FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246222

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246222
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87131
Sequences flagged as poor quality0
Sequence length300
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCTGGAAACCCATGCCATGCACTCAACACTACAA30843.5394979972684806No Hit
CAGGAAACAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG26513.0425451331902535No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCTGAGGCAGGAGA21442.460662680331914No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGCACTCCTTC19792.2712926512951763No Hit
CAGGAAACAGCTATGACCTTGGACAGCACCTTGAATGCCTGGGTTCTAAC18212.089956502278179No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACCCCGTTAGCTGC12561.4415076149705615No Hit
CAGGAAACAGCTATGACCGCTAATGCTTACTCTTTGCGTGAGTACTGGTT12181.397895123434828No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC11721.3451010547336768No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC11281.2946023803238802No Hit
CAGGAAACAGCTATGACCACCTTTACCCAAATAGCTACCACCAGTGCCTT10801.2395129173313746No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGGCTGTTT10271.1786849686104832No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCAGCTCCGA9791.1235955056179776No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA9621.104084654141465No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCGGGGATCCTCG8690.9973488195934856No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC8670.9950534253021313No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC8360.9594748137861381No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT8340.9571794194947837No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG8310.9537363280577521No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT7930.9101238365220186No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA7720.8860221964627975No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA7480.8584774649665446No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG7260.8332281277616463No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT7070.8114218819937794No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG6970.7999449105370074No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATGGTGAGCCCTGC6910.7930587276629443No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA6600.7574801161469511No Hit
CAGGAAACAGCTATGACCGGCGGGCCCTGATATAGAGCCACTGGCCGTCG6440.739116961816116No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGCTCAAGG6110.7012429560087684No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGCCCGGACGAGGA6060.6955044702803824No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCTGGGTGCTGGTG5920.6794367102409016No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGGTCCTCCTC5860.6725505273668384No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC5470.6277903386854277No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA5370.6163133672286557No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT5340.6128702757916241No Hit
CAGGAAACAGCTATGACCGAGGCGCAGGTCACAGTGAGACCAATACAGAG5120.5876209385867258No Hit
CAGGAAACAGCTATGACCGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA5000.5738485728385994No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTGGCAGCTGCAC4930.565814692818859No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC4840.5554854185077642No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGGCTGCTT4790.5497469327793783No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT4750.5451561441966694No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAACCATGTGCCC4590.5267929898658342No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC4240.48662358976713227No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC4240.48662358976713227No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCTTCCCATCTCA4170.47858970974739184No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT4130.473998921164683No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT4090.4694081325819743No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA4040.4636696468535883No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG3940.4521926753968163No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCAGGAAACAGCT3910.4487495839597847No Hit
CAGGAAACAGCTATGACCTCCAGCCAGCCAGTCCTTGTGTTGATGGCCTC3890.4464541896684303No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT3830.43956800679436714No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA3680.42235254960920915No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTATGACCGCCCACC3680.42235254960920915No Hit
CAGGAAACAGCTATGACCTGGCATCCAGGGCCTATTTGGTTCCTATTGAG3590.41202327529811433No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTCAGGAATCATGC3510.40284169813269677No Hit
CAGGAAACAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG3440.39480781811295634No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGTCTGCAGCCTGG3430.3936601209672792No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT3390.38906933238457037No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG3340.38333084665618433No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA3280.3764446637821212No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA3040.3488999322858684No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC2990.34316144655748243No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGATACTACTA2980.34201374941180523No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCTGCCCAACACCC2960.3397183551204508No Hit
CAGGAAACAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG2920.335127566537742No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC2900.3328321722463876No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT2840.32594598937232444No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCACTGGCCGTCGTT2780.31905980649826127No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGTCTGTGGGCGGA2630.3018443493131033No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGCTCCTCCTCGC2550.29266277214768566No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGCATCCTGG2530.29036737785633127No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA2450.2811858006909137No Hit
CAGGAAACAGCTATGACCTCCCTTAAACTTGGGATTGCTCCATTGCACTC2440.2800381035452365No Hit
CAGGAAACAGCTATGACCGTGCTCCTTGGGGACGTAGTCAGGGGCCACGC2390.27429961781685047No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACCAGGAAACAGCT2390.27429961781685047No Hit
CAGGAAACAGCTATGACCTGAAGTGGCTCCATCCTTCCATTTGTTTATAT2300.2639703435057557No Hit
CAGGAAACAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG2270.2605272520687241No Hit
CAGGAAACAGCTATGACCCCGTAGCCACGCCCCTATTGCAGGAAACAGCT2240.2570841606316925No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT2240.2570841606316925No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCAGGGCAGCGGT2230.2559364634860153No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT2170.24905028061195214No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATGGAGCCCTGCAG2140.2456071891749205No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC2130.24445949202924333No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCAGCTCTGA2100.24101640059221172No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCACAGGAAACAGC2070.23757330915518013No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTATTGCAGCCTGT2060.23642561200950293No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC2060.23642561200950293No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC2010.23068712628111693No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG1980.22724403484408537No Hit
CAGGAAACAGCTATGACCTGCCCAGTTGTTTCCAGAACCTTGTTTTTGAC1930.22150554911569936No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGTCCTGTGCCCA1920.22035785197002217No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATCCGAGTGAGGAG1920.22035785197002217No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGAAAAGTTTCAGG1890.21691476053299058No Hit
CAGGAAACAGCTATGACCGCTTCTCGGCCATGTGGGTTAAACTCGGGGAT1840.21117627480460457No Hit
CAGGAAACAGCTATGACCGTGCTCCTTGGGGACGTAGTTGTTTCCTGCAG1830.21002857765892738No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTAGCCCACCGCAAA1810.20773318336757296No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTGACCCTGGCAG1790.20543778907621857No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAGGCCCCTGTACC1780.20429009193054137No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTCAGGAAACA1770.20314239478486418No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCCAGCTAAGTGA1720.19740390905647817No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGCCTGGCTTGTC1700.19510851476512378No Hit
CAGGAAACAGCTATGACCGTGCTCCTTGGGGACGTAGTTTTACACAGAAC1700.19510851476512378No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCCAGAGAGCACCCG1670.19166542332809217No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCCCACAGAGAGCAC1670.19166542332809217No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCAGAGAGTGATAT1630.18707463474538338No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC1600.1836315433083518No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGACAAGTGCATGG1550.1778930575799658No Hit
CAGGAAACAGCTATGACCTGCTGCTTTTCCTGCCAGAAGACAGAAGCCCA1540.1767453604342886No Hit
CAGGAAACAGCTATGACCTGCTGCTTTTCCTGCCAGAAGCCCAGCCTTCA1480.1698591775602254No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTATGTGACT1430.16412069183183942No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC1430.16412069183183942No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGCCGGGACGCCCC1420.16297299468616222No Hit
CAGGAAACAGCTATGACCAGACAGGGTCTTGCTATGTTGCCCAGGCTAGA1400.16067760039480783No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGCAGGGGCATCCGG1400.16067760039480783No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG1390.15952990324913063No Hit
CAGGAAACAGCTATGACCCCTGACCCCTTCTTCTCCAGGATGTAAAACTT1350.15493911466642182No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAACCAGTGAC1330.1526437203750674No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC1320.15149602322939024No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC1310.150348326083713No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGTCTGGGTCCT1300.14920062893803585No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGGGGGCCTGGCT1300.14920062893803585No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGACACACCACT1290.14805293179235862No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCCTGGGTGTCTG1280.14690523464668145No Hit
CAGGAAACAGCTATGACCGGCAGACAGCCTCTCCTCCTTTCATCACAAAA1270.14575753750100423No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGACCTCTCCTCCT1250.14346214320964984No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTTGGGGTGGATC1240.14231444606397264No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTGATCCCCCAAAA1230.14116674891829545No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCCCGTGTCAAGC1210.13887135462694106No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGTACC1200.13772365748126383No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA1180.13542826318990944No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTCCTGCCTGGTAT1170.13428056604423225No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCCTGGCTTGTCC1110.12739438317016907No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGGCTCTTTT1110.12739438317016907No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCAGATTTTCAAT1080.12395129173313746No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTGTAATCCCAGC1060.12165589744178307No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGCTGTCCTCCTC1050.12050820029610586No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT1050.12050820029610586No Hit
CAGGAAACAGCTATGACCCCGTAGCCACGCCCCTATTGTCTGACCACGCT1050.12050820029610586No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTACTGACCATCT1050.12050820029610586No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGATTCTCAGATTCC1050.12050820029610586No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTCACATCTATAAT1030.11821280600475147No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGCCTGCCTAGGCC1030.11821280600475147No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAATAGTCT1020.11706510885907426No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGGCACGGGAACA1020.11706510885907426No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTTGACTGTAGCC1020.11706510885907426No Hit
CAGGAAACAGCTATGACCTGCTGCTTTTCCTGCCAGAACAGGAAACAGAA980.11247432027636547No Hit
CAGGAAACAGCTATGACCAGACAGGGTCTTGCTATGTTGCCCCAGCTGGA960.11017892598501108No Hit
CAGGAAACAGCTATGACCGGGTCCCACCCATGCTGAAAGTCCACCTTCCA950.10903122883933387No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCTGGGGGCCTGAGA950.10903122883933387No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCACCCCCCCATC950.10903122883933387No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAGTAGTCT940.10788353169365668No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATAAACCAGTTACC930.10673583454797947No Hit
CAGGAAACAGCTATGACCACCTTTACCCAAATAGCTACCACCACTGGCAA920.10558813740230229No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGAGAG920.10558813740230229No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGTGCAGGGAGGCG920.10558813740230229No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGGTACCCGTGTC880.10099734881959348No Hit
CAGGAAACAGCTATGACCACCTTTACCCAAATAGCTACCACCACACAGGA880.10099734881959348No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTGAGGTGGGAGGA880.10099734881959348No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)