FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115412
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNTGGAAACCCATGCCATGCACTCAACACTACAA7450.6455134648043531No Hit
NNGGANNNNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA5690.49301632412574087No Hit
NNGGANNNNGNTATGNNNGCTTCTATATGAGCGCATCTCACCTGAAGTTG5160.44709388971684055No Hit
NNGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATTCATCGTCT4710.40810314352060445No Hit
NNGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT4490.3890410009357779No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC4120.356981942952206No Hit
NNGGANNNNGNTATGANNGCTTCTATATGAGCGCATCTCACCTGAAGTTG3920.3396527224205455No Hit
NNGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3890.33705333934079645No Hit
NNGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA3650.3162582747028039No Hit
NNGGANNNNGNTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT3570.30932658649013967No Hit
NNGGANNNNGNTATGNNNAAGGACAAGGTGAGGCAGCACCCCACCCTGCT3550.30759366443697367No Hit
NAGGANNNNGNTATGNNNTGGAAACCCATGCCATGCACTCAACACTACAA3420.2963296710913943No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT3380.29286382698506225No Hit
NNGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA3350.29026444390531314No Hit
NNGGANNNNGNTATGNNNTGGGGATGAGGACAGCAGGGCAGTCACCATCT3170.27466814542681867No Hit
NNGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC3000.2599383079749073No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2970.2573389248951582No Hit
NNGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGGACACTGCCCAC2920.2530066197622431No Hit
NNGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT2750.2382767823103317No Hit
NNGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC2640.2287457110179184No Hit
NNGGANNNNGNTATGANNAAGGACAAGGTGAGGCAGCACCCCACCCTGCT2640.2287457110179184No Hit
NNGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT2630.22787924999133538No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCAGGAAACAGCT2530.21921463972550517No Hit
NAGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2510.2174817176723391No Hit
NNGGANNNNGNTATGNNNGGGGAGACTGGGCCATACGATAGGCTTAATCC2510.2174817176723391No Hit
NNGGANNNNGNTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA2500.21661525664575607No Hit
NNGGANNNNGNTATGNNNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT2480.21488233459259No Hit
NNGGANNNNGNTATGNNNACCTTTACCCAAATAGCTACCACCAGTGCCTT2420.20968356843309188No Hit
NNGGANNNNGNTATGNNNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC2410.20881710740650886No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC2360.20448480227359375No Hit
NNGGANNNNGNTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA2340.20275188022042767No Hit
NAGGANNNNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA2330.20188541919384465No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2320.20101895816726162No Hit
NNGGANNNNGNTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC2310.2001524971406786No Hit
NNGGANNNNGNTATGNNNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC2270.19668665303434651No Hit
NNGGANNNNGNTATGNNNTGACTGAAAGGCTTTTCCACGTGCACTGCTGG2270.19668665303434651No Hit
NNGGANNNNGNTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT2270.19668665303434651No Hit
NNGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCAGGAAACAGCT2220.19235434790143138No Hit
NNGGANNNNGNTATGANNTCCCTTAAACTTGGGATTGCTCCATTGCACTC2150.18628912071535023No Hit
NNGGANNNNGNTATGNNNCATGTGCCCCAGTAGAGAGCACAGGAAACAGC2150.18628912071535023No Hit
NNGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGACACTGCCCACC2130.18455619866218417No Hit
NNGGANNNNGNTATGANNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT2120.18368973763560115No Hit
NNGGANNNNGNTATGNNNGCATCACTGAGGGAGGATCCTGTGGGGGAGCC2120.18368973763560115No Hit
NAGGANNNNGNTATGNNNGCTTCTATATGAGCGCATCTCACCTGAAGTTG2110.18282327660901812No Hit
NNGGANNNNGNTATGNNNGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC2110.18282327660901812No Hit
NNGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGGGCACGGGAACA2080.18022389352926907No Hit
NAGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1960.16982636121027275No Hit
NNGGANNNNGNTATGNNNACCTGGCAGCCCCAAATCTGCGGGTGACCACG1960.16982636121027275No Hit
NNGGANNNNGNTATGANNACCTTTACCCAAATAGCTACCACCAGTGCCTT1890.1637611340241916No Hit
NNGGANNNNGNTATGNNNCAGGGTGACTCCGATCCTGGAGCCAACGGTGC1890.1637611340241916No Hit
NNGGANNNNGNTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC1860.16116175094444252No Hit
NNGGANNNNGNTATGNNNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1840.15942882889127646No Hit
NNGGANNNNGNTATGANNTGACTGAAAGGCTTTTCCACGTGCACTGCTGG1770.1533636017051953No Hit
NNGGANNNNGNTATGNNNTCCTCTTCATTTTCACGGTGCCATTCCGATTC1770.1533636017051953No Hit
NNGGANNNNGNTATGNNNCCTGGACGCAATCACCCAGGCTGCAGTGCGGT1710.14816483554569715No Hit
NNGGANNNNGNTATGNNNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG1710.14816483554569715No Hit
NAGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC1690.14643191349253112No Hit
NNGGANNNNGNTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC1660.14383253041278204No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC1660.14383253041278204No Hit
NNGGANNNNGNTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT1610.1395002252798669No Hit
NNGGANNNNGNTATGANNCATGTGCCCCAGTAGAGAGCACAGGAAACAGC1610.1395002252798669No Hit
NNGGANNNNGNTATGNNNTCCTGCCCCACCCTGATACAGGTCACTGTGGA1600.1386337642532839No Hit
NAGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1600.1386337642532839No Hit
NNGGANNNNGNTATGNNNGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG1590.13776730322670086No Hit
NNGGANNNNGNTATGANNGGTAGCAACGTCCCACCCAGGGCACGGGAACA1570.1360343811735348No Hit
NNGGANNNNGNTATGANNGGGGAGACTGGGCCATACGATAGGCTTAATCC1550.13430145912036875No Hit
NNGGANNNNGNTATGNNNCCCAAACCTGCGGAGGCACACAGCTGGGAGGC1550.13430145912036875No Hit
NNGGANNNNGNTATGANNCCTGGACGCAATCACCCAGGCTGCAGTGCGGT1530.13256853706720273No Hit
NNGGANNNNGNTATGANNACCTGGCAGCCCCAAATCTGCGGGTGACCACG1520.1317020760406197No Hit
NAGGANNNNGNTATGNNNAAGGACAAGGTGAGGCAGCACCCCACCCTGCT1500.12996915398745365No Hit
NAGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1500.12996915398745365No Hit
NNGGANNNNGNTATGANNGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC1490.12910269296087062No Hit
NNGGANNNNGNTATGANNGCATCACTGAGGGAGGATCCTGTGGGGGAGCC1480.1282362319342876No Hit
NNGGANNNNGNTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1430.12390392680137247No Hit
NNGGANNNNGNTATGANNCAGGGTGACTCCGATCCTGGAGCCAACGGTGC1420.12303746577478945No Hit
NNGGANNNNGNTATGNNNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT1420.12303746577478945No Hit
NNGGANNNNGNTATGANNCCCAAACCTGCGGAGGCACACAGCTGGGAGGC1420.12303746577478945No Hit
NAGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA1420.12303746577478945No Hit
NNGGANNNNGNTATGNNNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG1390.12043808269504036No Hit
NNGGANNNNGNTATGNNNCAGGGTGACTCCGATCCTGGAGGACAAGGCGG1380.11957162166845736No Hit
NAGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1370.11870516064187434No Hit
NNGGANNNNGNTATGNNNATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC1350.11697223858870827No Hit
NAGGANNNNGCTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA1350.11697223858870827No Hit
NAGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA1340.11610577756212526No Hit
NAGGANNNNGNTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1340.11610577756212526No Hit
NAGGANNNNGNTATGANNGCTTCTATATGAGCGCATCTCACCTGAAGTTG1340.11610577756212526No Hit
NAGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGGACACTGCCCAC1330.11523931653554223No Hit
NNGGANNNNGNTATGANNCACGACACCCCACCTCTTGGACACTGCCCACC1320.1143728555089592No Hit
NNGGANNNNGNTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG1310.11350639448237618No Hit
NNGGANNNNGNTATGNNNAACCAACACTGCTGCTAATTGTGTTTGTGTCC1280.1109070114026271No Hit
NNGGANNNNGNTATGANNATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC1270.1100405503760441No Hit
NNGGANNNNGNTATGANNTCCTGCCCCACCCTGATACAGGTCACTGTGGA1250.10830762832287803No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACCCTTTGTCCA1240.107441167296295No Hit
NNGGANNNNGNTATGNNNCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT1240.107441167296295No Hit
NAGGANNNNGNTATGNNNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC1240.107441167296295No Hit
NNGGANNNNGNTATGANNGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG1220.10570824524312897No Hit
NNGGANNNNGNTATGNNNTGCTTTGGGGCCATGGAGTTGAGCTGTTGAGA1220.10570824524312897No Hit
NAGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCAGGAAACAGCT1220.10570824524312897No Hit
NNGGANNNNGNTATGNNNTCCTCTTCATTTTCACGGTGCCATTCTGATTC1220.10570824524312897No Hit
NNGGANNNNGNTATGNNNCTCGTGCAGTCTGGTGTCCGCAGGAAACAGCT1210.10484178421654594No Hit
NAGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC1190.1031088621633799No Hit
CAGGAAANAGCTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA1190.1031088621633799No Hit
NAGGANNNNGNTATGNNNTGGGGATGAGGACAGCAGGGCAGTCACCATCT1180.10224240113679688No Hit
NAGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT1180.10224240113679688No Hit
NNGGANNNNGNTATGNNNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA1170.10137594011021384No Hit
NAGGANNNNGNTATGNNNTGACTGAAAGGCTTTTCCACGTGCACTGCTGG1160.10050947908363081No Hit
NAGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1160.10050947908363081No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)