FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82432
Sequences flagged as poor quality0
Sequence length300
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC16732.0295516304347827No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGGTTGGAGGTG12021.4581715838509317No Hit
TGTAAAACGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG11641.4120729813664596No Hit
TGTAAAACGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT11081.344138198757764No Hit
TGTAAAACGACGGCCAGTTCCCAAAGGCTGGTGGAGGAGATGCTGAAGAT10781.3077445652173914No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA9791.1876455745341614No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC9641.1694487577639752No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGTGGGAGCCCC9571.1609569099378882No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG9491.151251940993789No Hit
TGTAAAACGACGGCCAGTAGGTCATCCTCCTGCCTCGGGTCAGTGTTCCT9221.1184976708074534No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC8921.0821040372670807No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG8891.0784646739130435No Hit
TGTAAAACGACGGCCAGTTGAAGAATGGTGGCTTGTTTCATGGTGGCCTT8721.0578416149068322No Hit
TGTAAAACGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT8261.0020380434782608No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCTCCTTCCCAT7800.9462344720496894No Hit
TGTAAAACGACGGCCAGTTGCCCAACGTTGTAGTGTTGAGTGCATGGCAT7640.9268245341614906No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA7480.907414596273292No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCATTGTGGGCTA7200.873447204968944No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG6950.8431191770186336No Hit
TGTAAAACGACGGCCAGTCCTCTGGCATGCTGGTAGACTTGTGCCTGTTA6830.8285617236024844No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG6610.8018730590062112No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG6600.8006599378881988No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT6530.7921680900621118No Hit
TGTAAAACGACGGCCAGTACATGCGTGGCCCTGTCTACTCATCCCCCTCT6280.7618400621118012No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCTGGAGTGCAGTG5740.6963315217391304No Hit
TGTAAAANGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC5320.6453804347826086No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA5250.6368885869565217No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC4960.6017080745341615No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT4960.6017080745341615No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT4800.5822981366459627No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTGGGGTTAGGCAT4610.5592488354037267No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTCCATTTGGTGTG4530.5495438664596273No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC4530.5495438664596273No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG4230.5131502329192547No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGAGCTCAAGGGT4060.4925271739130435No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC4020.4876746894409938No Hit
TGTAAAANGACGGCCAGTTCCCAAAGGCTGGTGGAGGAGATGCTGAAGAT4000.4852484472049689No Hit
TGTAAAANGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGGTTGGAGGTG3930.476756599378882No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG3880.4706909937888199No Hit
TGTAAAANGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT3850.4670516304347826No Hit
TGTAAAACGACGGCCAGTACCGACTGGAGAGGAAGGCACTGGTGGTAGCT3760.4561335403726708No Hit
TGTAAAANGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG3560.4318711180124224No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC3550.4306579968944099No Hit
TGTAAAACGACGGCCAGTTGGGGTCTCCTGGTCCCATTCAGGGCCATGTG3480.422166149068323No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGGTCTGTGCGG3480.422166149068323No Hit
TGTAAAACGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT3450.4185267857142857No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC3430.4161005434782609No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC3410.41367430124223603No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG3350.4063955745341615No Hit
TGTAAAANGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG3240.39305124223602483No Hit
TGTAAAANGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT3160.38334627329192544No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT3140.3809200310559006No Hit
TGTAAAANGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA3140.3809200310559006No Hit
TGTAAAANGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC3060.3712150621118013No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTACCCAGTGGTGTG3040.3687888198757764No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCAGGGTCCTAAA3020.36636257763975155No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGACCTGGCGCAGGC3010.36514945652173914No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTCCCGACGCCAGG3010.36514945652173914No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCGTGGGTC2970.36029697204968947No Hit
TGTAAAANGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA2930.35544448757763975No Hit
TGTAAAANGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG2900.3518051242236025No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC2810.3408870341614907No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT2780.3372476708074534No Hit
TGTAAAANGACGGCCAGTAGGTCATCCTCCTGCCTCGGGTCAGTGTTCCT2770.336034549689441No Hit
TGTAAAANGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGTGGGAGCCCC2760.33482142857142855No Hit
TGTAAAACGACGGCCAGTTCCTTCTTGGGTGGGTATTTTGCAACTGGGTA2740.33239518633540377No Hit
TGTAAAANGACGGCCAGTTGAAGAATGGTGGCTTGTTTCATGGTGGCCTT2700.32754270186335405No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG2610.31662461180124224No Hit
TGTAAAANGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC2600.3154114906832298No Hit
TGTAAAACGACGGCCAGTACCGACTGGAGAGGAAGGCAGTGCCTTGCCAG2530.30691964285714285No Hit
TGTAAAANGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCATTGTGGGCTA2520.30570652173913043No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGCACATGGGT2470.2996409161490683No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCCCCCATGAACGG2460.2984277950310559No Hit
TGTAAAANGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCTCCTTCCCAT2460.2984277950310559No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG2390.28993594720496896No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGCCTGGGTGGGA2360.2862965838509317No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAGGATGCTGAGTGGGAG2340.2838703416149069No Hit
TGTAAAANGACGGCCAGTTGCCCAACGTTGTAGTGTTGAGTGCATGGCAT2330.2826572204968944No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTGACCCTTCCAGGA2310.28023097826086957No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC2300.27901785714285715No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTGTAAAACGACGG2300.27901785714285715No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGAAGCCCAGTCAGG2270.2753784937888199No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC2250.27295225155279507No Hit
TGTAAAANGACGGCCAGTCCTCTGGCATGCTGGTAGACTTGTGCCTGTTA2230.2705260093167702No Hit
TGTAAAANGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG2230.2705260093167702No Hit
TGTAAAANGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA2120.25718167701863354No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGAGCTCAACCGT2120.25718167701863354No Hit
TGTAAAANGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG2120.25718167701863354No Hit
TGTAAAANGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT2090.25354231366459623No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTCTAGCCTGGGCA2040.24747670807453415No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACACTGCCGAGTG2030.24626358695652176No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG1990.24141110248447206No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG1970.2389848602484472No Hit
TGTAAAANGACGGCCAGTGTCCCAGGTGTATGGAGCGCTGGAGTGCAGTG1960.2377717391304348No Hit
TGTAAAANGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG1960.2377717391304348No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCCCTCGGAGCTG1940.23534549689440995No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGGTCTGGGTG1890.22927989130434784No Hit
TGTAAAACGACGGCCAGTAGGTGAAGAAGAAAAGACAAAGACCTACAGTG1860.22564052795031056No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCAGTGGTGCGATG1830.2220011645962733No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGGGGGTGCCTCAA1800.218361801242236No Hit
TGTAAAANGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT1760.21350931677018634No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGAGGCTCCTTCC1700.2062305900621118No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG1660.2013781055900621No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCTGCTGTCCTCA1640.19895186335403725No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTTCAGCCCAGGAG1620.19652562111801242No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT1600.19409937888198758No Hit
TGTAAAANGACGGCCAGTACATGCGTGGCCCTGTCTACTCATCCCCCTCT1590.19288625776397514No Hit
TGTAAAANGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT1550.18803377329192547No Hit
TGTAAAANGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC1510.18318128881987578No Hit
TGTAAAACGACGGCCAGTTGGGGTCTCCTGGTCCCATTCGAGCATGCTGC1510.18318128881987578No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGCTTTGCCAACAATG1490.18075504658385091No Hit
TGTAAAANGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG1460.17711568322981366No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA1460.17711568322981366No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTGGGTGGGGTTAG1460.17711568322981366No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTTCAGCCCAGGGG1420.17226319875776397No Hit
TGTAAAANGACGGCCAGTCAGCAGCAACTCCCTGTGCTGGGGTTAGGCAT1400.16983695652173914No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGACCAGACG1390.1686238354037267No Hit
TGTAAAACGACGGCCAGTAGGTCATCCTCCTGCCTCGGCCTCCCAAAGTG1380.16741071428571427No Hit
TGTAAAANGACGGCCAGTACCGACTGGAGAGGAAGGCACTGGTGGTAGCT1380.16741071428571427No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTCACGCCTGTAAT1380.16741071428571427No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGTCCCAGCTACT1350.16377135093167702No Hit
TGTAAAANGACGGCCAGTGTGGTAACTGCTGCAGGGCTCCATTTGGTGTG1340.1625582298136646No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGAGCTCAAGGGC1310.15891886645962733No Hit
TGTAAAANGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG1300.1577057453416149No Hit
TGTAAAANGACGGCCAGTGGTACGTGGTGGTGGCTGAGGAGCTCAAGGGT1290.15649262422360247No Hit
TGTAAAANGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC1270.15406638198757766No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC1240.15042701863354038No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTGATAAGGAAGGA1240.15042701863354038No Hit
TGTAAAANGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC1210.1467876552795031No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGCACTGCTCT1200.1455745341614907No Hit
TGTAAAANGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC1200.1455745341614907No Hit
TGTAAAANGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC1170.14193517080745344No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGGTGGCCCTTC1150.13950892857142858No Hit
TGTAAAANGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC1140.13829580745341616No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCACCCCGGGAGC1130.13708268633540371No Hit
TGTAAAANGACGGCCAGTTGGGGTCTCCTGGTCCCATTCAGGGCCATGTG1120.1358695652173913No Hit
TGTAAAACGACGGCCAGTGCCCAAGGTTGGTGAGATGGGAACCACCCAGC1110.13465644409937888No Hit
TGTAAAANGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGGTCTGTGCGG1110.13465644409937888No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGTGTAAAACGACG1100.13344332298136646No Hit
TGTAAAANGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT1090.13223020186335402No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGCACATGGTT1050.12737771739130435No Hit
TGTAAAACGACGGCCAGTTCCTGGGCTTCTCTTGAGTACGGAGGAAAGTG1050.12737771739130435No Hit
TGTAAAANGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCAGGGTCCTAAA1040.12616459627329193No Hit
TGTAAAANGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT1020.12373835403726707No Hit
TGTAAAACGACGGCCAGTTCTTCACCTCCCAAAATGCTGGGATTACAGGC1010.12252523291925466No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTCCCTTCTCTTTG970.11767274844720498No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGCTGAGGGAGGAT960.11645962732919254No Hit
TGTAAAACGACGGCCAGTAGTGGAAGCTATTAGCACCAATGACAACTCCC950.11524650621118013No Hit
TGTAAAANGACGGCCAGTACACATTGAGAGCATCAGTGGGGCACATGGGT950.11524650621118013No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGGCTGCCCCGGGA940.1140333850931677No Hit
TGTAAAANGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC930.11282026397515528No Hit
TGTAAAANGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCGTGGGTC930.11282026397515528No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTCCATTGCACTCC910.11039402173913043No Hit
TGTAAAANGACGGCCAGTGAGGACGAGAGCGAGGAGGACCTGGCGCAGGC910.11039402173913043No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAAGCCCCAGACCC900.109180900621118No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGACGTGCTGGTGAA900.109180900621118No Hit
TGTAAAANGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG890.1079677795031056No Hit
TGTAAAANGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG890.1079677795031056No Hit
TGTAAAANGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG880.10675465838509317No Hit
TGTAAAACGACGGCCAGTCCCGGGCGTCAGAGTGAGAACTGTAAAACGAC870.10554153726708075No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGCGCCTG870.10554153726708075No Hit
TGTAAAANGACGGCCAGTAGGTGAAGAAGAAAAGACAAAGACCTACAGTG860.10432841614906832No Hit
TGTAAAANGACGGCCAGTACCGGGCCAGGTCAGTTACTCCCGACGCCAGG860.10432841614906832No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTCACCATTTGGTG850.1031152950310559No Hit
TGTAAAANGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC840.10190217391304347No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)