FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105468
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT12611.1956233170250692No Hit
CAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG12271.1633860507452498No Hit
CAGGAAANAGCTATGANNGGGGAGACTGGGCCATACGATAGGCTTAATCC9200.8723024993362916No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA9160.8685098797739599No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT8970.8504949368528842No Hit
CAGGAAANAGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT8090.7670573064815868No Hit
CAGGAAANAGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT7960.7547312929040089No Hit
CAGGAAANAGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA7760.7357681950923503No Hit
CAGGAAANAGCTATGANNGCTTCTATATGAGCGCATCTCACCTGAAGTTG7610.7215458717336064No Hit
CAGGAAANAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT7190.6817233663291236No Hit
CAGGAAANAGCTATGANNGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT7120.6750862820950431No Hit
CAGGAAANAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG6950.6589676489551334No Hit
CAGGAAANAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT6450.6115599044259871No Hit
CAGGAAANAGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG5860.5556187658815944No Hit
CAGGAAANAGCTATGACCGGGGAGACTGGGCCATACGATAGGCTTAATCC5460.5176925702582774No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGACTGGCCGTCG5410.5129517958053628No Hit
CAGGAAANAGCTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC5300.5025220920089506No Hit
CAGGAAANAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA5290.5015739371183676No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG5180.49114423332195545No Hit
CAGGAAANAGCTATGACCGGGACCAGTCCAATAACCCCTGAGGGGTATAT4510.4276178556528994No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT4400.4171881518564873No Hit
CAGGAAANAGCTATGACCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT4400.4171881518564873No Hit
CAGGAAANAGCTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG4390.41623999696590436No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG4370.41434368718473846No Hit
CAGGAAANAGCTATGACCGCTTCTATATGAGCGCATCTCACCTGAAGTTG4310.40865475784124095No Hit
CAGGAAANAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT4210.39917320893541164No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT4110.38969166002958244No Hit
CAGGAAANAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT4030.38210642090491903No Hit
CAGGAAANAGCTATGANCTGTTTTGCCCCTGAAATGAATATTCATCGTCT4030.38210642090491903No Hit
CAGGAAANAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA3850.36503963287442637No Hit
CAGGAAANAGCTATGANNGTTTTCACTGGCTCCCGGGGCTGTTCACTGGC3790.3593507035309288No Hit
CAGGAAACAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG3750.3555580839685971No Hit
CAGGAAANAGCTATGACCCTGTGAGGCACCAGAGCACCGACTGGCCGTCG3570.33849129593810445No Hit
CAGGAAANAGCTATGANCAGGCACCAACAGTACAAAGCAGATACAAAGCA3520.3337505214851898No Hit
CAGGAAANAGCTATGANCGGGGAGACTGGGCCATACGATAGGCTTAATCC3420.3242689725793606No Hit
CAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA3340.3166837334546972No Hit
CAGGAAANAGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG3270.3100466492206167No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA3250.3081503394394508No Hit
CAGGAAACAGCTATGACCGGGGAGACTGGGCCATACGATAGGCTTAATCC3200.3034095649865362No Hit
CAGGAAANAGCTATGANNAACCAACACTGCTGCTAATTGTGTTTGTGTCC3170.3005651003147874No Hit
CAGGAAANAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG3170.3005651003147874No Hit
CAGGAAANAGCTATGANNCTGGCCTCCTTCAATGCATTTAATGGCCTTAT3140.29772063564303863No Hit
CAGGAAANAGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT3130.2967724807524557No Hit
CAGGAAANAGCTATGACCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC3120.2958243258618728No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT3080.2920317062995411No Hit
CAGGAAANAGCTATGANNACCAAGACCCCAGCAGCAGAGGGAAGGTGACT3010.2853946220654606No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA2910.2759130731596314No Hit
CAGGAAANAGCTATGANCGCTTCTATATGAGCGCATCTCACCTGAAGTTG2870.27212045359729964No Hit
CAGGAAANAGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC2860.2711722987067167No Hit
CAGGAAANAGCTATGANCGGGACCAGTCCAATAACCCCTGAGGGGTATAT2830.2683278340349679No Hit
CAGGAAACAGCTATGACCGGGACCAGTCCAATAACCCCTGAGGGGTATAT2820.267379679144385No Hit
CAGGAAANAGCTATGANNTGACAGCACAAGGAACACTGACCTACAGATAA2790.2645352144726362No Hit
CAGGAAANAGCTATGACCGTTTTCACTGGCTCCCGGGGCTGTTCACTGGC2750.26074259491030455No Hit
CAGGAAANAGCTATGANNTCCTCTTCATTTTCACGGTGCCATTCCGATTC2740.25979444001972163No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT2730.2588462851291387No Hit
CAGGAAANAGCTATGANNCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG2730.2588462851291387No Hit
CAGGAAANAGCTATGANCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT2700.25600182045738995No Hit
CAGGAAANAGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC2680.25410551067622406No Hit
CAGGAAACAGCTATGACCGCTTCTATATGAGCGCATCTCACCTGAAGTTG2680.25410551067622406No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGACTGGCCGTCG2650.2512610460044753No Hit
CAGGAAANAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG2620.24841658133272654No Hit
CAGGAAANAGCTATGANNCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC2610.24746842644214356No Hit
CAGGAAANAGCTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA2610.24746842644214356No Hit
CAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2550.24177949709864605No Hit
CAGGAAANAGCTATGANNGTTTTCACTTGCTTCCGGGGCTGTTCACTGGC2540.2408313422080631No Hit
CAGGAAANAGCTATGANNGCAGCAAGAGACCCAGACCCACCCGACTGGCC2540.2408313422080631No Hit
CAGGAAANAGCTATGANCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT2510.23798687753631434No Hit
CAGGAAANAGCTATGANCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA2490.2360905677551485No Hit
CAGGAAANAGCTATGANNGGTAGCAACGTCCCACCCAGGGCACGGGAACA2460.2332461030833997No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA2440.23134979330223385No Hit
CAGGAAANAGCTATGANNCTGGCCTCCTTCAATGCATTTAATGGCCTTCT2420.229453483521068No Hit
CAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2400.22755717373990214No Hit
CAGGAAANAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2270.21523116016232413No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA2250.21333485038115824No Hit
CAGGAAANAGCTATGANNGCTGCTGACCCAACCCTACCCTGTCCAGCCCC2150.203853301475329No Hit
CAGGAAANAGCTATGANCCTGTGAGGCACCAGAGCACCGACTGGCCGTCG2140.2029051465847461No Hit
CAGGAAANAGCTATGANNTGCTCTGGGCTCTGGATACTGGCCGTCGTTTT2070.1962680623506656No Hit
CAGGAAANAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG2020.19152728789775098No Hit
CAGGAAANAGCTATGANCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC2010.19057913300716806No Hit
CAGGAAANAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1920.18204573899192172No Hit
CAGGAAANAGCTATGANCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG1890.17920127432017294No Hit
CAGGAAACAGCTATGACCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC1850.17540865475784126No Hit
CAGGAAANAGCTATGACCAACCAACACTGCTGCTAATTGTGTTTGTGTCC1850.17540865475784126No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG1820.17256419008609247No Hit
CAGGAAANAGCTATGANNTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC1810.17161603519550955No Hit
CAGGAAANAGCTATGACCGTTTTCACTTGCTTCCGGGGCTGTTCACTGGC1790.1697197254143437No Hit
CAGGAAANAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC1780.16877157052376077No Hit
CAGGAAANAGCTATGANNTCCCACTTGCTCTTTGAAGGCAATTCCATTCT1750.16592710585201198No Hit
CAGGAAANAGCTATGACCCTGGCCTCCTTCAATGCATTTAATGGCCTTAT1750.16592710585201198No Hit
CAGGAAANAGCTATGANCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG1710.1621344862896803No Hit
CAGGAAANAGCTATGACCACCAAGACCCCAGCAGCAGAGGGAAGGTGACT1700.16118633139909735No Hit
CAGGAAANAGCTATGANNTCCTCTTCATTTTCACGGTGCCATTCTGATTC1690.16023817650851443No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGCTGTTCACTGGC1670.1583418667273486No Hit
CAGGAAANAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT1660.15739371183676565No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG1660.15739371183676565No Hit
CAGGAAANAGCTATGACCGCAGCAAGAGACCCAGACCCACCCGACTGGCC1640.1554974020555998No Hit
CAGGAAANAGCTATGANNTGCCAAAATTAAGGATGCACACCTGTGACACA1630.15454924716501686No Hit
CAGGAAANAGCTATGANCGTTTTCACTGGCTCCCGGGGCTGTTCACTGGC1620.15360109227443394No Hit
CAGGAAANAGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG1600.1517047824932681No Hit
CAGGAAANAGCTATGANNGGCTGGCACAAAGAACAGCACTGACTGTTTAT1580.14980847271210224No Hit
CAGGAAANAGCTATGANNGCAGCAAGAGACCAATACCGCATTGTAGTCAA1580.14980847271210224No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1580.14980847271210224No Hit
CAGGAAANAGCTATGANNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT1560.1479121629309364No Hit
CAGGAAANAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1550.14696400804035348No Hit
CAGGAAANAGCTATGACCCTGGCCTCCTTCAATGCATTTAATGGCCTTCT1540.14601585314977056No Hit
CAGGAAANAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC1510.14317138847802177No Hit
CAGGAAANAGCTATGACCTGACAGCACAAGGAACACTGACCTACAGATAA1500.14222323358743885No Hit
CAGGAAANAGCTATGACCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA1480.14032692380627299No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1480.14032692380627299No Hit
CAGGAAANAGCTATGANNGCTTCTATATGAGCGCATCTCACCTGAATTTG1460.13843061402510715No Hit
CAGGAAANAGCTATGANCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1460.13843061402510715No Hit
CAGGAAANAGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1450.1374824591345242No Hit
CAGGAAANAGCTATGACCTGCTCTGGGCTCTGGATACTGGCCGTCGTTTT1450.1374824591345242No Hit
CAGGAAANAGCTATGACCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA1440.13653430424394128No Hit
CAGGAAANAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1420.13463799446277544No Hit
CAGGAAANAGCTATGACCCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG1390.13179352979102665No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1360.1289490651192779No Hit
CAGGAAANAGCTATGACCGGTAGCAACGTCCCACCCAGGGCACGGGAACA1350.12800091022869498No Hit
CAGGAAANAGCTATGACCTCCTCTTCATTTTCACGGTGCCATTCCGATTC1340.12705275533811203No Hit
CAGGAAANAGCTATGANNACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG1330.1261046004475291No Hit
CAGGAAANAGCTATGANCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1310.12420829066636327No Hit
CAGGAAANAGCTATGANNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT1300.12326013577578032No Hit
CAGGAAACAGCTATGACCTCCTCTTCATTTTCACGGTGCCATTCCGATTC1300.12326013577578032No Hit
CAGGAAANAGCTATGACCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC1250.11851936132286571No Hit
CAGGAAANAGCTATGANCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG1240.11757120643228279No Hit
CAGGAAACAGCTATGACCAACCAACACTGCTGCTAATTGTGTTTGTGTCC1230.11662305154169984No Hit
CAGGAAANAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC1210.114726741760534No Hit
CAGGAAANAGCTATGANCAACCAACACTGCTGCTAATTGTGTTTGTGTCC1170.11093412219820231No Hit
CAGGAAANAGCTATGANNGCCCTGGCCACTGTGGTTAAACCCCGTCTCTA1150.10903781241703644No Hit
CAGGAAANAGCTATGANNAAGTTGGTCAGCCCTGGTGACTCATTGGATAT1140.10808965752645353No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC1140.10808965752645353No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG1140.10808965752645353No Hit
CAGGAAANAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1130.1071415026358706No Hit
CAGGAAANAGCTATGANNGCAGCCACCCTGAACACACTTGGCTATCACCC1120.10619334774528766No Hit
CAGGAAANAGCTATGANCCAGGGCTCCAAGAATGTTCCACACATCTTTCT1120.10619334774528766No Hit
CAGGAAACAGCTATGACCGCAGCAAGAGACCCAGACCCACCCGACTGGCC1110.10524519285470474No Hit
CAGGAAANAGCTATGANNGCTTCTATATGAGCGCATCTCACCAGAGCACC1100.10429703796412182No Hit
CAGGAAANAGCTATGANCCTGGCCTCCTTCAATGCATTTAATGGCCTTAT1090.10334888307353889No Hit
CAGGAAANAGCTATGANCTGACAGCACAAGGAACACTGACCTACAGATAA1080.10240072818295597No Hit
CAGGAAANAGCTATGANNTGCCAAAATTAAGGATGCACACCTGCCTCACT1080.10240072818295597No Hit
CAGGAAANAGCTATGANNTGTTGTCTTTCAGGGGCCGCCATGCCTGCCGT1070.10145257329237305No Hit
CAGGAAANAGCTATGANNAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT1060.10050441840179013No Hit
CAGGAAANAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTCCATTCT1060.10050441840179013No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1060.10050441840179013No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)