FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003246366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183116
Sequences flagged as poor quality0
Sequence length300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG39942.1811310863059483No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA38162.083924943751502No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC35851.957775399200507No Hit
CAGGAAACAGCTATGACCGGGACCAGTCCAATAACCCCTGAGGGGTATAT32551.7775617641276567No Hit
CAGGAAACAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAACT32231.760086502544835No Hit
CAGGAAACAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAATT32001.7475261582821817No Hit
CAGGAAACAGCTATGACCGGGGAGACTGGGCCATACGATAGGCTTAATCC28621.5629437078136263No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC27231.487035540313244No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA25491.3920138054566504No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA25281.3805456650429235No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA24881.3587015880643962No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG24251.3242971668232157No Hit
CAGGAAACAGCTATGACCCATGGTTGGGGAAAAACTCCTCATCTTCATGT23781.2986303763734464No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCAGGAAACAGCT23521.2844317263374034No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC22561.2320059415889382No Hit
CAGGAAACAGCTATGACCCATGGTTGGGGAAAAACTCCTCATTTTCATGT21271.1615587933331877No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC20691.1298848817143232No Hit
CAGGAAACAGCTATGACCTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT20251.1058563970379431No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG18631.0173878852749076No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG18581.0146573756525918No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT17980.981891260184801No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG17810.9726075274689269No Hit
CAGGAAACAGCTATGACCTCCCACCACTGACTCCAGGGGAAGGAGGAGGA17110.934380392756504No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC16200.8846851176303546No Hit
CAGGAAACAGCTATGACCCCCTGCGGCTCCTTTCAAGTGCAGAGGAAGCC15690.8568339194827322No Hit
CAGGAAACAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG15500.8464579829179318No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA15490.8459118809934687No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC15150.8273444155617204No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC14900.813691867450141No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCTGGGTGCTGGTG14900.813691867450141No Hit
CAGGAAACAGCTATGACCGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA14600.7973088097162455No Hit
CAGGAAACAGCTATGACCAGGTTGCTGGATTCAGTTTGCTCAGGAAACAG14200.7754647327377182No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG14090.7694576115686231No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGTGGGGACTGGAA13840.7558050634570437No Hit
CAGGAAACAGCTATGACCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG13470.7355992922519059No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG13130.7170318268201578No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT12370.6755280805609559No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA12160.6640599401472291No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA11630.6351165381506805No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC11560.6312938246794382No Hit
CAGGAAACAGCTATGACCTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT11420.6236483977369536No Hit
CAGGAAACAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG11070.6045348303807423No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGCTCCTCCTCGC10690.5837829572511414No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG10680.5832368553266781No Hit
CAGGAAACAGCTATGACCAAACTATTAATACCTGCAGCAAAGCCCCAAGC10590.5783219380065096No Hit
CAGGAAACAGCTATGACCGGGCATGGTGTGGGACTAGCTAATCTAGGGAC10400.567946001441709No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC10320.5635771860460036No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCTGCCCAACACCC10160.5548395552545927No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT10040.5482863321610345No Hit
CAGGAAACAGCTATGACCTGGCATCCAGGGCCTATTTGGTTCCTATTGAG9890.5400948032940868No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT9830.5368181917473077No Hit
CAGGAAACAGCTATGACCCTTGGTGGAGCCATAGGCCGCATGCACGCGCA9800.5351798859739182No Hit
CAGGAAACAGCTATGACCTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG9440.5155202166932435No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGACACTGCCCACC9430.5149741147687804No Hit
CAGGAAACAGCTATGACCCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA9170.5007754647327377No Hit
CAGGAAACAGCTATGACCTGACAGCACAAGGAACACTGACCTACAGATAA8870.48439240699884223No Hit
CAGGAAACAGCTATGACCAGGGCAAGTGTTCGCGTTGTAGGCGGCGGGAC8740.4772930819808209No Hit
CAGGAAACAGCTATGACCCAGGGTGACTCCGATCCTGGAGCCAACGGTGC8350.45599510692675677No Hit
CAGGAAACAGCTATGACCGCATCCTGCTTAGTGCTGCCTCAATCTGGTCC8290.4527184953799777No Hit
CAGGAAACAGCTATGACCGTGCTCCTTGGGGACGTAGTCGCCTGGGGACT8260.4510801896065882No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT8230.4494418838331986No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG7870.4297822145525241No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCCGTCCTCGGAC7580.41394525874309185No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGGACACTGCCCAC7480.40848423949845997No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT7040.38445575482208No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTCACACCTGTAA6830.37298761440835315No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACTGGCGCCTCCCC6820.37244151248389No Hit
CAGGAAACAGCTATGACCTGCTGGGAGAGGGGGATGAGTAGACAGGGCCA6670.3642499836169423No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCGTGAGCCTCTGG6670.3642499836169423No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTCACGCCTGTAA6560.35824286244784725No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCTTCTGGGGCATC6560.35824286244784725No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTGTAATCCCAGC5810.3172852181131086No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTCTGCAGGTCTC5600.3058170776993818No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGGTACCCGTGTC5510.3009021603792132No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA5440.29707944690797095No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGACCTCTCCTCCT5380.2938028353611918No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGGCTGCTGCGAGT5210.2845191026453177No Hit
CAGGAAACAGCTATGACCCCCTGCGGCTCCTTTCAAGTTAAGTCACCACT5000.2730509622315909No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGCTGGGGACTGGA4990.2725048603071277No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCATA4990.2725048603071277No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACCCCGTTAGCTGC4870.2659516372135695No Hit
CAGGAAACAGCTATGACCTCCCTTAAACTTGGGATTGCTCCATTGCACTC4850.2648594333646432No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTGACACA4720.2577601083466218No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCTATTTTCCAAAG4720.2577601083466218No Hit
CAGGAAACAGCTATGACCAGGGCAAGTGTTCGCGTTGTGACAGTTACGGT4570.24956857947967406No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGGTGGGGACTGGA4570.24956857947967406No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGCGATCTCGTCTC4530.24738417178182137No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA4470.24410756023504226No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGTATAAAGTTGGA4410.24083094868826319No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCAGGTCTAGAGAA4310.2353699294436314No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCGGGTCTTACA4280.2337316236702418No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACGGGGATGGCAGTG4220.23045501212346273No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTTGGCTGGCTTG4170.22772450250114679No Hit
CAGGAAACAGCTATGACCACCTGGCAGCCCCAAATCTGCACCTCCCGCAG4140.22608619672775726No Hit
CAGGAAACAGCTATGACCTGCTTTGGGGCCATGGAGTTGGGGGAATGGGA4120.2249939928788309No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAAGGCCCCATCCGG4040.22062517748312546No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCACGGGCATCCTG4030.22007907555866227No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAAGGCCGCAGCTCC3990.21789466786080952No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT3960.21625636208742No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCTGAGCAGTGGGG3890.21243364861617772No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC3410.186220756241945No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGTGGTGGGCCACA3300.18021363507285001No Hit
CAGGAAACAGCTATGACCACCTGGCAGCCCCAAATCTGCACCTCCCGCGG3210.17529871775268135No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC3170.17311431005482864No Hit
CAGGAAACAGCTATGACCGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG3140.17147600428143908No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGCTGAATAGTTGT3110.16983769850804953No Hit
CAGGAAACAGCTATGACCTCCACAAGGCCCTGGGACAATCTCCCAGGCCC3010.16437667926341773No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGTGGACTAGGTCC2860.15618515039647No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAACCCCTGAGAACC2840.15509294654754363No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT2800.1529085388496909No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGCCTGGCTTGTC2770.15127023307630136No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCAAGAGGGGGCAG2770.15127023307630136No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT2760.15072413115183816No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCGTGCTGGGCCA2710.14799362152952228No Hit
CAGGAAACAGCTATGACCGGCGGGCCCTGATATAGAGCCACTGGCCGTCG2680.14635531575613273No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTATGACCGCCCACC2610.14253260228489045No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTATAATCCCAGC2600.14198650036042726No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCCATGCTCACCT2570.14034819458703773No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAACAGGCCCCCGCA2460.13434107341794274No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTGAGGAGGGTGG2450.13379497149347955No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAACACGCAGCTCCC2420.13215666572009No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCCGGCATTGTAGT2170.11850411760851046No Hit
CAGGAAACAGCTATGACCAACACCCTTGGCCTCCCTCACTGGCCGTCGTT2170.11850411760851046No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCCTGGGTGTCTG2130.11631970991065772No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCAGGGCAGCGGT2120.11577360798619456No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG2110.11522750606173136No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCAAGTGCTGATGTA2100.11468140413726818No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTCACACCTATAA2070.11304309836387863No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCATGTGTTCAGC2060.11249699643941545No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGATGCTGCCTCCT1970.10758207911924682No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGACACTGGCAGGT1880.10266716179907819No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTAGGGTCTGGGGC1860.10157495795015183No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTCACGTCTGTAA1860.10157495795015183No Hit
CAGGAAACAGCTATGACCTGACAGCACAAGGAACACTGACCCGAGGCAGG1840.10048275410122545No Hit
CAGGAAACAGCTATGACCTGACTGCAAACATAGAAGTGAGCAGAGCAAGA1840.10048275410122545No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)