FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003246419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27432
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA578021.070282881306504No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA5251.9138232720909885No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA3661.3342082239720034No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG3471.2649460484106154No Hit
TGTAAAACGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG2961.079031787693205No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCATTGTGGGCTA2761.006124234470691No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG2740.9988334791484399No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA2600.9477981918926801No Hit
TGTAAAACGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT2420.8821813939924176No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC2360.8603091280256634No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC2350.8566637503645378No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC1870.6816856226305046No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG1820.6634587343248761No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG1780.6488772236803733No Hit
TGNAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAATCTCCACGCAGGA1740.6342957130358705No Hit
TGTAAAACGACGGCTAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA1680.6124234470691163No Hit
TGTAAAACGACGGCCAGTGCCACACGAGGCTTCATTCGTTATGGCTGGCG1520.5540974044911053No Hit
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG1440.5249343832020997No Hit
TGTAAAACGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA1430.521289005540974No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAACACCTCCACGCAGGA1300.47389909594634005No Hit
TGTAAAACGACGGCCAGTAGTGACAACAGCCCCAAAGGAATGATCCCGCT1180.4301545640128317No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC1180.4301545640128317No Hit
TGTAAAACGACGGCCAGTCCAGAGATCAATAATAGCTCAGGGGAGATGGA1170.42650918635170604No Hit
TGTAAAACGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT1160.4228638086905804No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC1140.41557305336832895No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC1140.41557305336832895No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC1130.41192767570720323No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG1090.3973461650627005No Hit
TGTAAAACGACGGCCAGTGGCTCTCATCCTCTTCTGCAACTACTATGTTT1080.39370078740157477No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGTAAACCTCCACGCAGGA1040.379119276757072No Hit
TGTAAAACGACGGCCAGTAACAAATTCAGGTGAGATGCGCTCATATAGAA930.3390201224846894No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG920.3353747448235637No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG900.32808398950131235No Hit
TGTAAAACGACGGCCAGTTTGACCGTCCCACCTGCCTTGGTTTTCCCTTT890.32443861184018663No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG770.28069407990667833No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT730.2661125692621756No Hit
TGTAAAACGACGGCCAGTGGATGCCAAGGCAGTACCTGAAGCACCAGGGA730.2661125692621756No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG730.2661125692621756No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG700.2551764362787985No Hit
TGTAAAACGACGGCCAGTAGCACGAAAAGTAGGTGGCCTTACTGGTAGCA700.2551764362787985No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG680.2478856809565471No Hit
TGTAAAACGACGGCCAGTTGGAGTGCCTGGGTTAGAACCCTGCTGGTACC670.2442403032954214No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG640.23330417031204434No Hit
TGTAAAACGACGGCCAGTCCTGTGCCCCAGCCTCCTTAATGTGTGCACTC630.22965879265091863No Hit
TGTAAAACGACGGCCAGTTGACACAGCTGCTTCTGAGTACAAGTATGTAG620.22601341498979297No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCAGTGGTGCGATGGTCATAGCTG600.21872265966754156No Hit
TGTAAAACGACGGCCAGTCGCCTGAATAGGTGCAGGGGCACGAGCTCGGC580.2114319043452902No Hit
TGTAAAACGACGGCCAGTGGAGCGACTTGACAATGCCCCTCACACCCCTT560.2041411490230388No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC560.2041411490230388No Hit
TGTAAAACGACGGCCAGTTCCTGGGCTTCTCTTGAGTACGGAGGAAAGTG550.20049577136191313No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC520.189559638378536No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG510.18591426071741032No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT510.18591426071741032No Hit
TGTAAAACGACGGCCAGTCCTGTGTTTCCACTTCTGCTTCCAAAGATTTT510.18591426071741032No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC510.18591426071741032No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT500.18226888305628464No Hit
TGTAAAACGACGGCCAGTGGATGCCAAGGCAGTACCTGAAGCACCGGGGA500.18226888305628464No Hit
TGTAAAACGACGGCCAGTACACTCCCTCCACCTCCCATCCCAGAAAAAGT490.17862350539515895No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT490.17862350539515895No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC470.17133275007290755No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCCGGA450.16404199475065617No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC440.16039661708953046No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC430.15675123942840477No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA420.15310586176727908No Hit
GTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGAG410.1494604841061534No Hit
TGTAAAACGACGGCCAGTCGCAGGTTTGTGAAGAAAACCTCCACGCAGGA410.1494604841061534No Hit
TGTAAAACGACGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGAG400.1458151064450277No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCCGTGGG400.1458151064450277No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG400.1458151064450277No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG390.14216972878390202No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC380.13852435112277633No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGGCTTCTGGGCTT380.13852435112277633No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAGACCTCCACGCAGGA370.13487897346165062No Hit
TGTAAAACGACGGCCAGTCCTGCCGCCTGGTCTCACCTCGCTATGGTTCG370.13487897346165062No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGC360.13123359580052493No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA350.12758821813939925No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT340.12394284047827354No Hit
TGTAAAACGACGGCCAGTCGCCTGAATAGGTGCAGGGGTGTGATCATGGC330.12029746281714784No Hit
TGTAAAACGACGGCCAGTCCTGCCGCCTGGTCTCACCTCGCCATGGTTCG330.12029746281714784No Hit
TAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGAGT310.11300670749489648No Hit
TGTAAAACGACGGCCAGTCGCCGGTGTGTGAAGAAAACCTCCACGCAGGA300.10936132983377078No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGGGGAGGATCCTC300.10936132983377078No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA300.10936132983377078No Hit
TGTAAAACGACGGCCAGTCAGCCAGTAGCAACAGCAGCTACTGATGTAAG290.1057159521726451No Hit
TGTAAAACGAGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGAG290.1057159521726451No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCTGGAGTGCAGTG280.1020705745115194No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG280.1020705745115194No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC280.1020705745115194No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)