FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003246400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227278
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT75583.3254428497258863No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG46312.037592727848714No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG39561.7405996180888603No Hit
CAGGAAANAGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT36741.6165224966780771No Hit
CAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT35341.5549239257649223No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA33561.4766057427467683No Hit
CAGGAAANAGCTATGANNCCGTAGCCACGCCCCTATTGTCTGACCACGCT31561.3886077842994042No Hit
CAGGAAANAGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT21860.9618176858296887No Hit
CAGGAAANAGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG20530.9032990434621916No Hit
CAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG17280.7603023609852252No Hit
CAGGAAANAGCTATGANNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT16870.7422627795035155No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC14150.6225855560151005No Hit
CAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA14110.6208255968461531No Hit
CAGGAAANNGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG14110.6208255968461531No Hit
CAGGAAANAGCTATGANNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT13370.5882663522206285No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT13050.5741866788690503No Hit
CAGGAAANAGCTATGANNAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG13040.5737466890768134No Hit
CAGGAAANNGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT12930.5689068013622084No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT12200.5367875465289205No Hit
CAGGAAANNGCTATGANNCCGTAGCCACGCCCCTATTGTCTGACCACGCT11780.5183079752549741No Hit
CAGGAAANNGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA11700.5147880569170795No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG11240.4945485264741858No Hit
CAGGAAANAGCTATGANNAGTGGGAGGAGCTCTCGGTCCCCTCTTTTAGG11090.48794867959063354No Hit
CAGGAAANAGCTATGANNGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG10850.4773889245769498No Hit
CAGGAAANAGCTATGANNGCTGCTGACCCAACCCTACCCTGTCCAGCCCC10160.4470296289126092No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT9780.43031001680761005No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA9760.4294300372231364No Hit
NAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT9750.4289900474308996No Hit
CAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT8710.38323110903827035No Hit
NAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT8640.3801511804926126No Hit
CAGGAAANNGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT8330.3665114969332711No Hit
CAGGAAANAGCTATGANNTGGGTTGCCTGGGACTGAGAGGGTCCTGGGAC8300.36519152755656065No Hit
CAGGAAANAGCTATGANNGCCCTGGCCACTGTGGTTAATGAAACTTGGTT7940.3493518950360352No Hit
CAGGAAANAGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG7900.34759193586708786No Hit
CAGGAAANAGCTATGANNAAGTTGGTCAGCCCTGGTGACTCATTGGATAT7670.337472170645641No Hit
CAGGAAANAGCTATGANNAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT7070.3110727831114318No Hit
CAGGAAANAGCTATGANNTCCAGTGGTGGGGCTCAACAAAGGCTCTGTGA7020.3088728341502477No Hit
CAGGAAANAGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT6900.30359295664340585No Hit
CAGGAAANAGCTATGANNTCCAGTGGTGGGGCTCAACAAAGGCTCCGTGA6880.30271297705893224No Hit
CAGGAAANAGCTATGANNTGGGCCTGGACACTTTGACGGCGGGGCACGTC6780.298313079136564No Hit
CAGGAAANNGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG6600.2903932628763013No Hit
CAGGAAANAGCTATGANNCGGGACCCAAAGTTCTGCGTGATGGTCAGCAC6560.288633303707354No Hit
CAGGAAANAGCTATGANNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG6260.2754336099402494No Hit
CAGGAAACAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT5930.2609139467964343No Hit
CAGGAANNNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT5780.254314099912882No Hit
CAGGAAANAGCTATGANNTGTTGTCTTTCAGGGGCCGCCATGCCTGCCGT5680.2499142019905138No Hit
CAGGAAANAGCTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC5520.2428743653147247No Hit
CAGGAAACNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT5500.24199438573025106No Hit
CAGGAAANNGCTATGANNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT5320.2340745694699883No Hit
NAGGANANNGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG5320.2340745694699883No Hit
CAGGAAANNGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA5240.23055465113209372No Hit
CAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC5150.22659474300196236No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA5150.22659474300196236No Hit
CAGGAAANAGCTATGANNTCCCTTAAACTTGGGATTGCTCCATTGCACTC5110.22483478383301508No Hit
CAGGAAANAGCTATGANNTGACAGCACAAGGAACACTGACCTACAGATAA5090.22395480424854144No Hit
CAGGAAANNGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT5040.22175485528735736No Hit
CAGGAAANAGCTATGANNGCCCCTACTCCAAAAGGCGGATGAATGGGGAA4970.2186749267416996No Hit
CAGGAAANNGCTATGANNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT4920.2164749777805155No Hit
CAGGAAANNGCTATGANNAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG4920.2164749777805155No Hit
CAGGAAANAGCTATGANNGCTACGGGCAATCACTCTCCTACTCCCAACCA4870.2142750288193314No Hit
CAGGAAANAGCTATGANNGGTAGCAACGTCCCACCCAGCTGAATAGTTGT4860.21383503902709455No Hit
NAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG4760.20943514110472639No Hit
CAGGANANNGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG4620.2032752840134109No Hit
CAGGAAANNGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA4610.20283529422117408No Hit
CAGGAAANAGCTATGANNTGGGTTATGGCGTGGGATGCCCTGCAACCCCC4550.20019535546775316No Hit
NAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT4540.19975536567551633No Hit
CAGGAAANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG4500.19799540650656905No Hit
CAGGANANNGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG4320.1900755902463063No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT4270.18787564128512219No Hit
NAGGAAANNGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT4240.18655567190841174No Hit
CAGGAAANNGCTATGANNGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG4090.1799558250248594No Hit
CAGGAAANAGCTATGANNGCTACGGGCAATCACTCTCCTATTCCCAACCA4050.17819586585591216No Hit
CAGGAAANNGCTATGANNAGTGGGAGGAGCTCTCGGTCCCCTCTTTTAGG3990.1755559271024912No Hit
NAGGANANNGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT3970.1746759475180176No Hit
NAGGANANNGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG3960.17423595772578077No Hit
CAGGAAANNGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT3870.17027604959564938No Hit
CAGGANANNGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT3840.16895608021893893No Hit
NAGGAAANAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3750.16499617208880754No Hit
CAGGAAANNGCTATGANNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT3680.16191624354314982No Hit
NAGGAAANNGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA3670.16147625375091296No Hit
NAGGAAANNGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG3620.15927630478972887No Hit
CAGGAAANAGCTATGANNTGCTGCCGAGAGAGGACAGTGATCCCCCAAAA3540.15575638645183432No Hit
NAGGANANNGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA3470.15267645790617657No Hit
NAGGANANNGCTATGANNCCGTAGCCACGCCCCTATTGTCTGACCACGCT3370.14827655998380837No Hit
NAGGAAANNGCTATGANNCCGTAGCCACGCCCCTATTGTCTGACCACGCT3330.1465166008148611No Hit
CAGGAAANNGCTATGANNGCTGCTGACCCAACCCTACCCTGTCCAGCCCC3320.14607661102262426No Hit
CAGGANANNGCTATGANNCCGTAGCCACGCCCCTATTGTCTGACCACGCT3220.1416767131002561No Hit
CAGGANANNGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA3190.14035674372354562No Hit
CAGGAAACAGCNATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3050.13419688663223012No Hit
CAGGAAANAGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT3010.13243692746328287No Hit
CAGGAAANNGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG2950.12979698870986192No Hit
CAGGAAANNGCTATGANNGCCCTGGCCACTGTGGTTAATGAAACTTGGTT2950.12979698870986192No Hit
CAGGAANNNGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG2830.12451711120302009No Hit
CAGGAAANNGCTATGANNTCCAGTGGTGGGGCTCAACAAAGGCTCCGTGA2800.12319714182630964No Hit
CAGGAAANAGCTATGANNCCGTAGCCACGCCCCTATTGCAGGAAACAGCT2790.12275715203407282No Hit
CAGGAAANNGCTATGANNTGGGTTGCCTGGGACTGAGAGGGTCCTGGGAC2660.11703728473499415No Hit
CAGGAAANNGCTATGANNAAGTTGGTCAGCCCTGGTGACTCATTGGATAT2600.11439734598157324No Hit
NAGGAAANAGCTATGANCTGTTTTGCCCCTGAAATGAATATCTAAACCAG2580.1135173663970996No Hit
CAGGAAACNGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG2520.11087742764367868No Hit
CAGGAAANNGCTATGANNTCCAGTGGTGGGGCTCAACAAAGGCTCTGTGA2510.11043743785144183No Hit
CAGGAAACAGCNATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG2510.11043743785144183No Hit
CAGGAAANAGCTATGANNTCCCACTTGCTCTTTGAAGGCAATTCCATTCT2490.10955745826696821No Hit
CAGGAAANNGCTATGANNACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA2480.10911746847473139No Hit
CAGGAAANNGCTATGANNTGGGCCTGGACACTTTGACGGCGGGGCACGTC2460.10823748889025775No Hit
NAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG2450.10779749909802093No Hit
NAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG2430.10691751951354728No Hit
CAGGAAACAGCTATGANCTGTTTTGCCCCTGAAATGAATATCTAAACCAG2420.10647752972131046No Hit
CAGGAAACAGCNATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG2410.10603753992907364No Hit
CAGGAAANNGCTATGANNAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT2400.10559755013683683No Hit
NAGGAAANAGCTATGANCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT2370.10427758076012636No Hit
CAGGAAANNGCTATGANNCGGGACCCAAAGTTCTGCGTGATGGTCAGCAC2360.10383759096788954No Hit
CAGGAAACNGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT2280.10031767262999498No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)