FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107653
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT38243.55215367894996No Hit
CAGGAAACAGCTATGACCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC33893.1480776197597837No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA33143.078409333692512No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC27132.5201341346734414No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC24122.2405320799234576No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG22832.1207026278877503No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA21672.0129490121037033No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA21201.9692902195015467No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC20951.9460674574791226No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA18871.752854077452556No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG16341.517839725785626No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCTGCCCAACACCC15411.4314510510622092No Hit
CAGGAAACAGCTATGACCTGAAGTGGCTCCATCCTTCCATTTGTTTATAT15151.4072993785588883No Hit
CAGGAAACAGCTATGACCTGCCCAGTTGTTTCCAGAACCTTGTTTTTGAC13051.2122281775705275No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCAGGAAACAGCT12571.1676404744874735No Hit
CAGGAAACAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT12331.1453466229459468No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG12261.138844249579668No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC11641.0812517997640567No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA11541.0719626949550871No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT11321.0515266643753542No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA11231.0431664700472816No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCAGAGCCACC11161.0366640966810028No Hit
CAGGAAACAGCTATGACCGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC10660.990218572636155No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG10410.9669958106137312No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG10360.9623512582092464No Hit
CAGGAAACAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG9920.9214791970497803No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATGAGTTAGCCAGC9540.886180598775696No Hit
CAGGAAACAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG9370.8703891206004477No Hit
CAGGAAACAGCTATGACCTGGCATCCAGGGCCTATTTGGTTCCTATTGAG9330.8666734786768598No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT9300.863886747234169No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC8470.7867871773197217No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAGGGGACATGGCC8400.780284803953443No Hit
CAGGAAACAGCTATGACCGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG8280.7691378781826795No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT8250.7663511467399886No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGAGCCT7320.6799624720165718No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCAGGAAACAGCT6800.6316591270099301No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTGGCAGCTGCAC6690.6214411117200636No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCCCGTGTCAAGC6670.6195832907582697No Hit
CAGGAAACAGCTATGACCGATGTAGCAGTGCTCTGGAGTCCTCCCTTGCT6290.5842846924841852No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCAGCTCCGA6120.5684932143089371No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC6110.56756430382804No Hit
CAGGAAACAGCTATGACCTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG6070.5638486619044523No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC5840.5424837208438222No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAATGAAACTTGGTT5840.5424837208438222No Hit
CAGGAAACAGCTATGACCGATGTAGCAGTGCTCTGGAGGGTGCTTGTCCC5800.5387680789202345No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT5750.5341235265157497No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCAGCTCCCCAGG5270.4895358234326958No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCGCTCTCCTCCC5030.46724197189116884No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCCAGCTAAGTGA4830.4486637622732297No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCGCCCAACACCC4620.4291566421743937No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGGCTCTTTT4590.4263699107317028No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTACTGGCATGAA4540.421725358327218No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC4510.41893862688452715No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCTTCCCATCTCA4350.40407605919017586No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA4150.3854978495722367No Hit
CAGGAAACAGCTATGACCTCCAGCTGCTCCATCTTGTCTACCTCCCGGCC4050.37620874476326716No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC4000.3715641923587824No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCCCTTCCTTACCT3940.3659907294734006No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC3780.3511281617790493No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCTATTTTCCAAAG3340.3102561006195833No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATTCTCCTCACACC3310.30746936917689244No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGCCTACGACGTAG3210.2981802643679229No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATTGCCTCATGTCC3020.2805309652308807No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATTCTCCATCTCCA2940.27309968138370505No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTTGGCATGAAGG2860.26566839753652943No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTACATCAAATGC2660.24709018791859028No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCGGGTCTCCCCA2630.2443034564758994No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTCCATTCT2570.2387299935905177No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACAGAGGAGCCGGT2530.23501435166692988No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAACACACACACAC2520.23408544118603292No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTACTCCCAACCA2510.23315653070513595No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTTCATTCT2500.23222762022423898No Hit
CAGGAAACAGCTATGACCTTGGACAGCACCTTGAATGCTTTCAGGGAAGC2490.23129870974334202No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACGGCCCCTCTGCC2480.23036979926244508No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCTCTGCAGCTAGC2260.20993376868271205No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCAGGGCCACC2260.20993376868271205No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCGCAGTGGGGCCA2200.2043603057973303No Hit
CAGGAAACAGCTATGACCACCCAGTTAGAGCCATGGTTCCACTAGACTCT2150.1997157533928455No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT2080.19321338002656682No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTATTCCCAACCA1890.17556408088952466No Hit
CAGGAAACAGCTATGACCAACACCCTTGGCCTCCCTCACTGGCCGTCGTT1870.17370625992773076No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCCAGGAAACAGCA1800.16720388656145208No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATCCACCTGCTCTG1780.16534606559965814No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCACGGTGGCTCAT1710.15884369223337946No Hit
CAGGAAACAGCTATGACCGCTAATGCTTACTCTTTGCGTGAGTACTGGTT1680.1560569607906886No Hit
CAGGAAACAGCTATGACCCCCATCCACTAGTCGGGGAGTTACACAAACAG1620.15048349790530688No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTGGCATGAAGGA1610.1495545874244099No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAATG1590.14769676646261598No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCCCCGAGGGGAG1520.14119439309633733No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGAAGAATCGC1480.13747875117274946No Hit
CAGGAAACAGCTATGACCCTGGGCCCCAAAGCAAAGGACTCTTCCCAGTG1390.12911855684467688No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATTGGAAGTGAAGA1370.12726073588288297No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGTTGCAGCCAAGG1350.12540291492108907No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCCCCTGCCTACC1340.1244740044401921No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGAAGAATCAC1300.12075836251660428No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCAGGAAACAGCTA1230.11425598915032557No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGT1180.10961143674584081No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCCACCTCTGCAG1170.10868252626494385No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)