FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115988
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT47124.062489223023071No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC41953.6167534572541986No Hit
TGTAAAACGACGGCCAGTGAGCTGCTGACGCACTGTGACTCCAGCCAGAG40563.4969134738076355No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA40263.4710487291788805No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC38193.292581991240473No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG36623.157223161016657No Hit
TGTAAAACGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTCTGGCATGG33722.907197296272028No Hit
TGTAAAACGACGGCCAGTTCCATCCACTGCTGAGAAATCTCCAGGTCATA30432.6235472635100185No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG26942.322654067662172No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGTGGGAGCCCC24672.1269441666379283No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC23522.0277959788943685No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG23292.0079663413456563No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA21721.87260751112184No Hit
TGTAAAACGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG21661.867434562196089No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC20821.795013277235576No Hit
TGTAAAACGACGGCCAGTCCAGAGATCAATAATAGCTCAGGGGAGATGGA18281.5760251060454529No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC16901.4570472807531814No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC16871.4544608062903057No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC16761.4449770665930959No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC9490.8181880884229402No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC8960.7724937062454738No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG8850.7630099665482637No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC8620.7431803289995517No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT8610.7423181708452599No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG7610.6561023554160775No Hit
TGTAAAACGACGGCCAGTTGACACAGCTGCTTCTGAGTACAAGTATGTAG6570.5664379073697279No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACGGCCAGTGGCA5410.46642756147187636No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG5380.46384108700900095No Hit
TGTAAAACGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT5190.44746008207745624No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC5170.4457357657688727No Hit
TGTAAAACGACGGCCAGTCTCTCACCCACCTCCACCCATCAAAGGTGCCT5000.43107907714591165No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA4670.4026278580542815No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC4530.39055764389419595No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGACCATAGTTCACA4470.385384694968445No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT4460.38452253681415316No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG4390.3784874297341104No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA4270.3681415318826085No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG4250.3664172155740249No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC4010.3457254198710211No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC3950.3405524709452702No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGTCCCTGTTTTACAATGCA3150.27157981860192437No Hit
TGTAAAACGACGGCCAGTATCTCCTGGGGAGAAACGGCATCTGTGTCCAA3020.26037176259613065No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA3020.26037176259613065No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGTCCCTGTGTAAAACGACG2960.2551988136703797No Hit
TGTAAAACGACGGCCAGTAGCAGGCAGGTCCTTCCAGACAGCCAATGAGG2900.25002586474462873No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG2860.24657723212746147No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG2570.2215746456529986No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGTGCCTG2540.2189881711901231No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC2080.17932889609269922No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGCACATGGGT2060.1776045797841156No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGTCCCTGTGCTGCGGCCCC1870.16122357485257097No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGTCATAGCTGCT1840.15863710038969547No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG1840.15863710038969547No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGCGCCTG1810.15605062592682No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCCTGCCTGTCCCAGG1790.15432630961823637No Hit
TGTAAAACGACGGCCAGTTCCTGGGCTTCTCTTGAGTACGGAGGAAAGTG1700.14656688622960995No Hit
TGTAAAACGACGGCTAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC1560.13449667206952443No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGGCCCCTGAAAGACAACAG1540.1327723557609408No Hit
TGTAAAACGACGGCCAGTACACATTCTTGTAAAGCATGGGATTGCTGAAT1540.1327723557609408No Hit
TGTAAAACGACGGCTAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC1450.12501293237231437No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGTCATAGCTGTT1320.11380487636652069No Hit
TGNAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGCGAGACGGGCACC1280.11035624374935338No Hit
TGTAAAACGACGGCTAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT1270.10949408559506155No Hit
TGTAAAACGACGGCTAGTGAGCTGCTGACGCACTGTGACTCCAGCCAGAG1200.1034589785150188No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCACGGTCACTG1200.1034589785150188No Hit
TGTAAAACGACGGCTAGTGGGGAGTGAGAGGCCATAGCTGTCTGGCATGG1190.10259682036072697No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGCGTGGCGCCCGCTTTCC1170.10087250405214332No Hit
TGTAAAACGACGGCCAGTTCCATCCACTGCTGAGAAATCTCCACAACAGC1160.10001034589785149No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)