FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161798
Sequences flagged as poor quality0
Sequence length300
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG31681.9579970086156813No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT28241.7453862223266048No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT23711.4654074834052337No Hit
CAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC19741.220039802716968No Hit
CAGGAAANAGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA19721.2188036934943571No Hit
CAGGAAANAGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT15950.9857971050322007No Hit
CAGGAAANAGCTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC15160.9369707907390697No Hit
CAGGAAANAGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT14920.9221374800677388No Hit
CAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT14310.8844361487781061No Hit
CAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC13730.8485889813223896No Hit
CAGGAAANAGCTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC13460.8319015068171424No Hit
CAGGAAANAGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC12160.7515544073474333No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC12040.7441377520117678No Hit
CAGGAAANAGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA11580.7157072398917168No Hit
CAGGAAANAGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT10910.6742975809342513No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA10780.6662628709872804No Hit
CAGGAAANAGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT10780.6662628709872804No Hit
CAGGAAANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG10460.6464851234255059No Hit
CAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT9850.6087837921358731No Hit
CAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG9580.5920963176306259No Hit
CAGGAAANNGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA8420.5204019827191931No Hit
CAGGAAANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC7690.4752839960938948No Hit
CAGGAAANAGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT7310.45179792086428755No Hit
CAGGAAANAGCTATGANNCCGTAGCCACGCCCCTATTGCAGGAAACAGCT6620.4091521526842111No Hit
CAGGAAANAGCTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA6470.3998813335146294No Hit
CAGGAAANNGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT6410.3961730058467966No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA6220.384429968231993No Hit
CAGGAAANAGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG5930.36650638450413475No Hit
CAGGAAANAGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT5900.3646522206702184No Hit
CAGGAAANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC5790.3578536199458584No Hit
CAGGAAANAGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC5570.3442564184971384No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT5510.3405480908293057No Hit
CAGGAAANNGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT5420.33498559932755656No Hit
CAGGAAANNGCTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC5230.32324256171275295No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT5210.32200645249014204No Hit
CAGGAAANAGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC5070.3133536879318657No Hit
CAGGAAANAGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG5010.30964536026403294No Hit
CAGGAAANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA4960.3065550872075056No Hit
CAGGAAANAGCTATGANNCAGGGTGACTCCGATCCTGGAGGACAAGGCGG4830.29852037726053476No Hit
CAGGAAANAGCTATGANNAGAGACCAGATGCATAAGCACAGCAGCATTCC4820.2979023226492293No Hit
CAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC4810.29728426803792385No Hit
CAGGAAANNGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC4660.288013448868342No Hit
CAGGAAANNGCTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC4580.28306901197789835No Hit
CAGGAAANAGCTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA4330.267617646695262No Hit
CAGGAAANNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT4120.2546384998578474No Hit
CAGGAAANNGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA4070.25154822680132016No Hit
CAGGAAANNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG3990.24660378991087653No Hit
CAGGAAANNGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT3880.2398051891865165No Hit
CAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC3680.2274440969604074No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG3680.2274440969604074No Hit
NAGGAAANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG3490.21570105934560377No Hit
CAGGAAANNGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT3470.21446495012299288No Hit
CAGGAAANAGCTATGANNCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC3410.21075662245516014No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA3390.20952051323254922No Hit
CAGGAAANAGCTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA3290.20333996711949467No Hit
CAGGAAANAGCTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC3180.19654136639513467No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC3180.19654136639513467No Hit
NAGGANANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG3140.19406914794991287No Hit
NAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3060.18912471105946924No Hit
NAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2980.18418027416902558No Hit
CAGGANANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG2930.1810900011124983No Hit
CAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2900.17923583727858194No Hit
CAGGAAANAGCTATGANNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT2820.17429140038813828No Hit
CAGGAAANAGCTATGANNGGTAGCAACGTCCCACCCAGCTTCTTCCTCCT2630.16254836277333465No Hit
CAGGAAANNGCTATGANNCCGTAGCCACGCCCCTATTGCAGGAAACAGCT2580.15945808971680736No Hit
CAGGAAACNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2570.15884003510550193No Hit
CAGGAAANAGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT2550.15760392588289102No Hit
CAGGAAANAGCTATGANNCCGTAGCCACACCCCTTAAGCCAGAGCCGACT2520.15574976204897464No Hit
CAGGAAANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA2510.1551317074376692No Hit
CAGGAAANAGCTATGANNCTGGCCTCCTTCAATGCATTTAATGGCCTTAT2500.15451365282636373No Hit
CAGGAAACAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2470.15265948899244738No Hit
CAGGAAANNGCTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA2420.1495692159359201No Hit
CAGGAAANAGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT2410.14895116132461464No Hit
NAGGANANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2390.14771505210200375No Hit
CAGGAAANNGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT2360.14586088826808735No Hit
CAGGAAANAGCTATGANNACCTCTCTGGGCCTGTCTCCTCATCTTGATCT2320.14338866982286555No Hit
CAGGAAANAGCTATGANNGCTGCTGACCCAACCCTACCCTGTCCAGCCCC2320.14338866982286555No Hit
CAGGAANNNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2280.14091645137764372No Hit
NAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2280.14091645137764372No Hit
CAGGAAANAGCTATGANNAGGTCCAGATGGCTGGTGACTCTGACTCTGTC2250.13906228754372738No Hit
NAGGANANNGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA2230.13782617832111646No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG2200.1359720144872001No Hit
CAGGAAANAGCTATGANNGGTAGCAACGTCCCACCCAGCTAGCCCCTGAT2180.13473590526458917No Hit
NAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2150.13288174143067283No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC2130.1316456322080619No Hit
CAGGAAANNGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG2120.13102757759675646No Hit
CAGGANANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2110.130409522985451No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC2110.130409522985451No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC2100.12979146837414554No Hit
CAGGAAANAGCTATGANNGGTAGCAACGTCCCACCCAGCAGCCCCTTCCC2090.12917341376284008No Hit
CAGGAAANAGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA2060.1273192499289237No Hit
CAGGAAANNGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC2050.12670119531761828No Hit
CAGGAAANAGCTATGANNGGAGGCTGAGGTGGGAGGATCGCTTGATCCCG2050.12670119531761828No Hit
CAGGAAANAGCTATGANNATATGACAACTTGAACACATCTGACACTTCCT2040.1260831407063128No Hit
CAGGANANNGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1990.12299286764978552No Hit
NAGGAAANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1960.12113870381586916No Hit
NAGGAAANAGCTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG1960.12113870381586916No Hit
CAGGAAANAGCTATGANNCCCAAACCTGCGGAGGCACGGGGATGGCAGTG1950.12052064920456372No Hit
CAGGAAANNGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG1940.11990259459325825No Hit
CAGGANANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1930.1192845399819528No Hit
CAGGAAANNGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT1910.11804843075934189No Hit
NAGGAAANNGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1890.11681232153673098No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCCAAACCAG1880.11619426692542553No Hit
CAGGAAANAGCTATGANNTGCCCAGTTGTTTCCAGAACCTTGTTTTTGAC1880.11619426692542553No Hit
CAGGAAANNGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT1860.11495815770281462No Hit
CAGGAAANNGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC1840.1137220484802037No Hit
CAGGAAANNGCTATGANNCAGGGTGACTCCGATCCTGGAGGACAAGGCGG1830.11310399386889826No Hit
CAGGAAANAGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG1790.11063177542367643No Hit
CAGGAAANAGCTATGANNGCCCTGGCCACTGTGGTTAACTGGCCGTCGTT1790.11063177542367643No Hit
CAGGAAANAGCTATGANNCTGGCCTCCTTCAATGCATTTAATGGCCTTCT1740.10754150236714916No Hit
CAGGAAACAGCNATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1720.10630539314453824No Hit
CAGGAAACAGCTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG1720.10630539314453824No Hit
CAGGAAACAGCTATGANCTGTTTTGCCCCTGAAATGAATATTCATCGTCT1720.10630539314453824No Hit
CAGGAAACNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1710.1056873385332328No Hit
CAGGAAANNGCTATGANNAGAGACCAGATGCATAAGCACAGCAGCATTCC1700.10506928392192735No Hit
CAGGAAACNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1690.10445122931062188No Hit
CAGGAAANNGCTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA1670.10321512008801097No Hit
CAGGAAANAGCTATGANNAAGTTGGTCAGCCCTGGTGACTCATTGGATAT1660.10259706547670552No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)