FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63253
Sequences flagged as poor quality0
Sequence length300
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTCAAGCAGACAGAGAGCTGAAGGAGTGCCAAGG10521.6631622215547088No Hit
TGTAAAACGACGGCCAGTAAGAGGTGAGCGAGGTCTGCGCCACGTGATCC9881.5619812499011905No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC9781.5461717230803282No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA9501.5019050479819138No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGGTTGGAGGTG9471.497162189935655No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTGGGGTTAGGCAT8491.3422288270912053No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCTCCTTCCCAT8221.299543104674877No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGCCCCAATCCTGTCTC7651.2094288017959622No Hit
TGTAAAACGACGGCCAGTCCCTGTCCTGAGCACCGTTGGCTCCAGGATCG7131.1272192623274784No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAGGACGGGTGGCC6821.0782097291828057No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGCCTGGGTGGGA6591.0418478174948225No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCCAAGAGGTGGGG6361.005485905806839No Hit
TGTAAAACGACGGCCAGTTGTCTGGTGGTGGAAGGAGGTGCACGCCACCA6270.9912573316680632No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG6170.975447804847201No Hit
TGTAAAACGACGGCCAGTGGCTCCCCAGCTGTCTCTCTTGTAAAACGACG5990.9469906565696488No Hit
TGTAAAACGACGGCCAGTGCCAATCCTGAGGCCATCAACACAAGGACTGG5800.9169525556100107No Hit
TGTAAAACGACGGCCAGTAGGTCATCCTCCTGCCTCGGGTCAGTGTTCCT5560.8790096912399412No Hit
TGTAAAACGACGGCCAGTGGTGTTACCGTGGTCACCCGCAGATTTGGGGC5510.8711049278295101No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC5250.8300001580952682No Hit
TGTAAAACGACGGCCAGTAGTCAGCCACGTTGGAACCAGTACTCACGCAA5110.8078668205460611No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGC5010.7920572937251988No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGTTCGGAGGCAGG4830.7636001454476468No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGACAGGGTCAGT4810.7604382400834743No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCATAAACACAAA4360.6892953693895942No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGCCTTCGGAGGC4350.687714416707508No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCAGCAGGATGGAG4180.660838221112042No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATCACATTTCAGCA4080.6450286942911798No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA3880.6134096406494554No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCGTGACCGAGAGCT3740.5912763031002483No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGAAGCCCAGTCAGG3540.5596572494585237No Hit
TGTAAAACGACGGCCAGTTCCCACCACCATACCCCAACTTGTAAAACGAC3540.5596572494585237No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCAGGTGACTCCAG3470.5485905806839202No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC3430.5422667699555752No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAGGCACGCAGGCT3420.5406858172734891No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGACATTCCTGCAA3390.5359429592272303No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCAGGGCTTGAACCC3250.5138096216780231No Hit
TGTAAAACGACGGCCAGTAGTGGAAGCTATTAGCACCAATGACAAGCAAC3140.4964191421750747No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGTGTCGTGGGGCA3080.48693342608255735No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGCCAGTTAACCA3080.48693342608255735No Hit
TGTAAAACGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT3010.4758667573079538No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACACGGCAGGAGA2990.4727048519437813No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCACATGCCTCCC2990.4727048519437813No Hit
TGTAAAACGACGGCCAGTCAAGCAGACAGAGAGCTGAAGGAGGAGGACCC2910.46005723048709146No Hit
TGTAAAACGACGGCCAGTTCTTTGGAATTTAGTGTCTGAGCCTTGGATAC2840.44899056171248797No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCAGGAAATCAGGCT2760.4363429402557981No Hit
TGTAAAACGACGGCCAGTTGCTGCATATTATTCCATTATGCAGACAGGAC2750.434761987573712No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGTCCAACAATGGCCTG2710.428438176845367No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA2670.42211436611702213No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT2650.41895246075284964No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCAAGAGGTGGGGT2650.41895246075284964No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATCACGGTGGT2640.41737150807076345No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGCGTGGGGCACTC2500.39523817052155624No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC2490.39365721783947No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG2480.39207626515738386No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGCTGAGGGAGGAT2450.3873334071111252No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT2440.38575245442903894No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCAGAAATCAGGCTG2420.3825905490648665No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAGGATCCCCGGTG2390.3778476910186078No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGTGCTGCTGAATG2390.3778476910186078No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCAAGGAATTCAG2280.36045721151565935No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCACCACGTGCAGGA2250.35571435346940067No Hit
TGTAAAACGACGGCCAGTAGTGGAAGCTATTAGCACCAATGACAGCCTCC2190.3462286373768833No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCCGAGTAGCTGAG2140.3383238739664522No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTATGGGCTCACT2130.33674292128436595No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGGGGAGGATCCTC2120.3351619686022797No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGAGGCTCCTTCC2110.3335810159201935No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCATAAACGCAAG2030.3209333944635037No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGACCTGGCGCAGGC1990.3146095837351588No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGAGTCCCCACACAC1970.31144767837098636No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAAGCCCCAGACCC1950.3082857730068139No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGCGCAGCCCTCCTAC1920.30354291496055524No Hit
TGTAAAACGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT1900.3003810095963828No Hit
TGTAAAACGACGGCCAGTCCAGAGATCAATAATAGCTCAGGGGAGATGGA1850.29247624618595164No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT1850.29247624618595164No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAGGAGAGTGA1770.27982862472926184No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGTCATAGCTGTTT1720.27192386131883073No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTCTCGGCTCACT1720.27192386131883073No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGTGAACGAAG1710.27034290863674454No Hit
TGTAAAACGACGGCCAGTGCCTGTGCCTGACCACTACACACTGGTCATAG1670.2640190979083996No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT1670.2640190979083996No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTACCCAGTGGTGTG1670.2640190979083996No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTCTCCACTCACT1670.2640190979083996No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCTCCCCAGTCACC1650.2608571925442271No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTAGCCTCTCCATT1640.25927623986214093No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACACTGCCGAGTG1590.25137147645170976No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT1580.24979052376962357No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCAGTTCACT1580.24979052376962357No Hit
TGTAAAACGACGGCCAGTTGCCCATCAGCAATCCTGCCACAAGGACACAG1570.24820957108753736No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTCCCGACGCCAGG1530.24188576035919246No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCCCCAGTAGCTGG1520.2403048076771062No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCTGCAGACAGGAT1520.2403048076771062No Hit
TGTAAAACGACGGCCAGTAGCCTTTCTACTCATTGGAACACTCAGGTGTA1510.23872385499501997No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTGCCAGTACCGGG1450.22923813890250266No Hit
TGTAAAACGACGGCCAGTTGCCCATCAGCAATCCTGCCTTCGACATACAA1400.22133337549207155No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGTGTAAAACGACG1380.21817147012789906No Hit
TGTAAAACGACGGCCAGTTCCCACCACCATACCCCAACTCAGGCGGGAGG1370.21659051744581287No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGTGTTCGGAGGCA1360.21500956476372662No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCCAAAGTGCTGGG1360.21500956476372662No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTCTTGGCTCACT1340.2118476593995542No Hit
TGTAAAACGACGGCCAGTGAACAGCCCCGGAAGCAAGTTTTACAAGAGGG1330.21026670671746794No Hit
TGTAAAACGACGGCCAGTCAAGCAGACAGAGAGCTGAAGGAATTAGTAGT1310.2071048013532955No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCACCCCTGCCAA1310.2071048013532955No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTGATAAGGAAGGA1280.2023619433070368No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTGACCCTTCCAGGA1260.19920003794286437No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCACTAGGAGAG1250.19761908526077812No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA1230.1944571798966057No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG1190.1881333691682608No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCAGCCCCTGCCA1160.1833905111220021No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATCGCATTTCAGCA1120.17706670039365724No Hit
TGTAAAACGACGGCCAGTCCATCCCACAGCCATCCGTGCCAGCCCCCCAC1100.17390479502948478No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCAGCTCACT1050.16600003161905363No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGTGTAAAACTACG1040.16441907893696742No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCATAAACGCAAA1030.16283812625488117No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTCTAGCCTGGGCA1020.16125717357279495No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCAGGGAACAACT1020.16125717357279495No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGATTGAAAATCTGC1020.16125717357279495No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGACACTGTGAGGCCC1020.16125717357279495No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCACCCCCTGCCA1010.15967622089070874No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGATAGGGCCAGGC1010.15967622089070874No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCACTTCCCCTGCAC1010.15967622089070874No Hit
TGTAAAACGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA1000.15809526820862252No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGGTGTCCTGCGTG990.15651431552653627No Hit
TGTAAAACGACGGCCAGTCCTTGAAGGCTGGGCTTCTGTCTTCTGGCAGG990.15651431552653627No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGAGGCTTTTTGTTCCA970.15335241016236384No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAGTAGGAGAG970.15335241016236384No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCAGTTTCCTGCC960.15177145748027762No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGGTCTGGGTG960.15177145748027762No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGCTTGCAGCACA940.14860955211610516No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCCAGGATGGAGGG920.14544764675193272No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTGACTCAAATGTT880.13912383602358783No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCTGGCCCAAGTCA880.13912383602358783No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCCCCCCAGGATGG860.13596193065941536No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACACCGCCGAGTG860.13596193065941536No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGGGAGGATCCTCC850.13438097797732915No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCTACTTCAATGC850.13438097797732915No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCATACCCCGGCC820.12963811993107047No Hit
TGTAAAACGACGGCCAGTTGGCCCACTGTGCTAGCCTAGGAACCATGGCT820.12963811993107047No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCATAATCCCCCA800.126476214566898No Hit
TGTAAAACGACGGCCAGTCCTTGAAGGCTGGGCTTCTGTGGGTGGGGAAG780.12331430920272556No Hit
TGTAAAACGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT770.12173335652063934No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCACCTTGGCCTCC770.12173335652063934No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCTGAGAAGGACCT760.1201524038385531No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCACAGCACTGCC740.11699049847438067No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCGGTCATAGCCTCT740.11699049847438067No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTGGAAGGGGTTAG730.11540954579229443No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGCCATCTCTGCTC720.11382859311020821No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGGAGGATCCTCCC710.11224764042812199No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA710.11224764042812199No Hit
TGTAAAACGACGGCCAGTCCCTGTCCTGAGCACCGTTGCTAACCCCCAGT710.11224764042812199No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTCTGGGCTCACT700.11066668774603577No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGGCAGGAGAG700.11066668774603577No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTGGAGGCCAGGAG700.11066668774603577No Hit
TGTAAAACGACGGCCAGTTGGCCCACTGTGCTAGCCTACAGGAACCATGG690.10908573506394953No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCACCAGCACGAC690.10908573506394953No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCCCCGGACACCA680.10750478238186331No Hit
TGTAAAACGACGGCCAGTTGTCTGGTGGTGGAAGGAGGGGGTGGCTGCGC680.10750478238186331No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCGGTCATAGCTAGC680.10750478238186331No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCTGGAGGGCGGCCA640.1011809716535184No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)