FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124825
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT28922.3168435810134187No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT25122.012417384338073No Hit
CAGGAAACAGCTATGACCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC23501.8826356899659522No Hit
CAGGAAACAGCTATGACCAGGTTGCTGGATTCAGTTTGCTGACCAACTTT22621.812136991788504No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC21181.6967754856799517No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA21031.6847586621269777No Hit
CAGGAAACAGCTATGACCGGGGAGACTGGGCCATACGATAGGCTTAATCC19111.5309433206489085No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCTGCCCAACACCC18941.5173242539555378No Hit
CAGGAAACAGCTATGACCCCCGTGACAGCTCCTGCAATTACAAAATGGCT16691.3370719006609253No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT16611.330662928099339No Hit
CAGGAAACAGCTATGACCGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC16381.3122371319847785No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGGACACTGCCCAC15181.2161025435609853No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGGCACGGGAACA14381.152012817945123No Hit
CAGGAAACAGCTATGACCTGCTGGGAGAGGGGGATGAGTAGACAGGGCCA14261.142399359102744No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC13971.1191668335669938No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT13161.0542759863809334No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT13071.047065892249149No Hit
CAGGAAACAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT11580.9276987782896053No Hit
CAGGAAACAGCTATGACCTGACTGAAAGGCTTTTCCACGTGCACTGCTGG11160.8940516723412778No Hit
CAGGAAACAGCTATGACCTCCCTTAAACTTGGGATTGCTCCATTGCACTC10810.8660124173843381No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCATA10740.8604045663929502No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA10660.8539955938313639No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA10040.8043260564790707No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG9770.7826957740837172No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC9710.7778890446625275No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTCCCCAACCATAG9590.7682755858201482No Hit
CAGGAAACAGCTATGACCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG9490.7602643701181655No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGACACTGCCCACC9280.7434408171440016No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACACAGCTGGGAGGC9010.7218105347486481No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA8520.6825555778089325No Hit
CAGGAAACAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG8470.6785499699579411No Hit
CAGGAAACAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG8440.6761466052473463No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACCAGGAAACAGCT7750.6208692169036651No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA7740.6200680953334669No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC7510.6016422992189064No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG7370.5904265972361306No Hit
CAGGAAACAGCTATGACCAGGACTTGCCCACTCTCCCAGTTACTTGGAAT7250.5808131383937513No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCCTCCCCACCTCC7220.5784097736831564No Hit
CAGGAAACAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG7080.5671940717003806No Hit
CAGGAAACAGCTATGACCTGGGTTGCCTGGGACTGAGACTTGTGGACCAA7020.5623873422791908No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT6880.551171640296415No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGAGCCT6690.5359503304626476No Hit
CAGGAAACAGCTATGACCTGGCATCCAGGGCCTATTTGGTTCCTATTGAG6640.5319447226116564No Hit
CAGGAAACAGCTATGACCCCGTAGCCACGCCCCTATTGTCTGACCACGCT6540.5239335069096736No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC6510.5215301421990787No Hit
CAGGAAACAGCTATGACCGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC6320.5063088323653114No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT5940.4758662126977769No Hit
CAGGAAACAGCTATGACCACCCCTTGCATTGGTAGGGCTTCTCTCCTGTG5750.4606449028640096No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCAACAGGGACGCA5540.4438213498898458No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAGGGGACATGGCC5330.42699779691568196No Hit
CAGGAAACAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC5220.41818545964350085No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCCCGTGTCAAGC5130.41097536551171643No Hit
CAGGAAACAGCTATGACCGATGCTCCTTCCTTGGCCCCAGGCCCTGGCGT4770.3821349889845784No Hit
CAGGAAACAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC4550.3645103144402163No Hit
CAGGAAACAGCTATGACCTCCAGCTGCTCCATCTTGTCTACCTCCCGGCC4530.3629080712998198No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT4530.3629080712998198No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCAGGAAACAGCT4520.3621069497296215No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACGGGGATGGCAGTG4520.3621069497296215No Hit
CAGGAAACAGCTATGACCCAGGGTGACTCCGATCCTGGAGCCAACGGTGC4480.35890246344882837No Hit
CAGGAAACAGCTATGACCCTGGGTGCCGGGTAGAGTGTATACAGGGCTCA4190.3356699379130783No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG4180.33486881634288No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACCCCGTTAGCTGC4150.3324654516322852No Hit
CAGGAAACAGCTATGACCAGAGCTACTTCTGTGCCCACCATATTCCTCTA3940.31564189865812137No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCAGACCAATGCC3860.30923292609653513No Hit
CAGGAAACAGCTATGACCACCTGGCAGCCCCAAATCTGCACCTCCCGCGG3780.30282395353494895No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCCATGCCTGCCGT3740.29961946725415584No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTCACACCTGTAA3710.297216102543561No Hit
CAGGAAACAGCTATGACCTTGGACAGCACCTTGAATGCTATCAGGGAAGC3610.2892048868415782No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGAGGGCATGCCTT3590.28760264370118166No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG3570.28600040056078513No Hit
CAGGAAACAGCTATGACCGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT3410.27318245543761266No Hit
CAGGAAACAGCTATGACCAGACAGGGTCTTGCTATGTTGCCCAGGTTGAT3390.2715802122972161No Hit
CAGGAAACAGCTATGACCTGGGTTGCCTGGGACTGAGAGGGTCCTGGGAC3340.2675746044462247No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAATAAATAGGCAGC3330.2667734828760265No Hit
CAGGAAACAGCTATGACCTTGGACAGCACCTTGAATGCTTTCAGGGAAGC3290.26356899659523336No Hit
CAGGAAACAGCTATGACCACCTGGCAGCCCCAAATCTGCACCTCCCGCAG3270.2619667534548368No Hit
CAGGAAACAGCTATGACCTCCCTCATTGTTGAGAGGCAGCCAGGAAACAG3250.26036451031444025No Hit
CAGGAAACAGCTATGACCAGACAGGGTCTTGCTATGTTGCCAAAGGAGAT3210.2571600240336471No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCATCGCTACACTC3180.25475665932305225No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTCACACCTATAA3050.24434207891047469No Hit
CAGGAAACAGCTATGACCAGACAGGGTCTTGCTATGTTGCCCAGGCTGGT2930.23472862006809533No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAGTAGTCT2910.23312637692769878No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA2890.23152413378730222No Hit
CAGGAAACAGCTATGACCGGAGAAGCACAGCTTCGGCTCTGGCCCTGGGG2880.23072301221710392No Hit
CAGGAAACAGCTATGACCTCCAGTGGTGGGGCTCAACAAAGGCTCCGTGA2850.2283196475065091No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTCACGTGAGCCT2770.2219106749449229No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCGCAGTGGGGCCA2680.2147005808131384No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC2670.2138994592429401No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACACACACTTCTAA2610.20909272982175042No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGCGATCTCGTCTC2590.20749048668135392No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCTATTTTCCAAAG2550.2042860004005608No Hit
CAGGAAACAGCTATGACCGCAGCAAGAGACCAATACCGCATTGTAGTCAA2540.2034848788303625No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACTGGCGCCTCCCC2510.20108151411976768No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCACAGGGACGCAG2470.19787702783897457No Hit
CAGGAAACAGCTATGACCGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG2460.19707590626877627No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCAGACACACGGC2460.19707590626877627No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCCAAACCAG2440.19547366312837972No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGACACCCAAGACA2260.18105347486481074No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACAGAGGAGGCGAGC2190.17544562387342277No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTGTAATCCCAGC2180.1746445023032245No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACGGCCCCTCTGCC2160.17304225916282798No Hit
CAGGAAACAGCTATGACCTCCAGTGGTGGGGCTCAACACACACTGGGGCC2160.17304225916282798No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAATAGTCT2150.17224113759262968No Hit
CAGGAAACAGCTATGACCGCAGCCACCCTGAACACACTTGGCTATCACCC2120.16983777288203486No Hit
CAGGAAACAGCTATGACCTGGGTTGCCTGGGACTGAGACAGCCACAGTAG1960.15701982775886242No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCACCAGCACAGGG1890.15141197676747448No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTATTGCAGCCTGT1890.15141197676747448No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACGGCCCCTCCGCC1870.14980973362707792No Hit
CAGGAAACAGCTATGACCTGGGTTGCCTGGGACTGAGAACAGGGACTGCT1820.1458041257760865No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCCTCTCCCAACT1820.1458041257760865No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCTAAACCAG1760.14099739635489686No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCCGACAGGGACGC1750.1401962747846986No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGTGGTGGGCCACA1700.13619066693370718No Hit
CAGGAAACAGCTATGACCCTGGGCCCCAAAGCAAAGGAGTAGGTGACGAG1610.12898057280192268No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACAGGGAGCTGCTGG1600.12817945123172442No Hit
CAGGAAACAGCTATGACCTCCCTCATTGTTGAGAGGCAGCCGTCGTTTTA1540.12337272181053476No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACACAGGCCACAGCC1470.1177648708191468No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCCAACACCCTTG1450.11616262767875024No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG1450.11616262767875024No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTCAGACTCACAC1440.11536150610855196No Hit
CAGGAAACAGCTATGACCTGCTGGGAGAGGGGGATGAGCGGCGCCGCCAT1440.11536150610855196No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCTTTCCTCCAGG1400.11215701982775887No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACCCAGGAGGGTCT1380.11055477668736231No Hit
CAGGAAACAGCTATGACCCCCCTCCGCTGGTTGAATGGCAGGAAACAGCT1370.10975365511716403No Hit
CAGGAAACAGCTATGACCTGACTGCAAACATAGAAGTGAGCAGAGCAAGA1350.10815141197676748No Hit
CAGGAAACAGCTATGACCCGGTGAGGTGGAGGGAGTCAGGACTGGCCGTC1350.10815141197676748No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACCATCCTGCCTCC1340.1073502904065692No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGCTGAATAGTTGT1340.1073502904065692No Hit
CAGGAAACAGCTATGACCTCCTGCCCCACCCTGATACAATATAGTCTCCC1310.10494692569597436No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCCCTTCCTTACCT1290.10334468255557781No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACATTCCAGAAGCC1280.10254356098537953No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTAACGCCTGTAA1260.10094131784498298No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGCGGCGCTCCCTG1260.10094131784498298No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACTAGAAGTAATTA1260.10094131784498298No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACCGCAGGTCCTTCT1250.1001401962747847No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)