FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109145
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAANGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC15321.4036373631407761No Hit
TGTAAAANGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG14681.3449997709469055No Hit
TGTAAAANGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC14611.338586284300701No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC14121.2936918777772688No Hit
TGTAAAANGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG13671.2524623207659535No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC13641.2497136836318659No Hit
TGTAAAANGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG12091.1077007650373356No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG11811.0820468184525174No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG11611.063722570891933No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG10961.004168766320033No Hit
TGTAAAANGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG10750.9849283063814193No Hit
TGTAAAANGACGGCCAGTTGAGAGGGACTGGAGAAAGGGAACTTACAGAG10720.9821796692473316No Hit
TGTAAAACGACGGCCAGTTGAGAGGGACTGGAGAAAGGGAACTTACAGAG10360.9491960236382794No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG9800.8978881304686427No Hit
TGTAAAANGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC9720.8905584314444088No Hit
TGTAAAANGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA8810.8071831050437491No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC8360.7659535480324339No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA8340.7641211232763755No Hit
TGTAAAANGACGGCCAGTGGGGTGGGGAGTGAGATGGTCCTAGGAAGTAC8200.7512941499839663No Hit
TGTAAAACGACGGCCAGTGGGGTGGGGAGTGAGATGGTCCTAGGAAGTAC7500.6871592835219203No Hit
TGTAAAANGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC7490.6862430711438912No Hit
TGTAAAANGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT7410.6789133721196573No Hit
TGTAAAANGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG7200.6596729121810436No Hit
TGTAAAANGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA7160.6560080626689266No Hit
TGTAAAANGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC7090.6495945760227221No Hit
TGTAAAANGACGGCCAGTTGGATGGTGAGGGAAGGGCTAAGACAGGATTC7010.6422648769984882No Hit
TGTAAAACGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT6990.6404324522424297No Hit
TGTAAAANGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG6940.6358513903522837No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG6800.6230244170598744No Hit
TGTAAAANGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGTGGGAGCCCC6760.6193595675477576No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGTGGGAGCCCC6660.6101974437674653No Hit
TGTAAAANGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGC6600.6047001694992898No Hit
TGTAAAANGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC6580.6028677447432315No Hit
TGTAAAANGACGGCCAGTGGTACGTGGTGGTGGCTGAGAGCCCCCTGCCG6450.5909569838288515No Hit
TGTAAAANGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC6400.5863759219387055No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA6370.5836272848046177No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC6350.5817948600485593No Hit
TGTAAAANGACGGCCAGTCATAGCCCAGGTCCAGCAGCCATCAGGTTCCC6220.5698840991341793No Hit
TGTAAAACGACGGCCAGTTGGATGGTGAGGGAAGGGCTAAGACAGGATTC6200.5680516743781209No Hit
TGTAAAANGACGGCCAGTGGTGTTACCGTGGTCACCCGCAGGGACACGGA6100.5588895505978286No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG6070.5561409134637408No Hit
TGTAAAANGACGGCCAGTGCCCAAGGTTGGTGAGATGGGAACCACCCAGC5990.5488112144395071No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGAGCCCCCTGCCG5940.5442301525493609No Hit
TGTAAAANGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG5870.5378166659031564No Hit
TGTAAAANGACGGCCAGTCTCTCACCCACCTCCACCCATCAAAGGTGCCT5760.5277383297448348No Hit
TGTAAAACGACGGCCAGTGGTGTTACCGTGGTCACCCGCAGGGACACGGA5690.5213248430986303No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGC5630.5158275688304549No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCATCAGGTTCCC5570.5103302945622795No Hit
TGTAAAACGACGGCCAGTCTCTCACCCACCTCCACCCATCAAAGGTGCCT5560.5094140821842503No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC5550.5084978698062211No Hit
TGTAAAANGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG5480.5020843831600165No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC5370.49200604700169503No Hit
TGTAAAANGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC5350.49017362224563654No Hit
TGTAAAANGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC5330.4883411974895781No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC5210.47734664895322737No Hit
TGTAAAANGACGGCCAGTCCACTGCATGGCTACTGAATGCTCACCACAAT5170.47368179944111044No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC5170.47368179944111044No Hit
TGTAAAANGACGGCCAGTCTGTCTGGCATGGGCCTCTCCACCGTGCCTGA5080.4654358880388474No Hit
TGTAAAANGACGGCCAGTACACATTCTTGTAAAGCATGGGATTGCTGAAT4950.45352512712446746No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG4880.4471116404782628No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC4870.44619542810023366No Hit
TGTAAAANGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC4860.4452792157222044No Hit
TGTAAAANGACGGCCAGTCCATTACTGCTGCGTCCCTGTTTTACAATGCA4590.42054148151541526No Hit
TGTAAAACGACGGCCAGTGCCCAAGGTTGGTGAGATGGGAACCACCCAGC4580.419625269137386No Hit
TGTAAAACGACGGCCAGTACACATTCTTGTAAAGCATGGGATTGCTGAAT4570.4187090567593569No Hit
TGTAAAACGACGGCCAGTCTGTCTGGCATGGGCCTCTCCACCGTGCCTGA4560.41779284438132763No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG4530.41504420724723995No Hit
TGTAAAANGACGGCCAGTCCCTGTCCTGAGCACCGTTGCTAACCCCCAGT4510.41321178249118146No Hit
TGTAAAANGACGGCCAGTCCTCACTCCCCACCCAGGATCTGGGCACTGGA4460.4086307206010353No Hit
TGTAAAANGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC4350.3985523844427138No Hit
TGTAAAACGACGGCCAGTCCACTGCATGGCTACTGAATGCTCACCACAAT4340.39763617206468466No Hit
TGTAAAANGACGGCCAGTACTTCCCAGTTTCCAGTCCCCATTTCTCCTTC4320.3958037473086261No Hit
TGTAAAANGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA4320.3958037473086261No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC4310.39488753493059686No Hit
TGTAAAANGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCGTGGGTC4100.37564707499198313No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCATTTCTCCTTC4070.37289843785789545No Hit
TGTAAAANGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC4050.371066013101837No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC4040.37014980072380776No Hit
TGTAAAANGACGGCCAGTAGCAGGCAGGTCCTTCCAGACAGCCAATGAGG3990.36556873883366164No Hit
TGTAAAANGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA3850.35274176554125247No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA3840.3518255531632232No Hit
TGTAAAACGACGGCCAGTCCTCACTCCCCACCCAGGATCTGGGCACTGGA3830.35090934078519403No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC3800.3481607036511063No Hit
TGTAAAANGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG3780.34632827889504786No Hit
TGTAAAACGACGGCCAGTCCCTGTCCTGAGCACCGTTGCTAACCCCCAGT3670.33624994273672637No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT3660.3353337303586972No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC3630.33258509322460944No Hit
TGTAAAANGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA3540.32433918182234645No Hit
TGTAAAANGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT3530.3234229694443172No Hit
TGTAAAANGACGGCCAGTCCGCCCTTTCCAAGCTCACTATTTCCCTACTT3530.3234229694443172No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGTCCCTGTTTTACAATGCA3500.3206743323102295No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCGTGGGTC3500.3206743323102295No Hit
TGTAAAANGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC3490.3197581199322003No Hit
TGTAAAACGACGGCCAGTAGCAGGCAGGTCCTTCCAGACAGCCAATGAGG3460.3170094827981126No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA3410.31242842090796646No Hit
TGTAAAANGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG3360.30784735901782034No Hit
TGTAAAACGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG3220.29502038572541117No Hit
TGTAAAANGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA3180.29135553621329424No Hit
TGTAAAANGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC3140.28769068670117737No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG3080.28219341243300194No Hit
TGTAAAANGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT3010.2757799257867974No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA2970.27211507627468046No Hit
TGTAAAANGACGGCCAGTGGAACTGGTGGGCAGTGTCCCAAGAGGTGGGG2960.2711988638966512No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTATTTCCCTACTT2940.2693664391405928No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCCAAGAGGTGGGG2840.26020431536030053No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT2800.2565394658481836No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG2730.250125979201979No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC2720.2492097668239498No Hit
TGTAAAANGACGGCCAGTGGCTGACGCAGGAGAATTGCTTGGACCCAGAG2720.2492097668239498No Hit
TGTAAAANGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC2680.2455449173118329No Hit
TGTAAAANGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG2670.24462870493380365No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA2640.24188006779971596No Hit
TGTAAAANGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCATTGTGGGCTA2560.23455036877548216No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC2560.23455036877548216No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCATTGTGGGCTA2510.22996930688533604No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTTGGACCCAGAG2360.21622612121489762No Hit
TGTAAAANGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC2340.21439369645883918No Hit
TGTAAAANGACGGCCAGTGACCTCATGGGGCGCTTCCGCGTGCTGGGCTG1980.18141005084978698No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTCCCTTCTCTTTG1950.1786614137156993No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC1940.17774520133767008No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT1940.17774520133767008No Hit
TGTAAAANGACGGCCAGTCAGCAGCAACTCCCTGTGCTCCCTTCTCTTTG1840.1685830775573778No Hit
TGTAAAANGACGGCCAGTCTTGCACTACATGGCCATCAGGGTCTCGTCCC1810.16583444042329012No Hit
TGTAAAANGACGGCCAGTACCTCATGTCACGTGCGTCCATGTGAAGAGAG1790.16400201566723166No Hit
TGTAAAANGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT1780.16308580328920244No Hit
TGTAAAANGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT1750.16033716615511476No Hit
TGTAAAACGACGGCCAGTACCTCATGTCACGTGCGTCCATGTGAAGAGAG1660.1520912547528517No Hit
TGTAAAACGACGGCCAGTTGGCCCACTGTGCTAGCCTAGGATTTTTTTTT1650.15117504237482246No Hit
TGTAAAANGACGGCCAGTTGGCCCACTGTGCTAGCCTAGGATTTTTTTTT1650.15117504237482246No Hit
TGTAAAACGACGGCCAGTGACCTCATGGGGCGCTTCCGCGTGCTGGGCTG1620.1484264052407348No Hit
TGTAAAANGACGGCCAGTGGAACTGGTGGGCAGTGTCCAAGAGGTGGGGT1590.14567776810664712No Hit
TGTAAAANGACGGCCAGTTGATGACCTCTTGGCTAACATACACAACATGA1550.14201291859453022No Hit
TGTAAAANGACGGCCAGTCCCCCAGAAAATTGTGCCAAAGAGTTTAGAAA1530.14018049383847175No Hit
TGTAAAACGACGGCCAGTCCCCCAGAAAATTGTGCCAAAGAGTTTAGAAA1520.13926428146044253No Hit
TGTAAAANGACGGCCAGTCGGTGCTCTGGTGAGATGGGCCCGGATGTTGG1420.13010215768015027No Hit
TGTAAAANGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT1410.12918594530212105No Hit
TGTAAAACGACGGCCAGTCTTGCACTACATGGCCATCAGGGTCTCGTCCC1410.12918594530212105No Hit
TGTAAAACGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT1390.12735352054606258No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT1370.12552109579000412No Hit
TGTAAAANGACGGCCAGTCTGGGCCTTGGCTTCTTCCTCAATCATCTAGG1340.12277245865591645No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCACTGCCTAGCAGG1300.11910760914379953No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCAAGAGGTGGGGT1250.1145265472536534No Hit
TGTAAAANGACGGCCAGTGGGTAGGAAGTCGGGAGGCAGCCCCAGGCAGT1220.1117779101195657No Hit
TGTAAAANGACGGCCAGTGGGTAGGAAGTCGGGAGGCACTGCCTAGCAGG1220.1117779101195657No Hit
TGTAAAACGACGGCCAGTTGATGACCTCTTGGCTAACATACACAACATGA1210.11086169774153648No Hit
TGTAAAANGACGGCCAGTCTTGCACTACATGGCCATCATGAACCAGCGAA1210.11086169774153648No Hit
TGTAAAANGACGGCCAGTTGTGGAAGTCGGGGACATAAGATGATTTTCAC1140.10444821109533191No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGCCCGGATGTTGG1130.10353199871730268No Hit
TGTAAAANGACGGCCAGTGGAACTGGTGGGCAGTGTCCAGCTCTCCAGAG1130.10353199871730268No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)