FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003246262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179019
Sequences flagged as poor quality0
Sequence length300
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCAGGCAGTAACTTGGGCATTTCTTCTGATTCTC38782.1662505097224316No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA38182.1327345142135754No Hit
CAGGAAACAGCTATGACCGGGGAGACTGGGCCATACGATAGGCTTAATCC34951.9523067383908972No Hit
CAGGAAACAGCTATGACCAGCCTCTCCCCTCGTTCCAGCAACTCAGTGAG34511.9277283416844024No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCTGCCCAACACCC32881.8366765538853418No Hit
CAGGAAACAGCTATGACCAGAGGCACAGGTGGAGAGGGACGAGACCCTGA27591.541177193482256No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA27501.5361497941559275No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT26291.4685592032130668No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAACCATGTGCCC22851.2764008289622888No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGCACTCCTTC22641.2646702305341893No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC22191.2395332339025467No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA20801.1618878443070288No Hit
CAGGAAACAGCTATGACCTGGAAACCCATGCCATGCACTCAACACTACAA19781.1049106519419727No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC18851.0529608589032449No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCAGGAAACAGCT18031.0071556650411408No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC17440.9741982694574319No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGACACTGCCCACC17160.9585574715532988No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG16490.921131276568409No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGGACACTGCCCAC16190.9043732788139806No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTCCCCAACCATAG16180.903814678888833No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT15930.8898496807601427No Hit
CAGGAAACAGCTATGACCGGCTGGCACAAAGAACAGCACTGACTGTTTAT15720.878119082332043No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCAGAGCCACC15460.863595484278205No Hit
CAGGAAACAGCTATGACCATCCACTGTTGGCCAACACAGGGGCGATAGTG15220.8501890860746625No Hit
CAGGAAACAGCTATGACCTGGCATCCAGGGCCTATTTGGTTCCTATTGAG14140.7898602941587205No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT13710.7658404973773734No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG13490.7535512990241259No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGGCACGGGAACA13490.7535512990241259No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCCGGAGAGCCTGT13470.7524340991738308No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGCTCCTCCTCGC13100.7317659019433691No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCACCCCACCCTGCT13090.7312073020182215No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAGGCCCCTGTACC12560.7016015059853982No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG12510.6988085063596602No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG12470.6965741066590697No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACCCCGTTAGCTGC12120.6770231092789033No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG11860.6624995112250655No Hit
CAGGAAACAGCTATGACCCCAGGAGAGAGCAGCCTTCCTCACACCCGTGA11340.6334523151173898No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG11000.6144599176623711No Hit
CAGGAAACAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG10900.608873918410895No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA10700.5977019199079427No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA10540.588764321105581No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT10420.5820611220038097No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC10320.5764751227523336No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGCTCAAGG10300.5753579229020384No Hit
CAGGAAACAGCTATGACCCAGGGTGACTCCGATCCTGGAGCCAACGGTGC10220.5708891235008575No Hit
CAGGAAACAGCTATGACCTCCAGCCAGCCAGTCCTTGTGTTGATGGCCTC10010.5591585250727577No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCTCCCCGCCAAGA9850.5502209262703959No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA9740.5440763270937722No Hit
CAGGAAACAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG9700.5418419273931817No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGCAGGGGCATCCGG9480.5295527290399343No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCACAGGAAACAGC9310.5200565303124249No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT9090.5077673319591776No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA9020.5038571324831442No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACGGCCCCTCTGCC8970.5010641328574061No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTCAGGAATCATGC8790.4910093342047492No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGACACCCAAGACA8780.4904507342796016No Hit
CAGGAAACAGCTATGACCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG8360.466989537423402No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA8120.4535831392198594No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTGCAGTGAACTGA7730.4317977421391025No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGTCTGTGGGCGGA7660.42788754266306933No Hit
CAGGAAACAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG7510.4195085437858551No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAACAGCAGACACC7340.4100123450583458No Hit
CAGGAAACAGCTATGACCGATGCTCCTTCCTTGGCCCCAGGCCTTGGCGT7300.4077779453577553No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGGCTGTTT6870.3837581485764081No Hit
CAGGAAACAGCTATGACCTTGGACAGCACCTTGAATGCTTTCAGGGAAGC6680.3731447499986035No Hit
CAGGAAACAGCTATGACCGGCAGACAGCCTCTCCTCCTTTCATCACAAAA6620.3697931504477179No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT6400.35750395209447045No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGGCTGCTT6330.3535937526184372No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCGCCCAACACCC6190.34577335366637063No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGCTGTCCTCCTC6130.3424217541154849No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAGTGCCCCACTG6100.3407459543400421No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCCTGCCCCCAGA6030.33683575486400885No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCAGAGGGAGCTAGG5880.3284567559867947No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC5820.32510515643590904No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGTCAGGTCCAAGT5760.3217535568850234No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCAGATTTTCAAT5690.3178433574089901No Hit
CAGGAAACAGCTATGACCAGAGACCAGATGCATAAGCACAGCAGCATTCC5650.3156089577083997No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCCTCTCCCAACT5550.31002295845692357No Hit
CAGGAAACAGCTATGACCTTGGACAGCACCTTGAATGCCTGGGTTCTAAC5520.3083471586814807No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCTTCCCATCTCA5450.3044369592054475No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGATTCTCAGATTCC5410.302202559504857No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCAGACGCACGGC5360.299409559879119No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG5350.2988509599539714No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACTGGATCCGCTTC5310.2966165602533809No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT5310.2966165602533809No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCAAGCCTGACTG5160.2882375613761668No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTGCCCACACGGGC5100.2848859618252811No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCAGCACAGGGAGT5090.28432736190013347No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCGCAGTGGGGCCA4980.2781827627235098No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAACAGTGAAGACAG4960.27706556287321454No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGCGGAAGAGGAGCC4960.27706556287321454No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT4910.27427256324747656No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCCCCGGGCAGA4840.27036236377144324No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGGCTCTTTT4820.26924516392114806No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACGGGGATGGCAGTG4800.2681279640708528No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCGTGTGCGCTGGA4780.2670107642205576No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCCAGAGAGCACCCG4780.2670107642205576No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTAGGGTCTGGGGC4710.2631005647445243No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGACACACCACT4700.26254196481937675No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGC4550.25416296594216253No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGCTCACTGGTGTC4500.2513699663164245No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACAGAGGAGCCGGT4330.24187376758891513No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGCAAAGGGGAACAAGC4330.24187376758891513No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCTCCGTGTGCGCT4320.24131516766376754No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCCAGCTAAGTGA4270.2385221680380295No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCCCACAGAGAGCAC4240.23684636826258662No Hit
CAGGAAACAGCTATGACCAGAGGCACAGGTGGAGAGGGGGTGATGGCCAT4210.23517056848714382No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGCCTGCCCAGGCC4100.2290259693105201No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTCCACACTGGCCG3850.21506097118182987No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTCCTGTGCCCATC3820.21338517140638705No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTGGGGGAGGGAGA3790.2117093716309442No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGACCTCTCCTCCT3780.21115077170579657No Hit
CAGGAAACAGCTATGACCTCCAGCCAGCCAGTCCTTGTCCTTATATCTTT3690.2061233723794681No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCAGCTCCCCAGG3630.20277177282858244No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGACGCTGCCCTGC3620.20221317290343485No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCAGCTCCGA3550.19830297342740155No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT3500.19550997380166352No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCCAGGAAACAGCA3470.1938341740262207No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTGAGATCACACCA3450.19271697417592545No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCACCCCAGCAAGA3440.19215837425077786No Hit
CAGGAAACAGCTATGACCGATGCTCCTTCCTTGGCCCCGGTACAGTAACT3340.18657237499930174No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAGGGGACATGGCC3300.1843379752987113No Hit
CAGGAAACAGCTATGACCTGCTGCTTTTCCTGCCAGAACAGGAAACAGAA3280.18322077544841608No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACAGGAGGGTCCATC3230.18042777582267805No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA3230.18042777582267805No Hit
CAGGAAACAGCTATGACCTGCTGCTTTTCCTGCCAGAAGACAGAAGCCCA3170.17707617627179237No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCCCGTGTGCGCTG3130.17484177657120192No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCCTGCCCCCTGCA3130.17484177657120192No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGGTACTAATGAGG3080.1720487769454639No Hit
CAGGAAACAGCTATGACCAGAGGCACAGGTGGAGAGGGACGAGACCATGA2970.16590417776884017No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCAGTTCATGCAGT2900.1619939782928069No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTGTGGCTGGTGTA2860.15975957859221646No Hit
CAGGAAACAGCTATGACCTGCTGCTTTTCCTGCCAGAAGCTCGAACTGGG2860.15975957859221646No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTGCGGGGAGCTGA2810.15696657896647842No Hit
CAGGAAACAGCTATGACCACCTGGCAGCCCCAAATCTGCACCTCCCGCAG2780.1552907791910356No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGTCACTGCAGCCTGGG2770.15473217926588798No Hit
CAGGAAACAGCTATGACCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG2750.15361497941559277No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT2720.15193917964014994No Hit
CAGGAAACAGCTATGACCACCTGGCAGCCCCAAATCTGCACCTCCCGCGG2710.1513805797150023No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGTGGTGGGCCACA2670.14914618001441188No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCTGTCACACACCC2630.14691178031382143No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCTTCCTGCAACAC2630.14691178031382143No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGACCTGTGCCC2590.144677380613231No Hit
CAGGAAACAGCTATGACCTCCAGCCAGCCAGTCCTTGTTGATGGCCTCAG2570.14356018076293578No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCCGGGCAGAGG2510.14020858121205013No Hit
CAGGAAACAGCTATGACCGGGCAGGCCGAGGACATTAGAAATTTGGGACG2480.13853278143660727No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTTATAATCCCAGC2390.1335053821102788No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACACAGCTGGGAGGC2350.13127098240968837No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT2230.12456778330791704No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA2190.1223333836073266No Hit
CAGGAAACAGCTATGACCAACACCCTTGGCCTCCCTCACTGGCCGTCGTT2120.11842318413129331No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCTCGGCCGTAAGG2100.1173059842809981No Hit
CAGGAAACAGCTATGACCCCCATCCACTAGTCGGGGAGTAGGAGGCTGTC1930.10780978555348873No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGACAGGAAACAGCT1930.10780978555348873No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTGGTGGTGGACTG1900.10613398577804589No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCTATTTTCCAAAG1860.10389958607745547No Hit
CAGGAAACAGCTATGACCCATGTGCCCCACTGATGCTCTCAATGTGTACT1850.10334098615230784No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACTGGCCGTCGTTT1840.10278238622716024No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGGAAATAACAGGC1830.10222378630201263No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCAGGCAAACTTGG1810.10110658645171743No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCGGGTCTTACA1810.10110658645171743No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)