FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003246271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51491
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC9331.8119671398885242No Hit
TGTAAAANGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC9321.8100250529218698No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT4700.9127808743275524No Hit
TGTAAAANGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC4670.9069546134275894No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC4590.8914179176943544No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA4580.8894758307277No Hit
TGTAAAANGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA4510.8758812219611195No Hit
TGTAAAANGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT4400.8545182653279214No Hit
TGTAAAANGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC4200.8156765259948341No Hit
TGTAAAANGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC4160.8079081781282166No Hit
TGTAAAACGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT4110.7981977432949449No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC4000.7768347866617467No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC3990.7748926996950923No Hit
TGTAAAANGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT3980.772950612728438No Hit
TGTAAAACGACGGCCAGTTGAGAGGGACTGGAGAAAGGGAACTTACAGAG3980.772950612728438No Hit
TGTAAAANGACGGCCAGTTGAGAGGGACTGGAGAAAGGGAACTTACAGAG3840.7457613951952768No Hit
TGTAAAANGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC3550.6894408731623002No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC3380.6564253947291759No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG3300.640888698995941No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC3220.6253520032627061No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGC3200.6214678293293974No Hit
TGTAAAANGACGGCCAGTGGGGTGGGGAGTGAGATGGTCCTAGGAAGTAC3110.603989046629508No Hit
TGTAAAACGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT3070.5962206987628906No Hit
TGTAAAANGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGC3060.5942786117962362No Hit
TGTAAAANGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT3060.5942786117962362No Hit
TGTAAAACGACGGCCAGTGGGGTGGGGAGTGAGATGGTCCTAGGAAGTAC3030.5884523508962731No Hit
TGTAAAANGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG3030.5884523508962731No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC3000.58262608999631No Hit
TGTAAAANGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA2970.576799829096347No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC2950.5729156551630382No Hit
TGTAAAANGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC2930.5690314812297295No Hit
TGTAAAANGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC2860.5554368724631489No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC2860.5554368724631489No Hit
TGTAAAANGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC2780.539900176729914No Hit
TGTAAAANGACGGCCAGTGGTGTTACCGTGGTCACCCGCAGGGACACGGA2770.5379580897632597No Hit
TGTAAAANGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC2740.5321318288632965No Hit
TGTAAAANGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC2690.5224213940300246No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA2630.5107688722300985No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC2510.48746382863024607No Hit
TGTAAAANGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA2500.4855217416635917No Hit
TGTAAAANGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC2500.4855217416635917No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA2470.4796954807636286No Hit
TGTAAAANGACGGCCAGTGAGACAGGCTTCTGGGCTGTCCACAACTCTCT2430.4719271328970111No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC2420.4699850459303568No Hit
TGTAAAANGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC2410.4680429589637024No Hit
TGTAAAANGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC2410.4680429589637024No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC2390.46415878503039365No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA2380.4622166980637393No Hit
TGTAAAACGACGGCCAGTGAGACAGGCTTCTGGGCTGTCCACAACTCTCT2370.46027461109708495No Hit
TGTAAAANGACGGCCAGTTCTGGTGTCGCCCATCACTGGCTTCTGGGCTT2360.4583325241304306No Hit
TGTAAAACGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT2330.45250626323046744No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG2320.45056417626381307No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGGCTTCTGGGCTT2310.44862208929715874No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC2310.44862208929715874No Hit
TGTAAAANGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT2300.44668000233050437No Hit
TGTAAAANGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA2220.4311433065972694No Hit
TGTAAAANGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG2210.4292012196306151No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT2210.4292012196306151No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC2210.4292012196306151No Hit
TGTAAAANGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC2190.42531704569730633No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG2160.41949078479734325No Hit
TGTAAAANGACGGCCAGTAGGTCATCCTCCTGCCTCGGCCTCCCAAAGTG2150.4175486978306889No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG2130.4136645238973801No Hit
TGTAAAACGACGGCCAGTGGTGTTACCGTGGTCACCCGCAGGGACACGGA2120.41172243693072574No Hit
TGTAAAANGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG2060.40006991513079954No Hit
TGTAAAACGACGGCCAGTACACATTCTTGTAAAGCATGGGATTGCTGAAT2000.38841739333087333No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG1990.386475306364219No Hit
TGTAAAANGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG1960.3806490454642559No Hit
TGTAAAANGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT1960.3806490454642559No Hit
TGTAAAANGACGGCCAGTACACATTCTTGTAAAGCATGGGATTGCTGAAT1930.3748227845642928No Hit
TGTAAAANGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCAGTGGG1880.36511234973102097No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG1870.3631702627643666No Hit
TGTAAAANGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG1850.35928608883105784No Hit
TGTAAAANGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA1810.3515177409644404No Hit
TGTAAAACGACGGCCAGTAGGTCATCCTCCTGCCTCGGCCTCCCAAAGTG1790.34763356703113163No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA1760.34180730613116855No Hit
TGTAAAANGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG1660.3223864364646249No Hit
TGTAAAANGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG1660.3223864364646249No Hit
TGTAAAANGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG1610.31267600163135306No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCAGTGGG1560.30296556679808123No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG1510.2932551319648094No Hit
TGTAAAANGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCCGTGGG1510.2932551319648094No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCCGTGGG1490.28937095803150065No Hit
TGTAAAANGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG1490.28937095803150065No Hit
TGTAAAANGACGGCCAGTAAACCTGGCCCCACTGCCCTGCCTCTCCCAGG1480.28742887106484627No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG1450.2816026101648832No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG1440.2796605231982288No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC1420.27577634926492006No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGTCATAGCTGTT1390.269950088364957No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG1380.2680080013983026No Hit
TGTAAAANGACGGCCAGTCCCTGTCCTGAGCACCGTTGCTAACCCCCAGT1380.2680080013983026No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT1380.2680080013983026No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCGTGGGTC1370.26606591443164823No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA1370.26606591443164823No Hit
TGTAAAANGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG1350.26218174049833953No Hit
TGTAAAANGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA1350.26218174049833953No Hit
TGTAAAANGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCGTGGGTC1350.26218174049833953No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTATTTCCCTACTT1350.26218174049833953No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCCAAGAGGTGGGG1340.26023965353168516No Hit
TGTAAAANGACGGCCAGTCCGCCCTTTCCAAGCTCACTATTTCCCTACTT1340.26023965353168516No Hit
TGTAAAANGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA1260.24470295779845022No Hit
TGTAAAANGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGTCATAGCTGTT1250.24276087083179584No Hit
TGTAAAANGACGGCCAGTGGAACTGGTGGGCAGTGTCCCAAGAGGTGGGG1250.24276087083179584No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA1210.2349925229651784No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC1200.233050435998524No Hit
TGTAAAACGACGGCCAGTGGATCCAGGTAGGTGTCCAGGTCACTGCCAAT1180.2291662620652153No Hit
TGTAAAACGACGGCCAGTCCCTGTCCTGAGCACCGTTGCTAACCCCCAGT1160.22528208813190653No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCATCAGGTTCCC1140.2213979141985978No Hit
TGTAAAANGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC1140.2213979141985978No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC1140.2213979141985978No Hit
TGTAAAANGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC1140.2213979141985978No Hit
TGTAAAANGACGGCCAGTGGATCCAGGTAGGTGTCCAGGTCACTGCCAAT1120.21751374026528908No Hit
TGTAAAANGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT1120.21751374026528908No Hit
TGTAAAANGACGGCCAGTCATAGCCCAGGTCCAGCAGCCATCAGGTTCCC1090.21168747936532598No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT1080.20974539239867163No Hit
TGTAAAANGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC1080.20974539239867163No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCCTGCCTCTCCCAGG1070.20780330543201725No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGCATTTCCTTGGC990.19226660969878231No Hit
TGTAAAANGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT970.1883824357654736No Hit
TGTAAAANGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC950.18449826183216483No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCAAGAGGTGGGGT940.18255617486551048No Hit
TGTAAAANGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG910.17672991396554738No Hit
TGTAAAANGACGGCCAGTCCACCTGCCAGTGTCTTGGGCATTTCCTTGGC910.17672991396554738No Hit
TGTAAAANGACGGCCAGTTGTGGAAGTCGGGGACATAAGATGATTTTCAC900.174787826998893No Hit
TGTAAAACGACGGCCAGTACCTCATGTCACGTGCGTCCATGTGAAGAGAG890.17284574003223865No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTTGGACCCAGAG880.17090365306558428No Hit
TGTAAAACGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC870.1689615660989299No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT860.16701947913227555No Hit
TGTAAAACGACGGCCAGTTGTGGAAGTCGGGGACATAAGATGATTTTCAC830.16119321823231245No Hit
TGTAAAANGACGGCCAGTGGCTGACGCAGGAGAATTGCTTGGACCCAGAG820.15925113126565807No Hit
TGTAAAANGACGGCCAGTGACCTCATGGGGCGCTTCCGCGTGCTGGGCTG800.15536695733234934No Hit
TGTAAAACGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG800.15536695733234934No Hit
TGTAAAANGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT800.15536695733234934No Hit
TGTAAAACGACGGCCAGTTGATGACCTCTTGGCTAACATACACAACATGA750.1456565224990775No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCACTGCCTAGCAGG750.1456565224990775No Hit
TGTAAAANGACGGCCAGTGGTGGAGGCCCATTGAGGATGGCCGAGCAGGG750.1456565224990775No Hit
TGTAAAANGACGGCCAGTGGAACTGGTGGGCAGTGTCCAAGAGGTGGGGT740.14371443553242313No Hit
TGTAAAANGACGGCCAGTACCTCATGTCACGTGCGTCCATGTGAAGAGAG740.14371443553242313No Hit
TGTAAAANGACGGCCAGTCGGTGCTCTGGTGAGATGGGGGGCCCGGATGT720.1398302615991144No Hit
TGTAAAANGACGGCCAGTGGGTAGGAAGTCGGGAGGCACTGCCTAGCAGG710.13788817463246003No Hit
TGTAAAANGACGGCCAGTTGGCCCACTGTGCTAGCCTAGGATTTTTTTTT700.13594608766580568No Hit
TGTAAAANGACGGCCAGTCTGGGCCTTGGCTTCTTCCTCAATCATCTAGG640.12429356586587947No Hit
TGTAAAANGACGGCCAGTTGATGACCTCTTGGCTAACATACACAACATGA640.12429356586587947No Hit
TGTAAAACGACGGCCAGTTGGCCCACTGTGCTAGCCTAGGATTTTTTTTT630.12235147889922511No Hit
TGTAAAACGACGGCCAGTCTGGGCCTTGGCTTCTTCCTCAATCATCTAGG610.11846730496591637No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGGGGCCCGGATGT610.11846730496591637No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATGGCCGAGCAGAG600.116525217999262No Hit
TGTAAAACGACGGCCAGTCTTGCACTACATGGCCATCAGGGTCTCGTCCC600.116525217999262No Hit
TGTAAAANGACGGCCAGTGGTGGAGGCCCATTGAGGATGGCCGAGCAGAG600.116525217999262No Hit
TGTAAAACGACGGCCAGTAGATCATCCAGCGCACACGGCTGCTGGACAGT570.1106989570992989No Hit
TGTAAAANGACGGCCAGTAGATCATCCAGCGCACACGGCTGCTGGACAGT550.10681478316599018No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATGGCCGAGCAGGG550.10681478316599018No Hit
TGTAAAANGACGGCCAGTCTTGCACTACATGGCCATCAGGGTCTCGTCCC520.10098852226602707No Hit
TGTAAAACGACGGCCAGTGACCTCATGGGGCGCTTCCGCGTGCTGGGCTG520.10098852226602707No Hit
TGTAAAANGACGGCCAGTAGCTCCTATGGCCTGGGGAGGGGCACGCCTTC520.10098852226602707No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)