FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003246302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137044
Sequences flagged as poor quality0
Sequence length300
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT14531.0602434254691924No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT13881.0128134029946587No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGCACTCCTTC12360.9019001196695952No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCTGAGGCAGGAGA11270.8223636204430694No Hit
CAGGAAACAGCTATGACCGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC10410.759610052245994No Hit
CAGGAAACAGCTATGACCAGCGTCTCATTCCATAAAGGCCACCATGAAAC10110.737719272642363No Hit
CAGGAAACAGCTATGACCTCCTGCCCCACCCTGATACAGGTCACTGTGGA8930.6516155395347479No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCTCTGCAGCTAGC8880.6479670762674762No Hit
CAGGAAACAGCTATGACCGTCTCAGGGATCACGTGGCGCAGACCTCGCTC8830.6443186130002043No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT8550.6238872187034821No Hit
CAGGAAACAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG8500.6202387554362102No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC8160.5954292052187619No Hit
CAGGAAACAGCTATGACCTGACAGCACAAGGAACACTGACCCGAGGCAGG7850.5728087329616766No Hit
CAGGAAANAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT7640.5574851872391349No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCCGTCCTCGGAC7360.5370537929424127No Hit
CAGGAAANAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT7280.5312162517147777No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG6990.5100551647646011No Hit
CAGGAAACAGCTATGACCTGGAAACCCATGCCATGCACTCAACACTACAA6650.4852456145471527No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA6570.47940807331951785No Hit
CAGGAAACAGCTATGACCGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG6320.4611657569831587No Hit
CAGGAAACAGCTATGACCGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA6220.45386883044861503No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC6190.451679752488252No Hit
CAGGAAANAGCTATGACCGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC5910.4312483581915297No Hit
CAGGAAANAGCTATGACCTGGAGTTTGAAACAGCACCTTGGCACTCCTTC5900.4305186655380754No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT5450.39768249613262896No Hit
CAGGAAANAGCTATGACCAATGCAAGGGACCACCAGGCTGAGGCAGGAGA5400.3940340328653571No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC5330.3889261842911766No Hit
NAGGAAANAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT5330.3889261842911766No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA5300.38673710633081343No Hit
NAGGAAANAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT5220.3808995651031785No Hit
CAGGAAANAGCTATGACCAGCGTCTCATTCCATAAAGGCCACCATGAAAC5160.37652140918245236No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAAGGCCGCAGCTCC5030.3670354046875456No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACGGAGATCCAGCC4890.3568197075391845No Hit
CAGGAAANAGCTATGACCCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT4890.3568197075391845No Hit
NAGGAAANAGCTATGACCTGGAGTTTGAAACAGCACCTTGGCACTCCTTC4860.3546306295788214No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCTGGGTGCTGGTG4750.3466040103908234No Hit
CAGGAAANAGCTATGACCGCTGCTGACCCAACCCTACCTCTGCAGCTAGC4670.3407664691631884No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCACAGGAAACAGC4580.33419923528209916No Hit
CAGGAAANAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG4560.33273984997519046No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCAGGAAACAGCT4510.3290913867079186No Hit
CAGGAAANAGCTATGACCTCCTGCCCCACCCTGATACAGGTCACTGTGGA4460.3254429234406468No Hit
CAGGAAANAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC4410.32179446017337493No Hit
CAGGAAANAGCTATGACCTGACAGCACAAGGAACACTGACCCGAGGCAGG4160.30355214383701584No Hit
CAGGAAANAGCTATGACCGTCTCAGGGATCACGTGGCGCAGACCTCGCTC4090.2984442952628353No Hit
NAGGAAANAGCTATGACCGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC4070.2969849099559265No Hit
CAGGAAACAGCTATGACCATCGCACCACTGCACTCCAGCGCTCCATACAC4010.29260675403520037No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC3810.2780129009661131No Hit
CAGGAAANAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA3790.27655351565920433No Hit
CAGGAAANAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG3760.2743644376988413No Hit
NAGGAAANAGCTATGACCAATGCAAGGGACCACCAGGCTGAGGCAGGAGA3730.2721753597384781No Hit
CAGGAAANAGCTATGACCTAGAGGACACCCAAGGCCACCCGTCCTCGGAC3690.2692565891246607No Hit
NAGGAAANAGCTATGACCAGCGTCTCATTCCATAAAGGCCACCATGAAAC3690.2692565891246607No Hit
CAGGAAANAGCTATGACCTGGAAACCCATGCCATGCACTCAACACTACAA3540.2583111993228452No Hit
CAGGAAANAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC3480.25393304340211903No Hit
CAGGAAACAGCTATGACCTGACTGAAAGGCTTTTCCACGTGCACTGCTGG3440.2510142727883016No Hit
CAGGAAANAGCTATGACCGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG3430.2502845801348472No Hit
NAGGAAANAGCTATGACCCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT3320.2422579609468492No Hit
NAGGAAANAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG3300.24079857563994045No Hit
NAGGAAANAGCTATGACCTGACAGCACAAGGAACACTGACCCGAGGCAGG3270.23860949767957737No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGTCTGCAGCCTGG3260.23787980502612302No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG3210.23423134175885119No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT3190.23277195645194246No Hit
CAGGAAANAGCTATGACCGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA3170.2313125711450337No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG3160.23058287849157935No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT3150.22985318583812497No Hit
NAGGAAANAGCTATGACCGTCTCAGGGATCACGTGGCGCAGACCTCGCTC3120.22766410787776187No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCAGGAAACAGCTA3110.22693441522430752No Hit
NAGGAAANAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC3080.22474533726394444No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAACGCAGCTCCCCA3070.22401564461049006No Hit
CAGGAAANAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA3060.2232859519570357No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCAGCACAGGGAGT3060.2232859519570357No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCAGCAACAGGGAC3040.22182656665012696No Hit
CAGGAAANAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT3030.2210968739966726No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA3030.2210968739966726No Hit
NAGGAAANAGCTATGACCTCCTGCCCCACCCTGATACAGGTCACTGTGGA3020.22036718134321823No Hit
NAGGAAANAGCTATGACCGCTGCTGACCCAACCCTACCTCTGCAGCTAGC2990.21817810338285512No Hit
CAGGAAACAGCTATGACCACCTTTACCCAAATAGCTACCACCAGTGCCTT2940.21452964011558331No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGATACTACTA2930.21379994746212896No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCTGCCCAACACCC2910.2123405621552202No Hit
CAGGAAANAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2730.19920609439304163No Hit
NAGGAAANAGCTATGACCTAGAGGACACCCAAGGCCACCCGTCCTCGGAC2710.19774670908613295No Hit
NAGGAAANAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA2690.1962873237792242No Hit
CAGGAAACAGCTATGACCTGGCATCCAGGGCCTATTTGGTTCCTATTGAG2680.19555763112576982No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTAATTCA2650.19336855316540671No Hit
CAGGAAACAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG2650.19336855316540671No Hit
NAGGAAANAGCTATGACCTGGAAACCCATGCCATGCACTCAACACTACAA2600.1897200898981349No Hit
NAGGAAANAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG2590.18899039724468053No Hit
NAGGAAANAGCTATGACCGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG2590.18899039724468053No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGAGCCT2580.18826070459122618No Hit
NAGGAAANAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC2570.1875310119377718No Hit
CAGGAAANAGCTATGACCTCCTGGAACCTGCCTCCGAAGGCCGCAGCTCC2540.18534193397740872No Hit
CAGGAAANAGCTATGACCACAGGAGACAGGATTGGGGCTGGGTGCTGGTG2480.1809637780566825No Hit
CAGGAAANAGCTATGACCTAGAGGACACCCAAGGCCACGGAGATCCAGCC2460.1795043927497738No Hit
CAGGAAANAGCTATGACCATCGCACCACTGCACTCCAGCGCTCCATACAC2420.17658562213595633No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCCTCCCCACCTCC2420.17658562213595633No Hit
CAGGAAANAGCTATGACCAACACGGCAGTCAGAGGTGCCAGGAAACAGCT2410.17585592948250198No Hit
CAGGAAANAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG2380.17366685152213887No Hit
NAGGAAANAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA2350.1714777735617758No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT2310.1685590029479583No Hit
CAGGAAANAGCTATGACCCATGTGCCCCAGTAGAGAGCACAGGAAACAGC2290.16709961764104958No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT2270.16564023233414085No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGACAGGAAACAGCT2210.16126207641341467No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAGGCCCCTGTACC2200.16053238375996032No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCAAGCCTGACTG2170.1583433057995972No Hit
CAGGAAACAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG2150.15688392049268848No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGGTCCTCCTC2130.15542453518577976No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCACAGGGACGCAG2100.15323545722541665No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT2050.14958699395814484No Hit
CAGGAAACAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT2010.1466682233443274No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAAGGCAGAGGCCAG1990.14520883803741863No Hit
NAGGAAANAGCTATGACCGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA1970.1437494527305099No Hit
CAGGAAANAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC1950.14229006742360117No Hit
CAGGAAACAGCTATGACCTGCCCAGTTGTTTCCAGAACCTCAGGAAACAG1940.14156037477014682No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGTCTGGGTCCT1930.14083068211669245No Hit
NAGGAAANAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1900.13864160415632934No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGACAGGGCTGACTG1900.13864160415632934No Hit
CAGGAAANAGCTATGACCTGACTGAAAGGCTTTTCCACGTGCACTGCTGG1880.13718221884942064No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT1870.13645252619596626No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGGGGGCCTGGCT1860.13572283354251188No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGCCTGGCTTGTC1860.13572283354251188No Hit
CAGGAAACAGCTATGACCGATGTAGCAGTGCTCTGGAGGGTGCTTGTCCC1860.13572283354251188No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCTGCAGTGCGGT1820.13280406292869443No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAAGGCCCCATCCGG1810.13207437027524005No Hit
NAGGAAANAGCTATGACCTCCTGGAACCTGCCTCCGAAGGCCGCAGCTCC1800.1313446776217857No Hit
CAGGAAANAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA1770.1291555996614226No Hit
NAGGAAANAGCTATGACCACAGGAGACAGGATTGGGGCTGGGTGCTGGTG1770.1291555996614226No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC1770.1291555996614226No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCCCCGGGCAGA1760.12842590700796824No Hit
CAGGAAANAGCTATGACCAGGACCACCCCTCCATCCTGTCTGCAGCCTGG1740.12696652170105951No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAACAGGCCACCGCA1730.12623682904760514No Hit
NAGGAAANAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT1710.12477744374069642No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAACACGCAGCTCCC1710.12477744374069642No Hit
NAGGAAANAGCTATGACCTAGAGGACACCCAAGGCCACGGAGATCCAGCC1690.12331805843378768No Hit
CAGGAAANAGCTATGACCAACACGGCAGTCAGAGGTGCTGCCCAACACCC1690.12331805843378768No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG1690.12331805843378768No Hit
CAGGAAANAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT1680.12258836578033332No Hit
CAGGAAANAGCTATGACCTCCTGGAACCTGCCTCCGAACGCAGCTCCCCA1670.12185867312687897No Hit
CAGGAAACAGCTATGACCGATGCTCCTTCCTTGGCCCCAGGCCCTGGCGT1660.1211289804734246No Hit
NAGGAAANAGCTATGACCATCGCACCACTGCACTCCAGCGCTCCATACAC1660.1211289804734246No Hit
CAGGAAANAGCTATGACCAACACGGCAGTCAGAGGTGCAGGAAACAGCTA1640.11966959516651586No Hit
CAGGAAANAGCTATGACCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT1600.1167508245526984No Hit
CAGGAAANAGCTATGACCACCTTTACCCAAATAGCTACCACCAGTGCCTT1590.11602113189924403No Hit
CAGGAAANAGCTATGACCTGTTGTCTTTCAGGGGCCGCAGCAACAGGGAC1570.11456174659233531No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA1570.11456174659233531No Hit
NAGGAAANAGNTATGANCTGTTTTGCCCCTGAAATGAATATTCATCGTCT1560.11383205393888093No Hit
CAGGAAACAGCTATGACCGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG1550.11310236128542657No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT1550.11310236128542657No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTCCGGGACATTCT1550.11310236128542657No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTAGTTCC1510.11018359067160911No Hit
CAGGAAANAGCTATGACCTGCTTCAGTCGCCTGCACAAGGCCCCTGTACC1500.10945389801815476No Hit
CAGGAAANAGCTATGACCTGGAGTTTGAAACAGCACCTTGGATACTACTA1490.1087242053647004No Hit
CAGGAAANAGCTATGACCCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG1470.10726482005779166No Hit
NAGGAAANAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC1450.10580543475088293No Hit
NAGGAAANAGNTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1440.10507574209742856No Hit
NAGGAAANAGNTATGANCAATGCAAGGGACCACCAGGCTGAGGCAGGAGA1420.10361635679051982No Hit
NAGGAAANAGCTATGACCAACACGGCAGTCAGAGGTGCAGGAAACAGCTA1420.10361635679051982No Hit
NAGGAAANAGCTATGACCAACACGGCAGTCAGAGGTGCCAGGAAACAGCT1420.10361635679051982No Hit
CAGGAAANAGCTATGACCCCACCCTCATGCCTAACCCCAGCACAGGGAGT1420.10361635679051982No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCCAGGAAACAGCT1410.10288666413706547No Hit
CAGGAAANAGCTATGACCTGGCATCCAGGGCCTATTTGGTTCCTATTGAG1410.10288666413706547No Hit
CAGGAAACAGCTATGACCTGAAGTGGCTCCATCCTTCCATTTGTTTATAT1410.10288666413706547No Hit
CAGGAAANAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTAATTCA1400.10215697148361111No Hit
CAGGAAANAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG1400.10215697148361111No Hit
CAGGAAANAGCTATGACCGGGGATGGAGCAGGAAGTGACAGGAAACAGCT1390.10142727883015673No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG1390.10142727883015673No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)