FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003246382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189699
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG56512.9789297782276134No Hit
CAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT53632.8271103168704106No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC33971.7907316327445058No Hit
CAGGAAANAGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC22801.2019040690778549No Hit
CAGGAAANAGCTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC22311.176073674610831No Hit
CAGGAAANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT21371.126521489306744No Hit
CAGGAAANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG20521.0817136621700694No Hit
CAGGAAANAGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT20431.076969304002657No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT19631.034797231403434No Hit
CAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC12040.6346896926183059No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT11900.6273095799134418No Hit
CAGGAAANAGCTATGANNGGGGAGACTGGGCCATACGATAGGCTTAATCC11740.6188751653935972No Hit
CAGGAAANAGCTATGANNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT11330.5972619781864954No Hit
CAGGAAANAGCTATGANNCCTTGGGCAAGTTAACAAACCTCTCTGAGCCT11000.579865998239316No Hit
CAGGAAANAGCTATGANNAGCGTCTCATTCCATAAAGGCCACCATGAAAC10950.5772302437018645No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT9570.5044834184682049No Hit
CAGGAAANAGCTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA9310.49077749487345745No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC9220.48603313670604487No Hit
CAGGAAANNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC9050.4770715712787099No Hit
CAGGAAANAGCTATGANNAGAGGCACAGGTGGAGAGGGACGAGACCCTGA8450.4454425168292927No Hit
CAGGAAANNGCTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC8120.42804653688211325No Hit
CAGGAAANAGCTATGANNTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG8080.42593793325215207No Hit
CAGGAAANAGCTATGANNTGCCCAGTTGTTTCCAGAACCTGAATCCACAT7660.40379759513756No Hit
CAGGAAANAGCTATGANNCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT7600.4006346896926183No Hit
CAGGAAANNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT7320.3858744642828903No Hit
CAGGAAANAGCTATGANNAGAGACCAGATGCATAAGCACAGCAGCATTCC7000.36900563524320107No Hit
CAGGAAANNGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT6880.36267982435331764No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC6280.3310507699039004No Hit
NAGGANANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG6120.3226163553840558No Hit
CAGGANANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG6080.3205077517540946No Hit
CAGGAAANAGCTATGANNCTGGCCTCCTTCAATGCATTTAATGGCCTTCT6060.31945344993911406No Hit
CAGGAAANAGCTATGANNCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC6050.3189262990316238No Hit
NAGGAAANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG6030.3178719972166432No Hit
NAGGAAANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT6030.3178719972166432No Hit
CAGGANANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT5720.30153031908444433No Hit
NAGGANANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT5670.29889456454699287No Hit
CAGGAAANAGCTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA5630.29678596091703174No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC5450.28729724458220657No Hit
CAGGAAANAGCTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT5400.2846614900447551No Hit
CAGGAAANAGCTATGANNATCGCACCACTGCACTCCAGCAGGAAACAGCT5350.28202573550730364No Hit
CAGGAAANAGCTATGANNGGAGGCTGAGGTGGGAGGATCACTTGAGCCTG5310.2799171318773425No Hit
CAGGAAANAGCTATGANNCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG5230.2756999246174202No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT4970.2619940010226728No Hit
CAGGAAANAGCTATGANNTGACAGCACAAGGAACACTGACCGTCGTTTTA4960.26146685011518245No Hit
CAGGAAANAGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT4940.2604125483002019No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC4780.25197813378035727No Hit
CAGGAAANNGCTATGANNAGCGTCTCATTCCATAAAGGCCACCATGAAAC4630.244070870168003No Hit
CAGGAAANAGCTATGANNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT4440.23405500292568754No Hit
CAGGAAANNGCTATGANNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT4440.23405500292568754No Hit
CAGGAAANNGCTATGANNCCTTGGGCAAGTTAACAAACCTCTCTGAGCCT4260.22456628659086236No Hit
CAGGAAANAGCTATGANNTGCCCAGTTGTTTCCAGAACCTTGTTTTTGAC4260.22456628659086236No Hit
CAGGAAANNGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT4160.21929477751595952No Hit
CAGGAAANAGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG4120.21718617388599834No Hit
CAGGAAANNGCTATGANNGGGGAGACTGGGCCATACGATAGGCTTAATCC3900.20558885392121204No Hit
CAGGAAACAGCNATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG3870.20400740119874117No Hit
CAGGAAANAGCTATGANNGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG3840.20242594847627032No Hit
CAGGAAANNGCTATGANNAGAGGCACAGGTGGAGAGGGACGAGACCCTGA3750.19768159030885774No Hit
NAGGANANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC3700.19504583577140627No Hit
NAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC3630.19135577941897425No Hit
CAGGANANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC3560.18766572306654225No Hit
CAGGAAANNGCTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA3530.1860842703440714No Hit
CAGGAAANNGCTATGANNTGCCCAGTTGTTTCCAGAACCTGAATCCACAT3520.1855571194365811No Hit
NAGGAAANAGCTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG3510.18502996852909082No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTATGTGCA3480.18344851580661994No Hit
CAGGAAANAGCTATGANNGGAGGCTGAGGTGGGAGGATCACCTGAGCCTG3440.18133991217665882No Hit
CAGGAAACAGCTATGANCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT3390.17870415763920736No Hit
CAGGAAACNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT3280.1729054976568142No Hit
CAGGAAANAGCTATGANNTGACAGCACAAGGAACACTGACCTACAGATAA3270.17237834674932392No Hit
CAGGAAACAGCNATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT3210.1692154413043822No Hit
CAGGAANNNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT3190.16816113948940165No Hit
CAGGAAANAGCTATGANNCCAGGAGAGAGCAGCCTTCCTCACACCCGTGA3180.16763398858191134No Hit
CAGGAAANAGCTATGANNGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG3170.16710683767442105No Hit
CAGGAAANNGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT3160.16657968676693077No Hit
NAGGAAANAGCTATGANCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT3160.16657968676693077No Hit
CAGGAAACNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG3150.16605253585944046No Hit
CAGGAANNNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG3110.16394393222947934No Hit
CAGGAAANNGCTATGANNCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT3010.15867242315457647No Hit
CAGGAAANAGCTATGANNTGAAGTGGCTCCATCCTTCCATTTGTTTATAT2950.15550951770963473No Hit
NAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2900.15287376317218332No Hit
CAGGAAANNGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC2890.152346612264693No Hit
NAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG2850.15023800863473186No Hit
CAGGAAANNGCTATGANNAGAGACCAGATGCATAAGCACAGCAGCATTCC2840.14971085772724158No Hit
CAGGAAACAGCTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG2820.148656555912261No Hit
CAGGAAANAGCTATGANNATCGCACCACTGCGCTCCATACACCTGGGACA2800.14760225409728042No Hit
CAGGAAANNGCTATGANNTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG2770.14602080137480958No Hit
NAGGAAANNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2750.144966499559829No Hit
CAGGAAANAGCTATGANNGCTTCTCGGCCATGTGGGTTAAACTCGGGGAT2700.14233074502237755No Hit
CAGGAAANAGCTATGANNCCAGGAGAGAGCAGCCTTCCTGTGTACTCAGA2600.13705923594747468No Hit
CAGGAAANNGCTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA2580.13600493413249412No Hit
CAGGAAANAGCTATGANNGGAGGCTGAGGTGGGAGGATCACTTGAGCCCA2440.1286248214276301No Hit
CAGGAAANNGCTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT2370.12493476507519807No Hit
NAGGANANNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2360.12440761416770778No Hit
NAGGANANNGCTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC2330.12282616144523692No Hit
CAGGAAANAGCTATGANNTGCTGCCGAGAGAGGACAGTGATCCCCCAAAA2330.12282616144523692No Hit
CAGGAAANNGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC2310.12177185963025636No Hit
CAGGAAANNGCTATGANNCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC2300.12124470872276606No Hit
CAGGANANNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2280.1201904069077855No Hit
NAGGAAANNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT2280.1201904069077855No Hit
NAGGANANNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT2250.11860895418531463No Hit
CAGGAAANAGCTATGANNGGCAGACAGCCTCTCCTCCTTTCATCACAAAA2200.1159731996478632No Hit
NAGGAAANNGCTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC2180.11491889783288262No Hit
CAGGANANNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT2180.11491889783288262No Hit
CAGGAAACAGCNATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2170.11439174692539233No Hit
CAGGAAANNGCTATGANNATCGCACCACTGCACTCCAGCAGGAAACAGCT2160.11386459601790205No Hit
CAGGANANNGCTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC2140.11281029420292148No Hit
CAGGAAANNGCTATGANNCTGGCCTCCTTCAATGCATTTAATGGCCTTCT2130.11228314329543118No Hit
CAGGAAANNGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC2060.10859308694299917No Hit
CAGGAAANNGCTATGANNGGAGGCTGAGGTGGGAGGATCACTTGAGCCTG2050.1080659360355089No Hit
CAGGAAANNGCTATGANNTGACAGCACAAGGAACACTGACCGTCGTTTTA2020.10648448331303802No Hit
CAGGAAANAGCTATGANNAACACGGCAGTCAGAGGTGCAGGAAACAGCTA2020.10648448331303802No Hit
CAGGAAANAGCTATGANNGGAGGCTGAGGTGGGAGGATCACTTGACCCCC1900.10015867242315457No Hit
CAGGAAANNGCTATGANNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT1900.10015867242315457No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)