATGACCCCCATCCACTAGTCGGGGAGTTGCTTGTCATT4170.3662617585834365No Hit
CAGGAAACAGCTATGACCGATGGCAGAATGGAAACATGGCACCTTGCTTC4040.3548435263014589No Hit
CAGGAAACAGCTATGACCAGAGGCACAGGTGGAGAGGGGGTGATGGCCAT4030.3539652007413068No Hit
CAGGAAACAGCTATGACCCCCGTGACAGCTCCTGCAATTACAAAATGGCT4000.3513302240608504No Hit
CAGGAAACAGCTATGACCCCTGACCCCTTCTTCTCCAGGATGTAAAACTT3950.34693859626008977No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT3840.3372770150984164No Hit
CAGGAAACAGCTATGACCGAGGCGCAGGTCACAGTGAGACCAATACAGAG3830.33639868953826424No Hit
CAGGAAACAGCTATGACCCCAGCAGAGCTCTTCTTACAGAGGCAGGAAAC3760.3302504106171994No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCGTGTGCGCTGGA3750.32937208505704724No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGACACACCACT3650.320588829455526No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTATTGCAGCCTGT3570.31356222497430897No Hit
CAGGAAACAGCTATGACCGATGCTCCTTCCTTGGCCCCGGTACTGGCAGT3510.30829227161339623No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATGGAGCCCTGCAG3510.30829227161339623No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCTGCCAGGAGCAGG3360.29511738821111433No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGTGGGGACTGGAA3350.2942390626509622No Hit
CAGGAAACAGCTATGACCAGTGTGAAGTCTGGATCAAATATTGCCAGGAA3350.2942390626509622No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT3300.2898474348502016No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGATACTACTA3270.2872124581697452No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCCCCGGGCAGA3220.2828208303689846No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACGGGGATGGCAGTG3200.2810641792486803No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGGCTGCTT3190.28018585368852816No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT3190.28018585368852816No Hit
CAGGAAACAGCTATGACCTGCCCAGTTGTTTCCAGAACCTAGCAGAGTCC3160.27755087700807185No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA3140.2757942258877676No Hit
CAGGAAACAGCTATGACCGGGCCTTCTCGAAGCTCTCCATGAGGTCACTG3110.2731592492073112No Hit
CAGGAAACAGCTATGACCCCCATCCACTAGTCGGGGAGTAGGAGGCTGTC3060.2687676214065506No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTGGCATGAAGGA3060.2687676214065506No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCAGAGCCACC3050.2678892958463984No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA3000.2634976680456378No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCCCTCAGCGGG2990.2626193424854857No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGAACAAAAAGCCTCAC2960.25998436580502926No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGTCTGCAGCCTGG2900.2547144124441165No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG2900.2547144124441165No Hit
CAGGAAACAGCTATGACCCTGGGTGCCCTAGACCCAGTATCCAAGGCTCA2890.2538360868839644No Hit
CAGGAAACAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG2870.25207943576366015No Hit
CAGGAAACAGCTATGACCCTGGGCCCCAAAGCAAAGGAGTAGGTGACGAG2810.2468094824027474No Hit
CAGGAAACAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG2740.2406612034816825No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTAGTGGCATGAA2740.2406612034816825No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG2710.23802622680122615No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGTCTGGGTCCT2700.23714790124107402No Hit
CAGGAAACAGCTATGACCACCCAGTTAGAGCCATGGTTCCTGTAGGCTAG2640.23187794788016125No Hit
CAGGAAACAGCTATGACCACCCAGTTAGAGCCATGGTTCCTAGGCTAGCA2610.22924297119970488No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGACAGGAAACAGCT2570.2257296689590964No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGCAGGACACCCCA2520.22133804115833572No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGCCTGGCTTGTC2520.22133804115833572No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTCCAGTCGGTACT2410.21167645999666237No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCTCCGTGTGCGCT2400.21079813443651024No Hit
CAGGAAACAGCTATGACCTGCCCAATAGTTCTTGCCTCCATCTGCCCCTT2390.2099198088763581No Hit
CAGGAAACAGCTATGACCATCGCACCACTGCACTCCAGTGGCGCTCCATA2380.20904148331620598No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC2360.2072848321959017No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGACCGGGATTCGTG2200.1932316232334677No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGCTGGGGACTGGA2160.1897183209928592No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCCGTGTGCGCTGG2150.1888399954327071No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGCCCCGGGAGCTG2150.1888399954327071No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTACTGGCATGAA2090.18357004207179434No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGTCCTGTGCCCA2080.1826917165116422No Hit
CAGGAAACAGCTATGACCTGAAGTGGCTCCATCCTTCCATTTGTTTATAT2050.1800567398311858No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTATGTGACT2050.1800567398311858No Hit
CAGGAAACAGCTATGACCACCTTTACCCAAATAGCTACCACCACTGGCAA2020.17742176315072944No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGCAGGTCGTGCTG2010.17654343759057733No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATCCGAGTGAGGAG1960.1721518097898167No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATCCAGCAGCAGAG1870.16424687974844757No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA1860.16336855418829543No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGGGGGCCTGGCT1800.1580986008273827No Hit
CAGGAAACAGCTATGACCCCAGGAGAGAGCAGCCTTCCTCACACCCGTGA1790.15722027526723056No Hit
CAGGAAACAGCTATGACCAGAGCTACTTCTGTGCCCACCAAACCATTCAG1760.15458529858677417No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC1750.15370697302662203No Hit
CAGGAAACAGCTATGACCCTGGGTGCCGGGTAGAGTGTCAGGAAACAGTC1730.1519503219063178No Hit
CAGGAAACAGCTATGACCCCCATCCACTAGTCGGGGAGTGTCATTGGTGC1710.15019367078601353No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA1710.15019367078601353No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC1660.14580204298525293No Hit
CAGGAAACAGCTATGACCAGAGCTACTTCTGTGCCCACCAAACTATTCAG1650.1449237174251008No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCAGAGAGTGATAT1630.14316706630479653No Hit
CAGGAAACAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC1620.1422887407446444No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCAGCCAGGAGCAGG1620.1422887407446444No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTTGGCATGAAGG1600.14053208962434016No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGAGCTGCGAGCAT1600.14053208962434016No Hit
CAGGAAACAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC1580.13877543850403593No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGACAGGGCTGACTG1560.13701878738373163No Hit
CAGGAAACAGCTATGACCATCGCACCACTGCACTCCAGCCTGGGTGACAG1540.1352621362634274No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGGGAGGGAGCTGC1520.13350548514312316No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT1510.13262715958297103No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAAACCCCATCTCTA1490.13087050846266676No Hit
CAGGAAACAGCTATGACCTCTACGCCCCTCAGCTGTGTCCTATCTGCATA1450.12735720622205826No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT1450.12735720622205826No Hit
CAGGAAACAGCTATGACCATCGCACCACTGCACTCCAGGAAACAGCTATG1430.12560055510175402No Hit
CAGGAAACAGCTATGACCATCGCACCACTGCACTCCAGCCACTGCGCTCC1400.12296557842129763No Hit
CAGGAAACAGCTATGACCATCGCACCACTGCACTCCAGCAGGAAACAGCT1390.12208725286114552No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAACTGGCCGTCGTT1370.12033060174084124No Hit
CAGGAAACAGCTATGACCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG1360.11945227618068914No Hit
CAGGAAACAGCTATGACCATCGCACCACTGCACTCCAGCCTGCGCTCCAT1350.11857395062053701No Hit
CAGGAAACAGCTATGACCAGAGCTACTTCTGTGCCCACCATCCATTCAGC1280.11242567169947214No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGCTGGTGTGGGGA1260.11066902057916786No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC1250.10979069501901575No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC1240.10891236945886362No Hit
CAGGAAACAGCTATGACCAGGACTTGCCCACTCTCCCAGTTACTTGGAAT1220.10715571833855937No Hit
CAGGAAACAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG1210.10627739277840724No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT1200.10539906721825512No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC1180.10364241609795086No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTTTTTCCAAGGGG1170.10276409053779874No Hit
CAGGAAACAGCTATGACCAGAGCTACTTCTGTGCCCACCAGGAAACAGCT1150.1010074394174945No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCCCGTGTGCGCTG1140.10012911385734237No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)