FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003246250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194114
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC40942.1090699279804648No Hit
CAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC33901.7463964474484066No Hit
CAGGAAANAGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT26941.3878442564678488No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT26181.3486920057285925No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG24901.2827513729045819No Hit
CAGGAAANAGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA23691.2204168684381342No Hit
CAGGAAANAGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA20411.051443996826607No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA20351.0483530296629815No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA18590.9576846595299668No Hit
CAGGAAANAGCTATGANNGTCCTAGCACCGCAGCGAATGGGACCCGCAAG18340.9448056296815274No Hit
CAGGAAANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC16840.8675314505908898No Hit
CAGGAAANAGCTATGANNAGAGACCAGATGCATAAGCACAGCAGCATTCC15130.7794388864275632No Hit
CAGGAAANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC14650.7547111491185592No Hit
CAGGAAANAGCTATGANNACACCCGGAGCAGTGCAAATCAACTCCCCCAG14120.7274076058398673No Hit
CAGGAAANAGCTATGANNTAGAGGACACCCAAGGCCACCAGGGCAGCGGT13580.6995889013672378No Hit
CAGGAAANAGCTATGANNCCGTAGCCACGCCCCTATTGTCTGACCACGCT12760.657345683464356No Hit
CAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT12660.6521940715249802No Hit
CAGGAAANNGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT12250.6310724625735392No Hit
CAGGAAANAGCTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA11720.6037689192948473No Hit
CAGGAAANAGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT10780.5553437670647146No Hit
CAGGAAANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA10440.5378282864708367No Hit
CAGGAAANAGCTATGANNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT10310.5311311909496481No Hit
CAGGAAANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG9820.5058882924467066No Hit
CAGGAAANAGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC9080.46776636409532546No Hit
CAGGAAANAGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG8710.4487053999196349No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC8560.4409779820105711No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCCAAACCAG8410.43325056410150736No Hit
CAGGAAANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA8230.4239776626106309No Hit
CAGGAAANAGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC8050.4147047611197544No Hit
CAGGAAANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA7740.39873476410768927No Hit
CAGGAAANAGCTATGANNGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC7390.3807041223198739No Hit
CAGGAAANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA7320.3770979939623108No Hit
CAGGAAANAGCTATGANNAGCGTCTCATTCCATAAAGGCCACCATGAAAC7230.3724615432168725No Hit
CAGGAAANNGCTATGANNGTCCTAGCACCGCAGCGAATGGGACCCGCAAG6880.35443090142905714No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA6830.3518550954593692No Hit
CAGGAAANAGCTATGANNGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG6820.35133993426543164No Hit
CAGGAAANAGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG6430.3312486477018659No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG6270.3230060685988646No Hit
CAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA6000.3090967163625499No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG5860.30188445964742366No Hit
CAGGAAANAGCTATGANNTGCTGGGAGAGGGGGATGAGCGGCGCCGCCAT5830.30033897606561094No Hit
CAGGAAANNGCTATGANNAGAGACCAGATGCATAAGCACAGCAGCATTCC5530.28488414024748343No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG5500.2833386566656707No Hit
CAGGAAANNGCTATGANNACACCCGGAGCAGTGCAAATCAACTCCCCCAG5370.2766415611444821No Hit
CAGGAAANAGCTATGANNTGGGCCTGGACACTTTGACGGCGGGGCACGTC5090.2622170477142298No Hit
CAGGAAANNGCTATGANNTAGAGGACACCCAAGGCCACCAGGGCAGCGGT5060.26067156413241704No Hit
CAGGAAANAGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT5050.2601564029384794No Hit
CAGGAAANAGCTATGANNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT5010.2580957581627291No Hit
NAGGAAANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC4860.2503683402536654No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA4560.23491350443553788No Hit
CAGGAAANNGCTATGANNCCGTAGCCACGCCCCTATTGTCTGACCACGCT4550.2343983432416003No Hit
CAGGANANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC4540.23388318204766273No Hit
CAGGAAANAGCTATGANNTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC4450.22924673130222448No Hit
CAGGAAANAGCTATGANNAGATCCTGTGAAGTGGGAGGGTTTTCAAAGTC4270.21997382981134797No Hit
NAGGAAANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC4260.21945866861741037No Hit
NAGGANANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC4180.21533737906590972No Hit
CAGGAAANNGCTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA4110.21173125070834664No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA4100.21121608951440904No Hit
CAGGAAANNGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT4080.2101857671265339No Hit
CAGGAAANAGCTATGANNGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG4000.20606447757503324No Hit
CAGGAAANAGCTATGANNGGGTCCCACCCATGCTGAAAGTCCACCTTCCA3900.20091286563565738No Hit
CAGGANANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC3880.19988254324778224No Hit
CAGGAAANAGCTATGANNCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC3870.19936738205384463No Hit
CAGGAAANNGCTATGANNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT3750.19318544772659366No Hit
CAGGAAANAGCTATGANNTCCAGCTGCTCCATCTTGTCTACCTCCTGGAA3680.18957931936903058No Hit
NAGGANANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC3680.18957931936903058No Hit
CAGGAAANAGCTATGANNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG3670.189064158175093No Hit
CAGGAAANAGCTATGANNAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG3650.1880338357872178No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA3570.18391254623571715No Hit
CAGGAAANAGCTATGANNAGTGGGAGGAGCTCTCGGTCCCCTCTTTTAGG3530.18185190145996682No Hit
CAGGAAANNGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC3340.17206383877515274No Hit
CAGGAAANAGCTATGANNGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC3270.16845771041758967No Hit
CAGGAAANNGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC3220.16588190444790174No Hit
CAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATCCAAACCAG3170.16330609847821384No Hit
CAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3090.15918480892671316No Hit
CAGGAAANNGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA3020.15557868056915009No Hit
CAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2990.15403319698733733No Hit
NAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2920.15042706862977426No Hit
CAGGAAANNGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG2850.14682094027221118No Hit
NAGGANANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2810.14476029549646083No Hit
CAGGAAANAGCTATGANNATCGCACCACTGCACTCCAGCGCTCCATACAC2780.1432148119146481No Hit
NAGGAAANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2750.14166932833283535No Hit
NAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2750.14166932833283535No Hit
CAGGANANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA2710.139608683557085No Hit
CAGGAAANAGCTATGANNGTGCTCCTTGGGGACGTAGTCGCCTGGGGACT2690.13857836116920985No Hit
CAGGAAACNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC2660.1370328775873971No Hit
NAGGAAANNGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT2650.1365177163934595No Hit
CAGGAAANAGCTATGANNGCAAAACACCTTAACACTCGGCTTCTATTTGC2640.13600255519952192No Hit
NAGGANANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA2590.13342674922983402No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT2580.13291158803589645No Hit
CAGGAAANAGCTATGANNCAGAAGGCTGAGCAGGAGGCTGGGAAAAAGGG2560.13188126564802125No Hit
NAGGANANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA2510.12930545967833335No Hit
CAGGAAANNGCTATGANNAGCGTCTCATTCCATAAAGGCCACCATGAAAC2500.12879029848439577No Hit
NAGGANANNGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT2490.1282751372904582No Hit
CAGGAAACAGCTATGANCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC2480.1277599760965206No Hit
CAGGAAANNGCTATGANNGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG2480.1277599760965206No Hit
CAGGAAANNGCTATGANNTGCTGGGAGAGGGGGATGAGCGGCGCCGCCAT2480.1277599760965206No Hit
CAGGAANNNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC2420.1246690089328951No Hit
CAGGAAANAGCTATGANNAGGTCCAGATGGCTGGTGACTGGCCGGGGTGG2420.1246690089328951No Hit
NAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC2380.12260836415714477No Hit
CAGGANANNGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT2370.12209320296320718No Hit
NAGGAAANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA2350.12106288057533203No Hit
NAGGAAANAGCTATGANCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC2350.12106288057533203No Hit
CAGGAAANNGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG2320.11951739699351928No Hit
CAGGAAACNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC2310.11900223579958168No Hit
NAGGANANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA2300.1184870746056441No Hit
CAGGAAANNGCTATGANNGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC2290.11797191341170651No Hit
CAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG2280.11745675221776894No Hit
CAGGAAANNGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA2280.11745675221776894No Hit
CAGGAAANAGCTATGANNTCTGACTGTCCGTGATCCCACACAATCACCCT2240.11539610744201861No Hit
CAGGANANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2230.11488094624808101No Hit
CAGGAANNNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC2190.11282030147233069No Hit
CAGGAAACAGCTATGANCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT2160.11127481789051795No Hit
CAGGAAACAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2150.11075965669658037No Hit
NAGGAAANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA2130.10972933430870518No Hit
NAGGAAANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA2110.10869901192083002No Hit
NAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC2110.10869901192083002No Hit
CAGGAAACNGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT2080.10715352833901728No Hit
NAGGAAANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA2050.10560804475720452No Hit
CAGGAAACAGCTATGANCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC2040.10509288356326695No Hit
CAGGANANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA2030.10457772236932936No Hit
CAGGAAACNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2010.1035473999814542No Hit
CAGGAAANNGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT2010.1035473999814542No Hit
CAGGAAANNGCTATGANNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT1990.10251707759357905No Hit
CAGGANANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA1990.10251707759357905No Hit
CAGGAAANNGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1990.10251707759357905No Hit
CAGGAAANNGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG1980.10200191639964146No Hit
CAGGAAANAGCTATGANNTGCTGCCGAGAGAGGACAGTGATCCCCCAAAA1980.10200191639964146No Hit
NAGGANANNGCTATGANNGTCCTAGCACCGCAGCGAATGGGACCCGCAAG1970.10148675520570385No Hit
CAGGAAANNGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG1960.10097159401176628No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)