FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125269
Sequences flagged as poor quality0
Sequence length300
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGCACTCCTTC22271.7777742298573471No Hit
CAGGAAACAGCTATGACCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC20041.5997573222425339No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG19291.5398861649729783No Hit
CAGGAAACAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAACT18241.4560665447955998No Hit
CAGGAAACAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAATT18101.4448905954386162No Hit
CAGGAAACAGCTATGACCTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT17881.427328389306213No Hit
CAGGAAACAGCTATGACCGGGCATGGTGTGGGACTAGCTAATCTAGGGAC17761.417749004143084No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA17751.4169507220461568No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC17371.386616002362915No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC17251.377036617199786No Hit
CAGGAAACAGCTATGACCCAGGGTGACTCCGATCCTGGAGCCAACGGTGC16001.2772513550838596No Hit
CAGGAAACAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT15971.2748565087930774No Hit
CAGGAAACAGCTATGACCTCCACAAGGCCCTGGGACAAGGAAAATGGAGA15381.2277578650743601No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT15081.2038094021665375No Hit
CAGGAAACAGCTATGACCACACATACTCCCTTGCTCCAGGGCTGCAGAAC15021.1990197095849733No Hit
CAGGAAACAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT14211.1343588597338528No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG14121.127174320861506No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTCCCCAACCATAG13671.0912516264997725No Hit
CAGGAAACAGCTATGACCGCTTCTATATGAGCGCATCTCACCTGAATTTG13551.0816722413366435No Hit
CAGGAAACAGCTATGACCTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT13471.0752859845612242No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGGACACTGCCCAC13191.0529340858472567No Hit
CAGGAAACAGCTATGACCAGAGGCACAGGTGGAGAGGGACGAGACCCTGA10760.8589515362938955No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCTGCCCAACACCC10530.8405910480645651No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCAGAGCCACC10490.8373979196768555No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG10340.8254236882229442No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA10170.8118528925751782No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTGTGGGGGAGCC10080.8046683537028315No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT10020.7998786611212669No Hit
CAGGAAACAGCTATGACCGTCCTAGCACCGCAGCGAATGGGACCCGCAAG9500.7583679920810416No Hit
CAGGAAACAGCTATGACCAACCAACACTGCTGCTAATTGTGTTTGTGTCC9390.74958688901484No Hit
CAGGAAACAGCTATGACCAGGTTGCTGGATTCAGTTTGCTGACCAACTTT9120.7280332723977999No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGACTGGCCGTCGTT8730.6969002706176308No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG8470.6761449360975181No Hit
CAGGAAACAGCTATGACCCTTGGTGGAGCCATAGGCCGTCGTTTTACATG8310.6633724225466795No Hit
CAGGAAACAGCTATGACCTCCCACCACTGACTCCAGGGGAAGGAGGAGGA8250.6585827299651151No Hit
CAGGAAACAGCTATGACCACCTGGCAGCCCCAAATCTGCGGGTGACCACG8200.654591319480478No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACACAGCTGGGAGGC8170.6521964731896958No Hit
CAGGAAACAGCTATGACCATCCACTGTTGGCCAACACACCTGGCCTTCAA8150.650599908995841No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC7800.6226600356033815No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGACACTGCCCACC7680.6130806504402526No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG7480.5971150085017043No Hit
CAGGAAACAGCTATGACCAAACCCTCGACCCTGAGGCTGGAAGCGCCCCA7390.5899304696293576No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGCAGGAAACAGCT7310.5835442128539383No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCCGGAGAGCCTGT6830.5452266722014225No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCTCCCCGCCAAGA6780.5412352617167855No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGACACCCAAGACA6380.5093039778396891No Hit
CAGGAAACAGCTATGACCTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG6110.4877503612226488No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATGGTGAGCCCTGC6090.486153797028794No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCAAGCCTGACTG6030.48136410444722955No Hit
CAGGAAACAGCTATGACCCCCTGCGGCTCCTTTCAAGTGCAGAGGAAGCC6020.48056582235030215No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACGGGGATGGCAGTG5870.468591590896391No Hit
CAGGAAACAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG5730.45741564153940717No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGGTCCTCCTC5700.45502079524862493No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCAGGAAACAGCT5680.45342423105477014No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGCGGCCTTGCTGA5570.4446431279885686No Hit
CAGGAAACAGCTATGACCTCCAGCCAGCCAGTCCTTGTGTTGATGGCCTC5390.43027405024387516No Hit
CAGGAAACAGCTATGACCGCATCCTGCTTAGTGCTGCCCACAGTGTCATT5360.427879203953093No Hit
CAGGAAACAGCTATGACCTCCTCTTCATTTTCACGGTGCCATTCTGATTC5320.4246860755653833No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA5280.42149294717767366No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA5150.4111152799176173No Hit
CAGGAAACAGCTATGACCGCTAATGCTTACTCTTTGCGTGAGTACTGGTT5110.40792215152990763No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCAGCTCCGA4930.3935530737852142No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA4670.3727977392651015No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAACAGCAGACACC4660.37199945716817406No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGCCCGGACGAGGA4660.37199945716817406No Hit
CAGGAAACAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG4660.37199945716817406No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG4630.3696046108773918No Hit
CAGGAAACAGCTATGACCTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG4520.3608235078111903No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGACACTGCAGCCTGGG4320.3448578658726421No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG4320.3448578658726421No Hit
CAGGAAACAGCTATGACCACACATACTCCCTTGCTCCACGTGTGCTGTGG4230.3376733270002954No Hit
CAGGAAACAGCTATGACCAGGGCAAGTGTTCGCGTTGTAGGCGGCGGGAC4200.33527848070951316No Hit
CAGGAAACAGCTATGACCCTTGGTGGAGCCATAGGCCGTTTCTGTTCCAT4130.32969050603102124No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT4130.32969050603102124No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCTTCCCATCTCA4110.32809394183716645No Hit
CAGGAAACAGCTATGACCGGCGGGCCCTGATATAGAGCCACTGGCCGTCG3840.3065403252201263No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGCCCAAGACACTG3650.2913729653785055No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCTCCGTGTGCGCT3600.2873815548938684No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG3410.2722141950522476No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGCAGGGGCATCCGG3340.26662622037375566No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGATTCTCAGATTCC3330.26582793827682827No Hit
CAGGAAACAGCTATGACCGGGCAGGCCGAGGACATTAGAAATTTGGGACG3310.2642313740829734No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGACATGCATT3250.25944168150140895No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCGTGTGCGCTGGA3240.25864339940448156No Hit
CAGGAAACAGCTATGACCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG3200.2554502710167719No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGACACACCACT3170.25305542472598963No Hit
CAGGAAACAGCTATGACCACCTGGCAGCCCCAAATCTGCACCTCCCGCAG3140.25066057843520745No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGCGGAAGAGGAGCC3120.2490640142413526No Hit
CAGGAAACAGCTATGACCAAACTATTAATACCTGCAGCAAAGCCCCAAGC3110.24826573214442518No Hit
CAGGAAACAGCTATGACCACCTGGCAGCCCCAAATCTGCACCTCCCGCGG3070.24507260375671555No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGATACTACTA3050.24347603956286074No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGTCTGGGTCCT2990.23868634698129623No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGCACAGACCCCACA2910.23230009020587697No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAGGCCCCTGTACC2910.23230009020587697No Hit
CAGGAAACAGCTATGACCTGACTGCAAACATAGAAGTGAGCAGAGCAAGA2690.21473788407347388No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGTTGAGCTCCTCA2660.21234303778269165No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAGGGGACATGGCC2660.21234303778269165No Hit
CAGGAAACAGCTATGACCAGGGCAAGTGTTCGCGTTGTGACAGTTACGGT2500.19957052423185306No Hit
CAGGAAACAGCTATGACCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG2440.1947808316502886No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCCGTGTGCGCTGG2380.1899911390687241No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCGGAGAGCCTGTG2370.1891928569717967No Hit
CAGGAAACAGCTATGACCGGTCCCGCGGGGAAAGTAAGTCCTTCCCTTGG2360.18839457487486927No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCAGGCAC2360.18839457487486927No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATGGAGCCCTGCAG2340.18679801068101445No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAACACACACACAC2260.18041175390559516No Hit
CAGGAAACAGCTATGACCACTCCCTTGAGCCTTTGGGGGCATTAGCTCCC2240.17881518971174035No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA2220.1772186255178855No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGCAGGACACCCCA2220.1772186255178855No Hit
CAGGAAACAGCTATGACCCACCGCTGTCCCTTCCCAAGGACTATGCTGAA2210.1764203434209581No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAGTAGTCT2200.1756220613240307No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGTCTGCAGCCTGG2140.1708323687424662No Hit
CAGGAAACAGCTATGACCCCCTGCGGCTCCTTTCAAGTTAAGTCACCACT2120.1692358045486114No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCTGGGAAAAAGGG2090.16684095825782913No Hit
CAGGAAACAGCTATGACCCTTGGTGGAGCCATAGGCCGCATGCACGCGCA2040.16284954777319208No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCCCTCGGGGAG1930.15406844470699055No Hit
CAGGAAACAGCTATGACCTCCACAAGGCCCTGGGACAATCTCCCAGGCCC1890.15087531631928092No Hit
CAGGAAACAGCTATGACCCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA1870.14927875212542607No Hit
CAGGAAACAGCTATGACCACTCCCTTGAGCCTTTGGGGGCCTGGAGGGAG1860.14848047002849865No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGCAGGAAACAGCT1830.14608562373771644No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGTCTAGCAGGCAC1800.1436907774469342No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACGGCCCCTCTGCC1770.14129593115615194No Hit
CAGGAAACAGCTATGACCCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG1760.14049764905922454No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGTCTGTGGGCGGA1720.13730452067151488No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGGGGGCCTGGCT1690.13490967438073267No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT1650.13171654599302302No Hit
CAGGAAACAGCTATGACCACTCCCTTGAGCCTTTGGGGACCTCGACCAGA1650.13171654599302302No Hit
CAGGAAACAGCTATGACCAGAGGCACAGGTGGAGAGGGGGTGATGGCCAT1630.13011998179916817No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCTGGGCAGAAGGG1620.12932169970224078No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAATAGTCT1620.12932169970224078No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCAGATTTTCAAT1610.12852341760531336No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGCCTGGCTTGTC1610.12852341760531336No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAACAGTGAAGACAG1580.12612857131453112No Hit
CAGGAAACAGCTATGACCATCCACTGTTGGCCAACACAGGGGCGATAGTG1570.12533028921760372No Hit
CAGGAAACAGCTATGACCTGCTTTGGGGCCATGGAGTTGAGCTGTTGAGA1550.1237337250237489No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGTCCTCAGCCACC1550.1237337250237489No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGAAGGGCCACCCCA1530.12213716082989406No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATCCGAGTGAGGAG1440.11495262195754735No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCACTCAGCGTGTG1430.11415433986061996No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCTGAGAGCCTGTG1430.11415433986061996No Hit
CAGGAAACAGCTATGACCAAACCCTCGACCCTGAGGCTTGGGAAGCGCCC1420.11335605776369254No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCCCGTGTCAAGC1360.10856636518212807No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGCGGCGCTCCCTG1300.10377667260056359No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCCCCTGCCTACC1290.10297839050363616No Hit
CAGGAAACAGCTATGACCCTTGGTGGAGCCATAGGCCGTCGTTTTACAAT1280.10218010840670877No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)