FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112357
Sequences flagged as poor quality0
Sequence length300
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATGGTGAGCCCTGC25002.225050508646546No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCACCCCACCCTGCT24482.178769458066698No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACCCTGTCTCTCTAGC22552.0069955587991846No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC22141.9705047304573813No Hit
CAGGAAACAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG20211.798730831189868No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG19041.6945984673852097No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGCCCGGACGAGGA18131.6136066288704751No Hit
CAGGAAACAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT17281.5379549115764928No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCCATGCTCACCT17071.5192644873038619No Hit
CAGGAAACAGCTATGACCGGGACCAGTCCAATAACCCCTGAGGGGTATAT15611.3893215375989036No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGCACAGACCCCACA15521.381311355767776No Hit
CAGGAAACAGCTATGACCGGCGGGCCCTGATATAGAGCCACTGGCCGTCG15311.362620931495145No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA15231.355500769867476No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT14971.332360244577552No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC13901.2371280828074798No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA13721.2211077191452246No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCAGCTCCGA13691.2184376585348486No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG13681.2175476383313901No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGCAGGAAACAGCT12981.1552462240892867No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTCCCCAACCATAG12821.1410059008339488No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGACACTGCAGCCTGGG12781.1374458200201145No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAGGCACTACGT12601.1214254563578594No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATGGAGCCCTGCAG12521.1143052947301904No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC12101.0769244461849283No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCTTCCCATCTCA11811.0511138602846284No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTGGCAGCTGCAC10470.9318511530211735No Hit
CAGGAAACAGCTATGACCGAGGCGCAGGTCACAGTGAGACCAATACAGAG10040.8935802842724531No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC9970.8873501428482428No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACCACCGTGATCTCTC9880.879339961017115No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAACTGGCCAGCCTG9200.8188185871819291No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT8730.776987637619374No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC8580.7636373345674947No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG8380.7458369304983223No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCCCACAGAGAGCAC8090.7200263445980224No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA8000.7120161627668948No Hit
CAGGAAACAGCTATGACCAGACAGGGTCTTGCTATGTTGCCCAGGCTAGA7960.7084560819530603No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGTCTGCAGCCTGG7870.7004459001219328No Hit
CAGGAAACAGCTATGACCCACCGCTGTCCCTTCCCAAGGACTATGCTGAA7840.697775839511557No Hit
CAGGAAACAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT7490.6666251323905052No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGATGCCACG7190.6399245262867467No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCAGAGCCACC7070.6292442838452433No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGCATCCTGG6810.6061037585553192No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTATGACCGCCCACC6460.5749530514342676No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTGCCATGCTCACC6400.5696129302135158No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT6320.562492768585847No Hit
CAGGAAACAGCTATGACCACCCAGTTAGAGCCATGGTTCCTGTAGGCTAG6070.5402422634993814No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGCCGGGACGCCCC6040.5375722028890055No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTAGCCCACCGCAAA6030.5366821826855469No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT5950.529562021057878No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA5910.5260019402440436No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGGCACAGCAACTC5810.5171017382094574No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG5560.4948512331229919No Hit
CAGGAAACAGCTATGACCGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG5430.48328097047802987No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGAAGGGCCACCCCA5380.4788308694607368No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTAGTGGCATGAA5360.47705082905381957No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAACTGGCCGTCGTT5330.4743807684434436No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACCCCGTTAGCTGC5230.4654805664088575No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCAAGCCTGACTG5180.4610304653915644No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGTCTGTGGGCGGA5110.4548003239673541No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTCAGGAATCATGC5110.4548003239673541No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGGGGGCCTGGCT5080.4521302633569782No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCACGCCCGGCCT5080.4521302633569782No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGACTGGCCGTCGTT5040.44857018254314374No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATCCGAGTGAGGAG4930.4387799603050989No Hit
CAGGAAACAGCTATGACCTGGCCCCAGTGTTGAGAGGTCAGGAAAACAGC4890.4352198794912644No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCGTGTTCTCACTC4810.42809971786359546No Hit
CAGGAAACAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG4650.4138593946082576No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT4630.4120793542013404No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTACCATGCTCACC4510.4013991117598369No Hit
CAGGAAACAGCTATGACCGGCCTTCTCTGCATTATCTCGCTAATCTCGAG4350.38715878850449903No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAAACCCCATCTCTA4350.38715878850449903No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT4340.38626876830104045No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGAGCCT4330.3853787480975818No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGCCTGGCTTGTC4050.36045818240074046No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGGCCCCAGCCACAG3930.34977793995923706No Hit
CAGGAAACAGCTATGACCACCCAGTTAGAGCCATGGTTCCTAGGCTAGCA3930.34977793995923706No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTGACCCTGGCAG3900.3471078793488612No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGTTGAGCTCCTCA3880.345327838941944No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCGTGCTGGGCCA3880.345327838941944No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC3750.33375757629698194No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGACAAGTGCATGG3670.326637414669313No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGGTCCTCAGCCAC3600.32040727324510265No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCACCCTGCTTGTGT3590.319517253041644No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCAGGATAAGATGTG3560.3168471924312682No Hit
CAGGAAACAGCTATGACCGAAAGGCTTCCCCAGGAGCCTCCCTTAGGAGT3550.31595717222780956No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTACTGGCATGAA3520.3132871116174337No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTGGCATGAAGGA3310.29459668734480277No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCGTGCTAGGCCA3170.28213640449638205No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGAACAAAAAGCCTCAC3070.27323620246179586No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAACCAGTGAC3000.26700606103758556No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTTGGCATGAAGG2880.25632581859608217No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG2790.24831563676495455No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACCCTTTGTCCA2740.24386553574766148No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCAGCTCTGA2620.23318529330615803No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCACCCCCCCATC2530.2251751114750305No Hit
CAGGAAACAGCTATGACCTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG2520.22428509127157187No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTAGGGTCTGGGGC2460.21894497005082014No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTACATCAAATGC2420.21538488923698568No Hit
CAGGAAACAGCTATGACCGGCCTTCTCTGCATTATCTCGCTTGCATGCAG2380.2118248084231512No Hit
CAGGAAACAGCTATGACCTCCAGCCAGCCAGTCCTTGTGTTGATGGCCTC2380.2118248084231512No Hit
CAGGAAACAGCTATGACCTCCCTTAAACTTGGGATTGCTCCACTGCACTC2360.21004476801623398No Hit
CAGGAAACAGCTATGACCCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG2340.20826472760931675No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTTGGAGTCCCAC2340.20826472760931675No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATCCAGCAGCAGAG2250.20025454577818916No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCCAGAGAGCACCCG2230.19847450537127193No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGC2230.19847450537127193No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCAGAGGGAGCTAGG2130.18957430333668576No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT2030.18067410130209954No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTTGACTGTAGCC2030.18067410130209954No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCAGCCACCACCACGTACCACT1970.17533398008134785No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGTCCTCAGCCACC1910.16999385886059612No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCCTGGCTTGTCC1900.16910383865713752No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGTCTGTGTGCGGA1900.16910383865713752No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAGTAGTCT1860.16554375784330305No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAAACTCCTGAGATC1790.15931361641909272No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGGCCGGCCCCTCA1640.14596331336721344No Hit
CAGGAAACAGCTATGACCTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG1600.14240323255337894No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACTACAGAGTTAA1580.14062319214646174No Hit
CAGGAAACAGCTATGACCGGCGGGCCCTGATATAGAGCTGAAGAGAGACA1550.13795313153608588No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTTTGGCCCTGTG1480.13172299011187555No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGACCGGCCCCTCA1450.12905292950149969No Hit
CAGGAAACAGCTATGACCTGCCCAATAGTTCTTGCCTCCATCTGCCCCTT1420.12638286889112382No Hit
CAGGAAACAGCTATGACCTCCCTTAAACTTGGGATTGCTCCATTGCACTC1370.12193276787383074No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGAGGCCCACAGGCA1360.12104274767037213No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGTCACTGCAGCCTGGG1360.12104274767037213No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGAGATGAATTTGA1350.12015272746691351No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAATAGTCT1290.11481260624616178No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA1250.11125252543232732No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCAGAATAAGATGTG1150.10235232339774114No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGTGCCACTGCACTGCA1150.10235232339774114No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)