FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003246392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207880
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT11270.5421396959784491No Hit
NNGGANNNNGNTATGANNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT8180.3934962478352896No Hit
NNGGANNNNGNTATGNNNAAACCCTCGACCCTGAGGCTGGAAGCGCCCCA7770.37377333076775066No Hit
NNGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT7590.36511448912834327No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT7540.362709255339619No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC7510.36126611506638445No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAATT7350.35356936694246677No Hit
NNGGANNNNGNTATGNNNTCCACAAGGCCCTGGGACAAGGAAAATGGAGA6870.3304791225707139No Hit
NNGGANNNNGNTATGNNNCAGGGTGACTCCGATCCTGGAGCCAACGGTGC6510.31316143929189916No Hit
NNGGANNNNGNTATGNNNCCCTGCGGCTCCTTTCAAGTGCAGAGGAAGCC6490.3121993457764095No Hit
NNGGANNNNGNTATGNNNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC6370.30642678468347123No Hit
NNGGANNNNGNTATGNNNAGAGGCACAGGTGGAGAGGGACGAGACCCTGA6220.2992110833172984No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG6200.29824898980180875No Hit
NNGGANNNNGNTATGNNNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT6010.2891091014046565No Hit
NNGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT5880.28285549355397344No Hit
NNGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG5820.2799692130075043No Hit
NNGGANNNNGNTATGANNAAACCCTCGACCCTGAGGCTGGAAGCGCCCCA5710.27467769867231095No Hit
NNGGANNNNGNTATGANNTCCACAAGGCCCTGGGACAAGGAAAATGGAGA5510.2650567635174139No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT5510.2650567635174139No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC5380.2588031556667308No Hit
NNGGANNNNGNTATGNNNAGGGCAAGTGTTCGCGTTGTGACAGTTACGGT5330.25639792187800653No Hit
NNGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGGACACTGCCCAC5310.2554358283625169No Hit
NNGGANNNNGNTATGANNCCCTGCGGCTCCTTTCAAGTGCAGAGGAAGCC4930.23715605156821243No Hit
NAGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT4730.2275351164133154No Hit
NNGGANNNNGNTATGNNNGCTAATGCTTACTCTTTGCGTGAGTACTGGTT4700.2260919761400808No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT4620.222243602078122No Hit
NNGGANNNNGNTATGANNCAGGGTGACTCCGATCCTGGAGCCAACGGTGC4600.2212815085626323No Hit
NNGGANNNNGNTATGANNAGAGGCACAGGTGGAGAGGGACGAGACCCTGA4570.21983836828939773No Hit
NNGGANNNNGNTATGNNNACCTCTCTGGGCCTGTCTCCTCCCCGCCAAGA4480.21550894746969404No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG4320.2078121993457764No Hit
NNGGANNNNGNTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC4290.20636905907254185No Hit
NNGGANNNNGNTATGANNAGGGCAAGTGTTCGCGTTGTGACAGTTACGGT4120.19819126419087937No Hit
NNGGANNNNGNTATGNNNTGACTGAAAGGCTTTTCCACGTGCACTGCTGG4110.1977102174331345No Hit
NAGGANNNNGNTATGNNNAAACCCTCGACCCTGAGGCTGGAAGCGCCCCA4090.1967481239176448No Hit
NNGGANNNNGNTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC4070.19578603040215511No Hit
NAGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT4040.19434289012892053No Hit
NNGGANNNNGNTATGNNNGCTGCTGACCCAACCCTACCTCTGCAGCTAGC4020.19338079661343083No Hit
NNGGANNNNGNTATGNNNATCCACTGTTGGCCAACACACCTGGCCTTCAA3880.18664614200500287No Hit
NNGGANNNNGNTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG3860.1856840484895132No Hit
NNGGANNNNGNTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT3790.1823167211852992No Hit
NNGGANNNNGNTATGANNGCTAATGCTTACTCTTTGCGTGAGTACTGGTT3780.18183567442755436No Hit
NNGGANNNNGNTATGNNNCCTGGACGCAATCACCCAGGCTGCAGTGCGGT3670.17654416009236096No Hit
NNGGANNNNGNTATGNNNCCCAAACCTGCGGAGGCACACAGCTGGGAGGC3660.17606311333461613No Hit
NNGGANNNNGNTATGNNNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA3550.17077159899942274No Hit
NNGGANNNNGNTATGNNNACTCCCAACTCCCGTCTGGTCGGGGCAAAAAC3540.17029055224167788No Hit
NNGGANNNNGNTATGANNGCTGCTGACCCAACCCTACCTCTGCAGCTAGC3510.16884741196844333No Hit
NNGGANNNNGNTATGNNNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA3450.16596113142197422No Hit
NAGGANNNNGNTATGANNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT3410.1640369443909948No Hit
NNGGANNNNGNTATGANNACCTCTCTGGGCCTGTCTCCTCCCCGCCAAGA3330.160188570329036No Hit
NNGGANNNNGNTATGNNNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG3330.160188570329036No Hit
NAGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT3280.1577833365403117No Hit
NNGGANNNNGNTATGNNNCTGGAATTTCAACAGAATGGCATTTATATGAT3260.156821243024822No Hit
NNGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGACACTGCCCACC3250.15634019626707715No Hit
NNGGANNNNGNTATGNNNCCACCCTCATGCCTAACCCCAGCACAGGGAGT3200.1539349624783529No Hit
NAGGANNNNGNTATGNNNAGAGGCACAGGTGGAGAGGGACGAGACCCTGA3150.15152972868962863No Hit
NAGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC3130.15056763517413893No Hit
NAGGANNNNGNTATGNNNTCCACAAGGCCCTGGGACAAGGAAAATGGAGA3120.15008658841639408No Hit
NNGGANNNNGNTATGNNNTCCTGCCCCACCCTGATACAGGTCACTGTGGA3070.14768135462766982No Hit
NNGGANNNNGNTATGNNNGCATCCTGCTTAGTGCTGCCCACAGTGTCATT3040.14623821435443526No Hit
NNGGANNNNGNTATGANNTGACTGAAAGGCTTTTCCACGTGCACTGCTGG3040.14623821435443526No Hit
NAGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAATT2980.14335193380796613No Hit
NAGGANNNNGNTATGNNNCCCTGCGGCTCCTTTCAAGTGCAGAGGAAGCC2980.14335193380796613No Hit
NNGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATCCAAACCAG2920.14046565326149701No Hit
NNGGANNNNGNTATGNNNTCCCACCACTGACTCCAGGGGAAGGAGGAGGA2860.1375793727150279No Hit
NAGGANNNNGNTATGNNNAGGGCAAGTGTTCGCGTTGTGACAGTTACGGT2850.13709832595728305No Hit
NNGGANNNNGNTATGNNNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT2830.13613623244179335No Hit
NAGGANNNNGNTATGNNNCAGGGTGACTCCGATCCTGGAGCCAACGGTGC2830.13613623244179335No Hit
NNGGANNNNGNTATGANNCCTGGACGCAATCACCCAGGCTGCAGTGCGGT2770.1332499518953242No Hit
NNGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATCTAAACCAG2700.12988262459111025No Hit
NNGGANNNNGNTATGANNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG2700.12988262459111025No Hit
NAGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGGACACTGCCCAC2680.12892053107562057No Hit
NAGGANNNNGNTATGNNNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2670.1284394843178757No Hit
NNGGANNNNGNTATGANNCCCAAACCTGCGGAGGCACACAGCTGGGAGGC2660.12795843756013084No Hit
NAGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2640.12699634404464114No Hit
NNGGANNNNGNTATGANNATCCACTGTTGGCCAACACACCTGGCCTTCAA2550.12266692322493745No Hit
NNGGANNNNGNTATGNNNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA2530.12170482970944776No Hit
NNGGANNNNGNTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT2520.1212237829517029No Hit
NAGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2490.11978064267846834No Hit
NNGGANNNNGNTATGNNNCCACCCTCATGCCTAACCCCCGTCCTCCCAGC2450.11785645564748894No Hit
NNGGANNNNGNTATGANNACTCCCAACTCCCGTCTGGTCGGGGCAAAAAC2440.11737540888974408No Hit
NAGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG2430.11689436213199925No Hit
NNGGANNNNGNTATGNNNGGGCATGGTGTGGGACTAGCTAATCTAGGGAC2430.11689436213199925No Hit
NNGGANNNNGNTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA2370.11400808158553012No Hit
NAGGANNNNGNTATGANNAAACCCTCGACCCTGAGGCTGGAAGCGCCCCA2340.11256494131229557No Hit
NAGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC2330.1120838945545507No Hit
NNGGANNNNGNTATGANNCCACCCTCATGCCTAACCCCAGCACAGGGAGT2320.11160284779680583No Hit
NNGGANNNNGNTATGNNNACCTCTCTGGGCCTGTCTCCGTGTGCGCTGGA2310.111121801039061No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2310.111121801039061No Hit
NNGGANNNNGNTATGNNNCCCTGCGGCTCCTTTCAAGTCTAAGGGGACAG2290.1101597075235713No Hit
NNGGANNNNGNTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA2290.1101597075235713No Hit
NNGGANNNNGNTATGNNNCTTGGTGGAGCCATAGGCCGTCGTTTTACATG2280.10967866076582644No Hit
NAGGANNNNGNTATGNNNGCTGCTGACCCAACCCTACCTCTGCAGCTAGC2270.10919761400808158No Hit
NNGGANNNNGNTATGANNGCATCCTGCTTAGTGCTGCCCACAGTGTCATT2260.10871656725033674No Hit
NNGGANNNNGNTATGNNNTTTTTGTGCCCTTCTGCCCATGCTGGGTGGGA2250.10823552049259189No Hit
NNGGANNNNGNTATGANNCACGACACCCCACCTCTTGGACACTGCCCACC2250.10823552049259189No Hit
NNGGANNNNGNTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA2240.10775447373484702No Hit
NAGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT2230.10727342697710218No Hit
NNGGANNNNGNTATGNNNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC2220.10679238021935732No Hit
NNGGANNNNGNTATGNNNTGGGGATGAGGACAGCAGGGCCCCGGGAGCTG2190.10534923994612276No Hit
NNGGANNNNGNTATGNNNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC2180.1048681931883779No Hit
NNGGANNNNGNTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG2150.10342505291514337No Hit
NAGGANNNNGNTATGNNNGCTAATGCTTACTCTTTGCGTGAGTACTGGTT2140.1029440061573985No Hit
NNGGANNNNGNTATGNNNGGCGGGCCCTGATATAGAGCCACTGGCCGTCG2100.10101981912641908No Hit
NAGGANNNNGNTATGANNCAGGGTGACTCCGATCCTGGAGCCAACGGTGC2100.10101981912641908No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)