FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003246432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214045
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT46412.1682356513817185No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT44882.0967553551823217No Hit
CAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG44782.092083440398047No Hit
CAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC40671.900067742764372No Hit
CAGGAAANAGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA30631.4310074984232288No Hit
CAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC23971.119857973790558No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA23221.0848186129085007No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT22701.0605246560302741No Hit
CAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT22271.0404354224578944No Hit
CAGGAAANAGCTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA20050.936718914247004No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC19380.9054170851923661No Hit
CAGGAAANAGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT19300.9016795533649467No Hit
CAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT16880.7886192155855077No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG16510.7713331308836927No Hit
CAGGAAANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC16200.7568501950524422No Hit
CAGGAAANAGCTATGANNAGCGTCTCATTCCATAAAGGCCACCATGAAAC16050.7498423228760307No Hit
CAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG15560.7269499404330865No Hit
CAGGAAANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG15460.7222780256488122No Hit
CAGGAAANAGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT14600.6820995585040529No Hit
CAGGAAANAGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC12660.5914644116891308No Hit
CAGGAAANNGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA12040.5624985400266299No Hit
CAGGAAANAGCTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA10930.5106402859211848No Hit
CAGGAAANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC10070.4704618187764255No Hit
CAGGAAANAGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG9460.4419631385923521No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT8840.41299726692985117No Hit
CAGGAAANAGCTATGANNCCTTGGGCAAGTTAACAAACCTCTCTGAGCCT8630.4031862458828751No Hit
CAGGAAANNGCTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA8530.3985143310986008No Hit
CAGGAAANNGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA8100.3784250975262211No Hit
CAGGAAANAGCTATGANNCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT7760.3625405872596884No Hit
CAGGAAANNGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT7550.3527295662127123No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT7520.35132799177743No Hit
CAGGAAANAGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG7390.3452545025578734No Hit
CAGGAAANNGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT7270.3396482048167442No Hit
CAGGAAANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG6700.3130182905463804No Hit
CAGGAAANAGCTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA6680.31208390758952553No Hit
CAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC6660.3111495246326707No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG6620.309280758718961No Hit
CAGGAAANNGCTATGANNAGCGTCTCATTCCATAAAGGCCACCATGAAAC6590.30787918428367866No Hit
CAGGAAANNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG6530.30507603541311407No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCTCCCCACCTCCC6370.29760097175827516No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA6190.2891915251465813No Hit
CAGGAAANAGCTATGANNACACCCGGAGCAGTGCAAATCAACTCCCCCAG5950.2779789296643229No Hit
NAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT5850.2733070148800486No Hit
CAGGAAANAGCTATGANNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT5720.26723352566049197No Hit
NAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT5410.2527505898292415No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCCAAACCAG5390.25181620687238665No Hit
CAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT5310.24807867504496717No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA5250.24527552617440257No Hit
CAGGANANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT5220.2438739517391203No Hit
CAGGAAANNGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT5110.23873484547641852No Hit
NAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT4870.2275222499941601No Hit
CAGGAAANAGCTATGANNTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG4820.22518629260202294No Hit
CAGGANANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG4750.2219159522530309No Hit
NAGGANANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG4730.22098156929617604No Hit
NAGGANANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT4630.2163096545119017No Hit
NAGGAAANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC4620.21584246303347426No Hit
CAGGAAANNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC4610.21537527155504685No Hit
NAGGAAANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG4470.20883459085706277No Hit
CAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA4460.20836739937863535No Hit
CAGGAAANAGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT4430.20696582494335306No Hit
CAGGAAANAGCTATGANNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT4390.20509705902964329No Hit
CAGGANANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC4370.20416267607278843No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA4300.20089233572379642No Hit
NAGGANANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC4130.19295008059053No Hit
CAGGAAANAGCTATGANNGCTGCTGACCCAACCCTACCCTGTCCAGCCCC3960.18500782545726366No Hit
CAGGAAANNGCTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA3930.18360625102198136No Hit
CAGGAAACAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3900.18220467658669906No Hit
CAGGAAANNGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG3740.17472961293186012No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT3540.16538578336331144No Hit
CAGGAAACNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3510.16398420892802915No Hit
NAGGANANNGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA3290.1537059964026256No Hit
CAGGANANNGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA3230.150902847532061No Hit
CAGGAAANNGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT3200.14950127309677871No Hit
NAGGAAANNGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA3120.14576374126935926No Hit
CAGGAAACAGCNATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG3020.14109182648508492No Hit
CAGGAANNNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3010.14062463500665748No Hit
CAGGAAANNGCTATGANNCCTTGGGCAAGTTAACAAACCTCTCTGAGCCT3010.14062463500665748No Hit
NAGGAAANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2930.13688710317923802No Hit
NAGGANANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2920.13641991170081058No Hit
CAGGAAANNGCTATGANNCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT2830.13221518839496368No Hit
NAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2820.13174799691653624No Hit
CAGGAAACAGCTATGANCACAGTGTCCCCATCAGGCTCCGGGACAGCATC2820.13174799691653624No Hit
CAGGAAACAGCTATGANCTGTTTTGCCCCTGAAATGAATATTCATCGTCT2760.12894484804597164No Hit
NAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2720.1270760821322619No Hit
CAGGAAANAGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG2640.12333855030484243No Hit
NAGGAAANAGCTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG2640.12333855030484243No Hit
CAGGAAANNGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT2610.12193697586956015No Hit
CAGGANANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2610.12193697586956015No Hit
CAGGAAANAGCTATGANNGCATCACTGAGGGAGGATCCTCCCCGGGCAGA2610.12193697586956015No Hit
CAGGAAANAGCTATGANNGGAGGCTGAGGTGGGAGGATCGCTTGATCCCG2610.12193697586956015No Hit
CAGGAAANNGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG2600.12146978439113272No Hit
CAGGAAANNGCTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA2580.12053540143427784No Hit
CAGGAAANNGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA2570.12006820995585041No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTATGTGCA2560.11960101847742297No Hit
CAGGANANNGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA2540.11866663552056811No Hit
NAGGAAANNGCTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA2530.11819944404214067No Hit
CAGGAAACNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2520.11773225256371322No Hit
NAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2520.11773225256371322No Hit
NAGGAAANAGCTATGANCTGTTTTGCCCCTGAAATGAATATTCATCGTCT2490.11633067812843094No Hit
NAGGAAANAGCTATGANCACAGTGTCCCCATCAGGCTCCGGGACAGCATC2470.11539629517157608No Hit
CAGGAAANNGCTATGANNCTGTGAGGCACCAGAGCACCTCCCCACCTCCC2450.1144619122147212No Hit
CAGGAAANAGCTATGANNAAGTTGGTCAGCCCTGGTGACTCATTGGATAT2440.11399472073629377No Hit
NAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG2430.11352752925786634No Hit
CAGGANANNGCTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA2430.11352752925786634No Hit
CAGGAAANNGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA2420.11306033777943891No Hit
CAGGAAACAGCNATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2420.11306033777943891No Hit
CAGGAANNNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2380.11119157186572917No Hit
CAGGAANNNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2350.10978999743044687No Hit
NAGGAAANAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2310.10792123151673713No Hit
NAGGANANNGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT2300.1074540400383097No Hit
NAGGANANNGCTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA2290.10698684855988227No Hit
CAGGAANNNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG2260.10558527412459996No Hit
CAGGAAACAGCNATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2250.10511808264617253No Hit
CAGGAAACAGCTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG2230.10418369968931768No Hit
CAGGAAANAGCTATGANNGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA2220.10371650821089023No Hit
CAGGAAANNGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA2210.10324931673246279No Hit
NAGGANANNGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT2200.10278212525403536No Hit
NAGGANANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2180.1018477422971805No Hit
CAGGAAACNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2170.10138055081875307No Hit
NAGGANANNGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA2170.10138055081875307No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG2160.10091335934032564No Hit
NAGGAAANNGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT2160.10091335934032564No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG2150.10044616786189821No Hit
CAGGANANNGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT2150.10044616786189821No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)