FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109196
Sequences flagged as poor quality0
Sequence length300
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCTGGAAACCCATGCCATGCACTCAACACTACAA46504.258397743507088No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGCACTCCTTC34863.1924246309388624No Hit
CAGGAAACAGCTATGACCGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC28802.637459247591487No Hit
CAGGAAACAGCTATGACCTGACTGAAAGGCTTTTCCACGTGCACTGCTGG25172.305029488259643No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT24792.2702296787428113No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACCCTGTCTCTCTAGC21271.9478735484816294No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC17461.5989596688523389No Hit
CAGGAAACAGCTATGACCGCTAATGCTTACTCTTTGCGTGAGTACTGGTT17221.5769808417890767No Hit
CAGGAAACAGCTATGACCACCTTTACCCAAATAGCTACCACCAGTGCCTT15031.3764240448368072No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGGCTGTTT15021.375508260375838No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG13441.2308143155426938No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC13431.2298985310817245No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCACCCCACCCTGCT13311.2189091175500935No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATGGTGAGCCCTGC12561.1502252829773985No Hit
CAGGAAACAGCTATGACCGCAGCCACCCTGAACACACTGGGCAGGAAGAA11331.0375837942781787No Hit
CAGGAAACAGCTATGACCCTGGGTGCCCTAGACCCAGTATCCAAGGCTCA10690.9789735887761457No Hit
CAGGAAACAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT10250.9386790724934979No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA9400.8608373933111103No Hit
CAGGAAACAGCTATGACCGGCGGGCCCTGATATAGAGCCACTGGCCGTCG9260.8480164108575405No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACCACCGTGATCTCTC8920.8168797391845855No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAACCATGTGCCC8780.8040587567310157No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG8610.7884904208945384No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT8410.7701747316751529No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG8280.7582695336825525No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGGTCCTCCTC8100.7417854133851056No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA7890.7225539397047511No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGCTCAAGG7770.7115645261731198No Hit
CAGGAAACAGCTATGACCATCCACTGTTGGCCAACACAGGGGCGATAGTG7320.670354225429503No Hit
CAGGAAACAGCTATGACCCCGTAGCCACGCCCCTATTGTCTGACCACGCT7170.6566174585149639No Hit
CAGGAAACAGCTATGACCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT6890.6309754936078245No Hit
CAGGAAACAGCTATGACCAGTGTGAAGTCTGGATCAAATATTGCCAGGAA6830.6254807868420089No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGGCTGCTT6640.6080808820835928No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCCTCCCCACCTCC6400.5861020550203304No Hit
CAGGAAACAGCTATGACCATCCACTGTTGGCCAACACACCTGGCCTTCAA6350.5815231327154841No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGCTCCTCCTCGC5970.5467233231986519No Hit
CAGGAAACAGCTATGACCGTCCTAGCACCGCAGCGAATGGGACCCGCAAG5920.5421444008938057No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATGGAGCCCTGCAG5740.5256602805963588No Hit
CAGGAAACAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT5550.5082603758379428No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGCCCGGACGAGGA5490.5027656690721272No Hit
CAGGAAACAGCTATGACCTGGGCCTGGACACTTTGACGGCGGGGCACGTC5410.49543939338437304No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT5260.4817026264698341No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCAGCTCCGA5100.46705007509432583No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC4830.4423238946481556No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCTTCCCATCTCA4820.44140811018718634No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCCAAACCAG4810.44049232572621705No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTATTGCAGCCTGT4650.4258397743507088No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT4620.423092420967801No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC4610.4221766365068318No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTGGGGAGGGTGG4530.4148503608190776No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC4530.4148503608190776No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCTCTTTTAGG4520.4139345763581084No Hit
CAGGAAACAGCTATGACCGAGGCGCAGGTCACAGTGAGACCAATACAGAG4520.4139345763581084No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG4460.4084398695922927No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTGGCAGCTGCAC4260.3901241803729074No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAGGCACTACGT4250.3892083959119382No Hit
CAGGAAACAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG4180.3827979046851533No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG4080.37364006007546063No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTCCCCAACCATAG4060.3718084911535221No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACTGCCTCCCGACT4040.36997692223158357No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGTCTGCAGCCTGG3780.3461665262463826No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT3710.3397560350195978No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG3700.3388402505586285No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT3670.3360928971757207No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTACGTGTCCCTAG3660.3351771127147515No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGATACTACTA3650.3342613282537822No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT3610.3305981904099051No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAACTGGCCAGCCTG3570.32693505256602806No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACCCCGTTAGCTGC3540.32418769918312024No Hit
CAGGAAACAGCTATGACCCCTGACCCCTTCTTCTCCAGGATGTAAAACTT3480.31869299241730464No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTATTGAGGCACTT3450.3159456390343969No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG3400.3113667167295505No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGCATCCTGG3360.30770357888567346No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCTAAACCAG3230.29579838089307303No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACCAGGAAACAGCT3170.2903036741272574No Hit
CAGGAAACAGCTATGACCAAAGGCAGCAGGGTCGATGGTGGGGGGCTGGC3120.2857247518224111No Hit
CAGGAAACAGCTATGACCGGCCTTCTCTGCATTATCTCGCTAATCTCGAG3120.2857247518224111No Hit
CAGGAAACAGCTATGACCGCAGCCACCCTGAACACACTCCACCAGGAAGA3090.28297739843950326No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGTGCTGCCTCTCCA3070.2811458295175648No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCTCTCCACGTGC3060.2802300450565955No Hit
CAGGAAACAGCTATGACCTGCTGCTTTTCCTGCCAGAAGACAGAAGCCCA3050.2793142605956262No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT3030.27748269167368766No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATCCGAGTGAGGAG2980.27290376936884136No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGTGGGGACTGGAA2860.2619143558372102No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGGTGGGGACTGGA2800.25641964907139453No Hit
CAGGAAACAGCTATGACCGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG2660.2435986666178248No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGCCTGGCTTGTC2600.23810395985200922No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGCTGGGGACTGGA2570.23535660646910145No Hit
CAGGAAACAGCTATGACCTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG2500.22894611524231656No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT2460.22528297739843947No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTAGTGGCATGAA2390.21887248617165467No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCACCCTGCTTGTGT2360.21612513278874684No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTGGCATGAAGGA2360.21612513278874684No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT2270.20788307264002345No Hit
CAGGAAACAGCTATGACCTCCCTTAAACTTGGGATTGCTCCATTGCACTC2270.20788307264002345No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTGTTCTGGAATCC2230.20421993479614636No Hit
CAGGAAACAGCTATGACCAGAGGCACAGGTGGAGAGGGACGAGACCCTGA2220.2033041503351771No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTATGTGACT2200.2014725814132386No Hit
CAGGAAACAGCTATGACCAGGTTGCTGGATTCAGTTTGCTGACCAACTTT2200.2014725814132386No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG2190.20055679695226933No Hit
CAGGAAACAGCTATGACCACTCCCTTGAGCCTTTGGGGGCCTGGAGGGAG2110.19323052126451518No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCTGCCCAACACCC2090.19139895234257664No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCAGGAAACAGCT2060.18865159895966885No Hit
CAGGAAACAGCTATGACCTCTGACTGTCCGTGATCCCACACAATCACCCT2040.18682003003773032No Hit
CAGGAAACAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG2010.18407267665482252No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA2000.18315689219385325No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAATGAAACTTGGTT2000.18315689219385325No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTTGGGCCAGGGT1990.182241107732884No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACCCTTTGTCCA1990.182241107732884No Hit
CAGGAAACAGCTATGACCGTCAACAGCATACCTGTACACCTGAGTGTTCC1980.18132532327191472No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCTGGGAAAAAGGG1910.17491483204512986No Hit
CAGGAAACAGCTATGACCACCTTTACCCAAATAGCTACCACCACTGGCAA1900.1739990475841606No Hit
CAGGAAACAGCTATGACCTGACTGAAAGGCTTTTCCACGTGCACTACTGG1880.17216747866222207No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGGGGGCCTGGCT1850.16942012527931424No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATCCAGCAGCAGAG1820.16667277189640647No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACCAGTGAGCGTA1780.16300963405252938No Hit
CAGGAAACAGCTATGACCACCCAGTTAGAGCCATGGTTCCTGTAGGCTAG1780.16300963405252938No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAGTGCCCCACTG1730.15843071174768306No Hit
CAGGAAACAGCTATGACCCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC1720.1575149272867138No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACCTCCAACCCCGT1630.1492728671379904No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGCGATCTCGTCTC1630.1492728671379904No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGACAAGTGCATGG1590.14560972929411334No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACCTGCCTCCCGAC1560.14286237591120554No Hit
CAGGAAACAGCTATGACCTGCTGCTTTTCCTGCCAGAAGCCCAGCCTTCA1550.14194659145023628No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTAGCAGCAGTGG1550.14194659145023628No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCACCCTACCCTGCT1500.13736766914538995No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTTGGCATGAAGG1440.13187296237957433No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGTCTGTGGGCGGA1420.13004139345763582No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAACCAGGAAACAGC1390.12729404007472803No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTGAGGAGGGTGG1390.12729404007472803No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGATGCCACG1380.12637825561375876No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG1360.12454668669182022No Hit
CAGGAAACAGCTATGACCCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG1350.12363090223085095No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACCATCCTGCCTCC1330.1217993333089124No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCGCCCAACACCC1330.1217993333089124No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCCCCTCCCAACT1320.12088354884794315No Hit
CAGGAAACAGCTATGACCTTGGACAGCACCTTGAATGCCTGGGTTCTAAC1280.11722041100406609No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCACCCCCCCATC1280.11722041100406609No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGTTGGGGACTGGA1250.11447305762115828No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCTCCCCACCTCCT1240.11355727316018903No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGCTGAATAGTTGT1230.11264148869921974No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTTACGGAGGCACT1210.11080991977728122No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAAACCCCATCTCTA1210.11080991977728122No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACTCGCCCCCTGCC1200.10989413531631197No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGCCGGGACGCCCC1200.10989413531631197No Hit
CAGGAAACAGCTATGACCTGCTGCTTTTCCTGCCAGAACAGGAAACAGAA1190.10897835085534267No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGACACACCACT1180.10806256639437342No Hit
CAGGAAACAGCTATGACCAAAGGCAGCAGGGTCGATGGGTGGACGAATGG1170.10714678193340416No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTCACACCTGTAA1170.10714678193340416No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGCTGTCCTCCTC1150.10531521301146561No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTGATCAGGGTGG1130.1034836440895271No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGCAGGTCGTGCTG1120.10256785962855781No Hit
CAGGAAACAGCTATGACCGGCCTTCTCTGCATTATCTCGCTCAGGGAAAG1110.10165207516758855No Hit
CAGGAAACAGCTATGACCGGCCTTCTCTGCATTATCTCGCTAGTCTCGAG1100.1007362907066193No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)