FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77660
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT2850.3669842904970384No Hit
NNGGANNNNGNTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT2240.28843677568890036No Hit
NNGGANNNNGNTATGNNNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT1930.24851918619624003No Hit
NNGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1820.2343548802472315No Hit
NNGGANNNNGNTATGNNNCCACCCTCATGCCTAACCCCAGCACAGGGAGT1730.22276590265258822No Hit
NNGGANNNNGNTATGNNNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1690.21761524594385787No Hit
NAGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT1660.21375225341231005No Hit
NNGGANNNNGNTATGNNNAGTGTGAAGTCTGGATCAAATATTGCCAGGAA1600.20602626834921456No Hit
NNGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1590.20473860417203193No Hit
NNGGANNNNGNTATGNNNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1580.20345093999484934No Hit
NNGGANNNNGNTATGNNNACCGTTAAGCCACACCAAATGGTGAGCCCTGC1540.19830028328611898No Hit
NNGGANNNNGNTATGNNNGGGTCCCACCCATGCTGAAATGTGATCCTCAA1470.18928663404584084No Hit
NNGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGGACACTGCCCAC1450.18671130569147568No Hit
NNGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1420.1828483131599279No Hit
NNGGANNNNGNTATGNNNAGGACTTGCCCACTCTCCCAGGAAGCAGAAGT1410.1815606489827453No Hit
NNGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG1340.17254699974246715No Hit
NNGGANNNNGNTATGANNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT1280.16482101467937163No Hit
NNGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC1260.16224568632500644No Hit
NNGGANNNNGNTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC1210.1558073654390935No Hit
NNGGANNNNGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1200.1545197012619109No Hit
NNGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1190.1532320370847283No Hit
NNGGANNNNGNTATGANNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG1190.1532320370847283No Hit
NNGGANNNNGNTATGNNNGAGGCGCAGGTCACAGTGAGACCAATACAGAG1170.15065670873036313No Hit
NNGGANNNNGNTATGNNNTGAGTTAACGTGATTTAGGACCCTGGCTGTTT1130.14550605202163278No Hit
NNGGANNNNGNTATGANNACCTGGCAGCCCCAAATCTGCGGGTGACCACG1110.14293072366726756No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1100.141643059490085No Hit
NNGGANNNNGNTATGNNNGCTGCTGACCCAACCCTACCTCTGCAGCTAGC1090.1403553953129024No Hit
NNGGANNNNGNTATGNNNTAGAGGACACCCAAGGCCACCCGTCCTCGGAC1080.1390677311357198No Hit
NNGGANNNNGNTATGNNNACCTGGCAGCCCCAAATCTGCGGGTGACCACG1070.13778006695853723No Hit
NAGGANNNNGNTATGNNNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT1050.13520473860417204No Hit
NNGGANNNNGNTATGANNGGGTCCCACCCATGCTGAAATGTGATCCTCAA1040.13391707442698944No Hit
NNGGANNNNGNTATGNNNCAGGGTGACTCCGATCCTGGAGCCAACGGTGC1030.13262941024980685No Hit
NNGGANNNNGNTATGANNACCGTTAAGCCACACCAAATGGTGAGCCCTGC1000.12876641771825909No Hit
NNGGANNNNGNTATGANNTGAGTTAACGTGATTTAGGACCCTGGCTGTTT1000.12876641771825909No Hit
NNGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCAGGAAACAGCTA1000.12876641771825909No Hit
NNGGANNNNGNTATGANNCCACCCTCATGCCTAACCCCAGCACAGGGAGT1000.12876641771825909No Hit
NNGGANNNNGNTATGNNNCTCGTGCAGTCTGGTGTCCGCAGGAAACAGCT990.1274787535410765No Hit
NNGGANNNNGNTATGNNNTGCCAGTCTTCCCCTTCCCTCAGGAATCATGC990.1274787535410765No Hit
NAGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT970.12490342518671131No Hit
NNGGANNNNGNTATGANNAGTGTGAAGTCTGGATCAAATATTGCCAGGAA960.12361576100952872No Hit
NNGGANNNNGNTATGANNGCTGCTGACCCAACCCTACCTCTGCAGCTAGC920.11846510430079836No Hit
NNGGANNNNGNTATGNNNACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA920.11846510430079836No Hit
NNGGANNNNGNTATGNNNAGAGCTACTTCTGTGCCCACCAAAGACGGACT920.11846510430079836No Hit
NNGGANNNNGNTATGANNAGGACTTGCCCACTCTCCCAGGAAGCAGAAGT910.11717744012361575No Hit
NNGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGACAGGAAACA910.11717744012361575No Hit
NNGGANNNNGNTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC910.11717744012361575No Hit
NNGGANNNNGNTATGNNNGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA900.11588977594643318No Hit
NNGGANNNNGNTATGNNNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG900.11588977594643318No Hit
NNGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA870.11202678341488541No Hit
NNGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGACACTGCCCACC860.1107391192377028No Hit
NNGGANNNNGNTATGNNNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA860.1107391192377028No Hit
NAGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC850.10945145506052023No Hit
NNGGANNNNGNTATGNNNCCCAAACCTGCGGAGGCACACAGCTGGGAGGC850.10945145506052023No Hit
NNGGANNNNGNTATGNNNTAGAGGACACCCAAGGCCACGGAGATCCAGCC840.10816379088333762No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC840.10816379088333762No Hit
NNGGANNNNGNTATGNNNCCTCCGTCCCAGCCTGATTTCCTGGGTGTCTG840.10816379088333762No Hit
NAGGANNNNGNTATGNNNGCTGCTGACCCAACCCTACCTCTGCAGCTAGC830.10687612670615503No Hit
NAGGANNNNGNTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT830.10687612670615503No Hit
NNGGANNNNGNTATGANNCAGGGTGACTCCGATCCTGGAGCCAACGGTGC820.10558846252897244No Hit
NNGGANNNNGNTATGANNCTCGTGCAGTCTGGTGTCCGCAGGAAACAGCT820.10558846252897244No Hit
NNGGANNNNGNTATGANNTGCACACACCTCCTATGTTAACAGGCACAAGT820.10558846252897244No Hit
NAGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG820.10558846252897244No Hit
NNGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC810.10430079835178985No Hit
NNGGANNNNGNTATGANNAACACGGCAGTCAGAGGTGCAGGAAACAGCTA810.10430079835178985No Hit
NNGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGAGGGGTATAT810.10430079835178985No Hit
NNGGANNNNGNTATGNNNTGCTGCCGAGAGAGGACAGTCCCCAACCATAG800.10301313417460728No Hit
NNGGANNNNGNTATGNNNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT790.10172546999742467No Hit
NAGGANNNNGNTATGNNNAGGACTTGCCCACTCTCCCAGGAAGCAGAAGT780.10043780582024207No Hit
NNGGANNNNGNTATGNNNGATGGCAGAATGGAAACATGGCAGCTGCCTTG780.10043780582024207No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)