FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111025
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT23582.123845980634992No Hit
CAGGAAACAGCTATGACCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT22392.0166629137581626No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC21851.9680252195451473No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT21381.9256924116190048No Hit
CAGGAAACAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG19751.778878630938978No Hit
CAGGAAACAGCTATGACCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC17951.616752983562261No Hit
CAGGAAACAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAATT16931.524881783382121No Hit
CAGGAAACAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAACT15301.3780680027020942No Hit
CAGGAAACAGCTATGACCGGGGAGACTGGGCCATACGATAGGCTTAATCC14171.2762891240711551No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT13731.2366584102679576No Hit
CAGGAAACAGCTATGACCGGGACCAGTCCAATAACCCCTGAGGGGTATAT13671.231254222022067No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT13261.194325602341815No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCTGAGGCAGGAGA13071.1772123395631615No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGGACACTGCCCAC12361.1132627786534564No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG12231.1015537041206935No Hit
CAGGAAACAGCTATGACCCATGGTTGGGGAAAAACTCCTCATCTTCATGT11741.0574195001125872No Hit
CAGGAAACAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT11321.0195901823913534No Hit
CAGGAAACAGCTATGACCCATGGTTGGGGAAAAACTCCTCATTTTCATGT10830.975455978383247No Hit
CAGGAAACAGCTATGACCTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT10700.9637469038504841No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA10440.9403287547849584No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG10200.918712001801396No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC10030.9034001351047061No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC9670.8709750056293628No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA9580.862868723260527No Hit
CAGGAAACAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT8850.7971177662688584No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA8660.7800045034902049No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTCCCCAACCATAG8270.7448772798919162No Hit
CAGGAAACAGCTATGACCAGAGGCACAGGTGGAGAGGGACGAGACCCTGA7630.6872326052690835No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC7470.6728214366133753No Hit
CAGGAAACAGCTATGACCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG7450.6710200405314118No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC7100.6394956090970503No Hit
CAGGAAACAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT6920.6232830443593785No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG6740.6070704796217069No Hit
CAGGAAACAGCTATGACCTCCCACCACTGACTCCAGGGGAAGGAGGAGGA6490.5845530285971628No Hit
CAGGAAACAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG6260.5638369736545823No Hit
CAGGAAACAGCTATGACCGGCTGGCACAAAGAACAGCACTGACTGTTTAT6140.5530285971628012No Hit
CAGGAAACAGCTATGACCCTTGGTGGAGCCATAGGCCGCATGCACGCGCA6130.5521278991218195No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC6050.5449223147939654No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA6030.5431209187120017No Hit
CAGGAAACAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG5990.5395181265480747No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGTCTTTGGGGACT5800.5224048637694213No Hit
CAGGAAACAGCTATGACCAGACAGGGTCTTGCTATGTTGCCCAGGCTAGA5720.5151992794415672No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACACAGCTGGGAGGC5670.5106957892366584No Hit
CAGGAAACAGCTATGACCTGAAGTGGCTCCATCCTTCCATTTGTTTATAT5530.49808601666291374No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT5520.49718531862193205No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT5510.49628462058095024No Hit
CAGGAAACAGCTATGACCCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA5440.48997973429407793No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT5440.48997973429407793No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGACACTGCCCACC5370.4836748480072056No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT5280.47556856563836974No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGCACAGACCCCACA5120.46115739698266156No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACGGGGATGGCAGTG5110.4602566989416798No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT4930.4440441342040081No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA4920.44314343616302637No Hit
CAGGAAACAGCTATGACCCCAGGAGAGAGCAGCCTTCCTCACACCCGTGA4910.44224273812204457No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA4880.43954064399909937No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG4740.42693087142535463No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT4540.40891691060571944No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGAGCCT4530.4080162125647377No Hit
CAGGAAACAGCTATGACCAAACTATTAATACCTGCAGCAAAGCCCCAAGC4430.39900923215492No Hit
CAGGAAACAGCTATGACCAGGGCAAGTGTTCGCGTTGTAGGCGGCGGGAC4430.39900923215492No Hit
CAGGAAACAGCTATGACCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG4220.3800945732943031No Hit
CAGGAAACAGCTATGACCTGCCCAGTTGTTTCCAGAACCTTGTTTTTGAC4090.3683854987615402No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGTCTGGGTCCT3900.35127223598288676No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGCAGGAAACAGCT3890.35037153794190495No Hit
CAGGAAACAGCTATGACCTGACAGCACAAGGAACACTGACCTACAGATAA3770.3395631614501239No Hit
CAGGAAACAGCTATGACCTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG3530.3179464084665616No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGCAGGAAACAGCT3430.30893942805674396No Hit
CAGGAAACAGCTATGACCACCTGGCAGCCCCAAATCTGCACCTCCCGCGG3370.30353523981085345No Hit
CAGGAAACAGCTATGACCGGCAGACAGCCTCTCCTCCTTTCATCACAAAA3320.29903174960594464No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCACAGGAAACAGC3130.28191848682729115No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGGCTCTTTT3130.28191848682729115No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCCAGCTAAGTGA2960.2666066201306012No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG2950.26570592208961946No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC2890.2603017338437289No Hit
CAGGAAACAGCTATGACCGCATCCTGCTTAGTGCTGCCTCAATCTGGTCC2880.25940103580274715No Hit
CAGGAAACAGCTATGACCCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG2860.2575996397207836No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCACTCAGCGTGTG2710.24408916910605719No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCCCTCGGGCAG2640.23778428281918487No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGTCTGCAGCCTGG2490.22427381220445847No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC2430.2188696239585679No Hit
CAGGAAACAGCTATGACCTGCAATCTTGAGCTCCTGGGCCGTCGTTTTAC2380.2143661337536591No Hit
CAGGAAACAGCTATGACCCCCTGCGGCTCCTTTCAAGTTAAGTCACCACT2340.21076334158973203No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG2240.20175636117991444No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCCAAACCAG2140.19274938077009685No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG2100.18914658860616979No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCACGGTGGCTCAT2080.18734519252420626No Hit
CAGGAAACAGCTATGACCGGGACCAGTCCAATAACCCCTGACAGGAAACA2060.18554379644224275No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC2040.18374240036027922No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT2020.1819410042783157No Hit
CAGGAAACAGCTATGACCTCCACAAGGCCCTGGGACAATCTCCCAGGCCC2010.18104030623733391No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC1980.17833821211438866No Hit
CAGGAAACAGCTATGACCACCTGGCAGCCCCAAATCTGCACCTCCCGCAG1980.17833821211438866No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAACCATGTGCCC1890.1702319297455528No Hit
CAGGAAACAGCTATGACCAGAGGCACAGGTGGAGAGGGGTGATGGCCATG1720.15492006304886285No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGGGGGCCTGGCT1660.14951587480297232No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGCTTGGGCAGGT1630.14681378068002704No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC1630.14681378068002704No Hit
CAGGAAACAGCTATGACCACCTTTACCCAAATAGCTACCACCACATCTTG1600.14411168655708173No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGCCTTGGAGCTCGG1590.14321098851609998No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCTATTTTCCAAAG1590.14321098851609998No Hit
CAGGAAACAGCTATGACCTGCAATCTTGAGCTCCTGGGCTCAGGTGATCT1570.14140959243413645No Hit
CAGGAAACAGCTATGACCTTCCTGATGGGATTCAGCCATCAACAGTAGGA1570.14140959243413645No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACTGCTAGGAGGGTG1550.13960819635217295No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA1470.13240261202431886No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTAGCCCACCGCAAA1440.12970051790137357No Hit
CAGGAAACAGCTATGACCGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC1430.1287998198603918No Hit
CAGGAAACAGCTATGACCAGACAGGGTCTTGCTATGTTGCCCAGGCTGGT1430.1287998198603918No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCGCAGTGGCTGAT1430.1287998198603918No Hit
CAGGAAACAGCTATGACCTTGGACAGCACCTTGAATGCTTTCAGGGAAGC1430.1287998198603918No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGCCTCCGGGGATCA1420.12789912181941004No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCAGGCAAACTTGG1420.12789912181941004No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCAGAGAGTGATAT1420.12789912181941004No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCCTCCCCACCTCC1410.12699842377842827No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT1360.12249493357351948No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGTGGTGGGCCACA1350.12159423553253772No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGCGGCCTTGCTGA1300.11709074532762892No Hit
CAGGAAACAGCTATGACCACCACACCTGGCCTGAGGATGGTACACGTCGG1270.11438865120468364No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCTAAACCAG1260.11348795316370187No Hit
CAGGAAACAGCTATGACCTCCTGCCCCACCCTGATACAATATAGTCTCCC1240.11168655708173834No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTGATCCCCCAAAA1240.11168655708173834No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCATGTGTTCAGC1230.11078585904075659No Hit
CAGGAAACAGCTATGACCCTCTTCCTGGGGGCTACAACTGGCCGTCGTTT1160.10448097275388427No Hit
CAGGAAACAGCTATGACCAGACAGGGTCTTGCTATGTTGCCTGGGCTGGT1140.10267957667192075No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCGGGTCTTACA1140.10267957667192075No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCCTGCCCCCTGCA1130.10177887863093897No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCCAACACCACTGG1120.10087818058995722No Hit
CAGGAAACAGCTATGACCGCAGCCACCCTGAACACACTTGGCTATCACCC1120.10087818058995722No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)