FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140375
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG20941.491718610863758No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA18921.3478183437221727No Hit
CAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT15881.1312555654496883No Hit
CAGGAAANAGCTATGANNGGGGAGACTGGGCCATACGATAGGCTTAATCC14711.047907390917186No Hit
CAGGAAANAGCTATGANNCCCGTGACAGCTCCTGCAATTACAAAATGGCT11300.804986642920748No Hit
CAGGAAANAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG10980.7821905609973286No Hit
CAGGAAANAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA9750.6945681211041852No Hit
CAGGAAANAGCTATGACCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT9170.6532502226179875No Hit
CAGGAAANAGCTATGACCGGGGAGACTGGGCCATACGATAGGCTTAATCC8160.581300089047195No Hit
CAGGAAANAGCTATGANNAGGACTTGCCCACTCTCCCAGTTACTTGGAAT7510.5349955476402493No Hit
CAGGAAANAGCTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA7450.5307212822796081No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTATGTGCA7210.5136242208370436No Hit
CAGGAAANAGCTATGANNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT7020.5000890471950133No Hit
CAGGAAANAGCTATGANNCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC6980.49723953695458595No Hit
CAGGAAANAGCTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG6940.4943900267141585No Hit
CAGGAAANAGCTATGANNTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG6880.49011576135351737No Hit
CAGGAAANAGCTATGANNTGACAGCACAAGGAACACTGACCTACAGATAA6730.4794300979519145No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG6350.45235975066785394No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT6310.4495102404274265No Hit
CAGGAAANAGCTATGACCCCCGTGACAGCTCCTGCAATTACAAAATGGCT6240.4445235975066786No Hit
CAGGAAACAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG6190.44096170970614423No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA6160.4388245770258237No Hit
CAGGAAANAGCTATGANCAGGCACCAACAGTACAAAGCAGATACAAAGCA6090.4338379341050757No Hit
CAGGAAANAGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC5980.42600178094390023No Hit
CAGGAAANAGCTATGANNTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC5880.4188780053428317No Hit
CAGGAAACAGCTATGACCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT5820.4146037399821906No Hit
CAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC5760.41032947462154945No Hit
CAGGAAANAGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG5670.4039180765805877No Hit
CAGGAAANAGCTATGANNTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC5640.4017809439002672No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT5610.3996438112199466No Hit
CAGGAAANAGCTATGANCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT5570.3967943009795191No Hit
CAGGAAANAGCTATGANCGGGGAGACTGGGCCATACGATAGGCTTAATCC5550.39536954585930545No Hit
CAGGAAANAGCTATGANNGGCAGACAGCCTCTCCTCCTTTCATCACAAAA5480.39038290293855743No Hit
CAGGAAANAGCTATGANNCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG5340.38040961709706145No Hit
CAGGAAACAGCTATGACCGGGGAGACTGGGCCATACGATAGGCTTAATCC5190.3697239536954586No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT5120.3647373107747106No Hit
CAGGAAANAGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC4790.3412288512911843No Hit
CAGGAAANAGCTATGANNCAGGGTGACTCCGATCCTGGAGGACAAGGCGG4740.33766696349065No Hit
CAGGAAANAGCTATGANNGTCAACAGCATACCTGTACACCTGAAATAACT4670.33268032056990204No Hit
CAGGAAANAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA4380.3120213713268032No Hit
CAGGAAANAGCTATGANNCTGGGTGCCGGGTAGAGTGTATACAGGGCTCA4230.30133570792520037No Hit
CAGGAAANAGCTATGANNGGCTGGCACAAAGAACAGCACTGACTGTTTAT4200.29919857524487975No Hit
CAGGAAANAGCTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA4200.29919857524487975No Hit
CAGGAAANAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA4160.29634906500445235No Hit
CAGGAAANAGCTATGACCAGGACTTGCCCACTCTCCCAGTTACTTGGAAT4000.28495102404274264No Hit
CAGGAAANAGCTATGANNTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC3970.2828138913624221No Hit
CAGGAAANAGCTATGANNAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT3920.2792520035618878No Hit
CAGGAAANAGCTATGACCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT3760.2678539626001781No Hit
CAGGAAANAGCTATGANCCCCGTGACAGCTCCTGCAATTACAAAATGGCT3640.2593054318788958No Hit
CAGGAAANAGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC3630.25859305431878893No Hit
CAGGAAACAGCTATGACCCCCGTGACAGCTCCTGCAATTACAAAATGGCT3550.2528940338379341No Hit
CAGGAAANAGCTATGACCTGACAGCACAAGGAACACTGACCTACAGATAA3530.2514692787177204No Hit
CAGGAAANAGCTATGANNCGCTTCTGGGGACTTTGCTCTTGGGCTCTTTT3530.2514692787177204No Hit
CAGGAAANAGCTATGACCCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC3510.2500445235975067No Hit
CAGGAAANAGCTATGANNGGAGAAGCACAGCTTCGGCTCTGGCCCTGGGG3510.2500445235975067No Hit
CAGGAAANAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG3490.24861976847729297No Hit
CAGGAAANAGCTATGANNTTCCTGATGGGATTCAGCCATCAACAGTAGGA3470.24719501335707925No Hit
CAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC3450.24577025823686555No Hit
CAGGAAANAGCTATGANNGCAGCAAGAGACCAATACCGCATTGTAGTCAA3420.24363312555654498No Hit
CAGGAAANAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC3400.24220837043633126No Hit
CAGGAAANAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT3400.24220837043633126No Hit
CAGGAAANAGCTATGANNCCACCCTCATGCCTAACCCCTATTTTCCAAAG3370.2400712377560107No Hit
CAGGAAANAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC3300.23508459483526267No Hit
CAGGAAANAGCTATGANNGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG3290.2343722172751558No Hit
CAGGAAANAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC3270.2329474621549421No Hit
CAGGAAANAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG3250.2315227070347284No Hit
CAGGAAANAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG3240.23081032947462155No Hit
CAGGAAANAGCTATGACCCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG3230.23009795191451468No Hit
CAGGAAANAGCTATGANNAGCAGCTTTCAAACCTAGACACTGGCAGGTGG3200.22796081923419412No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA2920.20801424755120212No Hit
CAGGAAANAGCTATGACCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG2890.20587711487088156No Hit
CAGGAAANAGCTATGANNAAGGACAAGGTGAGGCAGCAAGTGCTGATGTA2850.20302760463045416No Hit
CAGGAAANAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT2840.2023152270703473No Hit
CAGGAAANAGCTATGANNCCTGGACGCAATCACCCAGGCCAGCTAAGTGA2820.2008904719501336No Hit
CAGGAAANAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC2760.19661620658949241No Hit
CAGGAAANAGCTATGACCGGCAGACAGCCTCTCCTCCTTTCATCACAAAA2750.19590382902938555No Hit
CAGGAAANAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT2740.19519145146927872No Hit
CAGGAAANAGCTATGANNAGGCTTTGTGTGTGGGGACTGGAAACTGGGAA2690.19162956366874442No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA2680.19091718610863756No Hit
CAGGAAANAGCTATGANNTGTTGTCTTTCAGGGGCCGCCATGCCTGCCGT2630.1873552983081033No Hit
CAGGAAANAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC2610.1859305431878896No Hit
CAGGAAANAGCTATGANNAAGGACAAGGTGAGGCAGCAGTAATGGACTGG2580.183793410507569No Hit
CAGGAAANAGCTATGANCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA2580.183793410507569No Hit
CAGGAAANAGCTATGACCGTCAACAGCATACCTGTACACCTGAAATAACT2550.18165627782724844No Hit
CAGGAAACAGCTATGACCTGACAGCACAAGGAACACTGACCTACAGATAA2490.1773820124666073No Hit
CAGGAAANAGCTATGANCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG2490.1773820124666073No Hit
CAGGAAANAGCTATGANNCTGGGTGCCGGGTAGAGTGTTGGGAAAGACTG2460.17524487978628672No Hit
CAGGAAANAGCTATGANCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT2440.17382012466607302No Hit
CAGGAAANAGCTATGANNGCATCACTGAGGGAGGATCCTCGGGTCTTACA2420.17239536954585932No Hit
CAGGAAANAGCTATGANCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT2410.17168299198575246No Hit
CAGGAAANAGCTATGANCAGGACTTGCCCACTCTCCCAGTTACTTGGAAT2410.17168299198575246No Hit
CAGGAAANAGCTATGANNAGAAAGAAGAAGCAGAGCAAAAACAGTAGTCT2390.17025823686553873No Hit
CAGGAAACAGCTATGACCCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC2380.16954585930543187No Hit
CAGGAAANAGCTATGANCTTCATACAGAGATACAAAGGCAACTATGTGCA2360.16812110418521817No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTGGCCGTC2340.16669634906500444No Hit
CAGGAAANAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGGCTCTTTT2320.16527159394479074No Hit
CAGGAAANAGCTATGANNAGAAAGAAGAAGCAGAGCAAAAACAATAGTCT2300.16384683882457704No Hit
CAGGAAACAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC2300.16384683882457704No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG2270.16170970614425648No Hit
CAGGAAANAGCTATGANCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG2250.16028495102404272No Hit
CAGGAAANAGCTATGANCCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC2230.15886019590382902No Hit
CAGGAAACAGCTATGACCAGGACTTGCCCACTCTCCCAGTTACTTGGAAT2210.15743544078361532No Hit
CAGGAAANAGCTATGACCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA2190.1560106856634016No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2190.1560106856634016No Hit
CAGGAAANAGCTATGANNGGGCAGGCCGAGGACATTAGAAATTTGGGACG2190.1560106856634016No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT2110.15031166518254677No Hit
CAGGAAANAGCTATGANCTGACAGCACAAGGAACACTGACCTACAGATAA2110.15031166518254677No Hit
CAGGAAANAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT2090.14888691006233304No Hit
CAGGAAACAGCTATGACCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT2080.14817453250222618No Hit
CAGGAAANAGCTATGACCCTGGGTGCCGGGTAGAGTGTATACAGGGCTCA2080.14817453250222618No Hit
CAGGAAANAGCTATGANCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2050.1460373998219056No Hit
CAGGAAACAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG2040.14532502226179875No Hit
CAGGAAANAGCTATGANCCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC2020.14390026714158505No Hit
CAGGAAACAGCTATGACCGGCAGACAGCCTCTCCTCCTTTCATCACAAAA2010.14318788958147818No Hit
CAGGAAANAGCTATGANCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG2010.14318788958147818No Hit
CAGGAAANAGCTATGACCGGCTGGCACAAAGAACAGCACTGACTGTTTAT2010.14318788958147818No Hit
CAGGAAANAGCTATGANCGGCAGACAGCCTCTCCTCCTTTCATCACAAAA2000.14247551202137132No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT2000.14247551202137132No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC1980.14105075690115762No Hit
CAGGAAANAGCTATGANCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC1970.14033837934105076No Hit
CAGGAAACAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC1950.13891362422083703No Hit
CAGGAAANAGCTATGANCCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG1950.13891362422083703No Hit
CAGGAAANAGCTATGANNACAGGGGCCTCTTAAAACTGCATGTGTTCAGC1950.13891362422083703No Hit
CAGGAAANAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC1950.13891362422083703No Hit
CAGGAAANAGCTATGANCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC1950.13891362422083703No Hit
CAGGAAANAGCTATGANNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT1940.1382012466607302No Hit
CAGGAAANAGCTATGANNACCCCTTAAGCCAGAGCCGACTGGCCGTCGTT1930.13748886910062333No Hit
CAGGAAANAGCTATGACCCCACCCTCATGCCTAACCCCTATTTTCCAAAG1900.13535173642030277No Hit
CAGGAAACAGCTATGACCCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG1890.1346393588601959No Hit
CAGGAAANAGCTATGACCGGAGAAGCACAGCTTCGGCTCTGGCCCTGGGG1890.1346393588601959No Hit
CAGGAAANAGCTATGANCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT1890.1346393588601959No Hit
CAGGAAANAGCTATGANCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG1870.1332146037399822No Hit
CAGGAAANAGCTATGACCGCAGCAAGAGACCAATACCGCATTGTAGTCAA1870.1332146037399822No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG1860.13250222617987534No Hit
CAGGAAANAGCTATGANCGTCAACAGCATACCTGTACACCTGAAATAACT1840.13107747105966164No Hit
CAGGAAANAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC1820.1296527159394479No Hit
CAGGAAANAGCTATGACCTTCCTGATGGGATTCAGCCATCAACAGTAGGA1820.1296527159394479No Hit
CAGGAAANAGCTATGACCGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG1810.12894033837934105No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC1800.12822796081923418No Hit
CAGGAAANAGCTATGACCAGCAGCTTTCAAACCTAGACACTGGCAGGTGG1790.12751558325912732No Hit
CAGGAAANAGCTATGANNCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT1730.1232413178984862No Hit
CAGGAAANAGCTATGACCAGGCTTTGTGTGTGGGGACTGGAAACTGGGAA1720.12252894033837934No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT1670.11896705253784505No Hit
CAGGAAANAGCTATGANCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT1660.11825467497773819No Hit
CAGGAAANAGCTATGANNCCCATCCACTAGTCGGGGAGTTACACAAACAG1640.11682991985752449No Hit
CAGGAAANAGCTATGACCTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC1630.11611754229741762No Hit
CAGGAAACAGCTATGACCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG1620.11540516473731077No Hit
CAGGAAANAGCTATGANNGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA1600.11398040961709706No Hit
CAGGAAACAGCTATGACCGTCAACAGCATACCTGTACACCTGAAATAACT1590.11326803205699021No Hit
CAGGAAANAGCTATGANCTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC1570.1118432769367765No Hit
CAGGAAANAGCTATGACCAAGGACAAGGTGAGGCAGCAGTAATGGACTGG1570.1118432769367765No Hit
CAGGAAANAGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1560.11113089937666965No Hit
CAGGAAANAGCTATGANNCTGGGTGCCGGGCAGAGGGTTGGGAAAGACTG1550.11041852181656278No Hit
CAGGAAACAGCTATGANCAGGCACCAACAGTACAAAGCAGATACAAAGCA1540.10970614425645593No Hit
CAGGAAACAGCTATGACCCTGGGTGCCGGGTAGAGTGTATACAGGGCTCA1520.1082813891362422No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA1510.10756901157613534No Hit
CAGGAAANAGCTATGANCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA1490.10614425645592163No Hit
CAGGAAANAGCTATGACCTTCATACAGAGATACAAAGGCAACTGGCCGTC1460.10400712377560106No Hit
CAGGAAANAGCTATGACCAAGGACAAGGTGAGGCAGCAAGTGCTGATGTA1450.10329474621549421No Hit
CAGGAAANAGCTATGACCACAGGGGCCTCTTAAAACTGCATGTGTTCAGC1440.10258236865538736No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)