FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122091
Sequences flagged as poor quality0
Sequence length300
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCTGGAAACCCATGCCATGCACTCAACACTACAA61325.022483229722092No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACCCCGTTAGCTGC43973.601412061495114No Hit
CAGGAAACAGCTATGACCGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT34912.8593426214872513No Hit
CAGGAAACAGCTATGACCAGCGTCTCATTCCATAAAGGCCACCATGAAAC31762.6013383459878288No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA30892.5300800222784643No Hit
CAGGAAACAGCTATGACCTCCCTTAAACTTGGGATTGCTCCATTGCACTC28802.3588962331375773No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCAGCACAGGGAGT24822.0329098786970374No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC22731.8617260895561507No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT21951.7978393165753412No Hit
CAGGAAACAGCTATGACCACCTTTACCCAAATAGCTACCACCAGTGCCTT20891.7110188302168057No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT19861.6266555274344545No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTCCCCAACCATAG18481.5136250829299458No Hit
CAGGAAACAGCTATGACCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC18281.4972438590887125No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG17731.4521954935253214No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCAGAGCCACC17591.440728636836458No Hit
CAGGAAACAGCTATGACCTGACAGCACAAGGAACACTGACCCGAGGCAGG17221.4104233727301767No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGCTCCTCCTCGC16541.3547272116699838No Hit
CAGGAAACAGCTATGACCCCCATCCACTAGTCGGGGAGTTGCTTGTCATT15171.2425158283575366No Hit
CAGGAAACAGCTATGACCTCTACGCCCCTCAGCTGTGTCCTGTCTGCATA14651.1999246463703304No Hit
CAGGAAACAGCTATGACCTTGCCAGTGCTGCCTTCAGGGACTGGCCGTCG13901.138495056965706No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC13761.1270282002768428No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC13421.0991801197467463No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCCATGCTCACCT13051.068874855640465No Hit
CAGGAAACAGCTATGACCGATGGCAGAATGGAAACATGGCAGCTGCCTTG12871.054131754183355No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG12170.9967974707390389No Hit
CAGGAAACAGCTATGACCATCGCACCACTGCACTCCAGCGCTCCATACAC11320.9271772694137979No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAACCATGTGCCC10810.8854051486186534No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCAGCTCCGA10260.8403567830552621No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCTGCCAGGAGCAGG9670.7920321727236241No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGCCCCGGGAGCTG8750.7166785430539515No Hit
CAGGAAACAGCTATGACCAGGACTTGCCCACTCTCCCAGGAAGCAGAAGT8500.6962020132524102No Hit
CAGGAAACAGCTATGACCTCCCTCATTGTTGAGAGGCAGCAGTTGCAGAA8300.6798207894111769No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA8050.6593442596096354No Hit
CAGGAAACAGCTATGACCCGTAAAGGGCAAGGTGGGGTAATCTTGTCCAT7960.6519727088810805No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGCCCGGACGAGGA7940.6503345864969572No Hit
CAGGAAACAGCTATGACCGATGCTCCTTCCTTGGCCCCAGGCCCTGGCGT7920.6486964641128339No Hit
CAGGAAACAGCTATGACCGGCGGGCCCTGATATAGAGCCACTGGCCGTCG7710.631496179079539No Hit
CAGGAAACAGCTATGACCAGGACTTGCCCACTCTCCCACAGGAAGCAGAA7540.6175721388144908No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACCAGGAAACAGCT7520.6159340164303675No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGAGCTGCGAGCAT7450.6102005880859359No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTGGCAGCTGCAC7270.595457486628826No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCGTGTTCTCACTC7030.5758000180193462No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCTTCCCATCTCA6850.5610569165622364No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACCCTTTGTCCA6710.5495900598733732No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCACAGGAAACAGC6220.5094560614623519No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCAGGCAC6090.4988082659655503No Hit
CAGGAAACAGCTATGACCGATGCTCCTTCCTTGGCCCCGGTACTGGCAGT5860.4799698585481321No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCCTCTCCCAACT5840.4783317361640088No Hit
CAGGAAACAGCTATGACCGATGGCAGAATGGAAACATGGCACCTTGCTTC5810.47587455258782385No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGGCACGGGAACA5370.4398358601371108No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCAGCCAGGAGCAGG5200.4259118198720626No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGGGAGGGAGCTGC5040.41280684079907615No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGCCTGCCCAGGCC4980.4078924736467061No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCATGCTGGGTGGGA4830.39560655576578124No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGTCTGCAGCCTGG4720.38659688265310305No Hit
CAGGAAACAGCTATGACCCCCATCCACTAGTCGGGGAGTAGGAGGCTGTC4690.38413969907691803No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG4660.38168251550073307No Hit
CAGGAAACAGCTATGACCACCCCACCTGCTCAAAAGACACACTCCTAAAA4240.34728194543414337No Hit
CAGGAAACAGCTATGACCATCGCACCACTGCACTCCAGTGGCGCTCCATA4130.3382722723214651No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGCATCCTGG4120.33745321112940346No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTGCCATGCTCACC3800.3112432529834304No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGGGCTGAAGAAGA3770.3087860694072454No Hit
CAGGAAACAGCTATGACCATCGCACCACTGCACTCCAGCCACTGCGCTCC3340.2735664381485941No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGTCTGTGGGCGGA3280.2686520709962241No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT3250.26619488742003916No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT3130.25636615311529926No Hit
CAGGAAACAGCTATGACCCCCATCCACTAGTCGGGGAGTGTCATTGGTGC3010.24653741881055935No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT2930.23998492927406606No Hit
CAGGAAACAGCTATGACCAGCGTCTCATTCCATAAAGGCCACCATTCACC2900.2375277456978811No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCGTGCTGGGCCA2810.23015619496932616No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGGGGGCCTGGCT2790.22851807258520285No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGGAGCTGCGAGCA2790.22851807258520285No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTACCATGCTCACC2770.2268799502010795No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGCTGTCCTCCTC2690.22032746066458622No Hit
CAGGAAACAGCTATGACCACCTTTACCCAAATAGCTACCACCACACAGGA2620.21459403232015464No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGCCTGGCTTGTC2560.2096796651677847No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG2480.2031271756312914No Hit
CAGGAAACAGCTATGACCGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG2390.19575562490273649No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCACAGGTCCCACCC2370.19411750251861318No Hit
CAGGAAACAGCTATGACCACCTTTACCCAAATAGCTACCACCACTGGCAA2350.19247938013448984No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTGACCCTGGCAG2300.18838407417418154No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCAGCTCTGA2280.18674595179005823No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCACCCAGCACAGG2220.18183158463768828No Hit
CAGGAAACAGCTATGACCACTCCCTTGAGCCTTTGGGGAAAGGGAGACAG2200.18019346225356497No Hit
CAGGAAACAGCTATGACCGCCCCTACTCCAAAAGGCGGCAGAGAGTGGAG2150.17609815629325667No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCCGTCCTCCCAGC2090.17118378914088672No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTAGGGTCTGGGGC2060.16872660556470173No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC2050.16790754437264008No Hit
CAGGAAACAGCTATGACCCTGGGTGCCGGGTAGAGTGTCAGGAAACAGTC2010.16463129960439343No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCAGGCCAGGAGCAG2000.16381223841233178No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGACAAGTGCATGG1970.16135505483614682No Hit
CAGGAAACAGCTATGACCGAAAGGCTTCCCCAGGAGCCTCCCTTAGGAGT1940.15889787125996185No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCAGTTCTCACTCT1920.15725974887583852No Hit
CAGGAAACAGCTATGACCGATGCTCCTTCCTTGGCCCCAGGCCCTAGCGT1870.15316444291553022No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT1800.14743101457109858No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGATGCCACG1740.14251664741872866No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCGTGCTAGGCCA1710.14005946384254367No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCACCCCCCCATC1710.14005946384254367No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCCTGGCTTGTCC1600.13104979072986542No Hit
CAGGAAACAGCTATGACCGCCCCTACTCCAAAAGGCGGTAGAGAGTGGAG1510.1236782400013105No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCCAGGACGTGTCA1500.12285917880924885No Hit
CAGGAAACAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC1440.1179448116568789No Hit
CAGGAAACAGCTATGACCATCGCACCACTGCACTCCAGCCTGCGCTCCAT1390.11384950569657058No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTCAGGAAACA1390.11384950569657058No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCGTCCATGCTCA1340.10975419973626228No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGGTGTGGTCGGCA1330.10893513854420063No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTTGGAGTCCCAC1330.10893513854420063No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA1330.10893513854420063No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCCCACAGAGAGCAC1320.10811607735213898No Hit
CAGGAAACAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC1300.10647795496801565No Hit
CAGGAAACAGCTATGACCATCGCACCACTGCACTCCAGCCTGGGTGACAG1290.105658893775954No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCAGACACACGGC1280.10483983258389235No Hit
CAGGAAACAGCTATGACCATCGCACCACTGCACTCCAGCCTAGGCATTGT1270.10402077139183069No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1270.10402077139183069No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCCCTGTCATCCCAC1250.10238264900770735No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCCACAGAGCCAGGC1240.1015635878156457No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGATTCCCCTCGCGG1240.1015635878156457No Hit
CAGGAAACAGCTATGACCATCGCACCACTGCACTCCAGCCTGGGCAACAG1230.10074452662358405No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)