FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003246273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181940
Sequences flagged as poor quality0
Sequence length300
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG49202.7041881939100803No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC40312.215565571067385No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA39792.186984720237441No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA33821.8588545674398154No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGC33591.8462130372650325No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG31641.7390348466527428No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGCCTGGGTGGGA31031.7055073101022316No Hit
TGTAAAACGACGGCCAGTCAAGCAGACAGAGAGCTGAAGGAGTGCCAAGG29531.6230625480927778No Hit
TGTAAAACGACGGCCAGTCCAGAGATCAATAATAGCTCAGGGGAGATGGA28671.5757942178740245No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT28651.5746949543805648No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC26231.4416840716719799No Hit
TGTAAAACGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT25061.3773771573046059No Hit
TGTAAAACGACGGCCAGTGGCTCCCCAGCTGTCTCTCTTGTAAAACGACG24251.332856985819501No Hit
TGTAAAACGACGGCCAGTAGTCAGCCACGTTGGAACCAGTACTCACGCAA22711.2482136968231285No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG21721.193800153896889No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG21441.1784104649884577No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGCCCCAATCCTGTCTC21161.1630207760800264No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT20931.1503792459052435No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGTGCTGCTGAATG20201.110256128393976No Hit
TGTAAAACGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA19851.0910190172584369No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCAGCAGGATGGAG18921.0399032648125754No Hit
TGTAAAACGACGGCCAGTGCCACTTCTGAAGGAGGTTCCCCCAGCAGTGC18030.9909860393536332No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC16610.9129383313180169No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCACATGCCTCCC15830.870067055073101No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGGTTGGAGGTG14810.8140046169066726No Hit
TGTAAAACGACGGCCAGTTGTCTGGTGGTGGAAGGAGGTGCACGCCACCA14780.8123557216664835No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGGTGCTCTGGT14700.8079586676926459No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCCAAGAGGTGGGG14510.7975156645047818No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGCGTGGGGCACTC14330.7876222930636473No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC14200.7804770803561614No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC14000.7694844454215676No Hit
TGTAAAACGACGGCCAGTAAGAGGTGAGCGAGGTCTGCGCCACGTGATCC13320.7321094866439486No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC12780.7024293723205453No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATCACATTTCAGCA12660.695833791359789No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCATAAACACAAA12560.6903374738924921No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCAGTTCACT12500.6870396834121139No Hit
TGTAAAACGACGGCCAGTCCTGTGCCCCAGCCTCCTTAATGTGTGCACTC11500.6320765087391448No Hit
TGTAAAACGACGGCCAGTTCCATTACTCTACACAAGCAGGGTGGGGTGCT11490.6315268769924152No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATCGCATTTCAGCA11380.6254809277783885No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA11240.6177860833241727No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG11130.6117401341101462No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAGGCACGCAGGCT10510.5776629658129053No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG10310.5666703308783115No Hit
TGTAAAACGACGGCCAGTCCCTGTCCTGAGCACCGTTGCTAACCCCCAGT10220.5617236451577443No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG10220.5617236451577443No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG9800.5386391117950973No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGTGTCGTGGGGCA9490.5216005276464769No Hit
TGTAAAACGACGGCCAGTACCTCATGTCACGTGCGTCCACAGTGACCTGT9410.5172034736726393No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG9270.5095086292184237No Hit
TGTAAAACGACGGCCAGTTTGACCGTCCCACCTGCCTTGGTTTTCCCTTT9210.5062108387380455No Hit
TGTAAAACGACGGCCAGTGGTGTTACCGTGGTCACCCGCAGATTTGGGGC9160.5034626800043971No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCAAGAGGTGGGGT8940.49137078157634384No Hit
TGTAAAACGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT8640.47488182917445315No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG8490.4666373529735078No Hit
TGTAAAACGACGGCCAGTGCCAATCCTGAGGCCATCAACACAAGGACTGG8480.46608772122677805No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGTTCGGAGGCAGG8460.46498845773331865No Hit
TGTAAAACGACGGCCAGTAGGTGAAGAAGAAAAGACAAAGACCTACAGTG8310.45674398153237333No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCCTGAGG7800.428712762449159No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGGCTTCTGGGCTT7730.4248653402220512No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC7720.4243157084753216No Hit
TGTAAAACGACGGCCAGTTGCCCATCAGCAATCCTGCCCACACAAAAAGT7590.4171704957678356No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT7560.4155216005276465No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG7330.40288007035286355No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAAGCCCCAGACCC7240.3979333846322964No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGGTCTGTGCGG7240.3979333846322964No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGTCCCTGTTTTACAATGCA7170.39408596240518856No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC7160.39353633065845883No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACACTGCCGAGTG7040.38694074969770254No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGTGTCAGGGGGCTGCT6960.382543695723865No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTACCCAGTGGTGTG6710.36880290205562277No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAGGACGGGTGGCC6520.3583598988677586No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG6190.3402220512256788No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA6120.33637462899857096No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGGACCGGGTCGGT5490.3017478289546004No Hit
TGTAAAACGACGGCCAGTTCTTTGGCTCTACCTAAAAGTTGGTCATAGCT5380.2957018797405738No Hit
TGTAAAACGACGGCCAGTCCTCACTCCCCACCCAGGATGTAAAACGACGG5240.2880070352863581No Hit
TGTAAAACGACGGCCAGTCCCTGTCCTGAGCACCGTTGGCTCCAGGATCG5230.28745740353962845No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCAGGGCTTGAACCC5210.28635814004616905No Hit
TGTAAAACGACGGCCAGTAGCAGGCAGGTCCTTCCAGACAGCCAATGAGG5190.28525887655270965No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCTGCAGACAGGAT5190.28525887655270965No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA5180.28470924480598003No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCATGGCCCCTGC5040.27701440035176433No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCATCAGGTTCCC4960.2726173463779268No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCTACTTCAATGC4790.263273606683522No Hit
TGTAAAACGACGGCCAGTCAAGCAGACAGAGAGCTGAAGGAGGAGGACCC4650.25557876222930637No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGAGCTCAAGGGT4600.2528306034956579No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGCTGGGAACTGTGAG4590.2522809717489282No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT4520.2484335495218204No Hit
TGTAAAACGACGGCCAGTTGTCTGGTGGTGGAAGGAGGCAGCATCGCCAC4420.24293723205452347No Hit
TGTAAAACGACGGCCAGTGGTGTTACCGTGGTCACCCGCAGGGACACGGA4340.23854017808068595No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGGTGGCCCTTC4330.23799054633395622No Hit
TGTAAAACGACGGCCAGTTGTCTGGTGGTGGAAGGAGGACCTAGTCCACG4300.23634165109376717No Hit
TGTAAAACGACGGCCAGTTGGAGTGCCTGGGTTAGAACCCAGGCATTCAA4300.23634165109376717No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCACACCAGCCCC4250.23359349236011873No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCGCCTCGGCCTCC4180.22974607013301088No Hit
TGTAAAACGACGGCCAGTTGTGGACCACAGTTTGCAAGTGTAAAACGACG4180.22974607013301088No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCGGCTCACT4070.22370012091898428No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG4060.2231504891722546No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGTGTTCGGAGGCA3940.21655490821149828No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCAGGGCTTGGACCC3930.21600527646476858No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGAAGCCCAGTCAGG3860.2121578542376608No Hit
TGTAAAACGACGGCCAGTTCTTTGGCTCTACCTAAAAGTTGGTCAGCAAA3810.2094096955040123No Hit
TGTAAAACGACGGCCAGTTCTTCACCTCCCAAAATGCTGGGATTACAGGC3770.20721116851709354No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG3700.2033637462899857No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTTGTCTCACT3600.1978674288226888No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCCAGGATGGAGGG3540.19456963834231064No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTTGGCTCACT3410.18742442563482467No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGTGACGCAGGTTC3370.18522589864790592No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGCTCTGGTG3310.18192810816752775No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGGGGAGGATCCTC3290.18082884467406837No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGACCTGGCGCAGGC3220.17698142244696055No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG3130.1720347367263933No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCACTGCCTAGCAGG3050.16763768275255578No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT2920.1604924700450698No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCACGGTCACTG2900.15939320655161043No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC2780.15279762559085414No Hit
TGTAAAACGACGGCCAGTTGAAGAATGGTGGCTTGTTTCATGGTGGCCTT2710.1489502033637463No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGATATCTCCTGCAC2660.14620204463009784No Hit
TGTAAAACGACGGCCAGTGGCTCTATATCAGGGCCCGCCGGTCATAGCTG2640.14510278113663846No Hit
TGTAAAACGACGGCCAGTCTGGGCCTTGGCTTCTTCCTCAATCATCTAGG2590.14235462240299002No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGTGAGCGAAG2560.14070572716280094No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCAGGAAATCAGGCT2460.13520940969550402No Hit
TGTAAAACGACGGCCAGTTGGAGTGCCTGGGTTAGAACCCTGCTGGTACC2420.13301088270858524No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCAGGGCTTGGGCCC2370.1302627239749368No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCTCCTTCCCAT2310.12696493349455865No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA2260.1242167747609102No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCGAGCAGGTGGC2240.12311751126745081No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCATGGCTCCAC2220.12201824777399142No Hit
TGTAAAACGACGGCCAGTGGTGTTACCGTGGTCACCCGCGCAAACGTCGG2220.12201824777399142No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCAGGATGGAGGGG2210.12146861602726174No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGAGGCTCCTTCC2210.12146861602726174No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCATAAACGCAAG2210.12146861602726174No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCCCGGCTCACC2200.12091898428053204No Hit
TGTAAAACGACGGCCAGTGCCATCGGCTCCCAAGTTTTATGTCCTAGAGC2150.11817082554688359No Hit
TGTAAAACGACGGCCAGTGGTCCCACTCTGGCTTTCCCTGAGCGAGATAA2140.1176211938001539No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGGCCCTCGTTCCC2140.1176211938001539No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGACAGCAGCTCTGG2130.1170715620534242No Hit
TGTAAAACGACGGCCAGTTCCATTACTCTACACAAGCAGGGTACACTTAG2080.11432340331977577No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCAGTGGTGCGATGGTCATAGCTG1990.10937671759920853No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCAGTGGTGCGATG1940.10662855886556008No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCACGCATCTCCAGC1940.10662855886556008No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCAGCTCACT1940.10662855886556008No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCGTGACCAAGAGG1910.104979663625371No Hit
TGTAAAACGACGGCCAGTGGGAGGCCATGTTGAACTTATTCAGAATGTAC1890.10388040013191162No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACCGACGGCCAGT1880.10333076838518193No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGGTGTCCTGCGTG1870.10278113663845223No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG1860.10223150489172256No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATTTCAGTCCACT1830.10058260965153348No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTTGGCTAACT1820.10003297790480378No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)