FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88876
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC21332.3999729960844323No Hit
TGTAAAACGACGGCCAGTAGCACGAAAAGTAGGTGGCCTTACTGGTAGCA18312.0601737251901526No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC15481.7417525541203474No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT13541.5234709032809757No Hit
TGTAAAACGACGGCCAGTTGAGAGGGACTGGAGAAAGGGAACTTACAGAG13301.4964669877132184No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG13081.4717133984427744No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC12991.4615869301048652No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG12521.4087042621180073No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA12501.406453935820694No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC12171.3693235519150275No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC11151.254556910752059No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC10901.2264278320356452No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC10791.214051037400423No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA10421.1724200009001307No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA10131.1397902695890905No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA9921.1161618434673028No Hit
TGTAAAACGACGGCCAGTTGAGAAGCTGAGGCTTAAAGAGGGATCCCTTG9671.088032764750889No Hit
TGTAAAACGACGGCCAGTGGGGTGGGGAGTGAGATGGTCCTAGGAAGTAC9181.0328997704667175No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCGTGACAAGGCT8911.0025203654529908No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC8590.966515144695981No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC8590.966515144695981No Hit
TGTAAAACGACGGCCAGTTGGATGGTGAGGGAAGGGCTAAGACAGGATTC8550.9620144921013547No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGGCTTCTGGGCTT8520.9586390026553849No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGC8500.9563886763580718No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG8490.9552635132094154No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC8450.9507628606147891No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG8420.9473873711688195No Hit
TGTAAAACGACGGCCAGTGAGACAGGCTTCTGGGCTGTCCACAACTCTCT8250.9282595976416581No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCATGACAAGGCT8200.9226337818983753No Hit
TGTAAAACGACGGCCAGTCCCAGCACAACTTCCCCCATGGCGTGGTGTCC7620.8573743192762951No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC7580.8528736666816689No Hit
TGTAAAACGACGGCCAGTGGTGTTACCGTGGTCACCCGCAGGGACACGGA7290.8202439353706287No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG7270.8179936090733155No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC7250.8157432827760025No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG7170.8067419775867501No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT7080.7966155092488411No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC6920.7786128988703362No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG6520.733606372924074No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCCAAGAGGTGGGG6430.723479904586165No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG6150.6919753364237814No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG6100.6863495206804987No Hit
TGTAAAACGACGGCCAGTAAGGGTGGGTCATGGGGTGAGGGTGGGTCGCG6030.6784733786399028No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC5950.6694720734506504No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA5900.6638462577073676No Hit
TGTAAAACGACGGCCAGTCTGTCTGGCATGGGCCTCTCCACCGTGCCTGA5540.6233403843557316No Hit
TGTAAAACGACGGCCAGTTCATAGCTGTGAAGAGGGATACCAAGGAAAGT5360.6030874476799136No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA5350.601962284531257No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC5220.5873351635987218No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCCGTGGG5220.5873351635987218No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATGGCCGAGCAGGG5190.5839596741527522No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT5120.5760835321121562No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG5120.5760835321121562No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC4790.53895314820649No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG4700.5288266798685809No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCAGTGGG4660.5243260272739547No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG4640.5220757009766417No Hit
TGTAAAACGACGGCCAGTACACATTCTTGTAAAGCATGGGATTGCTGAAT4620.5198253746793285No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTTGGACCCAGAG4550.5119492326387326No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC4440.4995724380035105No Hit
TGTAAAACGACGGCCAGTCCACCTGCTTCGCACCATCTCGGCCCAGCCCA4390.4939466222602278No Hit
TGTAAAACGACGGCCAGTTTGCTGCTGGGCTTCCTCTGCCCTGAGGGCTT4230.47594401188172286No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC4020.4523155857599352No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCAAGAGGTGGGGT3940.4433142805706828No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG3840.4320626490841172No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT3550.39943291777307705No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA3520.39605742832710744No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG3500.39380710202979435No Hit
TGTAAAACGACGGCCAGTGGCGCTGACGAACAGAGGAAGCGAGTACAACG3480.3915567757324812No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCACATGCCTCCC3480.3915567757324812No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT3430.3859309599891984No Hit
TGTAAAACGACGGCCAGTCTTGCACTACATGGCCATCAGGGTCTCGTCCC3420.38480579684054184No Hit
TGTAAAACGACGGCCAGTCCCTGTCCTGAGCACCGTTGCTAACCCCCAGT3310.37242900220531977No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT3150.3544263918268149No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGGGGCCCGGATGT3020.3397992708942797No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCATCAGGTTCCC3010.3386741077456231No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT2940.33079796570502723No Hit
TGTAAAACGACGGCCAGTTCCATTACTCTACACAAGCAGGGTACACTTAG2780.3127953553265223No Hit
TGTAAAACGACGGCCAGTGCCATCGGCTCCCAAGTTTTATGTCCTAGAGC2700.3037940501372699No Hit
TGTAAAACGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA2680.3015437238399568No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTCCCTTCTCTTTG2550.2869166029074216No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGGGGACCTGCTGGAG2530.28466627661010846No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGATATCTCCTGCAC2460.27679013456951257No Hit
TGTAAAACGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT2300.2587875241910077No Hit
TGTAAAACGACGGCCAGTTGATGACCTCTTGGCTAACATACACAACATGA2070.23290877177190691No Hit
TGTAAAACGACGGCCAGTCTTGCACTACATGGCCATCATGAACCAGCGAA2060.23178360862325034No Hit
TGTAAAACGACGGCCAGTAGATCATCCAGCGCACACGGCGTCTTGGCGGG2000.22503262973131102No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGGCTGTCATCTCT2000.22503262973131102No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCTTGTGACAATGA1990.22390746658265448No Hit
TGTAAAACGACGGCCAGTCTGGGCCTTGGCTTCTTCCTCAATCATCTAGG1970.2216571402853414No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTGGTGGTCC1950.21940681398802828No Hit
TGTAAAACGACGGCCAGTCCCTGTCCTGAGCACCGTTGGCTCCAGGATCG1890.21265583509608893No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCGTCTACACGAGA1850.2081551825014627No Hit
TGTAAAACGACGGCCAGTGCCAATCCTGAGGCCATCAAAGTGACAGGTAG1850.2081551825014627No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC1790.20140420360952338No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGCATTTCCTTGGC1790.20140420360952338No Hit
TGTAAAACGACGGCCAGTCCCCCAGAAAATTGTGCCAAAGAGTTTAGAAA1750.19690355101489718No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCAGCAGGATGGAG1660.18677708267698817No Hit
TGTAAAACGACGGCCAGTTTTCCCCTTCTCCCTCCAGGGGTGCTTTCTCT1650.1856519195283316No Hit
TGTAAAACGACGGCCAGTACCTCATGTCACGTGCGTCCATGTGAAGAGAG1630.1834015932310185No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTTCCTATCG1610.18115126693370537No Hit
TGTAAAACGACGGCCAGTTGGCCCACTGTGCTAGCCTAGGATTTTTTTTT1510.16989963544713985No Hit
TGTAAAACGACGGCCAGTGACTGTAGGTTGGGCTCCCCATGTGCTGTGCC1430.16089833025788738No Hit
TGTAAAACGACGGCCAGTGACCTCATGGGGCGCTTCCGCGTGCTGGGCTG1420.15977316710923084No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCACTGCCTAGCAGG1370.15414735136594807No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGCCCCAATCCTGTCTC1270.14289571987938252No Hit
TGTAAAACGACGGCCAGTACAAAAGTTCTCCAAGTCCCTAGATTTAGCTA1260.14177055673072594No Hit
TGTAAAACGACGGCCAGTTGTGGAAGTCGGGGACATAAGATGATTTTCAC1220.13726990413609974No Hit
TGTAAAACGACGGCCAGTGGGGTGGGGAGTGAGATGGTGCCTGAAAGAAG1220.13726990413609974No Hit
TGTAAAACGACGGCCAGTGACTGTAGGTTGGGCTCCCCGAGGATCCTCCC1150.12939376209550385No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTA1150.12939376209550385No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGCGCAGCCCTCCTAC1130.12714343579819073No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCCCAACCCCTCAG1130.12714343579819073No Hit
TGTAAAACGACGGCCAGTTTACAGGTGTGAGCCACCGCGCCCGGCTGGGA1070.1203924569062514No Hit
TGTAAAACGACGGCCAGTTCCCACCACCATACCCCAACTTAGTATTTGAA1070.1203924569062514No Hit
TGTAAAACGACGGCCAGTACTAAACTGTAGTGAACAGAGACATGCACGAT1030.11589180431162517No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTCCTTAAGTATGC990.11139115171699895No Hit
TGTAAAACGACGGCCAGTAGCCCTGCTGTGAAATGATGGAAATGCATTGG990.11139115171699895No Hit
TGTAAAACGACGGCCAGTGGTCCCACTCTGGCTTTCCCACCTGATAATAA940.10576533597371619No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGCGCCTG900.10126468337908996No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)