FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003246420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27432
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG21797.943277923592884No Hit
CAGGAAANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG7012.5554097404491105No Hit
NAGGAAANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG2150.7837561971420239No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1990.7254301545640128No Hit
CAGGANANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1950.71084864391951No Hit
NAGGANANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1850.6743948673082532No Hit
CAGGAAACAGCNATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1440.5249343832020997No Hit
CAGGAAACAGCTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG1400.510352872557597No Hit
CAGGAAACNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1360.4957713619130942No Hit
CAGGAANNNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1110.4046369203849519No Hit
NAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG980.3572470107903179No Hit
CAGGAAANAGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT900.32808398950131235No Hit
NAGGAAANAGCTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG860.31350247885680954No Hit
CAGGAAANAGCTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC830.3025663458734325No Hit
CAGGAAANAGCTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA770.28069407990667833No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT740.26975794692330124No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA740.26975794692330124No Hit
CAGGAAANAGCTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA740.26975794692330124No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG720.26246719160104987No Hit
CAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC600.21872265966754156No Hit
CAGGAAANAGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT600.21872265966754156No Hit
CAGGAAANAGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT570.20778652668416447No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA550.20049577136191313No Hit
CAGGAAANAGCTATGANNCCCGTGACAGCTCCTGCAATTACAAAATGGCT520.189559638378536No Hit
CAGGAAANAGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC480.17497812773403326No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT470.17133275007290755No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT460.16768737241178186No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA460.16768737241178186No Hit
CAGGACANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG440.16039661708953046No Hit
CAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA420.15310586176727908No Hit
CAGGAAANAGCTATGANNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT410.1494604841061534No Hit
CCGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG410.1494604841061534No Hit
CAGGAAANAGCTATGANNCGACATATCCGAGCCTCCCGTTCTAGTTTCTG390.14216972878390202No Hit
CAGGAAANNGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT390.14216972878390202No Hit
CAGGAAANAGCTATGANNGGGGAGACTGGGCCATACGATAGGCTTAATCC390.14216972878390202No Hit
CAGGCAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG360.13123359580052493No Hit
CAGGAAANNGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT360.13123359580052493No Hit
CAGGAAANAGCTCTGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG350.12758821813939925No Hit
CAGGAAANAGCTATGANNTCCTCTTCATTTTCACGGTGCCATTCTGATTC320.11665208515602217No Hit
CAGGAAANNGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA310.11300670749489648No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA300.10936132983377078No Hit
CAGGAAANNGCTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA300.10936132983377078No Hit
CAGGAAANAGCTATGANNTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG290.1057159521726451No Hit
CAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGCTCTAGTTTCTG290.1057159521726451No Hit
CAGGAAANAGCTATGANNCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC290.1057159521726451No Hit
CAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTCGTTTCTG280.1020705745115194No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)