FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94704
Sequences flagged as poor quality0
Sequence length300
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCAGGTTGCTGGATTCAGTTTGCTGACCAACTTT22142.3378104409528637No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA18131.9143858759925663No Hit
CAGGAAACAGCTATGACCGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC17921.8922115222165907No Hit
CAGGAAACAGCTATGACCTTGGACAGCACCTTGAATGCCTGGGTTCTAAC17531.8510305794897788No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT16271.7179844568339246No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGCACTCCTTC15621.6493495522892379No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCCGTCCTCGGAC15171.6018330799121474No Hit
CAGGAAACAGCTATGACCTGACTGAAAGGCTTTTCCACGTGCACTGCTGG14391.5194711944585235No Hit
CAGGAAACAGCTATGACCTCCTGCCCCACCCTGATACAGGTCACTGTGGA12801.3515796587261362No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCACCCCACCCTGCT12731.3441882074674776No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCTGGGTGCTGGTG12121.2797769893563102No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTCCCCAACCATAG11991.266050008447373No Hit
CAGGAAACAGCTATGACCTGACAGCACAAGGAACACTGACCCGAGGCAGG11921.2586585571887143No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC11891.255490792363575No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACCCTGTCTCTCTAGC11851.2512671059300557No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC10631.1224446697077208No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCTGCCCAACACCC10111.0675367460719716No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC10011.0569775299881738No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA9090.9598327420172326No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGGCTGTTT8890.9387143098496368No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATGGTGAGCCCTGC8830.9323787801993579No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA8770.9260432505490792No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT8190.8647997972630512No Hit
CAGGAAACAGCTATGACCTCCAGCCAGCCAGTCCTTGTGTTGATGGCCTC7890.8331221490116574No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG7720.8151714816692008No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCAGGAAACAGCT7250.7655431660753506No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACGGAGATCCAGCC7250.7655431660753506No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGGAGTCAC6670.7042997127893225No Hit
CAGGAAACAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT6650.702187869572563No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCAAGCCTGACTG6480.6842372022301064No Hit
CAGGAAACAGCTATGACCGCAGCCACCCTGAACACACTGGGCAGGAAGAA6420.6779016725798277No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT6420.6779016725798277No Hit
CAGGAAACAGCTATGACCGGGTCCCACCCATGCTGAAATGTGATCCTCAA6120.6462240243284338No Hit
CAGGAAACAGCTATGACCGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA5620.5934279439094442No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGACACTGCAGCCTGGG5450.5754772765669877No Hit
CAGGAAACAGCTATGACCCCGTAGCCACGCCCCTATTGTCTGACCACGCT5350.5649180604831897No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG5110.5395759418820747No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT5080.5364081770569353No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCACGGGCATCCTG5010.5290167257982767No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTAGTCTC4890.5163456664977192No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAAGGCCGCAGCTCC4880.5152897448893394No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCTGGGTGTCTGC4840.5110660584558202No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGGTCCTCCTC4760.5026186855887819No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGCTCAAGG4570.4825561750295658No Hit
CAGGAAACAGCTATGACCGTGCTCCTTGGGGACGTAGTTTTACACAGAAC4440.46882919412062846No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTAGTTCC4390.46354958607872954No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG4240.44771076195303267No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAACGCAGCTCCCCA4200.44348707551951344No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAGGCCCCTGTACC3970.4192008785267782No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAACTGGCCAGCCTG3940.41603311370163876No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCGGGGATCCTCG3870.40864166244298017No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGTGCTGCCTCTCCA3850.4065298192262206No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC3820.4033620544010813No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACTGCCTCCCGACT3640.38435546545024496No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA3550.3748521709748268No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACCACCGTGATCTCTC3540.37379624936644706No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAGGGGACATGGCC3510.37062848454130765No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAACAGTGAAGACAG3480.3674607197161683No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA3450.36429295489102886No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGACACACCACT3450.36429295489102886No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAACAGCAGACACC3410.3600692684575097No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATGGAGCCCTGCAG3350.3537337388072309No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGGCTGCTT3320.3505659739820916No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC3300.348454130765332No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG3280.34634228754857244No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCCTCCCCACCTCC3140.3315593850312553No Hit
CAGGAAACAGCTATGACCGGCGGGCCCTGATATAGAGCCACTGGCCGTCG3130.33050346342287545No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCTCTCCACGTGC3070.32416793377259673No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTATTGCAGCCTGT3040.32100016894745736No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCAGATTTTCAAT2970.3136087176887988No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTGGGGAGGGTGG2960.312552796080419No Hit
CAGGAAACAGCTATGACCGGGTCCCACCCATGCTGAAATGCGATCCTCAA2830.29882581517148166No Hit
CAGGAAACAGCTATGACCGTGCTCCTTGGGGACGTAGTTGTTTCCTGCAG2810.2967139719547221No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG2740.28932252069606357No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAGGCACTACGT2730.2882665990876837No Hit
CAGGAAACAGCTATGACCGAGGCGCAGGTCACAGTGAGACCAATACAGAG2720.28721067747930396No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCTCTTTTAGG2710.28615475587092415No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTGTTCTGGAATCC2590.2734836965703666No Hit
CAGGAAACAGCTATGACCGGAAATAAGTACCTTCTGTGTCCTTGTATGTC2580.27242777496198683No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG2500.2639804020949485No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTTATCCC2490.2629244804865687No Hit
CAGGAAACAGCTATGACCGGCCTTCTCTGCATTATCTCGCTCAGGGAAAG2440.2576448724446697No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCAGGGCAGCGGT2320.24497381314411218No Hit
CAGGAAACAGCTATGACCAGACAGGGTCTTGCTATGTTGCCCAGGCTAGA2290.2418060483189728No Hit
CAGGAAACAGCTATGACCGTGCTCCTTGGGGACGTAGTCAGGGGCCACGC2290.2418060483189728No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT2250.23758236188545362No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGTCTGCAGCCTGG2230.23547051866869406No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACCTCCAACCCCGT2200.23230275384355467No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCATCCTGCACGTGG2160.2280790674100355No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTTGGGCCAGGGT2150.22702314580165567No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGAACAAAAAGCCTCAC2150.22702314580165567No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGTCTGGGTCCT2120.22385538097651633No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGATACTACTA2120.22385538097651633No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTATTGAGGCACTT2050.21646392971785777No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGGGGGCCTGGCT2040.21540800810947794No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTGATCAGGGTGG2030.21435208650109816No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTACGTGTCCCTAG1970.20801655685081938No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTGGCATGAAGGA1970.20801655685081938No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1840.1942895759418821No Hit
CAGGAAACAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT1830.19323365433350229No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATCCGAGTGAGGAG1790.18900996789998312No Hit
CAGGAAACAGCTATGACCGCTCCTGTGCTGTCAAGGGAACGAGGGCCAGT1780.1879540462916033No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC1770.18689812468322353No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACCATCCTGCCTCC1740.18373035985808414No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTTCCTGCAAAACA1720.18161851664132456No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTATGTGACT1710.18056259503294475No Hit
CAGGAAACAGCTATGACCGCAGCCACCCTGAACACACTCCACCAGGAAGA1690.17845075181618517No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCGCCCAACACCC1680.1773948302078054No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAACTGGCCGTCGTT1670.17633890859942558No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC1640.17317114377428622No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTATGACCGCCCACC1630.1721152221659064No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAACACGCAGCTCCC1620.1710593005575266No Hit
CAGGAAACAGCTATGACCTGACAGCACAAGGAACACTGACCGTCGTTTTC1600.16894745734076702No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGGTGATTCAGTAG1580.16683561412400744No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCAGAGGGAGCTAGG1570.16577969251562766No Hit
CAGGAAACAGCTATGACCTGACTGAAAGGCTTTTCCACGTGCACTACTGG1560.16472377090724785No Hit
CAGGAAACAGCTATGACCGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG1550.16366784929886807No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCAGGAAACAGCTA1540.16261192769048827No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGATGCTGCCTCCT1490.1573323196485893No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTAGTCCC1490.1573323196485893No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGCCTGGCTTGTC1480.1562763980402095No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACAGAGGAGCCGGT1460.15416455482344993No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGGTGACCCTAATC1440.15205271160669032No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGAAAAGTTTCAGG1430.15099678999831054No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGTCCTGTGCCCA1420.14994086838993073No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCCTGGGTGTCTG1390.14677310356479137No Hit
CAGGAAACAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG1390.14677310356479137No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCCAGGAAACAGC1380.14571718195641156No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTCACACCTGTAA1380.14571718195641156No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACGGCCCCTCTGCC1380.14571718195641156No Hit
CAGGAAACAGCTATGACCAGACAGGGTCTTGCTATGTTGCCCAGGCTGGT1360.14360533873965198No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT1350.14254941713127217No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTAGTGGCATGAA1320.1393816523061328No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTTGGCATGAAGG1250.13199020104747425No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCAGCTCCCCAGG1230.12987835783071466No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTGAGGAGGGTGG1210.12776651461395505No Hit
CAGGAAACAGCTATGACCGGCCTTCTCTGCATTATCTCGCTAATCTCGAG1110.11720729853015711No Hit
CAGGAAACAGCTATGACCAGACAGGGTCTTGCTATGTTGCCAAAGGAGAT1100.11615137692177734No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACCTGCCTCCCGAC1100.11615137692177734No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACTCTTGGCCTGCT1090.11509545531339753No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATCCAGCAGCAGAG1080.11403953370501775No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGTGGACTAGGTCC1070.11298361209663794No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTGTAATCCCAGC1040.10981584727149855No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTCCAGCACCTGTG1040.10981584727149855No Hit
CAGGAAACAGCTATGACCGGGTCCCACCCATGCTGAAAGTCCACCTTCCA1030.10875992566311878No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGGCTTTCA1030.10875992566311878No Hit
CAGGAAACAGCTATGACCTCCAGCCAGCCAGTCCTTGTCCTTATATCTTT1010.10664808244635919No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGGTGTGGTCGGCA1000.10559216083797938No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCCTCCAGAACAC970.10242439601284002No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACTCGCCCCCTGCC950.10031255279608041No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)