FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155381
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAANGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA35842.306588321609463No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA33052.12702968831453No Hit
TGTAAAANGACGGCCAGTTCCCAAAGGCTGGTGGAGGAGATGCTGAAGAT29291.8850438599313943No Hit
TGTAAAANGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG27251.7537536764469273No Hit
TGTAAAANGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC27031.7395949311691905No Hit
TGTAAAACGACGGCCAGTTCCCAAAGGCTGGTGGAGGAGATGCTGAAGAT25531.6430580315482588No Hit
TGTAAAANGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT25471.6391965555634214No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG24561.5806308364600563No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC24551.57998725712925No Hit
TGTAAAANGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA23201.4931040474704114No Hit
TGTAAAANGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC22241.431320431713015No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA22151.425528217735759No Hit
TGTAAAACGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT21981.4145873691120536No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC19851.2775049716503304No Hit
TGTAAAANGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC19631.2633462263725939No Hit
TGTAAAANGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC19001.2228007285318025No Hit
TGTAAAANGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA18941.2189392525469653No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC18281.1764630167137553No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA17561.130125304895708No Hit
TGTAAAANGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG16901.087649069062498No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC16511.0625494751610558No Hit
TGTAAAANGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC15991.0290833499591328No Hit
TGTAAAANGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT15891.0226475566510704No Hit
TGTAAAANGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT15881.0220039773202645No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG15230.9801713208178606No Hit
TGTAAAANGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT15210.9788841621562482No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT14330.9222491810453015No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC14150.9106647530907899No Hit
TGTAAAANGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG13500.868832096588386No Hit
TGTAAAACGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT13460.866257779265161No Hit
TGTAAAANGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC13440.8649706206035488No Hit
TGTAAAACGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT13420.8636834619419362No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC12360.7954640528764777No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG12240.7877411009068034No Hit
TGTAAAANGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC12240.7877411009068034No Hit
TGTAAAANGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG10980.7066501052252206No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC10970.7060065258944144No Hit
TGTAAAANGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG10900.7015014705787709No Hit
TGTAAAANGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT10670.686699145970228No Hit
TGTAAAANGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC10380.6680353453768478No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT9910.637787116828956No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG9850.6339256408441187No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG9660.6216976335588006No Hit
TGTAAAANGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG8890.5721420250867223No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC8780.565062652447854No Hit
TGTAAAANGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC8710.5605575971322105No Hit
TGTAAAANGACGGCCAGTTCTTCACCTCCCAAAATGCTGGGATTACAGGC8390.5399630585464117No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC8320.5354580032307682No Hit
TGTAAAANGACGGCCAGTTCCTGGGCTTCTCTTGAGTACGGAGGAAAGTG8200.5277350512610938No Hit
TGTAAAANGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC8010.5155070439757756No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG7670.4936253467283645No Hit
TGTAAAANGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG7320.47110007015014704No Hit
TGTAAAACGACGGCCAGTTCCTGGGCTTCTCTTGAGTACGGAGGAAAGTG7090.4562977455416042No Hit
TGTAAAANGACGGCCAGTTCCATTACTCTACACAAGCAGGGTGAAGGTGA7040.45307984888757313No Hit
TGTAAAANGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC7000.45050553156434825No Hit
TGTAAAACGACGGCCAGTTCTTCACCTCCCAAAATGCTGGGATTACAGGC6980.44921837290273586No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC6800.43763394494822405No Hit
TGTAAAACGACGGCCAGTTCCATTACTCTACACAAGCAGGGTGAAGGTGA6680.4299109929785495No Hit
TGTAAAANGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC5680.3655530598979283No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC5360.34495852131212956No Hit
TGTAAAACGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG5340.3436713626505171No Hit
TGTAAAANGACGGCCAGTCAGCAGCAACTCCCTGTGCTCCCTTCTCTTTG4820.31020523744859413No Hit
TGTAAAANGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC4670.3005515474865009No Hit
TGTAAAACGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC4660.2999079681556947No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTCCCTTCTCTTTG4590.2954029128400512No Hit
TGTAAAACGACGGCCAGTGCCATCGGCTCCCAAGTTTTATGTCCTAGAGC4440.28574922287795806No Hit
TGTAAAANGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGTGAACGAAG4420.2844620642163456No Hit
TGTAAAANGACGGCCAGTGCCATCGGCTCCCAAGTTTTATGTCCTAGAGC4270.2748083742542525No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC4190.26965973960780276No Hit
TGTAAAANGACGGCCAGTAAACCTGGCCCCACTGCCCCTGCACATGCAAG4170.2683725809461903No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCCCTGCACATGCAAG3640.23426287641346108No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGTGAACGAAG3400.21881697247411203No Hit
TGTAAAANGACGGCCAGTGGTACGTGGTGGTGGCTGAGGAGCTCAAGGGT3370.2168862344816934No Hit
TGTAAAANGACGGCCAGTGCCCAAGGTTGGTGAGATGGGAACCACCCAGC3240.20851970318121263No Hit
TGTAAAANGACGGCCAGTGATCCAAGGCTCCTCGTCCGTGTAAAACGACG3040.1956481165650884No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGAGCTCAAGGGT2940.18921232325702628No Hit
TGTAAAANGACGGCCAGTTGAAGAATGGTGGCTTGTTTCATGGTGTAAAA2940.18921232325702628No Hit
TGTAAAANGACGGCCAGTCAACCTCAGGCGATCCACCCGCCTCGGCCTCC2900.18663800593380142No Hit
TGTAAAANGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG2900.18663800593380142No Hit
TGTAAAANGACGGCCAGTCGGTGCTCTGGTGAGATGGGGGGCCCGGATGT2850.18342010927977037No Hit
TGTAAAACGACGGCCAGTGCCCAAGGTTGGTGAGATGGGAACCACCCAGC2820.18148937128735174No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGTGTAAAACGACG2790.1795586332949331No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCTCAGCCTTCTGGGTC2750.17698431597170824No Hit
TGTAAAACGACGGCCAGTTGAAGAATGGTGGCTTGTTTCATGGTGTAAAA2620.1686177846712275No Hit
TGTAAAANGACGGCCAGTAAACCTGGCCCCACTGCTCAGCCTTCTGGGTC2550.16411272935558402No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG2540.1634691500247778No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCGCCTCGGCCTCC2520.16218199136316538No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGGGGCCCGGATGT2350.15124114273945977No Hit
TGTAAAANGACGGCCAGTTGAAGAATGGTGGCTTGTTTCATGGTGGCCTT2340.15059756340865357No Hit
TGTAAAANGACGGCCAGTAGTGGAAGCTATTAGCACCAATGACATAAATA2310.14866682541623494No Hit
TGTAAAACGACGGCCAGTAGTGGAAGCTATTAGCACCAATGACATAAATA2150.13836955612333554No Hit
TGTAAAANGACGGCCAGTGTCCCAGGTGTATGGAGCGCCACATCTCCTGA2070.13322092147688586No Hit
TGTAAAANGACGGCCAGTACCTGAATCTTACTAAGCGCCTGGCGGTCATA2040.13129018348446722No Hit
TGTAAAANGACGGCCAGTTCCCACCACCATACCCCAACTTAGTATTTGAA2030.130646604153661No Hit
TGTAAAANGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGCTACGGGGT2020.1300030248228548No Hit
TGTAAAACGACGGCCAGTTGAAGAATGGTGGCTTGTTTCATGGTTGTAAA2020.1300030248228548No Hit
TGTAAAACGACGGCCAGTTGAAGAATGGTGGCTTGTTTCATGGTGGCCTT2020.1300030248228548No Hit
TGTAAAANGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT2020.1300030248228548No Hit
TGTAAAANGACGGCCAGTACCTGAATCTTACTAAGCGCCTGGTGTAAAAC1880.12099291419156782No Hit
TGTAAAANGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG1850.11906217619914919No Hit
TGTAAAANGACGGCCAGTTGAAGAATGGTGGCTTGTTTCATGGTTGTAAA1840.11841859686834298No Hit
TGTAAAANGACGGCCAGTAGTACTCCCTCCTGCCTGTGGTCCCAGCTACT1830.11777501753753676No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGTCCCAGCTACT1780.11455712088350571No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGCTACGGGGT1770.11391354155269949No Hit
TGTAAAANGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGCGCCTG1760.11326996222189328No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT1730.11133922422947465No Hit
TGTAAAACGACGGCCAGTACCTGAATCTTACTAAGCGCCTGGCGGTCATA1710.11005206556786223No Hit
TGTAAAACGACGGCCAGTACCTGAATCTTACTAAGCGCCTGGTGTAAAAC1680.1081213275754436No Hit
TGTAAAACGACGGCCAGTTCCCACCACCATACCCCAACTTAGTATTTGAA1660.10683416891383117No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCCACATCTCCTGA1640.10554701025221873No Hit
TGTAAAANGACGGCCAGTACACATTGAGAGCATCAGTGGGGCACATGGGT1610.1036162722598001No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGCACATGGGT1600.1029726929289939No Hit
TGTAAAANGACGGCCAGTAAACCTGGCCCCACTGCCCTGCCTCTCCCAGG1580.10168553426738147No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)