FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103182
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG40183.894090054466864No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT30302.9365587020992034No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT25372.4587621872031944No Hit
TGTAAAACGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTCTGGCATGG23902.316295477893431No Hit
TGTAAAACGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT21632.0962958655579462No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCATTGTGGGCTA21062.0410536721521195No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG20441.9809656723071853No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA20281.9654590917020411No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG20231.9606132852629334No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGTGGGAGCCCC19581.8976178015545346No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC18931.8346223178461358No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA18441.7871334147428815No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG18231.7667810276986298No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG17441.6902172859607294No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC16501.5991161249055068No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG15931.54387393149968No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA14801.4343587059758485No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG13631.320966835300731No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT13211.280262061212227No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC12601.2211432226551142No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC11501.1145354809947472No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC11151.080614835920994No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC10611.0282801263786319No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG10280.9962978038805218No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC9060.8780601267662964No Hit
TGTAAAACGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA8620.8354170301021495No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC8580.8315403849508636No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC8220.7966505785892888No Hit
TGTAAAACGACGGCCAGTGTTCTACCGTCAGTCCTGGCATTATTTCTCTC7760.7520691593494989No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT7710.7472233529103913No Hit
TGTAAAACGACGGCCAGTCCAGAGATCAATAATAGCTCAGGGGAGATGGA7520.7288092884417825No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC7200.6977961272314939No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC6410.6212323854935938No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTGGTGGTCC6020.5834350952685546No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC5370.5204396115601558No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC4960.4807039987594735No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT4830.4681049020177938No Hit
TGTAAAACGACGGCCAGTAGTGACAACAGCCCCAAAGGAATGATCCCGCT4790.4642282568665077No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG4270.41383186989978876No Hit
TGTAAAACGACGGCCAGTTGGAGTGCCTGGGTTAGAACCCTGCTGGTACC4270.41383186989978876No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA3990.3866953538407862No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA3970.38475703126514316No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGCTACGGGGT3670.3556821926304976No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG3550.34405225717663934No Hit
TGTAAAACGACGGCCAGTCCTGCCGCCTGGTCTCACCTCGCCATGGTTCG3440.3333914830106026No Hit
TGTAAAACGACGGCCAGTCCTGCCGCCTGGTCTCACCTCGCTATGGTTCG3270.31691574111763676No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC3240.31400825725417225No Hit
TGTAAAACGACGGCCAGTCCTGTGCCCCAGCCTCCTTAATGTGTGCACTC3210.3111007733907077No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC3050.29559419278556337No Hit
TGTAAAACGACGGCCAGTCGCCTGAATAGGTGCAGGGGCACGAGCTCGGC3050.29559419278556337No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG3020.29268670892209886No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA2920.2829950960438836No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG2740.2655501928630963No Hit
TGTAAAACGACGGCCAGTTTGACCGTCCCACCTGCCTTGGTTTTCCCTTT2650.2568277412727026No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGTGAACGAAG2450.23744451551627221No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGCTCTGGTG2430.2355061929406292No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG2370.22969122521370006No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG2330.225814580062414No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC2250.21806128975984185No Hit
TGTAAAACGACGGCCAGTCGCCTGAATAGGTGCAGGGGCACGATATCCAT2250.21806128975984185No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA2220.21515380589637728No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCCGTGGG2200.21321548332073423No Hit
TGTAAAACGACGGCCAGTTGGGGTCTCCTGGTCCCATTCGAGCATGCTGC2090.20255470915469753No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGGCTTCTGGGCTT2000.19383225756430386No Hit
TGTAAAACGACGGCCAGTCAGTTCCCAGGGACTCTGCTCTATGAGAGAGA1940.18801728983737473No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC1830.17735651567133803No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC1800.17444903180787347No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA1760.1705723866565874No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAGCC1740.16863406408094433No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG1720.1666957415053013No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTTACAAGGTTTGT1670.16184993506619372No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC1660.1608807737783722No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG1650.15991161249055066No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC1590.15409664476362156No Hit
TGTAAAACGACGGCCAGTAGCCTTTCTACTCATTGGAACACTCAGGTGTA1400.1356825802950127No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCCT1320.12792928999244055No Hit
TGTAAAACGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA1310.126960128704619No Hit
TGTAAAACGACGGCCAGTCGCCTGAATAGGTGCAGGGGGACAGGGTCTTG1260.12211432226551143No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA1120.10854606423601017No Hit
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCTCAACAAGGAGACGATG1110.10757690294818864No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATGGCCGAGCAGGG1100.10660774166036711No Hit
TGTAAAACGACGGCTAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG1060.10273109650908105No Hit
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCCACAAGGAGACGATG1060.10273109650908105No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)