FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124593
Sequences flagged as poor quality0
Sequence length300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNTGGAAACCCATGCCATGCACTCAACACTACAA6590.5289221705874327No Hit
NNGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT5650.4534765195476471No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC4730.3796360951257294No Hit
NNGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA4550.3651890555649194No Hit
NNGGANNNNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA4470.3587681490934483No Hit
NNGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT4340.3483341760773077No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT4250.3411106562969027No Hit
NNGGANNNNGNTATGANNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT4150.3330845232075638No Hit
NNGGANNNNGNTATGNNNCGACATATCCGAGCCTCACGTTCTAGTTTCTG3720.2985721509234066No Hit
NNGGANNNNGNTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA3630.29134863114300164No Hit
NNGGANNNNGNTATGNNNACAGTGTCCCCATCAGGCTCCGGGACAGCATC3610.2897434045251338No Hit
NNGGANNNNGNTATGNNNTGGAGTTTGAAACAGCACCTTGGCACTCCTTC3600.2889407912162No Hit
NNGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC3520.28251988474472883No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAATT3380.2712832984196544No Hit
NNGGANNNNGNTATGNNNCCCTGCGGCTCCTTTCAAGTGCAGAGGAAGCC3310.26566500525711717No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT3290.2640597786392494No Hit
NNGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC3270.2624545520213816No Hit
NNGGANNNNGNTATGANNTGGAGTTTGAAACAGCACCTTGGCACTCCTTC3240.2600467120945799No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT3180.25523103224097665No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC3010.2415866059891005No Hit
NNGGANNNNGNTATGNNNAGCGTCTCATTCCATAAAGGCCACCATGAAAC2990.23998137937123273No Hit
NNGGANNNNGNTATGNNNAAGGACAAGGTGAGGCAGCACCCCACCCTGCT2930.2351656995176294No Hit
NAGGANNNNGNTATGNNNTGGAAACCCATGCCATGCACTCAACACTACAA2890.23195524628189385No Hit
NNGGANNNNGNTATGNNNCTTGAGCTCCTCGGACACGGCAGGAAACAGCT2880.23115263297295996No Hit
NNGGANNNNGNTATGANNCCCTGCGGCTCCTTTCAAGTGCAGAGGAAGCC2870.23035001966402605No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT2820.22633695311935664No Hit
NNGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT2780.22312649988362104No Hit
NNGGANNNNGNTATGANNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT2770.22232388657468719No Hit
NNGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA2680.2151003667942822No Hit
NNGGANNNNGNTATGNNNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2620.21028468694067887No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCAGGAAACAGCT2570.2062716203960094No Hit
NNGGANNNNGNTATGNNNACCTTTACCCAAATAGCTACCACCAGTGCCTT2530.20306116716027386No Hit
NNGGANNNNGNTATGANNTCCCTTAAACTTGGGATTGCTCCATTGCACTC2530.20306116716027386No Hit
NNGGANNNNGNTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2500.2006533272334722No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA2460.1974428739977366No Hit
NAGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT2440.19583764737986886No Hit
NNGGANNNNGNTATGANNAGCGTCTCATTCCATAAAGGCCACCATGAAAC2420.1942324207620011No Hit
NNGGANNNNGNTATGNNNCTTGGTGGAGCCATAGGCCGTCGTTTTACATG2390.1918245808351994No Hit
NNGGANNNNGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG2370.19021935421733163No Hit
NNGGANNNNGNTATGNNNGATGCTCCTTCCTTGGCCCCAGGCCCTGGCGT2360.18941674090839775No Hit
NNGGANNNNGNTATGNNNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG2310.1854036743637283No Hit
NNGGANNNNGNTATGNNNAGGTTGCTGGATTCAGTTTGCTGACCAACTTT2280.18299583443692663No Hit
NNGGANNNNGNTATGANNAAGGACAAGGTGAGGCAGCACCCCACCCTGCT2270.18219322112799274No Hit
NNGGANNNNGNTATGNNNACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA2270.18219322112799274No Hit
NNGGANNNNGNTATGNNNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT2250.18058799451012497No Hit
NNGGANNNNGNTATGNNNTGCTGCCGAGAGAGGACAGTCCCCAACCATAG2190.17577231465652166No Hit
NNGGANNNNGNTATGANNACCTTTACCCAAATAGCTACCACCAGTGCCTT2190.17577231465652166No Hit
NNGGANNNNGNTATGNNNATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC2170.17416708803865386No Hit
NAGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC2170.17416708803865386No Hit
NNGGANNNNGNTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA2120.17015402149398443No Hit
NNGGANNNNGNTATGNNNTGCTGGGAGAGGGGGATGAGTAGACAGGGCCA2100.16854879487611663No Hit
NNGGANNNNGNTATGNNNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG2090.16774618156718274No Hit
NNGGANNNNGNTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA2040.1637331150225133No Hit
NNGGANNNNGNTATGANNCTTGAGCTCCTCGGACACGGCAGGAAACAGCT2040.1637331150225133No Hit
NNGGANNNNGNTATGANNATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC2010.16132527509571162No Hit
NNGGANNNNGNTATGNNNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2000.16052266178677776No Hit
NAGGANNNNGNTATGNNNCCCTGCGGCTCCTTTCAAGTGCAGAGGAAGCC1980.15891743516890997No Hit
NNGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGGGCACGGGAACA1980.15891743516890997No Hit
NNGGANNNNGNTATGNNNGGGGAGACTGGGCCATACGATAGGCTTAATCC1950.1565095952421083No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1940.1557069819331744No Hit
NNGGANNNNGNTATGNNNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA1940.1557069819331744No Hit
NAGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT1910.15329914200637276No Hit
NNGGANNNNGNTATGNNNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1880.15089130207957108No Hit
NAGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA1830.14687823553490165No Hit
NNGGANNNNGNTATGNNNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT1810.14527300891703385No Hit
NNGGANNNNGNTATGANNGATGCTCCTTCCTTGGCCCCAGGCCCTGGCGT1790.14366778229916607No Hit
NAGGANNNNGNTATGNNNAGCGTCTCATTCCATAAAGGCCACCATGAAAC1780.1428651689902322No Hit
NNGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCAGGAAACAGCT1760.14125994237236442No Hit
NAGGANNNNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA1740.13965471575449664No Hit
NNGGANNNNGNTATGANNTGCTGCCGAGAGAGGACAGTCCCCAACCATAG1720.13804948913662887No Hit
NAGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1720.13804948913662887No Hit
NNGGANNNNGNTATGNNNACCGTTAAGCCACACCAAATGGTGAGCCCTGC1710.13724687582769496No Hit
NNGGANNNNGNTATGNNNCTCAGCTCTGCAGCCTCCGCACAGACCCCACA1710.13724687582769496No Hit
NNGGANNNNGNTATGANNAGGTTGCTGGATTCAGTTTGCTGACCAACTTT1700.1364442625187611No Hit
NNGGANNNNGNTATGNNNTGACAGCACAAGGAACACTGACCCGAGGCAGG1700.1364442625187611No Hit
NNGGANNNNGNTATGANNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG1680.1348390359008933No Hit
NNGGANNNNGNTATGANNACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA1670.13403642259195941No Hit
NNGGANNNNGNTATGNNNCATGGTTGGGGAAAAACTCCTCATCTTCATGT1650.13243119597409164No Hit
NAGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAATT1630.13082596935622387No Hit
NNGGANNNNGNTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1620.13002335604728996No Hit
NNGGANNNNGNTATGANNCTTGGTGGAGCCATAGGCCGTCGTTTTACATG1600.12841812942942218No Hit
NAGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT1600.12841812942942218No Hit
NNGGANNNNGNTATGNNNCATGGTTGGGGAAAAACTCCTCATTTTCATGT1590.12761551612048833No Hit
NNGGANNNNGNTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1590.12761551612048833No Hit
NNGGANNNNGNTATGNNNAGGGCAAGTGTTCGCGTTGTGACAGTTACGGT1580.1268129028115544No Hit
NNGGANNNNGNTATGANNTGCTGGGAGAGGGGGATGAGTAGACAGGGCCA1580.1268129028115544No Hit
NAGGANNNNGNTATGNNNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1570.12601028950262055No Hit
NNGGANNNNGNTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1550.12440506288475274No Hit
NAGGANNNNGNTATGNNNTGGAGTTTGAAACAGCACCTTGGCACTCCTTC1540.12360244957581887No Hit
NAGGANNNNGNTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA1540.12360244957581887No Hit
NAGGANNNNGNTATGANNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT1530.12279983626688497No Hit
NAGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC1490.11958938303114942No Hit
NNGGANNNNGNTATGNNNAGCAAAGGTCTTGGCAGGGGTGGGGACTGGAA1490.11958938303114942No Hit
NNGGANNNNGNTATGNNNAGCTGGACGGGGTCCTTCTTAGGGTCTGGGGC1490.11958938303114942No Hit
NNGGANNNNGNTATGANNTGACAGCACAAGGAACACTGACCCGAGGCAGG1470.11798415641328165No Hit
NNGGANNNNGNTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT1460.11718154310434775No Hit
NAGGANNNNGNTATGNNNATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC1460.11718154310434775No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACCCTTTGTCCA1440.11557631648647998No Hit
NNGGANNNNGNTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT1430.1147737031775461No Hit
NAGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC1410.11316847655967832No Hit
NAGGANNNNGNTATGNNNAAGGACAAGGTGAGGCAGCACCCCACCCTGCT1410.11316847655967832No Hit
NNGGANNNNGNTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA1390.11156324994181052No Hit
NNGGANNNNGNTATGANNGGTAGCAACGTCCCACCCAGGGCACGGGAACA1380.11076063663287665No Hit
NNGGANNNNGNTATGNNNTCCCACCACTGACTCCAGGGGAAGGAGGAGGA1370.10995802332394275No Hit
NNGGANNNNGNTATGANNAGGGCAAGTGTTCGCGTTGTGACAGTTACGGT1350.10835279670607498No Hit
NAGGANNNNGNTATGNNNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1340.1075501833971411No Hit
NAGGANNNNGNTATGNNNACCTTTACCCAAATAGCTACCACCAGTGCCTT1340.1075501833971411No Hit
NNGGANNNNGNTATGNNNATCGCACCACTGCACTCCAGCGCTCCATACAC1320.1059449567792733No Hit
NAGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA1310.10514234347033943No Hit
NAGGANNNNGNTATGANNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT1310.10514234347033943No Hit
NNGGANNNNGNTATGANNGGGGAGACTGGGCCATACGATAGGCTTAATCC1300.10433973016140553No Hit
NAGGANNNNGNTATGNNNAGGTTGCTGGATTCAGTTTGCTGACCAACTTT1300.10433973016140553No Hit
NNGGANNNNGNTATGANNAGCAAAGGTCTTGGCAGGGGTGGGGACTGGAA1260.10112927692566998No Hit
NNGGANNNNGNTATGANNCATGGTTGGGGAAAAACTCCTCATCTTCATGT1250.1003266636167361No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)