FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112357
Sequences flagged as poor quality0
Sequence length300
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTCACCATTTGGTG24882.214370266205043No Hit
TGTAAAACGACGGCCAGTTCCATTACTCTACACAAGCAGGGTGGGGTGCT23582.0986676397554227No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGACAGGGTCAGT21531.9162134980464058No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA21251.8912929323495642No Hit
TGTAAAACGACGGCCAGTGGCTCCCCAGCTGTCTCTCTTGTAAAACGACG21061.8743825484838506No Hit
TGTAAAACGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT18261.6251768915154376No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCAGCAGGATGGAG17801.5842359621563409No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGGGCCCCGGGAGC17491.5566453358491237No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGACGGCACTGGTG16831.497904002420855No Hit
TGTAAAACGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTCTGGCATGG15861.411572042685369No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA15791.4053419012611585No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT15681.395551679023114No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGGTCTGTGCGG15191.3519406890536414No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG14011.2469183050455246No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA13851.2326779817901865No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCCCTCGGAGCTG13471.1988572140587592No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG12841.142785941240866No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTCCATTTGGTGTG12411.1045150724921455No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGTCCAACAATGGCCTG12391.1027350320852285No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGACATTCCTGCAA12381.1018450118817698No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG12281.0929448098471835No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC11721.0431036784535008No Hit
TGTAAAACGACGGCCAGTTCCTTCTTGGGTGGGTATTTTGCAACTGGGTA11301.0057228299082388No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGAAGCCTCAACCC11150.9923725268563597No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT10710.9532116379041805No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAGTGGGAGTCTGGCTAC10260.9131607287485426No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC9820.8739998397963633No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATCACGGTGGT9540.8490792740995221No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG9170.8161485265715532No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG8840.7867778598574188No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGCCAGTTAACCA8740.7778776578228326No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC8500.7565171729398257No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC8000.7120161627668948No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCTGTGGGAGACCCAGC7930.7057860213426845No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT7900.7031159607323086No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTCTAGCCTGGGCA7770.6915456980873467No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT7530.6701852132043398No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGAGCTCAAGGGT7500.6675151525939639No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTTGGGAAGGGA7460.6639550717801295No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCTGCAGACAGGAT7210.6417045666936639No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC7200.6408145464902053No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGCATCCTGGGTGAC6950.6185640414037399No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC6820.6069937787587778No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAGGATGCTGAGTGGGAG6760.6016536575380261No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCGGTCATAGCCTCT6510.5794031524515606No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTGTAAAACGACGG6450.5740630312308089No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGCACGGCACTGGT6400.5696129302135158No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCGAGCAGGTGGC6210.5527025463478021No Hit
TGTAAAACGACGGCCAGTTGGCCCACTGTGCTAGCCTACAGGAACCATGG6040.5375722028890055No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGTTGCTGTGCCCG5850.5206618190232919No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTAGCCTCTCCATT5840.5197717988198333No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCCAGGGGGCGTCCCGG5830.5188817786163746No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT5730.5099815765817883No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC5490.4886210916987816No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGGTGGCCCTTC5360.47705082905381957No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCACTAGGAGAG5200.4628105057984816No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCCTGAGG5100.45391030376389546No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGGTGACAGAGTGTG5070.4512402431535196No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC4920.4378899401016403No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGGTTGGAGGTG4920.4378899401016403No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCCCACAGACCCC4890.4352198794912644No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTCCTCACTCGGAT4820.4289897380670541No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCCCCCCAGGATGG4760.4236496168463024No Hit
TGTAAAACGACGGCCAGTTCCATTACTCTACACAAGCAGGGTGCTCCACA4640.41296937440479903No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG4590.40851927338750593No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC4570.40673923298058867No Hit
TGTAAAACGACGGCCAGTGCCCAAGGTTGGTGAGATGGGGCCAGGTCATA4550.40495919257367136No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACACGGCAGGAGA4530.40317915216675415No Hit
TGTAAAACGACGGCCAGTGGTCCCACTCTGGCTTTCCCTCCTCCATGGCT4400.3916088895217921No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGTACGGCACTGGT4360.38804880870795766No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGTAGAGATGGGGT4330.3853787480975818No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCCAGGATGGAGGG3980.35422804097653016No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAGGGTCAGTGGGAGTCT3960.35244800056961295No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGAAGCCCAGTCAGG3920.3488879197557785No Hit
TGTAAAACGACGGCCAGTTGGCCCACTGTGCTAGCCTAGGAACCATGGCT3850.34265777833156813No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGAGCTCAACCGT3790.3373176571108164No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGCCCAGCACGACG3770.3355376167038992No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA3760.33464759650044057No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGTGTAAAACGACG3750.33375757629698194No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGCTGGGGCCGGA3730.33197753589006473No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGACCCGTGTCCG3730.33197753589006473No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGTGGGGTCACCCC3690.32841745507623027No Hit
TGTAAAACGACGGCCAGTTCCATTACTCTACACAAGCAGGGTGCTGCCTC3570.3177372126347268No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCGTGGGTC3550.31595717222780956No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG3490.3106170510070579No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGACTCCTAAGGGAG3420.30438690958284753No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC3270.2910366065309683No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAGTAGGAGAG3250.289256566124051No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGCCTAGCACGACG3110.2767962832756304No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAGGAGAGTGA3100.27590626307217175No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGAGGCTTTTTGTTCCA3050.2714561620548786No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG2970.26433600042720967No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG2940.26166593981683384No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGAAAGCCTCAGGCA2910.258995879206458No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACACTGCCGAGTG2810.2500956771718718No Hit
TGTAAAACGACGGCCAGTTGGGGTCTCCTGGTCCCATTCGAGCATGCTGC2640.23496533371307526No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAAGGAGAGTG2600.2314052528992408No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGGGGGTGCCTCAA2590.2305152326957822No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC2500.22250505086465464No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAGTGGGACTCCAAGTGG2420.21538488923698568No Hit
TGTAAAACGACGGCCAGTGGTCCCACTCTGGCTTTCCCCTCGCCTGCATG2400.21360484883006844No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAAGCCCCAGACCC2360.21004476801623398No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACTGCCTTCCAGCATTT2330.20737470740585812No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCTGGAGACCCAGCGCG2310.20559466699894088No Hit
TGTAAAACGACGGCCAGTGCCAATCCTGAGGCCATCAACACAAGGACTGG2270.2020345861851064No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAGTG2240.19936452557473053No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCCCTCAGAGCTG2210.1966944649643547No Hit
TGTAAAACGACGGCCAGTTCCATTACTCTACACAAGCAGGGTCTGCTCCT2170.19313438415052023No Hit
TGTAAAACGACGGCCAGTGGCTCTATATCAGGGCCCGCCGGTCATAGCTG2150.191354343743603No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG2130.18957430333668576No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAGCGAC2110.1877942629297685No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGTCCCAGCTACT2110.1877942629297685No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGATAGGGCCAGGC2090.18601422252285127No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAGGGGAGGGCTACAGTC2050.1824541417090168No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGACCGTGTCCGA1990.17711402048826508No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC1880.16732379825022028No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA1860.16554375784330305No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTTGAATTCC1850.16465373763984442No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGGGCCGGCCCGT1770.1575335760121755No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCAGGATGGAGGGG1750.15575353560525826No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCACACAGACCCC1740.15486351540179963No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT1730.153973495198341No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG1610.14329325275683757No Hit
TGTAAAACGACGGCCAGTGGCTCCCCAGCTGTCTCTCTTCAGCTCTATAT1600.14240323255337894No Hit
TGTAAAACGACGGCCAGTGCCCAAGGTTGGTGAGATGGGAACCACCCAGC1520.13528307092571001No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCATCTTGGGTGCCACAGG1450.12905292950149969No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGCCTCGGAGGC1440.12816290929804108No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGGGCCGGTCCGT1400.12460282848420659No Hit
TGTAAAACGACGGCCAGTTGCCTTGAAGATTTTCTGTAGGATAAAGGGGC1380.12282278807728936No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGTAAAACGACGGC1370.12193276787383074No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCAGCGTCCAC1360.12104274767037213No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGACCAACAATGGCCTG1320.11748266685653763No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC1230.10947248502541007No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTAAAACGACGG1220.10858246482195145No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTTAATACATTAGT1200.10680242441503422No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCCT1180.10502238400811699No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC1150.10235232339774114No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGCAGTGGCACCAACAA1140.10146230319428252No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)