FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90842
Sequences flagged as poor quality0
Sequence length300
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC24642.7124017524933404No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC14571.6038836661456155No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGGTTGGAGGTG14451.590673917351005No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC13921.532330860174809No Hit
TGTAAAACGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT13881.5279276105766055No Hit
TGTAAAACGACGGCCAGTTGCCCAACGTTGTAGTGTTGAGTGCATGGCAT12841.4134431210233152No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC12201.3429911274520596No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC11891.3088659430659826No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAGGACGGGTGGCC11861.30556350586733No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG11161.2285066378987692No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC10161.1184253979436825No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC9591.0556790911692828No Hit
TGTAAAACGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT9391.0336628431782655No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA8800.9687149116047643No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCTCCTTCCCAT8530.9389929768168909No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA8490.9345897272186874No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG8480.9334889148191364No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG8250.9081702296294665No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC7760.854230422051474No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGTGAACGAAG7750.8531296096519231No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG7670.8443231104555162No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC7320.8057946764712357No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC6970.7672662424869554No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC6770.745249994495938No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAGGCACGCAGGCT6630.7298386209022258No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC6550.7210321217058189No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCAGCCTGGGTGAT6520.7177296845071662No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCGAGCAGGTGGC6230.6858061249201911No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTGTAAAACGACGG6210.6836045001210894No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC6150.6769996257237841No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCAGGCCCCAAGGA5950.6549833777327668No Hit
TGTAAAACGACGGCCAGTACCGACTGGAGAGGAAGGCACTGGTGGTAGCT5640.6208581933466899No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG5610.6175557561480373No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG5520.6076484445520793No Hit
TGTAAAACGACGGCCAGTGACTGTAGGTTGGGCTCCCCCACAGGATCCTC5500.6054468197529776No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT5270.5801281345633077No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGCCTGGGTGGGA5230.5757248849651042No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGCCCCAATCCTGTCTC5160.5680191981682482No Hit
TGTAAAACGACGGCCAGTAGGAGGGCGGAGGAGGAAAGCAGGTTTGCTCT5110.5625151361704938No Hit
TGTAAAACGACGGCCAGTGGGGTGGGGAGTGAGATGGTCCTAGGAAGTAC5030.5537086369740869No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCAGGTGACTCCAG4990.5493053873758834No Hit
TGTAAAACGACGGCCAGTGAGACAGGCTTCTGGGCTGTCCACAACTCTCT4910.5404988881794764No Hit
TGTAAAACGACGGCCAGTTGTCTGGTGGTGGAAGGAGGTGCACGCCACCA4520.4975672045969926No Hit
TGTAAAACGACGGCCAGTAGATCATCCAGCGCACACGGCGTCTTGGCGGG4380.4821558310032804No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC4350.4788533938046278No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCCGAGTAGCTGAG4250.4678452698091191No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCTGGAGGGCAGCCA4250.4678452698091191No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGGCTTCTGGGCTT4170.45903877061271214No Hit
TGTAAAACGACGGCCAGTTGGATGGTGAGGGAAGGGCTAAGACAGGATTC4110.452433896215407No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT4100.45133308381585613No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCTGGAGGGCGGCCA4100.45133308381585613No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT4050.44582902181810175No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTAGGGTTGGGTCA3970.43702252262169483No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC3950.4348208978225931No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG3870.4260143986261861No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCAGGGCTTGAACCC3840.42271196142753353No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCCGGCCCCAAGGA3830.42161114902798263No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCAGCAGGATGGAG3820.4205103366284318No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC3780.41610708703022825No Hit
TGTAAAACGACGGCCAGTCTTGCACTACATGGCCATCAGGGTCTCGTCCC3730.4106030250324739No Hit
TGTAAAACGACGGCCAGTTGGGGTCTCCTGGTCCCATTCGAGCATGCTGC3730.4106030250324739No Hit
TGTAAAACGACGGCCAGTAGTCAGCCACGTTGGAACCAGTACTCACGCAA3720.40950221263292313No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG3630.39959490103696527No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG3620.3984940886374144No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGCCTTCGGAGGC3590.39519165143876184No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGTGCTGCTGAATG3530.38858677704145655No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG3500.385284339842804No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGTCCAACAATGGCCTG3460.38088109024460054No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGACAGGGTCAGT3350.36877215384954093No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAGGATCCCCGGTG3320.36546971665088834No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCCCCAGTAGCTGG3280.36106646705268486No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT3150.34675590585852356No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCCACGCTGGGAAT3060.33684859426256575No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG3050.3357477818630149No Hit
TGTAAAACGACGGCCAGTGGCTCCCCAGCTGTCTCTCTTGTAAAACGACG3040.33464696946346406No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGTTCGGAGGCAGG2990.32914290746570973No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG2960.3258404702670571No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGAAGCCCAGTCAGG2870.3159331586710993No Hit
TGTAAAACGACGGCCAGTCTGTCTGGCATGGGCCTCTCCACCGTGCCTGA2820.31042909667334495No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGCGCAGCCCTCCTAC2810.30932828427379405No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCCCTCGGAGCTG2810.30932828427379405No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG2780.30602584707514147No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGAAGCCTCAACCC2720.29942097267783624No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGGGCCCCGGGAGC2700.2972193478787345No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATCACATTTCAGCA2670.2939169106800819No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC2640.2906144734814293No Hit
TGTAAAACGACGGCCAGTGACCTCATGGGGCGCTTCCAGCCTCAGGGTCG2630.2895136610818784No Hit
TGTAAAACGACGGCCAGTATTCCTCCACTATCGCCCCTACGTAAAGAAAT2610.2873120362827767No Hit
TGTAAAACGACGGCCAGTCCACTGCATGGCTACTGAATGCTCACCACAAT2580.2840095990841241No Hit
TGTAAAACGACGGCCAGTAGATCATCCAGCGCACACGGAGACAGGCCCAG2570.2829087866845732No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA2490.2741022874881663No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG2420.26639660069131016No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACACGGCAGGAGA2380.26199335109310673No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC2370.26089253869355583No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGAGGCTCCTTCC2320.2553884766958015No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTCACCATTTGGTG2310.25428766429625066No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG2280.250985227097598No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGACGGCACTGGTG2190.2410779155016402No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCAGTGGG2180.23997710310208936No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA2180.23997710310208936No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCCCCCATGAACGG2150.23667466590343672No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCGTGACCGAGAGCT2140.23557385350388588No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCCAAAGTGCTGGG2130.234473041104335No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCCT2120.2333722287047841No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG2080.22896897910658068No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATGGCCGAGCAGGG1990.21906166751062287No Hit
TGTAAAACGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG1860.20475110631646157No Hit
TGTAAAACGACGGCCAGTTTTCCCCTTCTCCCTCCAGGTCAGGGCCCTCC1840.2025494815173598No Hit
TGTAAAACGACGGCCAGTTTTCCCCTTCTCCCTCCAGGCCCCCAAAGGCT1840.2025494815173598No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCAGGAAATCAGGCT1820.20034785671825808No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGGGCGGCGGCTCCT1810.1992470443187072No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGACCTGGCGCAGGC1760.19374298232095286No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTGACCCTTCCAGGA1740.1915413575218511No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTACCCAGTGGTGTG1730.19044054512230027No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG1720.18933973272274937No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACACTGCCGAGTG1700.18713810792364766No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT1680.18493648312454591No Hit
TGTAAAACGACGGCCAGTCGCACAGTCTGTTCTGCAGCCCTGGAGCAAGG1660.18273485832544417No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTATTTCCCTACTT1630.17943242112679156No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC1620.17833160872724071No Hit
TGTAAAACGACGGCCAGTAGATCATCCAGCGCACACGGGAGACAGGCCCA1610.17723079632768984No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCTTGTGACAATGA1590.1750291715285881No Hit
TGTAAAACGACGGCCAGTGCCAATCCTGAGGCCATCAACACAAGGACTGG1560.1717267343299355No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCAGTTCACT1550.17062592193038462No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGCACATGGTT1530.16842429713128287No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCTCCCCAGTCACC1510.16622267233218113No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA1510.16622267233218113No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGGTCCCCGGATGCC1500.1651218599326303No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA1500.1651218599326303No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCCCAACCCCTCAG1500.1651218599326303No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCCGTGGG1480.16292023513352855No Hit
TGTAAAACGACGGCCAGTACACATTCTTGTAAAGCATGGGATTGCTGAAT1450.15961779793487596No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCGCCTCGGCCTCC1440.15851698553532506No Hit
TGTAAAACGACGGCCAGTCTTGCACTACATGGCCATCACCCCCTCTCCAC1440.15851698553532506No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC1420.15631536073622332No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCAGCTCACT1400.15411373593712158No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCCAGGATGGAGGG1380.15191211113801983No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATCGCATTTCAGCA1360.14971048633891812No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCATTTCTCCTTC1340.14750886153981638No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCTGCAGACAGGAT1340.14750886153981638No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCACCTTGGCCTCC1300.14310561194161292No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGTGTTCGGAGGCA1300.14310561194161292No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCCGCCCCAAGGAT1260.13870236234340944No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCAGAAATCAGGCTG1200.13209748794610424No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGGGGCCCGGATGT1190.13099667554655336No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCACCGAGGCAG1180.1298958631470025No Hit
TGTAAAACGACGGCCAGTGCCATCGGCTCCCAAGTTTTATGTCCTAGAGC1180.1298958631470025No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT1170.12879505074745162No Hit
TGTAAAACGACGGCCAGTTCTTTGGAATTTAGTGTCTGAGCCTTGGATAC1130.12439180114924815No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCGGCTCACT1100.12108936395059554No Hit
TGTAAAACGACGGCCAGTCCAGGCATCTCCCAGCTGCGGTCATAGCTGTT1070.11778692675194294No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAGAGCAAAGTC1060.11668611435239205No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGGTCTGGGTG1060.11668611435239205No Hit
TGTAAAACGACGGCCAGTCTGTCTGGCATGGGCCTCTCCACTCTCTGCCG1020.1122828647541886No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAGAGCAAAGTCC1020.1122828647541886No Hit
TGTAAAACGACGGCCAGTCTGTCTGGCATGGGCCTCTCCACTCTCTACCG1020.1122828647541886No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCCGAGTAGCTGGG1000.11008123995508685No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT1000.11008123995508685No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTCCATTTGGTGTG970.10677880275643425No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCTACTTCAATGC970.10677880275643425No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCAGGAAACAACT940.10347636555778164No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC930.10237555315823078No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGAAGGGTGTGTG920.1012747407586799No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)