FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109145
Sequences flagged as poor quality0
Sequence length300
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC9810.8988043428466718No Hit
CAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG9370.8584909982133858No Hit
CAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC9320.8539099363232396No Hit
CAGGAAANAGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC9080.8319208392505382No Hit
CAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT8990.8236749278482752No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT8460.7751156718127262No Hit
CAGGAAANAGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT7980.7311374776673233No Hit
CAGGAAANAGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT7610.6972376196802419No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA7180.6578404874249851No Hit
CAGGAAANAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT6380.5845434971826469No Hit
CAGGAAANAGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG6370.5836272848046177No Hit
CAGGAAANAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC6110.5598057629758578No Hit
CAGGAAANAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG5960.5460625773054194No Hit
CAGGAAANAGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT5870.5378166659031564No Hit
CAGGAAANAGCTATGACCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT5830.5341518163910395No Hit
CAGGAAANAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC5810.532319391634981No Hit
CAGGAAANAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC5690.5213248430986303No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC5480.5020843831600165No Hit
CAGGAAANAGCTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA5380.49292225937972417No Hit
CAGGAAANAGCTATGACCCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT4870.44619542810023366No Hit
CAGGAAANAGCTATGANNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT4590.42054148151541526No Hit
CAGGAAANAGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG4320.3958037473086261No Hit
CAGGAAANAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA4320.3958037473086261No Hit
CAGGAAACAGCTATGACCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT4230.3875578359063631No Hit
CAGGAAANAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT4100.37564707499198313No Hit
CAGGAAANAGCTATGANNTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG4030.3692335883457785No Hit
CAGGAAANAGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG4000.3664849512116909No Hit
CAGGAAANAGCTATGANNCCCAAACCTGCGGAGGCACGGGGATGGCAGTG3990.36556873883366164No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG3980.3646525264556324No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3930.3600714645654863No Hit
CAGGAAANAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3880.35549040267534016No Hit
CAGGAAANAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT3750.3435796417609602No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCCAAACCAG3740.34266342938293093No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC3710.3399147922488433No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC3580.3280040313344633No Hit
CAGGAAANAGCTATGANNCTTGAGCTCCTCGGACACGGCGGCCTTGCTGA3500.3206743323102295No Hit
CAGGAAANAGCTATGANCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC3460.3170094827981126No Hit
CAGGAAANAGCTATGANCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG3460.3170094827981126No Hit
CAGGAAANAGCTATGANNGCAAAACACCTTAACACTCGGCTTCTATTTGC3450.3160932704200834No Hit
CAGGAAANAGCTATGANNAAGTTGGTCAGCCCTGGTGACTCATTGGATAT3430.31426084566402496No Hit
CAGGAAANAGCTATGANCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT3400.3115122085299372No Hit
CAGGAAANAGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT3340.30601493426176185No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG3340.30601493426176185No Hit
CAGGAAANAGCTATGANNTCTGACTGTCCGTGATCCCACACAATCACCCT3310.3032662971276742No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT3290.30143387237161573No Hit
CAGGAAANAGCTATGACCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA3290.30143387237161573No Hit
CAGGAAANAGCTATGANCACAGTGTCCCCATCAGGCTCCGGGACAGCATC3250.2977690228594988No Hit
CAGGAAANAGCTATGANNTCAAGCAAGTTCAGGATGGTATAGAAATAGAG3240.2968528104814696No Hit
CAGGAAANAGCTATGANCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC3240.2968528104814696No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC3220.29502038572541117No Hit
CAGGAAANAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG3170.290439323835265No Hit
CAGGAAANAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC3170.290439323835265No Hit
CAGGAAANAGCTATGANNAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT3130.28677447432314807No Hit
CAGGAAANAGCTATGANNGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA3130.28677447432314807No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA2790.25562325347015435No Hit
CAGGAAANAGCTATGANCCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT2700.24737734206789133No Hit
CAGGAAANAGCTATGANNTGTTGTCTTTCAGGGGCCGCCATGCCTGCCGT2690.24646112968986209No Hit
CAGGAAACAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT2670.24462870493380365No Hit
CAGGAAANAGCTATGANNGCCCCTACTCCAAAAGGCGGATGAATGGGGAA2640.24188006779971596No Hit
CAGGAAANAGCTATGANCCAGGGCTCCAAGAATGTTCCACACATCTTTCT2630.24096385542168677No Hit
CAGGAAANAGCTATGACCCTTGAGCTCCTCGGACACGGCGGCCTTGCTGA2620.24004764304365753No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA2520.23088551926336523No Hit
CAGGAAANAGCTATGANCAGGCACCAACAGTACAAAGCAGATACAAAGCA2500.2290530945073068No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG2480.22722066975124833No Hit
CAGGAAANAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC2480.22722066975124833No Hit
CAGGAAANAGCTATGANNAGCAAAGGTCTTGGCAGGGGTTCTCCTTCCCT2480.22722066975124833No Hit
CAGGAAANAGCTATGACCTGTTTTGCCCCTGAAATGAATATCCAAACCAG2480.22722066975124833No Hit
CAGGAAANAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG2460.22538824499518986No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG2440.2235558202391314No Hit
CAGGAAANAGCTATGANNTGGGTTATGGCGTGGGATGCCCTGCAACCCCC2430.2226396078611022No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT2430.2226396078611022No Hit
CAGGAAANAGCTATGANCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG2420.22172339548307296No Hit
CAGGAAACAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT2420.22172339548307296No Hit
CAGGAAANAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT2410.22080718310504374No Hit
CAGGAAANAGCTATGANNCAGGGTGACTCCGATCCTGGAGGACAAGGCGG2390.2189747583489853No Hit
CAGGAAANAGCTATGACCTGTTTTGCCCCTGAAATGAATATCTAAACCAG2370.21714233359292684No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG2330.2134774840808099No Hit
CAGGAAANAGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC2310.21164505932475147No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG2300.21072884694672225No Hit
CAGGAAANAGCTATGANNAGACAGGGTCTTGCTATGTTGCCCAGGCTGGT2250.2061477850565761No Hit
CAGGAAANAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT2150.19698566127628386No Hit
CAGGAAANAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG2150.19698566127628386No Hit
CAGGAAANAGCTATGANCCTGGAATTTCAACAGAATGGCATTTATATGAT2110.19332081176416693No Hit
CAGGAAANAGCTATGACCCCCAAACCTGCGGAGGCACGGGGATGGCAGTG2110.19332081176416693No Hit
CAGGAAANAGCTATGANCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2100.1924045993861377No Hit
CAGGAAANAGCTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC2090.19148838700810847No Hit
CAGGAAANAGCTATGANNACCTGGCAGCCCCAAATCTGCACCTCCCGCAG2050.18782353749599157No Hit
CAGGAAANAGCTATGANNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG2040.18690732511796235No Hit
CAGGAAANAGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC2040.18690732511796235No Hit
CAGGAAANAGCTATGANNGCTGCTGACCCAACCCTACCCTGTCCAGCCCC2040.18690732511796235No Hit
CAGGAAANAGCTATGANNGGGCAGGCCGAGGACATTAGAAATTTGGGACG2030.18599111273993313No Hit
CAGGAAANAGCTATGACCTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG1990.1823262632278162No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTATGTGCA1980.18141005084978698No Hit
CAGGAAANAGCTATGANNTCCCTTAAACTTGGGATTGCTCCATTGCACTC1980.18141005084978698No Hit
CAGGAAANAGCTATGANNACCTGGCAGCCCCAAATCTGCACCTCCCGCGG1970.18049383847175776No Hit
CAGGAAANAGCTATGANNTGGGTTGCCTGGGACTGAGAGGGTCCTGGGAC1950.1786614137156993No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA1940.17774520133767008No Hit
CAGGAAANAGCTATGACCAGCAAAGGTCTTGGCAGGGGTTCTCCTTCCCT1920.1759127765816116No Hit
CAGGAAANAGCTATGACCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG1880.1722479270694947No Hit
CAGGAAANAGCTATGACCTCTGACTGTCCGTGATCCCACACAATCACCCT1870.17133171469146546No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1870.17133171469146546No Hit
CAGGAAANAGCTATGANNTCCAGTGGTGGGGCTCAACAAAGGCTCCGTGA1820.16675065280131934No Hit
CAGGAAANAGCTATGANNCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG1780.16308580328920244No Hit
CAGGAAANAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT1750.16033716615511476No Hit
CAGGAAANAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT1690.1548398918869394No Hit
CAGGAAANAGCTATGANCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA1670.15300746713088093No Hit
CAGGAAANAGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT1670.15300746713088093No Hit
CAGGAAANAGCTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC1660.1520912547528517No Hit
CAGGAAANAGCTATGANNTCCAGTGGTGGGGCTCAACAAAGGCTCTGTGA1640.15025882999679327No Hit
CAGGAAANAGCTATGANCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT1620.1484264052407348No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCCAAACCAG1600.14659398048467637No Hit
CAGGAAANAGCTATGACCGCCCCTACTCCAAAAGGCGGATGAATGGGGAA1590.14567776810664712No Hit
CAGGAAANAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA1590.14567776810664712No Hit
CAGGAAACAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG1580.1447615557286179No Hit
CAGGAAANAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG1570.14384534335058868No Hit
CAGGAAANAGCTATGACCTCCCTTAAACTTGGGATTGCTCCATTGCACTC1500.13743185670438407No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC1500.13743185670438407No Hit
CAGGAAANAGCTATGACCCACGACACCCCACCTCTTGGGACACTGCCCAC1490.13651564432635485No Hit
CAGGAAANAGCTATGANCGCAAAACACCTTAACACTCGGCTTCTATTTGC1490.13651564432635485No Hit
CAGGAAANAGCTATGACCTGTTGTCTTTCAGGGGCCGCCATGCCTGCCGT1490.13651564432635485No Hit
CAGGAAANAGCTATGANNTGCTGCCGAGAGAGGACAGTGATCCCCCAAAA1480.1355994319483256No Hit
CAGGAAANAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA1460.13376700719226717No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACGGGGATGGCAGTG1450.13285079481423792No Hit
CAGGAAACAGCTATGACCTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG1440.1319345824362087No Hit
CAGGAAANAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG1430.13101837005817948No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGCGGCCTTGCTGA1420.13010215768015027No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCTAAACCAG1420.13010215768015027No Hit
CAGGAAANAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC1390.12735352054606258No Hit
CAGGAAANAGCTATGANCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG1380.12643730816803336No Hit
CAGGAAANAGCTATGANCCCCAAACCTGCGGAGGCACGGGGATGGCAGTG1370.12552109579000412No Hit
CAGGAAANAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA1370.12552109579000412No Hit
CAGGAAANAGCTATGANNGGTAGCAACGTCCCACCCAGCTGAATAGTTGT1370.12552109579000412No Hit
CAGGAAANAGCTATGANNAGAAAGAAGAAGCAGAGCAAAAACAATAGTCT1340.12277245865591645No Hit
CAGGAAANAGCTATGANCTGTTTTGCCCCTGAAATGAATATCCAAACCAG1340.12277245865591645No Hit
CAGGAAANAGCTATGANNGCTACGGGCAATCACTCTCCTATTCCCAACCA1320.12094003389985798No Hit
CAGGAAACAGCTATGACCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG1320.12094003389985798No Hit
CAGGAAANAGCTATGANCTGTTTTGCCCCTGAAATGAATATCTAAACCAG1320.12094003389985798No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG1310.12002382152182876No Hit
CAGGAAANAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG1310.12002382152182876No Hit
CAGGAAANAGCTATGANCTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG1310.12002382152182876No Hit
CAGGAAANAGCTATGANNGCTACGGGCAATCACTCTCCTACTCCCAACCA1300.11910760914379953No Hit
CAGGAAANAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT1290.1181913967657703No Hit
CAGGAAANAGCTATGANCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG1280.11727518438774108No Hit
CAGGAAACAGCTATGACCTCTGACTGTCCGTGATCCCACACAATCACCCT1230.11269412249759493No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA1220.1117779101195657No Hit
CAGGAAANAGCTATGANNAGAAAGAAGAAGCAGAGCAAAAACAGTAGTCT1220.1117779101195657No Hit
CAGGAAANAGCTATGACCAGACAGGGTCTTGCTATGTTGCCCAGGCTGGT1210.11086169774153648No Hit
CAGGAAANAGCTATGANNGCCCTGGCCACTGTGGTTAATGAAACTTGGTT1210.11086169774153648No Hit
CAGGAAANAGCTATGANCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT1200.10994548536350728No Hit
CAGGAAANAGCTATGANCCTTGAGCTCCTCGGACACGGCGGCCTTGCTGA1200.10994548536350728No Hit
CAGGAAANAGCTATGANNGGTAGCAACGTCCCACCCAGGGCACGGGAACA1190.10902927298547804No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA1190.10902927298547804No Hit
CAGGAAANAGCTATGACCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG1180.10811306060744881No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT1180.10811306060744881No Hit
CAGGAAANAGCTATGANCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG1180.10811306060744881No Hit
CAGGAAANAGCTATGANCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT1180.10811306060744881No Hit
CAGGAAANAGCTATGANNAGAGGCACAGGTGGAGAGGGACGAGACCCTGA1170.10719684822941959No Hit
CAGGAAANAGCTATGACCGGGCAGGCCGAGGACATTAGAAATTTGGGACG1160.10628063585139036No Hit
CAGGAAANAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC1160.10628063585139036No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGTTCTCCTTCCCT1150.10536442347336113No Hit
CAGGAAANAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC1150.10536442347336113No Hit
CAGGAAACAGCTATGACCGCCCCTACTCCAAAAGGCGGATGAATGGGGAA1130.10353199871730268No Hit
CAGGAAANAGCTATGANNCACGACACCCCACCTCTTGGACACTGCCCACC1120.10261578633927344No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT1120.10261578633927344No Hit
CAGGAAANAGCTATGANCTGGGGATGAGGACAGCAGGGCAGTCACCATCT1120.10261578633927344No Hit
CAGGAAANAGCTATGACCTGGGTTGCCTGGGACTGAGAGGGTCCTGGGAC1100.10078336158321499No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)