FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101589
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT4740.4665859492661607No Hit
NNGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATTCATCGTCT4380.4311490417269586No Hit
NNGGANNNNGNTATGANNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT3970.3907903414739785No Hit
NNGGANNNNGNTATGNNNCGACATATCCGAGCCTCACGTTCTAGTTTCTG3890.3829154731319336No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT3880.38193111458917794No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAATT3630.35732215102028764No Hit
NNGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3600.3543690753920208No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC3210.3159790922245519No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC3100.3051511482542401No Hit
NNGGANNNNGNTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2910.28644833594188346No Hit
NNGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2860.28152654322810544No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT2800.27562039197157173No Hit
NNGGANNNNGNTATGNNNTGGGGATGAGGACAGCAGGGCAGTCACCATCT2760.2716829578005493No Hit
NNGGANNNNGNTATGNNNAGAGGCACAGGTGGAGAGGGACGAGACCCTGA2670.2628237309157488No Hit
NNGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGACACTGCCCACC2650.26085501383023757No Hit
NNGGANNNNGNTATGNNNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA2650.26085501383023757No Hit
NNGGANNNNGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG2600.25593322111645944No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT2590.25494886257370386No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG2440.24018348443236964No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC2420.23821476734685842No Hit
NNGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGGACACTGCCCAC2320.22837118191930228No Hit
NAGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT2260.22246503066276863No Hit
NNGGANNNNGNTATGNNNAGACAGGGTCTTGCTATGTTGCCCAGGTTGAT2250.22148067212001302No Hit
NNGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2170.2136058037779681No Hit
NNGGANNNNGNTATGNNNGGGGAGACTGGGCCATACGATAGGCTTAATCC2150.21163708669245687No Hit
NNGGANNNNGNTATGNNNCCACCCTCATGCCTAACCCCAGCACAGGGAGT2110.2076996525214344No Hit
NAGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2100.2067152939786788No Hit
NNGGANNNNGNTATGNNNGCTGCTGACCCAACCCTACCTCTGCAGCTAGC2070.20376221835041197No Hit
NNGGANNNNGNTATGNNNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC2060.20277785980765636No Hit
NNGGANNNNGNTATGNNNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT2060.20277785980765636No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2030.19982478417938948No Hit
NNGGANNNNGNTATGNNNTGACTGAAAGGCTTTTCCACGTGCACTGCTGG1930.18998119875183336No Hit
NNGGANNNNGNTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC1930.18998119875183336No Hit
NNGGANNNNGNTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1920.18899684020907775No Hit
NNGGANNNNGNTATGNNNTGACAGCACAAGGAACACTGACCCGAGGCAGG1880.18505940603805532No Hit
NAGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1860.18309068895254407No Hit
NNGGANNNNGNTATGNNNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT1840.18112197186703285No Hit
NNGGANNNNGNTATGANNAGAGGCACAGGTGGAGAGGGACGAGACCCTGA1820.1791532547815216No Hit
NNGGANNNNGNTATGNNNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG1810.178168896238766No Hit
NAGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAATT1790.17620017915325478No Hit
NNGGANNNNGNTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1780.17521582061049917No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG1730.1702940278967211No Hit
NAGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT1720.1693096693539655No Hit
NNGGANNNNGNTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT1710.16832531081120988No Hit
NNGGANNNNGNTATGNNNCATGGTTGGGGAAAAACTCCTCATCTTCATGT1690.16635659372569866No Hit
NNGGANNNNGNTATGANNCCACCCTCATGCCTAACCCCAGCACAGGGAGT1680.16537223518294306No Hit
NNGGANNNNGNTATGANNCACGACACCCCACCTCTTGGACACTGCCCACC1670.16438787664018742No Hit
NNGGANNNNGNTATGANNGCTGCTGACCCAACCCTACCTCTGCAGCTAGC1650.1624191595546762No Hit
NAGGANNNNGNTATGNNNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1640.1614348010119206No Hit
NAGGANNNNGNTATGANNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT1580.1555286497553869No Hit
NNGGANNNNGNTATGNNNATCCACTGTTGGCCAACACACCTGGCCTTCAA1560.15355993266987567No Hit
NNGGANNNNGNTATGANNAGACAGGGTCTTGCTATGTTGCCCAGGTTGAT1530.15060685704160884No Hit
NNGGANNNNGNTATGANNGGGGAGACTGGGCCATACGATAGGCTTAATCC1520.1496224984988532No Hit
NNGGANNNNGNTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT1520.1496224984988532No Hit
NAGGANNNNGNTATGNNNGCTGCTGACCCAACCCTACCTCTGCAGCTAGC1510.1486381399560976No Hit
NNGGANNNNGNTATGNNNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1480.14568506432783077No Hit
NNGGANNNNGNTATGNNNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC1470.14470070578507516No Hit
NAGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1450.14273198869956394No Hit
NNGGANNNNGNTATGANNTGACAGCACAAGGAACACTGACCCGAGGCAGG1440.14174763015680833No Hit
NNGGANNNNGNTATGANNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG1440.14174763015680833No Hit
NAGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1410.13879455452854148No Hit
NAGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC1390.13682583744303026No Hit
NNGGANNNNGNTATGNNNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA1380.13584147890027465No Hit
NNGGANNNNGNTATGANNTGACTGAAAGGCTTTTCCACGTGCACTGCTGG1300.12796661055822972No Hit
NNGGANNNNGNTATGNNNACCTCTCTGGGCCTGTCTCCTCCCCGCCAAGA1300.12796661055822972No Hit
NNGGANNNNGNTATGNNNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG1300.12796661055822972No Hit
NAGGANNNNGNTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1290.1269822520154741No Hit
NNGGANNNNGNTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC1280.1259978934727185No Hit
NNGGANNNNGNTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT1270.1250135349299629No Hit
NAGGANNNNGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1260.12402917638720727No Hit
NNGGANNNNGNTATGNNNCCCTGCGGCTCCTTTCAAGTGCAGAGGAAGCC1250.12304481784445166No Hit
NNGGANNNNGNTATGNNNCTCACCAAGAGTGCCCCACGCCCAAGACACTG1250.12304481784445166No Hit
NNGGANNNNGNTATGNNNGATGCTCCTTCCTTGGCCCCGGTACTGGCAGT1240.12206045930169605No Hit
NNGGANNNNGNTATGNNNAGACAGGGTCTTGCTATGTTGCCAAAGGAGAT1210.11910738367342921No Hit
NNGGANNNNGNTATGNNNAAGGACAAGGTGAGGCAGCACCCCACCCTGCT1200.1181230251306736No Hit
NAGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT1200.1181230251306736No Hit
NNGGANNNNGNTATGNNNCAGGGTGACTCCGATCCTGGAGCCAACGGTGC1190.117138666587918No Hit
NNGGANNNNGNTATGANNAGGGCAAGTGTTCGCGTTGTGACAGTTACGGT1180.11615430804516239No Hit
NNGGANNNNGNTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1180.11615430804516239No Hit
NAGGANNNNGNTATGNNNAGAGGCACAGGTGGAGAGGGACGAGACCCTGA1180.11615430804516239No Hit
NNGGANNNNGNTATGNNNCCCAAACCTGCGGAGGCACACAGCTGGGAGGC1180.11615430804516239No Hit
NAGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGGACACTGCCCAC1170.11516994950240675No Hit
NAGGANNNNGNTATGNNNCCACCCTCATGCCTAACCCCAGCACAGGGAGT1170.11516994950240675No Hit
NNGGANNNNGNTATGNNNCATGGTTGGGGAAAAACTCCTCATTTTCATGT1150.11320123241689553No Hit
NNGGANNNNGNTATGNNNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC1140.11221687387413992No Hit
NNGGANNNNGNTATGNNNTCCTGCCCCACCCTGATACAGGTCACTGTGGA1130.11123251533138431No Hit
NAGGANNNNGNTATGNNNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG1120.11024815678862868No Hit
NNGGANNNNGNTATGNNNGGGCCTTCTCGAAGCTCTCCCAGGAAACAGCT1120.11024815678862868No Hit
NNGGANNNNGNTATGNNNTCCCACCACTGACTCCAGGGGAAGGAGGAGGA1120.11024815678862868No Hit
NAGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT1120.11024815678862868No Hit
NNGGANNNNGNTATGNNNAGCAAAGGTCTTGGCAGGGGTGGGGACTGGAA1110.10926379824587307No Hit
NAGGANNNNGNTATGNNNTGGGGATGAGGACAGCAGGGCAGTCACCATCT1110.10926379824587307No Hit
NNGGANNNNGNTATGNNNAGGGCAAGTGTTCGCGTTGTGACAGTTACGGT1110.10926379824587307No Hit
NNGGANNNNGNTATGANNTCCTGCCCCACCCTGATACAGGTCACTGTGGA1110.10926379824587307No Hit
NNGGANNNNGNTATGNNNCGCTTCTGGGGACTTTGCTCTCCCTTTGGGCT1100.10827943970311746No Hit
NNGGANNNNGNTATGANNCATGGTTGGGGAAAAACTCCTCATTTTCATGT1090.10729508116036185No Hit
NNGGANNNNGNTATGNNNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC1080.10631072261760624No Hit
NNGGANNNNGNTATGNNNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG1080.10631072261760624No Hit
NNGGANNNNGNTATGNNNCTGGTCCTCATCCTTGGGGCCGGAGAGCCTGT1070.10532636407485062No Hit
NAGGANNNNGNTATGNNNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1070.10532636407485062No Hit
NAGGANNNNGNTATGNNNAGACAGGGTCTTGCTATGTTGCCCAGGTTGAT1070.10532636407485062No Hit
NNGGANNNNGNTATGNNNCTCACCAAGAGTGCCCCACGACACCCAAGACA1060.10434200553209501No Hit
NNGGANNNNGNTATGNNNCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT1040.10237328844658379No Hit
NNGGANNNNGNTATGNNNGCCCAGCAATGGAGAGGCTAGCCCACCGCAAA1040.10237328844658379No Hit
NNGGANNNNGNTATGNNNCAGGGTGACTCCGATCCTGGAGGACAAGGCGG1030.10138892990382818No Hit
NNGGANNNNGNTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA1030.10138892990382818No Hit
NNGGANNNNGNTATGNNNCTTGGTGGAGCCATAGGCCGCATGCACGCGCA1030.10138892990382818No Hit
NNGGANNNNGNTATGANNATCCACTGTTGGCCAACACACCTGGCCTTCAA1020.10040457136107254No Hit
NAGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGACACTGCCCACC1020.10040457136107254No Hit
NNGGANNNNGNTATGANNAAGGACAAGGTGAGGCAGCACCCCACCCTGCT1020.10040457136107254No Hit
NNGGANNNNGNTATGANNCATGGTTGGGGAAAAACTCCTCATCTTCATGT1020.10040457136107254No Hit
NNGGANNNNGNTATGANNACCTCTCTGGGCCTGTCTCCTCCCCGCCAAGA1020.10040457136107254No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)