FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90842
Sequences flagged as poor quality0
Sequence length300
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACCCCGTTAGCTGC15411.6963519077078886No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC15051.656722661324057No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC14691.6170934149402258No Hit
CAGGAAACAGCTATGACCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC13661.5037097377864863No Hit
CAGGAAACAGCTATGACCTGGAAACCCATGCCATGCACTCAACACTACAA13461.481693489795469No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG12791.407939059025561No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA12761.4046366218269082No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA12371.3617049382444244No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCCGTCCTCGGAC11921.3121683802646353No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC11701.2879505074745163No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC11511.2670350718830496No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT10401.1448448955329034No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA10261.129433521939191No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT9211.0138482199863499No Hit
CAGGAAACAGCTATGACCTCCCTTAAACTTGGGATTGCTCCATTGCACTC9191.0116465951872482No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG8840.9731181612029677No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT8640.9511019132119504No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTCCCCAACCATAG8530.9389929768168909No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT8290.9125734792276701No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA7410.8157019880671936No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA7360.8101979260694392No Hit
CAGGAAACAGCTATGACCCCGTAGCCACGCCCCTATTGTCTGACCACGCT7200.7925849276766253No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG7070.7782743664824641No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA6680.7353426828999802No Hit
CAGGAAACAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG6530.7188304969067172No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACGGAGATCCAGCC6450.7100239977103102No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCTGCAGTGCGGT6430.7078223729112085No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCAGAGCCACC6410.7056207481121067No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG6100.6714955637260298No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT5870.6461768785363599No Hit
CAGGAAACAGCTATGACCACCTTTACCCAAATAGCTACCACCAGTGCCTT5850.6439752537372582No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTGTGGGGGAGCC5700.6274630677439951No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGCTCCTCCTCGC5180.5702208229673499No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC5020.552607824574536No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA4960.5460029501772308No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCTGGGTGCTGGTG4860.5349948261817221No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGGAGTCAC4820.5305915765835186No Hit
CAGGAAACAGCTATGACCTGGGCCTGGACACTTTGACGGCGGGGCACGTC4770.5250875145857643No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC4610.5074745161929504No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTAGTCTC4550.5008696417956452No Hit
CAGGAAACAGCTATGACCGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC4480.4931639549987891No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC4330.4766517690055261No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCTCCCCGCCAAGA4320.4755509566059752No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC4270.47004689460822086No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCTCTGCAGCTAGC4110.452433896215407No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGACTGGCCGTCGTT4110.452433896215407No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT4090.45023227141630523No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG4070.4480306466172035No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG4020.44252658461944916No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCTCTTTTAGG3970.43702252262169483No Hit
CAGGAAACAGCTATGACCGCTAATGCTTACTCTTTGCGTGAGTACTGGTT3920.43151846062394045No Hit
CAGGAAACAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG3810.4194095242288809No Hit
CAGGAAACAGCTATGACCGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG3800.41830871182933005No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA3800.41830871182933005No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCCGGAGAGCCTGT3760.41390546223112656No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAAGGCCGCAGCTCC3740.4117038374320248No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTAGTTCC3730.4106030250324739No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT3720.40950221263292313No Hit
CAGGAAACAGCTATGACCGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA3700.4073005878338214No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT3700.4073005878338214No Hit
CAGGAAACAGCTATGACCAGAGGCACAGGTGGAGAGGGACGAGACCCTGA3620.3984940886374144No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA3600.3962924638383126No Hit
CAGGAAACAGCTATGACCGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG3590.39519165143876184No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACCCTGTCTCTCTAGC3560.3918892142401092No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT3550.3907884018405583No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT3540.38968758944100745No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCAGGGCAGCGGT3500.385284339842804No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT3490.3841835274432531No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC3420.37647784064639706No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT3370.3709737786486427No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA3320.36546971665088834No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGACACTGCAGCCTGGG3280.36106646705268486No Hit
CAGGAAACAGCTATGACCTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG3220.3544615926553797No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCGGGGATCCTCG3100.34125184386076923No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAACGCAGCTCCCCA3010.3313445322648114No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACCAGGAAACAGCT2980.32804209506615883No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGGCACGGGAACA2950.32473965786750625No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG2910.32033640826930276No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCAAGCCTGACTG2830.3115299090728958No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGCCCGGACGAGGA2810.30932828427379405No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG2790.3071266594746923No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCAGCTCCGA2740.301622597476938No Hit
CAGGAAACAGCTATGACCAAACCCTCGACCCTGAGGCTGGAAGCGCCCCA2730.30052178507738714No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCTTCCCATCTCA2660.292816098280531No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGATTCTCAGATTCC2600.28621122388322584No Hit
CAGGAAACAGCTATGACCGGGTCCCACCCATGCTGAAATGTGATCCTCAA2520.27740472468681887No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC2520.27740472468681887No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT2460.27079985028951364No Hit
CAGGAAACAGCTATGACCATCCACTGTTGGCCAACACACCTGGCCTTCAA2460.27079985028951364No Hit
CAGGAAACAGCTATGACCTCTGACTGTCCGTGATCCCACACAATCACCCT2460.27079985028951364No Hit
CAGGAAACAGCTATGACCGCCCCTACTCCAAAAGGCGGATGAATGGGGAA2450.2696990378899628No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCGTGTTCTCACTC2440.2685982254904119No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACCCTTTGTCCA2410.2652957882917593No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCGTGTGCGCTGGA2380.26199335109310673No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCCTCTCCCAACT2340.25759010149490325No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC2330.25648928909535235No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATGGTGAGCCCTGC2230.2454811650998437No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCACGGGCATCCTG2200.24217872790119108No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCCATGCTCACCT2180.23997710310208936No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT2080.22896897910658068No Hit
CAGGAAACAGCTATGACCGTGCTCCTTGGGGACGTAGTCGCCTGGGGACT2050.22566654190792806No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCCTGCCCCCTGCA1950.21465841791241938No Hit
CAGGAAACAGCTATGACCACTCCCTTGAGCCTTTGGGGAAAGGGAGACAG1900.20915435591466502No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGCGGAAGAGGAGCC1870.2058519187160124No Hit
CAGGAAACAGCTATGACCGGGTCCCACCCATGCTGAAAGTCCACCTTCCA1840.2025494815173598No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGCCTGCCCAGGCC1810.1992470443187072No Hit
CAGGAAACAGCTATGACCACTCCCTTGAGCCTTTGGGGGCCTGGAGGGAG1800.1981462319191563No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGACACACCACT1780.1959446071200546No Hit
CAGGAAACAGCTATGACCGGCGGGCCCTGATATAGAGCCACTGGCCGTCG1780.1959446071200546No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTTATCCC1760.19374298232095286No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCCTGGGTGTCTG1760.19374298232095286No Hit
CAGGAAACAGCTATGACCTCCAGCCAGCCAGTCCTTGTGTTGATGGCCTC1710.18823892032319853No Hit
CAGGAAACAGCTATGACCAGACAGGGTCTTGCTATGTTGCCCAGGCTGGT1680.18493648312454591No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGTCTGGGTCCT1650.1816340459258933No Hit
CAGGAAACAGCTATGACCGGGCAGGCCGAGGACATTAGAAATTTGGGACG1650.1816340459258933No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCACTCAGCGTGTG1600.17612998392813897No Hit
CAGGAAACAGCTATGACCACACATACTCCCTTGCTCCAGGGCTGCAGAAC1560.1717267343299355No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCCGTGTGCGCTGG1530.16842429713128287No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC1500.1651218599326303No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAACCATGTGCCC1490.16402104753307942No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGCAGGGGCATCCGG1480.16292023513352855No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTAGTCCC1450.15961779793487596No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTGCAGTGAACTGA1440.15851698553532506No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGTCTGCAGCCTGG1410.15521454833667248No Hit
CAGGAAACAGCTATGACCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG1410.15521454833667248No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTCACACCTGTAA1390.15301292353757073No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCCAGCTAAGTGA1370.150811298738469No Hit
CAGGAAACAGCTATGACCAGAGGCACAGGTGGAGAGGGGGTGATGGCCAT1350.14860967393936725No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTGGGGAGGGTGG1350.14860967393936725No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCAGGCAAACTTGG1350.14860967393936725No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGCCTGGCTTGTC1340.14750886153981638No Hit
CAGGAAACAGCTATGACCGGGTCCCACCCATGCTGAAATGCGATCCTCAA1340.14750886153981638No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA1330.1464080491402655No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAATGAAACTTGGTT1320.14530723674071466No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAACACGCAGCTCCC1320.14530723674071466No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGTTCTCCTTCCCT1290.14200479954206205No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT1250.13760154994385856No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTGTAATCCCAGCT1240.1365007375443077No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTCCATTCT1230.13539992514475682No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCGGAGAGCCTGTG1220.13429911274520595No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAGTAGTCT1200.13209748794610424No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCTGGGTGTCTGC1190.13099667554655336No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTCCCTTTGGGC1150.12659342594834988No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAATAGTCT1130.12439180114924815No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTCCCTTTGGGCT1040.11448448955329034No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTTGTAATCCCATC1030.11338367715373945No Hit
CAGGAAACAGCTATGACCGTGCTCCTTGGGGACGTAGTTGTTTCCTGCAG1020.1122828647541886No Hit
CAGGAAACAGCTATGACCCTGGGTGCCCTAGACCCAGTATCCAAGGCTCA1020.1122828647541886No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGACCTCTCCTCCT1000.11008123995508685No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATGGAGCCCTGCAG1000.11008123995508685No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTCACGCCTGTAA980.10787961515598511No Hit
CAGGAAACAGCTATGACCGCCCCTACTCCAAAAGGCGGCAGAGAGTGGAG980.10787961515598511No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCCCCTGCCTACC940.10347636555778164No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCAGGCAC930.10237555315823078No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTACTCCCAACCA930.10237555315823078No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)