FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124825
Sequences flagged as poor quality0
Sequence length300
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG31132.493891448027238No Hit
TGTAAAACGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT25362.031644302022832No Hit
TGTAAAACGACGGCCAGTTCTTTGGCTCTACCTAAAAGTTGGTCAGCAAA23951.9186861606248748No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG23581.8890446625275386No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA22431.7969156819547367No Hit
TGTAAAACGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG21241.7015822151011415No Hit
TGTAAAACGACGGCCAGTCCAGAGATCAATAATAGCTCAGGGGAGATGGA21001.682355297416383No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA19581.5685960344482275No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGGCAGCACCTCT19251.5421590226316844No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGC18441.4772681754456238No Hit
TGTAAAACGACGGCCAGTTCACAGTGCTGTAGGGGCCTACACGTTCCAGG18181.4564390146204687No Hit
TGTAAAACGACGGCCAGTGCCACACGAGGCTTCATTCGTTATGGCTGGCG17521.4035649909873822No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG16541.325055077107951No Hit
TGTAAAACGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT16121.2914079711596236No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT15861.2705788103344682No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCCAAGAGGTGGGG15451.2377328259563387No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT14941.1968756258762268No Hit
TGTAAAACGACGGCCAGTACATGCGTGGCCCTGTCTACTCATCCCCCTCT14901.1936711395954336No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG14811.186461045463649No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT14761.1824554376126577No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG14071.1271780492689767No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC13981.1199679551371922No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA12671.0150210294412176No Hit
TGTAAAACGACGGCCAGTGCCACTTCTGAAGGAGGTTCCCCCAGCAGTGC11860.9501301822551572No Hit
TGTAAAACGACGGCCAGTCCCTGTCCTGAGCACCGTTGCTAACCCCCAGT11210.8980572801922693No Hit
TGTAAAACGACGGCCAGTGGCTCTCATCCTCTTCTGCAACTACTATGTTT11130.891648307630683No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG10480.8395754055677949No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCTGGAGGGCGGCCA10330.8275585820148208No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG10290.8243540957340277No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCACATGCCTCCC10040.8043260564790707No Hit
TGTAAAACGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT9700.7770879230923292No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCAAGAGGTGGGGT9360.7498497897055878No Hit
TGTAAAACGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT9020.7226116563188464No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT8880.7113959543360705No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT8790.704185860204286No Hit
TGTAAAACGACGGCCAGTAGCAGGCAGGTCCTTCCAGAACCCACTTAAGA8250.660925295413579No Hit
TGTAAAACGACGGCCAGTCCTGTGTTTCCACTTCTGCTTCCAAAGATTTT8090.6481073502904066No Hit
TGTAAAACGACGGCCAGTGGTGTTACCGTGGTCACCCGCAGGGACACGGA8060.6457039855798118No Hit
TGTAAAACGACGGCCAGTTGGAGTGCCTGGGTTAGAACCCTGCTGGTACC7970.6384938914480273No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGGTGCTCTGGT7690.6160624874824754No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG7170.574404165832165No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGTGAACGAAG7170.574404165832165No Hit
TGTAAAACGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC7160.5736030442619668No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG6540.5239335069096736No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTAGAGCCTGGCT6490.5199278990586822No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCACCTTGGCCTCC6270.50230322451432No Hit
TGTAAAACGACGGCCAGTAGGTGGCAAAGCTCAATCAGGACAGTTCTGAA6230.4990987382335269No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCGTGGGTC6160.493490887242139No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC6070.48628079311035455No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC5850.4686561185659924No Hit
TGTAAAACGACGGCCAGTCCCTCCAGCTGCTCTCTGCCCAGCGGCAGCAG5730.459042659723613No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA5710.45744041658321644No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGTCCCTGTTGCGGCCCCTG5580.4470258361706389No Hit
TGTAAAACGACGGCCAGTCTGGGTTGGGGTGTCTGCTGCTCCAGGATGGC5540.4438213498898458No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG5330.42699779691568196No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGGGCTTGACACGG5280.42299218906469055No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTCCCGACGCCAGG5200.4165832165031043No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG5100.4085720008011216No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCTCCTTCCCAT5050.4045663929501302No Hit
TGTAAAACGACGGCCAGTCTCTCACCCACCTCCACCCATCAAAGGTGCCT4920.39415181253755255No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCTCCTGCTCAGCCTT4650.3725215301421991No Hit
TGTAAAACGACGGCCAGTCCCTGTCCTGAGCACCGTTGGCTCCAGGATCG4600.36851592229120766No Hit
TGTAAAACGACGGCCAGTAGGCTTTGGGAGGCCATGACTAGTTAGAGACA4590.36771480072100937No Hit
TGTAAAACGACGGCCAGTAGCAGGCAGGTCCTTCCAGACAGCCAATGAGG4230.3388744241938714No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG4150.3324654516322852No Hit
TGTAAAACGACGGCCAGTCGCAGAAGCAAGGGGCTGAAAAGATCTGAAGG4080.3268576006408973No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGGTTGGAGGTG4060.3252553575005007No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGGGGAAGGAGAGT4030.3228519927899059No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGTCCCTGTTTTACAATGCA4020.3220508712197076No Hit
TGTAAAACGACGGCCAGTTGTCTGGTGGTGGAAGGAGGGGAGATGGGGAG4010.3212497496495093No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGGCATTGGTCTGG3970.3180452633687162No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCACCTCAGCCTCC3750.30042058882435413No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCATCAGGTTCCC3740.29961946725415584No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTGACTCAAATGTT3690.2956138594031644No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTTCAGCCCAGGAG3680.29481273783296613No Hit
TGTAAAACGACGGCCAGTGGATGCCAAGGCAGTACCTGAGCCGAAGCTGT3540.2835970358501903No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGCGTCATGGCT3470.2779891848588023No Hit
TGTAAAACGACGGCCAGTCTGGGTTGGGGTGTCTGCTGGATGAAACCTCA3430.2747846985780092No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC3210.2571600240336471No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGTCATAGCTGTT3180.25475665932305225No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTCCCTTCTCTTTG3160.2531544161826557No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCACCGAGGCAG3120.2499499299018626No Hit
TGTAAAACGACGGCCAGTCCCCCAGAAAATTGTGCCAAAGAGTTTAGAAA3000.24033647105948328No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCGCCAAGGCCTCC3000.24033647105948328No Hit
TGTAAAACGACGGCCAGTGGCTCTCATCCTCTTCTGCAACTCAACAATGA2810.225115161225716No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCGCCTCCTGCTCAGCC2770.2219106749449229No Hit
TGTAAAACGACGGCCAGTTGTCTGGTGGTGGAAGGAGGAGGTTGCAGTGA2770.2219106749449229No Hit
TGTAAAACGACGGCCAGTTGTCTGGTGGTGGAAGGAGGTGCACGCCACCA2700.21630282395353495No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTATTTCCCTACTT2690.21550170238333666No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGTCCCTGTGCGGCCCCTGA2680.2147005808131384No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGGGGAGGCGCCAG2660.21309833767274183No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGACCCTTTGCCCC2650.21229721610254354No Hit
TGTAAAACGACGGCCAGTCTGGGCCTTGGCTTCTTCCTCAATCATCTAGG2530.20268375726016424No Hit
TGTAAAACGACGGCCAGTCTCTCACCCACCTCCACCCATCAACAGGCCCC2440.19547366312837972No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGTGTCGTGGGGCA2270.181854596435009No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCTCGCCTCCTCTG2270.181854596435009No Hit
TGTAAAACGACGGCCAGTGGCTCTCATCCTCTTCTGCAACTGTAAAACGA2240.17945123172441418No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTTGGACCCAGAG2160.17304225916282798No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTGGTGGGGTTAGG2080.16663328660124174No Hit
TGTAAAACGACGGCCAGTTCCCACCACCATACCCCAACTCAGGCGGGAGG2020.16182655718005207No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGCATTTCCTTGGC1970.15782094932906068No Hit
TGTAAAACGACGGCCAGTAGCAGGCAGGTCCTTCCAGAGCAGTCCCTGTT1900.15221309833767274No Hit
TGTAAAACGACGGCCAGTAGCAGGCAGGTCCTTCCAGACCCCCAGGGATA1860.14900861205687962No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGTCCCTGTCGGCGGCCCCT1850.14820749048668136No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGAGGCTCCTTCC1850.14820749048668136No Hit
TGTAAAACGACGGCCAGTGGATGCCAAGGCAGTACCTGAAGCACCGGGGA1830.1466052473462848No Hit
TGTAAAACGACGGCCAGTAGAACAATGCGGCACTGAAGGAAGAGATGATT1770.14179851792509515No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC1710.13699178850390548No Hit
TGTAAAACGACGGCCAGTCAGTGTCCGCAGAAGCAAGGGGCTGAAAAGAT1700.13619066693370718No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCACTGCCTAGCAGG1700.13619066693370718No Hit
TGTAAAACGACGGCCAGTAGCCCCTCGTCACCTACTCCTTTGCTTTGGGG1650.1321850590827158No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCTCCCTGTGCCTC1600.12817945123172442No Hit
TGTAAAACGACGGCCAGTGGATGCCAAGGCAGTACCTGAAGCACCAGGGA1550.12417384338073302No Hit
TGTAAAACGACGGCCAGTACCTCATGTCACGTGCGTCCATGTGAAGAGAG1520.1217704786701382No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCCCCTCAGCCTGC1490.11936711395954336No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGGCACCTCTGAC1470.1177648708191468No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGCCGGCAGCAATC1450.11616262767875024No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCAGGGAGACCCTCC1450.11616262767875024No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCAAGCTGGCTGT1440.11536150610855196No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCAGGATGGTGTGGC1420.11375926296815543No Hit
TGTAAAACGACGGCCAGTACATGCGTGGCCCTGTCTACGGCCCCGCCCTC1390.11135589825756059No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA1380.11055477668736231No Hit
TGTAAAACGACGGCCAGTCCTCACTCCCCACCCAGGATGTAAAACGACGG1370.10975365511716403No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCTGAGAAGGACCT1360.10895253354696575No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGCACTTGGGGGTT1360.10895253354696575No Hit
TGTAAAACGACGGCCAGTGGGGACAAGGGTGGCTTTGTAAATAGCAGCAA1310.10494692569597436No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCATTTCTCCTTC1310.10494692569597436No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCACAGCACTGCC1290.10334468255557781No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCGCCTTGGCCTCT1290.10334468255557781No Hit
TGTAAAACGACGGCCAGTAGCCCCTCGTCACCTACTCCCCGAAGTTACCA1260.10094131784498298No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)