FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003246258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23837
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGAGGGGTATAT960.40273524352896756No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC910.38175944959516717No Hit
NNGGANNNNGNTATGNNNACAGTGTCCCCATCAGGCTCCGGGACAGCATC910.38175944959516717No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC670.2810756387129253No Hit
NNGGANNNNGNTATGNNNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG660.2768804799261652No Hit
NAGGAANNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC590.24751436841884467No Hit
NNGGANNNNGNTATGNNNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA570.2391240508453245No Hit
NNGGANNNNGNTATGNNNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA570.2391240508453245No Hit
NNGGANNNNGNTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT560.23492889205856443No Hit
NNGGANNNNGNTATGNNNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG550.23073373327180433No Hit
NNGGANNNNGNTATGNNNTGGAGTTTGAAACAGCACCTTGGCACTCCTTC550.23073373327180433No Hit
NAGGAAANAGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT510.213953098124764No Hit
NNGGANNNNGNTATGNNNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT500.20975793933800396No Hit
NNGGANNNNGNTATGNNNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA490.20556278055124388No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC430.18039182783068342No Hit
NNGGANNNNGNTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA430.18039182783068342No Hit
NAGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGAGGGGTATAT420.1761966690439233No Hit
NAGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC410.17200151025716323No Hit
NNGGANNNNGNTATGNNNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT400.16780635147040315No Hit
NNGGANNNNGNTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC400.16780635147040315No Hit
NNGGAAANNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC390.16361119268364308No Hit
CAGGAAANAGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT390.16361119268364308No Hit
NAGGANNNNGNTATGNNNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA390.16361119268364308No Hit
NAGGAANNNGNTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT380.159416033896883No Hit
NNGGANANAGNTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT370.15522087511012292No Hit
NNGGANNNNGNTATGNNNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC360.15102571632336284No Hit
NNGGANNNNGNTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA360.15102571632336284No Hit
NNGGANANAGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC340.14263539874984268No Hit
NAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC340.14263539874984268No Hit
NAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC330.1384402399630826No Hit
NAGGANNNNGNTATGNNNACAGTGTCCCCATCAGGCTCCGGGACAGCATC330.1384402399630826No Hit
NAGGAANNNGNTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA330.1384402399630826No Hit
CAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC330.1384402399630826No Hit
NNGGANNNNGNTATGNNNAGGCTTTGTGTGTGGGGACTGCAGTGAACTGA320.13424508117632253No Hit
NNGGANANAGNTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC310.13004992238956242No Hit
NNGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG300.12585476360280237No Hit
CAGGAAANAGCTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA300.12585476360280237No Hit
NNGGAAANNGNTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT300.12585476360280237No Hit
NAGGAANNNGNTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG290.12165960481604228No Hit
NAGGAANNNGNTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC290.12165960481604228No Hit
NNGGANNNNGNTATGNNNTGCTGCCGAGAGAGGACAGTCCCCAACCATAG290.12165960481604228No Hit
NNGGANNNNGNTATGNNNTGGAGTTTGAAACAGCACCTTGGGTCCTCCTC290.12165960481604228No Hit
NAGGAAANAGCTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA290.12165960481604228No Hit
NNGGANANAGNTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG280.11746444602928222No Hit
NAGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC280.11746444602928222No Hit
NNGGANANAGNTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC280.11746444602928222No Hit
NAGGAANNNGNTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA280.11746444602928222No Hit
NNGGANNNNGNTATGNNNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA270.11326928724252212No Hit
CAGGAAANAGCTATGANNTGCTGCCGAGAGAGGACAGTCCCCAACCATAG270.11326928724252212No Hit
NNGGANANAGNTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA270.11326928724252212No Hit
NNGGANNNNGNTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG270.11326928724252212No Hit
NNGGANNNNGNTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG270.11326928724252212No Hit
NNGGAAANNGNTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA260.10907412845576205No Hit
NNGGANNNNGNTATGNNNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC260.10907412845576205No Hit
NNGGANNNNGNTATGANNTGGAGTTTGAAACAGCACCTTGGCACTCCTTC260.10907412845576205No Hit
NNGGANANAGNTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT260.10907412845576205No Hit
NNGGANNNNGNTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC250.10487896966900198No Hit
NAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA250.10487896966900198No Hit
NAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG250.10487896966900198No Hit
CAGGAAANAGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG250.10487896966900198No Hit
NNGGANNNNGNTATGNNNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA240.10068381088224189No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)