FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003246398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54595
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNATCCACTGTTGGCCAACACACCTGGCCTTCAA2650.48539243520468905No Hit
NNGGANNNNGNTATGNNNTGGAAACCCATGCCATGCACTCAACACTACAA2120.38831394816375125No Hit
NNGGANNNNGNTATGNNNCCTGGACGCAATCACCCAGGCTGCAGTGCGGT1860.3406905394266874No Hit
NNGGANNNNGNTATGANNATCCACTGTTGGCCAACACACCTGGCCTTCAA1860.3406905394266874No Hit
NNGGANNNNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA1770.3242055133253961No Hit
NNGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1690.3095521567909149No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC1690.3095521567909149No Hit
NAGGANNNNGNTATGNNNATCCACTGTTGGCCAACACACCTGGCCTTCAA1440.2637604176206612No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1420.26009707848704094No Hit
NAGGANNNNGNTATGNNNTGGAAACCCATGCCATGCACTCAACACTACAA1350.2472753915193699No Hit
NNGGANNNNGNTATGANNCCTGGACGCAATCACCCAGGCTGCAGTGCGGT1280.2344537045516989No Hit
NNGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC1240.2271270262844583No Hit
NNGGANNNNGNTATGNNNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1240.2271270262844583No Hit
NNGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1210.22163201758402784No Hit
NNGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA1190.21796867845040754No Hit
NAGGANNNNGNTATGANNATCCACTGTTGGCCAACACACCTGGCCTTCAA1190.21796867845040754No Hit
NNGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGGACACTGCCCAC1170.21430533931678727No Hit
NNGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1150.21064200018316698No Hit
NAGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC1140.20881033061635682No Hit
NAGGANNNNGNTATGNNNCCTGGACGCAATCACCCAGGCTGCAGTGCGGT1130.20697866104954668No Hit
NAGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1080.19782031321549592No Hit
NNGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGAGGGGTATAT1070.19598864364868576No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCAGGAAACAGCT1050.1923253045150655No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1040.19049363494825533No Hit
NNGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA1040.19049363494825533No Hit
NNGGANNNNGNTATGNNNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC1000.18316695668101474No Hit
NNGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC990.1813352871142046No Hit
NNGGANNNNGNTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT950.174008608846964No Hit
NNGGANNNNGNTATGNNNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC900.16485026101291328No Hit
NNGGANNNNGNTATGNNNAGAGGCACAGGTGGAGAGGGACGAGACCCTGA880.16118692187929295No Hit
NNGGANNNNGNTATGNNNCTTGAGCTCCTCGGACACGGCAGGAAACAGCT870.15935525231248282No Hit
NNGGANNNNGNTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA870.15935525231248282No Hit
NNGGANNNNGNTATGNNNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC860.15752358274567269No Hit
NNGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG820.1501969044784321No Hit
NNGGANNNNGNTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA820.1501969044784321No Hit
NNGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA820.1501969044784321No Hit
NNGGANNNNGNTATGNNNTGCCAAAATTAAGGATGCACACCTGGAGTCAC810.14836523491162196No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC800.1465335653448118No Hit
NNGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCTGCCCAACACCC800.1465335653448118No Hit
NAGGANNNNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA790.14470189577800166No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACCCTTTGTCCA790.14470189577800166No Hit
NNGGANNNNGNTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC790.14470189577800166No Hit
NNGGAAANNGNTATGANNATCCACTGTTGGCCAACACACCTGGCCTTCAA790.14470189577800166No Hit
NAGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT780.1428702262111915No Hit
NNGGANNNNGNTATGNNNTAGAGGACACCCAAGGCCACCCGTCCTCGGAC780.1428702262111915No Hit
NNGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCAGGAAACAGCT770.14103855664438136No Hit
NNGGANNNNGNTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC770.14103855664438136No Hit
NAGGAAANAGCTATGANNATCCACTGTTGGCCAACACACCTGGCCTTCAA770.14103855664438136No Hit
CAGGAAANAGCTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA760.13920688707757123No Hit
NNGGANNNNGNTATGNNNCCCGTGACAGCTCCTGCAATTACAAAATGGCT750.13737521751076104No Hit
NNGGANNNNGNTATGNNNGGGTCCCACCCATGCTGAAATGTGATCCTCAA750.13737521751076104No Hit
NNGGANNNNGNTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC740.1355435479439509No Hit
NNGGANNNNGNTATGNNNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT730.13371187837714077No Hit
NAGGANNNNGNTATGNNNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA720.1318802088103306No Hit
NAGGAANNNGNTATGANNATCCACTGTTGGCCAACACACCTGGCCTTCAA720.1318802088103306No Hit
NNGGANNNNGNTATGNNNCAGGGTGACTCCGATCCTGGAGCCAACGGTGC700.1282168696767103No Hit
NNGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT700.1282168696767103No Hit
CAGGAAANAGCTATGANNATCCACTGTTGGCCAACACACCTGGCCTTCAA700.1282168696767103No Hit
NAGGAAANAGNTATGANNATCCACTGTTGGCCAACACACCTGGCCTTCAA680.12455353054309003No Hit
NNGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG680.12455353054309003No Hit
NNGGANNNNGNTATGANNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT680.12455353054309003No Hit
NNGGANNNNGNTATGANNGCTAATGCTTACTCTTTGCGTGAGTACTGGTT680.12455353054309003No Hit
NNGGANNNNGNTATGNNNGCTAATGCTTACTCTTTGCGTGAGTACTGGTT680.12455353054309003No Hit
NNGGANNNNGNTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC680.12455353054309003No Hit
NNGGANNNNGNTATGNNNCAGGGCTCCAAGAATGTTCCACACATCTTTCT680.12455353054309003No Hit
NNGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC670.12272186097627988No Hit
NAGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA660.12089019140946973No Hit
NAGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT660.12089019140946973No Hit
NNGGANNNNGNTATGNNNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT650.1190585218426596No Hit
NAGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC640.11722685227584945No Hit
NAGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGAGGGGTATAT640.11722685227584945No Hit
NNGGAAANNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA640.11722685227584945No Hit
NNGGANNNNGNTATGANNACCTTTACCCAAATAGCTACCACCAGTGCCTT630.1153951827090393No Hit
NNGGANNNNGNTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA630.1153951827090393No Hit
NNGGANNNNGNTATGNNNACCTGGCAGCCCCAAATCTGCGGGTGACCACG630.1153951827090393No Hit
NNGGANNNNGNTATGNNNCTCGTGCAGTCTGGTGTCCGCAGGAAACAGCT630.1153951827090393No Hit
NAGGANNNNGNTATGANNCCTGGACGCAATCACCCAGGCTGCAGTGCGGT630.1153951827090393No Hit
NNGGANNNNGNTATGNNNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG620.11356351314222915No Hit
NNGGANNNNGNTATGNNNGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA620.11356351314222915No Hit
NNGGANNNNGNTATGANNCTTGAGCTCCTCGGACACGGCAGGAAACAGCT620.11356351314222915No Hit
NAGGAANNNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA610.11173184357541899No Hit
NAGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGGACACTGCCCAC610.11173184357541899No Hit
NNGGANNNNGNTATGNNNACCTTTACCCAAATAGCTACCACCAGTGCCTT610.11173184357541899No Hit
NAGGANNNNGNTATGNNNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC610.11173184357541899No Hit
NNGGANNNNGNTATGANNCTCGTGCAGTCTGGTGTCCGCAGGAAACAGCT610.11173184357541899No Hit
NNGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGACACTGCCCACC600.10990017400860884No Hit
NAGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA600.10990017400860884No Hit
NAGGANNNNGNTATGNNNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT590.10806850444179869No Hit
NNGGANNNNGNTATGNNNACCTCTCTGGGCCTGTCTCCTCCCCGCCAAGA590.10806850444179869No Hit
NAGGAAANAGCTATGANNCCTGGACGCAATCACCCAGGCTGCAGTGCGGT580.10623683487498854No Hit
NNGGANNNNGNTATGANNGGGTCCCACCCATGCTGAAATGTGATCCTCAA580.10623683487498854No Hit
NAGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC580.10623683487498854No Hit
NAGGAAANAGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA580.10623683487498854No Hit
NNGGANNNNGNTATGANNAGAGGCACAGGTGGAGAGGGACGAGACCCTGA570.10440516530817841No Hit
NAGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCAGGAAACAGCT570.10440516530817841No Hit
NAGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT570.10440516530817841No Hit
NNGGANNNNGNTATGNNNGGGGAGACTGGGCCATACGATAGGCTTAATCC560.10257349574136826No Hit
NAGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC560.10257349574136826No Hit
NAGGAANNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT550.10074182617455811No Hit
NAGGANNNNGNTATGNNNACCTTTACCCAAATAGCTACCACCAGTGCCTT550.10074182617455811No Hit
CAGGAAANAGCTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC550.10074182617455811No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)