FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152485
Sequences flagged as poor quality0
Sequence length300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC60543.970226579663574No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC53713.5223136701970685No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC48893.206217004951307No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCCGTGGAGGTGG36222.375315604813588No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTCACCATTTGGTG33512.197593205889104No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC33312.1844771616880347No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT32092.1044692920615145No Hit
TGTAAAACGACGGCCAGTGGGGTGGGGAGTGAGATGGTGCCTGAAAGAAG29371.9260910909269762No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC27541.8060792864871955No Hit
TGTAAAACGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT26891.7634521428337213No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAGAGCAAAGTCC25711.6860674820474146No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG24721.6211430632521233No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC24301.5935993704298785No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG23691.5535954356166182No Hit
TGTAAAACGACGGCCAGTGACTGTAGGTTGGGCTCCCCCACAGGATCCTC23221.522772731744106No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC20881.3693150145915993No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG20541.347017739449782No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTTCAGCCCAGGAG17871.1719185493655113No Hit
TGTAAAACGACGGCCAGTTGGAGTGCCTGGGTTAGAACCCTGCTGGTACC17811.1679837361051908No Hit
TGTAAAANGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC17161.1253565924517166No Hit
TGTAAAANGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC16001.0492835360855166No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCGTGGGTC15691.0289536675738598No Hit
TGTAAAANGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC15240.9994425681214546No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCTTCCACTGTCC15100.9902613371807064No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC14310.938452962586484No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCGACCACACCACA14200.931239138275896No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTATTTCCCTACTT12550.8230317736170771No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC11810.7745024100731219No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACAGGAAGCAGGTT11280.7397448929402892No Hit
TGTAAAANGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC11090.7272846509492737No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG10550.6918713316063876No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG10340.6780994851952651No Hit
TGTAAAANGACGGCCAGTGTGGTAACTGCTGCAGGGCTCACCATTTGGTG9860.6466209791126996No Hit
TGTAAAANGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT9790.6420303636423255No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCCAGACCCAAACT9680.6348165393317375No Hit
TGTAAAANGACGGCCAGTGCTAGACATCGCCTGGTGCCCCGTGGAGGTGG9520.6243237039708823No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA8510.5580876807554842No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGAAAGCCTCAGGCA8450.5541528674951635No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTGACTCAAATGTT8350.547594845394629No Hit
TGTAAAANGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT8310.5449716365544152No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCAGCTCACT8260.5416926255041479No Hit
TGTAAAANGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG8240.5403810210840411No Hit
TGTAAAANGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG8240.5403810210840411No Hit
TGTAAAANGACGGCCAGTGGGGTGGGGAGTGAGATGGTGCCTGAAAGAAG8020.5259533724628651No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC7950.521362756992491No Hit
TGTAAAANGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAGAGCAAAGTCC7700.5049677017411549No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCGGCTCACT7690.5043118995311014No Hit
TGTAAAANGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC7270.4767682067088566No Hit
TGTAAAANGACGGCCAGTGACTGTAGGTTGGGCTCCCCCACAGGATCCTC7010.459717349247467No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAGAGCAAAGTC6750.4426664917860773No Hit
TGTAAAANGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC6740.44201068957602385No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGTGCTTGACTG6200.4065973702331377No Hit
TGTAAAANGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG6120.40135095255271014No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG6110.4006951503426567No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTAGCCTCTCCATT5560.364626028789717No Hit
TGTAAAANGACGGCCAGTTGGAGTGCCTGGGTTAGAACCCTGCTGGTACC5490.3600354133193429No Hit
TGTAAAANGACGGCCAGTCCGCCCTTTCCAAGCTCACTTCAGCCCAGGAG5380.35282158900875493No Hit
TGTAAAANGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCGTGGGTC5240.3436403580680067No Hit
TGTAAAANGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC5110.33511492933731185No Hit
TGTAAAACGACGGCCAGTGGGGACAAGGGTGGCTTTGTAAATAGCAGCAA5060.3318359182870446No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGTGGTCACCTG5000.3279011050267239No Hit
TGTAAAACGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG4950.3246220939764567No Hit
TGTAAAANGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC4730.31019444535528085No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGACGGCACTGGTG4350.28527396137324984No Hit
TGTAAAANGACGGCCAGTGCTAGACATCGCCTGGTGCCGACCACACCACA4190.2747811260123946No Hit
TGTAAAANGACGGCCAGTCCGCCCTTTCCAAGCTCACTATTTCCCTACTT4060.26625569728169984No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGTGCCTG4050.2655998950716464No Hit
TGTAAAANGACGGCCAGTCCTGCGCTATGGTTGGGGACCTTCCACTGTCC3900.2557628619208447No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGTGGGGTCACCCC3800.24920483982031016No Hit
TGTAAAANGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG3720.2439584221398826No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT3540.23215398235892057No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGAAGCCTCAACCC3530.23149818014886708No Hit
TGTAAAANGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG3340.21903793815785158No Hit
TGTAAAACGACGGCCAGTGCCAGACATCGCCTGGTGCCCACACCACAGCC3310.21707053152769124No Hit
TGTAAAANGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA3280.2151031248975309No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCCGTGGAGGTCG3180.20854510279699642No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACAGCTGCCCATGG3120.20461028953667576No Hit
TGTAAAANGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC3060.20067547627635507No Hit
TGTAAAANGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCGGCTCACT2910.19083844312555334No Hit
TGTAAAANGACGGCCAGTCCTGCGCTATGGTTGGGGACAGGAAGCAGGTT2760.18100140997475161No Hit
TGTAAAANGACGGCCAGTACAGCTTGGCACAGGCTCTCCAGACCCAAACT2720.1783782011345378No Hit
TGTAAAANGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC2670.17509919008427058No Hit
TGTAAAANGACGGCCAGTGCAGGTGCAGCTGCCACAGAAAGCCTCAGGCA2600.17050857461389646No Hit
TGTAAAACGACGGCCAGTCCTTGCCTGTGAGCGTGTGGCGCCCGCTTTCC2570.1685411679837361No Hit
TGTAAAANGACGGCCAGTCCGCCCTTTCCAAGCTCACTGACTCAAATGTT2320.1521461127323999No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGACACC2240.1468996950519723No Hit
TGTAAAACGACGGCCAGTGCCAGACATCGCCTGGTGCCCCGTGGAGGTGG2210.14493228842181197No Hit
TGTAAAANGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCAGCTCACT2210.14493228842181197No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCGCCAGGTCAC2150.1409974751614913No Hit
TGTAAAANGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGTGCTTGACTG2000.13116044201068958No Hit
TGTAAAANGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAGAGCAAAGTC2000.13116044201068958No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG1870.12263501327999474No Hit
TGTAAAACGACGGCCAGTGCCAATCCTGAGGCCATCAAAGTGACAGGTAG1870.12263501327999474No Hit
TGTAAAANGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG1720.11279798012919302No Hit
TGTAAAANGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGTGCCTG1570.10296094697839131No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGCGCAGCCCTCCTAC1570.10296094697839131No Hit
TGTAAAANGACGGCCAGTGGGGACAAGGGTGGCTTTGTAAATAGCAGCAA1540.10099354034823096No Hit
TGTAAAACGACGGCCAGTTCCCAGGAGAGCCCAAAGGGAGAGCAAAGTCC1540.10099354034823096No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)