FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003246399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227278
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTTGAGAGGGACTGGAGAAAGGGAACTTACAGAG98674.341379280000704No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG90703.9907074155879583No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA85093.7438731421431024No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT80323.5339980112461387No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG77233.3980411654449614No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG72663.1969658303927346No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGTGAACGAAG71023.1248075044658963No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA58652.58054013146895No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA45752.012953299483452No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC43261.9033958412164838No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC39141.722120046814914No Hit
TGTAAAACGACGGCCAGTCTCTCACCCACCTCCACCCATCAAAGGTGCCT38981.715080210139125No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT32971.450646345004796No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC32061.4106072739112452No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG31451.3837678965847993No Hit
TGTAAAACGACGGCCAGTGGGGTGGGGAGTGAGATGGTCCTAGGAAGTAC29521.298849866683093No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCTGGAGGGCGGCCA29301.289170091253883No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCTGGAGGGCAGCCA27741.220531683664939No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC25571.125053898749549No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC24491.0775350011879725No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA23891.0511356136537633No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG21940.9653376041675833No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC21750.9569777981150838No Hit
TGTAAAACGACGGCCAGTAGCAGGCAGGTCCTTCCAGACAGCCAATGAGG21460.9442180941402158No Hit
TGTAAAACGACGGCCAGTTGGATGGTGAGGGAAGGGCTAAGACAGGATTC20350.895379227201929No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG17800.7831818301815399No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC17170.7554624732706201No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG17040.7497426059715415No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG15880.6987037900720704No Hit
TGTAAAACGACGGCCAGTAGGAGGGCGGAGGAGGAAAGCAGGTTTGCTCT15560.6846241167204922No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGTCCCTGTTTTACAATGCA14550.6401851477045732No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC13850.6093858622479958No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCATTGTGGGCTA12870.5662668626087876No Hit
TGTAAAACGACGGCCAGTCCTCACTCCCCACCCAGGATCTGGGCACTGGA12650.5565870871795774No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA12250.5389874954901046No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTTGGACCCAGAG11880.5227078731773422No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC11690.5143480671248427No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA11450.503788312111159No Hit
TGTAAAACGACGGCCAGTCTGTCTGGCATGGGCCTCTCCACCGTGCCTGA11410.5020283529422118No Hit
TGTAAAACGACGGCCAGTAGGTCATCCTCCTGCCTCGGCCTCCCAAAGTG11360.4998284039810276No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA10710.47122906748563437No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC10680.4699090981089239No Hit
TGTAAAACGACGGCCAGTTCAGGGAGGCGGGGCTCGTGCCACAGGGACAC9390.4131504149103741No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG9240.4065505680268218No Hit
TGTAAAACGACGGCCAGTTCAGGGAGGCGGGGCTCGTTCCACAGGGACAC8900.39159091509076993No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT8450.371791374440113No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCACTGCCTAGCAGG5100.22439479404077825No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGCTACGGGGT5000.21999489611841003No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT4690.20635521255906863No Hit
TGTAAAACGACGGCCAGTCTGGGCCTTGGCTTCTTCCTCAATCATCTAGG4330.1905155800385431No Hit
TGTAAAACGACGGCCAGTTCCATTACTCTACACAAGCAGGGTACACTTAG4090.1799558250248594No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCGCCTCGGCCTCC3640.1601562843742025No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGGCTACGGGGTCA3520.15487640686736068No Hit
TGTAAAACGACGGCCAGTGCCATCGGCTCCCAAGTTTTATGTCCTAGAGC3460.15223646811393976No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGATATCTCCTGCAC3320.14607661102262426No Hit
TGTAAAACGACGGCCAGTGCCAATCCTGAGGCCATCAAAGTGACAGGTAG3130.1377168049701247No Hit
TGTAAAACGACGGCTAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG2890.127157049956441No Hit
TGTAAAACGACGGCTAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG2850.12539709078749373No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGACCGAGTAGCCACAG2840.1249571009952569No Hit
TGTAAAACGACGGCTAGTTGAGAGGGACTGGAGAAAGGGAACTTACAGAG2700.11879724390394142No Hit
TGTAAAACGACGGCTAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT2670.11747727452723097No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGACCGGGTAGCCACAG2610.11483733577381004No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGAGCTCTGAATTGTCT2610.11483733577381004No Hit
TGTAAAACGACGGCCAGTGACTGTAGGTTGGGCTCCCCATGTGCTGTGCC2600.11439734598157324No Hit
TGNAAAACGACGGCCAGTTGAGAGGGACTGGAGAAAGGGAACTTACAGAG2590.11395735618933642No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC2580.1135173663970996No Hit
TGTAAAACGACGGCCAGTTCTTCACCTCCCAAAATGCTGGGATTACAGGC2490.10955745826696821No Hit
TGTAAAACGACGGCCAGTACCTCATGTCACGTGCGTCCATGTGAAGAGAG2470.10867747868249457No Hit
TGTAAAACGACGGCCAGTTGAGAGGGACTGGAGAACGGGAACTTACAGAG2450.10779749909802093No Hit
TGTAAAACGACGGCTAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA2440.1073575093057841No Hit
TGNAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCTGGGTAGCCACAG2420.10647752972131046No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCGGGTGCCAGTGG2380.10471757055236319No Hit
TGTAAAACGACGGCTAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG2340.1029576113834159No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)